UBE1L (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  UBA7
Gene Name:  ubiquitin-like modifier activating enzyme 7
Original Clone ID:
FLH165254.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00039393
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3036nts
Open reading frame: 1 to 3036

ATGGATGCCCTGGACGCTTCGAAGCTACTGGATGAGGAGCTGTATTCAAGACAGCTGTAT
GTGCTGGGCTCACCTGCCATGCAGAGGATTCAGGGAGCCAGGGTCCTGGTGTCAGGCCTG
CAGGGCCTGGGGGCCGAGGTGGCCAAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACT
CTGCATGATCCCCACCCCACCTGCTGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAG
CAGGACTTGGAAAGGAGCAGAGCCGAGGCCTCTCAAGAGCTCTTGGCTCAGCTCAACAGA
GCTGTCCAGGTCGTCGTGCACACGGGTGACATCACTGAGGACCTGCTGTTGGACTTCCAG
GTGGTGGTGCTGACTGCTGCAAAGCTGGAGGAGCAGCTGAAGGTGGGCACCTTGTGTCAT
AAGCATGGAGTTTGCTTTCTGGCGGCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGT
GACTTTGGTGAGGACTTCACTGTGCAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCC
ATCCAGCACATCTCCCAGGGCTCCCCTGGCATTCTCACTCTGAGGAAAGGGGCCAATACC
CACTACTTCCGTGATGGAGACTTGGTGACTTTCTCGGGAATTGAGGGAATGGTTGAGCTC
AACGACTGTGATCCCCGGTCTATCCACGTGCGGGAGGATGGGTCCCTGGAGATTGGAGAC
ACAACAACTTTCTCTCGGTACTTGCGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAG
ACTGTGAGACATAAGTCCCTGGACACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGC
TCCCAGGAAGTTCACCATGCCCACTGCCTGCATCAGGCCTTCTGTGCACTGCACAAGTTC
CAGCACCTCCATGGCCGGCCACCCCAGCCCTGGGATCCTGTTGATGCAGAGACTGTGGTG
GGCCTGGCCCGGGACCTGGAACCACTGAAGCGGACAGAGGAAGAGCCACTGGAAGAGCCA
CTGGATGAGGCCCTAGTGCGGACAGTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATG
GTGGCCATGCTGGGTGCAGTAGCTGCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTC
ATGCCTCTGGACCAGTGGCTTTACTTTGATGCCCTCGATTGTCTTCCGGAAGATGGGGAG
CTCCTTCCCAGTCCTGAGGACTGTGCCCTGAGAGGCAGCCGCTATGATGGGCAAATTGCA
GTGTTTGGGGCTGGTTTTCAGGAGAAACTGAGACGCCAGCACTACCTCCTGGTGGGCGCT
GGTGCCATTGGTTGTGAGCTGCTCAAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAAC
AGCGGGGGCTTGACTGTTGTTGACATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAG
TTCCTCTTCAGGTCCCAGGACGTTGGTAGACCCAAGGCAGAGGTGGCTGCAGCAGCTGCC
CGGGGCCTGAACCCAGACTTACAGGTGATCCCGCTCACCTACCCACTGGATCCCACCACA
GAGCACATCTATGGGGATAACTTTTTCTCCCGTGTGGATGGTGTGGCTGCTGCCCTGGAC
AGTTTCCAGGCCCGGCGCTATGTGGCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTG
GAGGCAGGCACATCGGGCACCTGGGGCAGTGCTACAGTATTCATGCCACATGTGACTGAG
GCCTACAGAGCCCCTGCCTCAGCTGCAGCTTCTGAGGATGCCCCCTACCCTGTCTGTACC
GTGCGGTACTTCCCTAGCACAGCCGAGCACACCCTGCAGTGGGCCCGGCATGAGTTTGAA
GAACTCTTCCGACTGTCTGCAGAGACCATCAACCACCACCAACAGGCACACACTTCCCTG
GCAGACATGGATGAGCCACAGACACTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGA
GTGCGTCCACAGAACTGGCAAGACTGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGC
TTTCATTATGGCATCAAACAGCTGCTGAGGCACTTCCCACCTAATAAAGTGCTTGAGGAT
GGAACTCCCTTCTGGTCAGGTCCCAAACAGTGTCCCCAGCCCTTGGAGTTTGACACCAAC
CAAGACACACACCTCCTCTACGTACTGGCAGCTGCCAACCTGTATGCCCAGATGCATGGG
CTGCCTGGCTCACAGGACTGGACTGCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCT
GACCCCCAACAGATGGCCCCCATCTTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCT
GAGTTTGGCCCTGAGCAGCAGAAGGAACTGAACAAAGCCCTGGAAGTCTGGAGTGTGGGC
CCTCCCCTGAAGCCTCTGATGTTTGAGAAGGATGATGACAGCAACTTCCATGTGGACTTT
GTGGTAGCGGCAGCTAGCCTGAGATGTCAGAACTACGGGATTCCACCGGTCAACCGTGCC
CAGAGCAAGCGAATTGTGGGCCAGATTATCCCAGCCATTGCCACCACTACAGCAGCTGTG
GCAGGCCTGTTGGGCCTGGAGCTGTATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCC
TTTCGCCACAGCTACCTACATCTGGCTGAAAACTACCTCATCCGCTATATGCCTTTTGCC
CCAGCCATCCAGACGTTCCATCACCTGAAGTGGACCTCTTGGGACCGTCTGAAGGTACCA
GCTGGGCAGCCTGAGAGGACCCTGGAGTCGCTGCTGGCTCATCTTCAGGAGCAGCACGGG
TTGAGGGTGAGGATCCTGCTGCACGGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCT
GAAAAGCAGGCCCAGCACCTGCCCCTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGC
CAGGCACCTGCTCCTGGGCAGCGGGTGTTGGTGCTAGAGCTGAGCTGTGAGGGTGACGAC
GAGGACACTGCCTTCCCACCTCTGCACTATGAGCTG

Protein sequence predicted based on nucleotide sequence

MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLT
LHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQ
VVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAA
IQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGD
TTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKF
QHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPM
VAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIA
VFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQ
FLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALD
SFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCT
VRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLR
VRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTN
QDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASA
EFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRA
QSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFA
PAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSP
EKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 106
End on reference sequence 3144
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable