DNASU Plasmid | RASSF9 (Homo sapiens) in pVP16

Explanation of Terms

Gene:

Gene Symbol: RASSF9

Gene Name: Ras association (RalGDS/AF-6) domain family (N-terminal) member 9

Original Clone ID:

1136.1.44_GO.36994

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pVP16

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00396733

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1326nts

Open reading frame: 28 to 1353

Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCTCCCTTTGGA
AGAAACTTGCTAAAGACTCGGCATAAAAACAGATCTCCAACTAAAGACATGGATTCAGAA
GAGAAGGAAATTGTGGTTTGGGTTTGCCAAGAAGAGAAGCTTGTCTGTGGGCTGACTAAA
CGCACCACCTCTGCTGATGTCATCCAGGCTTTGCTTGAGGAACATGAGGCTACGTTTGGA
GAGAAACGATTTCTTCTGGGGAAGCCCAGTGATTACTGCATCATAGAGAAGTGGAGAGGC
TCCGAAAGGGTTCTTCCTCCACTAACTAGAATCCTGAAGCTTTGGAAAGCGTGGGGAGAT
GAGCAGCCCAATATGCAATTTGTTTTGGTTAAAGCAGATGCTTTTCTTCCAGTTCCTTTG
TGGCGGACAGCTGAAGCCAAATTAGTGCAAAACACAGAAAAATTGTGGGAGCTCAGCCCA
GCAAACTACATGAAGACTTTACCACCAGATAAACAAAAAAGAATAGTCAGGAAAACTTTC
CGGAAACTGGCTAAAATTAAGCAGGACACAGTTTCTCATGATCGAGATAATATGGAGACA
TTAGTTCATCTGATCATTTCCCAGGACCATACTATTCATCAGCAAGTCAAGAGAATGAAA
GAGCTGGATCTGGAAATTGAAAAGTGTGAAGCTAAGTTCCATCTTGATCGAGTAGAAAAT
GATGGAGAAAACTATGTTCAGGATGCATATTTAATGCCCAGTTTCAGTGAAGTTGAGCAA
AATCTAGACTTGCAGTATGAGGAAAACCAGACTCTGGAGGACCTGAGCGAAAGTGATGGA
ATTGAACAGCTGGAAGAACGACTGAAATATTACCGAATACTCATTGATAAGCTCTCTGCT
GAAATAGAAAAAGAGGTAAAAAGTGTTTGCATTGATATAAATGAAGATGCGGAAGGGGAA
GCTGCAAGTGAACTGGAAAGCTCTAATTTAGAGAGTGTTAAGTGTGATTTGGAGAAAAGC
ATGAAAGCTGGTTTGAAAATTCACTCTCATTTGAGTGGCATCCAGAAAGAGATTAAATAC
AGTGACTCATTGCTTCAGATGAAAGCAAAAGAATATGAACTCCTGGCCAAGGAATTCAAT
TCACTTCACATTAGCAACAAAGATGGGTGCCAGTTAAAGGAAAACAGAGCGAAGGAATCT
GAGGTTCCCAGTAGCAATGGGGAGATTCCTCCCTTTACTCAAAGAGTATTTAGCAATTAC
ACAAATGACACAGACTCGGACACTGGTATCAGTTCTAACCACAGTCAGGACTCCGAAACA
ACAGTAGGAGATGTGGTGCTGTTGTCAACATAGTAGGACC

Protein sequence predicted based on nucleotide sequence

ENLYFQSAPFGRNLLKTRHKNRSPTKDMDSEEKEIVVWVCQEEKLVCGLTKRTTSADVIQ
ALLEEHEATFGEKRFLLGKPSDYCIIEKWRGSERVLPPLTRILKLWKAWGDEQPNMQFVL
VKADAFLPVPLWRTAEAKLVQNTEKLWELSPANYMKTLPPDKQKRIVRKTFRKLAKIKQD
TVSHDRDNMETLVHLIISQDHTIHQQVKRMKELDLEIEKCEAKFHLDRVENDGENYVQDA
YLMPSFSEVEQNLDLQYEENQTLEDLSESDGIEQLEERLKYYRILIDKLSAEIEKEVKSV
CIDINEDAEGEAASELESSNLESVKCDLEKSMKAGLKIHSHLSGIQKEIKYSDSLLQMKA
KEYELLAKEFNSLHISNKDGCQLKENRAKESEVPSSNGEIPPFTQRVFSNYTNDTDSDTG
ISSNHSQDSETTVGDVVLLST*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

ProteinPurified: Tested_Not_Purified

Find experimental protocols in GO.36994

Recommended expression in:

Not Applicable

Vector Name:

pVP16

Synonyms:

Sequencing Primer:

Forward: MBP Forward

Reverse: pQE Reverse

Description:

Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.

Comments:

TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette

Size (bp):

7631

Parent Vector:

None

Properties:

Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15750721

Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:

Vector Sequence:

Sequence:

CCTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACCATGGGACATCACCATCATCACCATCACCATTCAAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGACGGTACCATCGATACGCGTTCGAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGATTACGTGCAGTCGATGATAAGCTGTCAAACATGAGAATTGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCGGAATTTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

6471

5789

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

2643

2852

promoter

T5 promoter

recombination site

AttR1

AttR1 recombination site

1316

1440

recombination site

AttR2

AttR2 recombination site

3020

2896

repressor protein gene

LacI

LacI coding region

4132

5211

selectable marker

CmR

chloramphenicol resistance gene (CmR) (Lost in with-insert form)

1549

2205

selectable marker

AmpR

ampicillin resistance gene

7228

6569

tag

MBP

N-terminal MBP

148

1246

tag

His

N-terminal 8xHis tag

128

145

Gene

Gene Symbol :

RASSF9

Symbol Nomenclature :

RASSF9

SYNONYM :

P-CIP1; PAMCI; PCIP1

Designation :

PAM COOH-terminal interactor protein 1|peptidylglycine alpha-amidating monooxygenase COOH-terminal interactor protein-1|ras association domain-containing protein 9

Full Nomenclature :

Ras association (RalGDS/AF-6) domain family (N-terminal) member 9

GENEID :

9182

GI :

21594849

GenBank Accession :

BC031589

HGNC :

15739

MIM :

610383

Vega :

OTTHUMG00000169831

Target GenBank:

BC031589

Reference Sequence Alignment

  

HsCD00396733      ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCTCCCTTTGGA
NM_005447.3       ---------------------------------------------ATGGCTCCCTTTGGA
                                                               :  ************

HsCD00396733      AGAAACTTGCTAAAGACTCGGCATAAAAACAGATCTCCAACTAAAGACATGGATTCAGAA
NM_005447.3       AGAAACTTGCTAAAGACTCGGCATAAAAACAGATCTCCAACTAAAGACATGGATTCAGAA
                  ************************************************************

HsCD00396733      GAGAAGGAAATTGTGGTTTGGGTTTGCCAAGAAGAGAAGCTTGTCTGTGGGCTGACTAAA
NM_005447.3       GAGAAGGAAATTGTGGTTTGGGTTTGCCAAGAAGAGAAGCTTGTCTGTGGGCTGACTAAA
                  ************************************************************

HsCD00396733      CGCACCACCTCTGCTGATGTCATCCAGGCTTTGCTTGAGGAACATGAGGCTACGTTTGGA
NM_005447.3       CGCACCACCTCTGCTGATGTCATCCAGGCTTTGCTTGAGGAACATGAGGCTACGTTTGGA
                  ************************************************************

HsCD00396733      GAGAAACGATTTCTTCTGGGGAAGCCCAGTGATTACTGCATCATAGAGAAGTGGAGAGGC
NM_005447.3       GAGAAACGATTTCTTCTGGGGAAGCCCAGTGATTACTGCATCATAGAGAAGTGGAGAGGC
                  ************************************************************

HsCD00396733      TCCGAAAGGGTTCTTCCTCCACTAACTAGAATCCTGAAGCTTTGGAAAGCGTGGGGAGAT
NM_005447.3       TCCGAAAGGGTTCTTCCTCCACTAACTAGAATCCTGAAGCTTTGGAAAGCGTGGGGAGAT
                  ************************************************************

HsCD00396733      GAGCAGCCCAATATGCAATTTGTTTTGGTTAAAGCAGATGCTTTTCTTCCAGTTCCTTTG
NM_005447.3       GAGCAGCCCAATATGCAATTTGTTTTGGTTAAAGCAGATGCTTTTCTTCCAGTTCCTTTG
                  ************************************************************

HsCD00396733      TGGCGGACAGCTGAAGCCAAATTAGTGCAAAACACAGAAAAATTGTGGGAGCTCAGCCCA
NM_005447.3       TGGCGGACAGCTGAAGCCAAATTAGTGCAAAACACAGAAAAATTGTGGGAGCTCAGCCCA
                  ************************************************************

HsCD00396733      GCAAACTACATGAAGACTTTACCACCAGATAAACAAAAAAGAATAGTCAGGAAAACTTTC
NM_005447.3       GCAAACTACATGAAGACTTTACCACCAGATAAACAAAAAAGAATAGTCAGGAAAACTTTC
                  ************************************************************

HsCD00396733      CGGAAACTGGCTAAAATTAAGCAGGACACAGTTTCTCATGATCGAGATAATATGGAGACA
NM_005447.3       CGGAAACTGGCTAAAATTAAGCAGGACACAGTTTCTCATGATCGAGATAATATGGAGACA
                  ************************************************************

HsCD00396733      TTAGTTCATCTGATCATTTCCCAGGACCATACTATTCATCAGCAAGTCAAGAGAATGAAA
NM_005447.3       TTAGTTCATCTGATCATTTCCCAGGACCATACTATTCATCAGCAAGTCAAGAGAATGAAA
                  ************************************************************

HsCD00396733      GAGCTGGATCTGGAAATTGAAAAGTGTGAAGCTAAGTTCCATCTTGATCGAGTAGAAAAT
NM_005447.3       GAGCTGGATCTGGAAATTGAAAAGTGTGAAGCTAAGTTCCATCTTGATCGAGTAGAAAAT
                  ************************************************************

HsCD00396733      GATGGAGAAAACTATGTTCAGGATGCATATTTAATGCCCAGTTTCAGTGAAGTTGAGCAA
NM_005447.3       GATGGAGAAAACTATGTTCAGGATGCATATTTAATGCCCAGTTTCAGTGAAGTTGAGCAA
                  ************************************************************

HsCD00396733      AATCTAGACTTGCAGTATGAGGAAAACCAGACTCTGGAGGACCTGAGCGAAAGTGATGGA
NM_005447.3       AATCTAGACTTGCAGTATGAGGAAAACCAGACTCTGGAGGACCTGAGCGAAAGTGATGGA
                  ************************************************************

HsCD00396733      ATTGAACAGCTGGAAGAACGACTGAAATATTACCGAATACTCATTGATAAGCTCTCTGCT
NM_005447.3       ATTGAACAGCTGGAAGAACGACTGAAATATTACCGAATACTCATTGATAAGCTCTCTGCT
                  ************************************************************

HsCD00396733      GAAATAGAAAAAGAGGTAAAAAGTGTTTGCATTGATATAAATGAAGATGCGGAAGGGGAA
NM_005447.3       GAAATAGAAAAAGAGGTAAAAAGTGTTTGCATTGATATAAATGAAGATGCGGAAGGGGAA
                  ************************************************************

HsCD00396733      GCTGCAAGTGAACTGGAAAGCTCTAATTTAGAGAGTGTTAAGTGTGATTTGGAGAAAAGC
NM_005447.3       GCTGCAAGTGAACTGGAAAGCTCTAATTTAGAGAGTGTTAAGTGTGATTTGGAGAAAAGC
                  ************************************************************

HsCD00396733      ATGAAAGCTGGTTTGAAAATTCACTCTCATTTGAGTGGCATCCAGAAAGAGATTAAATAC
NM_005447.3       ATGAAAGCTGGTTTGAAAATTCACTCTCATTTGAGTGGCATCCAGAAAGAGATTAAATAC
                  ************************************************************

HsCD00396733      AGTGACTCATTGCTTCAGATGAAAGCAAAAGAATATGAACTCCTGGCCAAGGAATTCAAT
NM_005447.3       AGTGACTCATTGCTTCAGATGAAAGCAAAAGAATATGAACTCCTGGCCAAGGAATTCAAT
                  ************************************************************

HsCD00396733      TCACTTCACATTAGCAACAAAGATGGGTGCCAGTTAAAGGAAAACAGAGCGAAGGAATCT
NM_005447.3       TCACTTCACATTAGCAACAAAGATGGGTGCCAGTTAAAGGAAAACAGAGCGAAGGAATCT
                  ************************************************************

HsCD00396733      GAGGTTCCCAGTAGCAATGGGGAGATTCCTCCCTTTACTCAAAGAGTATTTAGCAATTAC
NM_005447.3       GAGGTTCCCAGTAGCAATGGGGAGATTCCTCCCTTTACTCAAAGAGTATTTAGCAATTAC
                  ************************************************************

HsCD00396733      ACAAATGACACAGACTCGGACACTGGTATCAGTTCTAACCACAGTCAGGACTCCGAAACA
NM_005447.3       ACAAATGACACAGACTCGGACACTGGTATCAGTTCTAACCACAGTCAGGACTCCGAAACA
                  ************************************************************

HsCD00396733      ACAGTAGGAGATGTGGTGCTGTTGTCAACATAGTAGGACC
NM_005447.3       ACAGTAGGAGATGTGGTGCTGTTGTCAACATAG-------
                  *********************************