DNASU Plasmid | PSMD12 (Homo sapiens) in pVP16

Explanation of Terms

Gene:

Gene Symbol: PSMD12

Gene Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 12

Original Clone ID:

1136.1.58_GO.36854

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pVP16

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00396745

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1389nts

Open reading frame: 51 to 1439

Reference Sequence Alignment

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCGGACGGCGGCTCGGAGCGGGCTGACGGGCGCATCGTCAAGATGGAGG
TGGACTACAGCGCCACGGTGGATCAGCGCCTACCCGAGTGTGCGAAGCTAGCCAAGGAAG
GAAGACTTCAAGAAGTCATTGAAACCCTTCTCTCTCTGGAAAAGCAGACTCGTACTGCTT
CCGATATGGTATCGACATCCCGTATCTTAGTTGCAGTAGTGAAGATGTGCTATGAGGCTA
AAGAATGGGATTTACTTAATGAAAATATTATGCTTTTGTCCAAAAGGCGGAGTCAGTTAA
AACAAGCTGTTGCCAAAATGGTTCAACAGTGCTGTACTTATGTTGAGGAAATCACAGACC
TTCCTATCAAACTTCGATTAATTGATACTCTACGAATGGTTACCGAAGGCAAGATTTATG
TTGAAATTGAGCGTGCGCGACTGACTAAAACATTAGCAACTATAAAAGAACAAAATGGTG
ATGTGAAAGAGGCAGCCTCCATTTTACAGGAGTTACAGGTGGAAACCTACGGGTCAATGG
AAAAGAAAGAGCGAGTGGAATTTATTTTGGAGCAAATGAGGCTCTGCCTAGCTGTGAAGG
ATTACATTCGAACACAAATCATCAGCAAGAAAATTAACACCAAATTTTTCCAGGAAGAAA
ATACAGAGAAATTAAAGTTGAAGTACTATAATTTAATGATTCAGCTGGATCAACATGAGG
GATCCTATTTGTCTATTTGTAAGCACTACAGAGCAATATATGATACTCCCTGTATACAGG
CAGAAAGTGAAAAATGGCAGCAGGCTCTGAAGAGTGTTGTACTCTATGTTATCCTGGCTC
CTTTTGACAATGAACAGTCAGATTTGGTTCACCGAATAAGTGGTGACAAGAAGTTAGAAG
AAATTCCCAAATACAAGGATCTTTTAAAGCTTTTTACCACAATGGAGTTGATGCGTTGGT
CCACACTTGTTGAGGACTATGGAATGGAATTAAGAAAAGGTTCCCTTGAGAGTCCTGCAA
CGGATGTTTTTGGTTCTACAGAGGAAGGTGAAAAAAGGTGGAAAGACTTGAAGAACAGAG
TTGTTGAACATAATATTAGAATAATGGCCAAGTATTATACTCGGATAACAATGAAAAGGA
TGGCACAGCTTCTGGATCTATCTGTTGATGAGTCCGAAGCCTTTCTCTCAAATCTAGTAG
TTAACAAGACCATCTTTGCTAAAGTAGACAGATTAGCAGGAATTATCAACTTCCAGAGAC
CCAAGGATCCAAATAATTTATTAAATGACTGGTCTCAGAAACTGAACTCATTAATGTCTC
TGGTTAACAAAACTACGCATCTCATAGCCAAAGAGGAGATGATACATAATCTACAATAAT
AGGACCCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTC

Protein sequence predicted based on nucleotide sequence

ENLYFQSADGGSERADGRIVKMEVDYSATVDQRLPECAKLAKEGRLQEVIETLLSLEKQT
RTASDMVSTSRILVAVVKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEE
ITDLPIKLRLIDTLRMVTEGKIYVEIERARLTKTLATIKEQNGDVKEAASILQELQVETY
GSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKINTKFFQEENTEKLKLKYYNLMIQLD
QHEGSYLSICKHYRAIYDTPCIQAESEKWQQALKSVVLYVILAPFDNEQSDLVHRISGDK
KLEEIPKYKDLLKLFTTMELMRWSTLVEDYGMELRKGSLESPATDVFGSTEEGEKRWKDL
KNRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGIIN
FQRPKDPNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

SolubleProtein: Not_Applicable

ProteinPurified: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in GO.36854

Recommended expression in:

Not Applicable

Vector Name:

pVP16

Synonyms:

Sequencing Primer:

Forward: MBP Forward

Reverse: pQE Reverse

Description:

Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.

Comments:

TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette

Size (bp):

7631

Parent Vector:

None

Properties:

Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15750721

Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:

Vector Sequence:

Sequence:

CCTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACCATGGGACATCACCATCATCACCATCACCATTCAAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGACGGTACCATCGATACGCGTTCGAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGATTACGTGCAGTCGATGATAAGCTGTCAAACATGAGAATTGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCGGAATTTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

6471

5789

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

2643

2852

promoter

T5 promoter

recombination site

AttR1

AttR1 recombination site

1316

1440

recombination site

AttR2

AttR2 recombination site

3020

2896

repressor protein gene

LacI

LacI coding region

4132

5211

selectable marker

CmR

chloramphenicol resistance gene (CmR) (Lost in with-insert form)

1549

2205

selectable marker

AmpR

ampicillin resistance gene

7228

6569

tag

MBP

N-terminal MBP

148

1246

tag

His

N-terminal 8xHis tag

128

145

Gene

Gene Symbol :

PSMD12

Symbol Nomenclature :

PSMD12

SYNONYM :

Rpn5; p55

Designation :

26S proteasome non-ATPase regulatory subunit 12|26S proteasome regulatory subunit RPN5|26S proteasome regulatory subunit p55

Full Nomenclature :

proteasome (prosome, macropain) 26S subunit, non-ATPase, 12

GENEID :

5718

GI :

17512153

GenBank Accession :

BC019062

HGNC :

9557

MIM :

604450

Target GenBank:

BC019062

Reference Sequence Alignment

  

HsCD00396745      GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
NM_002816.3       ------------------------------------------------------------
                                                                              

HsCD00396745      ACTTCCAGTCCGCGGACGGCGGCTCGGAGCGGGCTGACGGGCGCATCGTCAAGATGGAGG
NM_002816.3       --------ATGGCGGACGGCGGCTCGGAGCGGGCTGACGGGCGCATCGTCAAGATGGAGG
                          :  *************************************************

HsCD00396745      TGGACTACAGCGCCACGGTGGATCAGCGCCTACCCGAGTGTGCGAAGCTAGCCAAGGAAG
NM_002816.3       TGGACTACAGCGCCACGGTGGATCAGCGCCTACCCGAGTGTGCGAAGCTAGCCAAGGAAG
                  ************************************************************

HsCD00396745      GAAGACTTCAAGAAGTCATTGAAACCCTTCTCTCTCTGGAAAAGCAGACTCGTACTGCTT
NM_002816.3       GAAGACTTCAAGAAGTCATTGAAACCCTTCTCTCTCTGGAAAAGCAGACTCGTACTGCTT
                  ************************************************************

HsCD00396745      CCGATATGGTATCGACATCCCGTATCTTAGTTGCAGTAGTGAAGATGTGCTATGAGGCTA
NM_002816.3       CCGATATGGTATCGACATCCCGTATCTTAGTTGCAGTAGTGAAGATGTGCTATGAGGCTA
                  ************************************************************

HsCD00396745      AAGAATGGGATTTACTTAATGAAAATATTATGCTTTTGTCCAAAAGGCGGAGTCAGTTAA
NM_002816.3       AAGAATGGGATTTACTTAATGAAAATATTATGCTTTTGTCCAAAAGGCGGAGTCAGTTAA
                  ************************************************************

HsCD00396745      AACAAGCTGTTGCCAAAATGGTTCAACAGTGCTGTACTTATGTTGAGGAAATCACAGACC
NM_002816.3       AACAAGCTGTTGCCAAAATGGTTCAACAGTGCTGTACTTATGTTGAGGAAATCACAGACC
                  ************************************************************

HsCD00396745      TTCCTATCAAACTTCGATTAATTGATACTCTACGAATGGTTACCGAAGGCAAGATTTATG
NM_002816.3       TTCCTATCAAACTTCGATTAATTGATACTCTACGAATGGTTACCGAAGGCAAGATTTATG
                  ************************************************************

HsCD00396745      TTGAAATTGAGCGTGCGCGACTGACTAAAACATTAGCAACTATAAAAGAACAAAATGGTG
NM_002816.3       TTGAAATTGAGCGTGCGCGACTGACTAAAACATTAGCAACTATAAAAGAACAAAATGGTG
                  ************************************************************

HsCD00396745      ATGTGAAAGAGGCAGCCTCCATTTTACAGGAGTTACAGGTGGAAACCTACGGGTCAATGG
NM_002816.3       ATGTGAAAGAGGCAGCCTCCATTTTACAGGAGTTACAGGTGGAAACCTACGGGTCAATGG
                  ************************************************************

HsCD00396745      AAAAGAAAGAGCGAGTGGAATTTATTTTGGAGCAAATGAGGCTCTGCCTAGCTGTGAAGG
NM_002816.3       AAAAGAAAGAGCGAGTGGAATTTATTTTGGAGCAAATGAGGCTCTGCCTAGCTGTGAAGG
                  ************************************************************

HsCD00396745      ATTACATTCGAACACAAATCATCAGCAAGAAAATTAACACCAAATTTTTCCAGGAAGAAA
NM_002816.3       ATTACATTCGAACACAAATCATCAGCAAGAAAATTAACACCAAATTTTTCCAGGAAGAAA
                  ************************************************************

HsCD00396745      ATACAGAGAAATTAAAGTTGAAGTACTATAATTTAATGATTCAGCTGGATCAACATGAGG
NM_002816.3       ATACAGAGAAATTAAAGTTGAAGTACTATAATTTAATGATTCAGCTGGATCAACATGAGG
                  ************************************************************

HsCD00396745      GATCCTATTTGTCTATTTGTAAGCACTACAGAGCAATATATGATACTCCCTGTATACAGG
NM_002816.3       GATCCTATTTGTCTATTTGTAAGCACTACAGAGCAATATATGATACTCCCTGTATACAGG
                  ************************************************************

HsCD00396745      CAGAAAGTGAAAAATGGCAGCAGGCTCTGAAGAGTGTTGTACTCTATGTTATCCTGGCTC
NM_002816.3       CAGAAAGTGAAAAATGGCAGCAGGCTCTGAAGAGTGTTGTACTCTATGTTATCCTGGCTC
                  ************************************************************

HsCD00396745      CTTTTGACAATGAACAGTCAGATTTGGTTCACCGAATAAGTGGTGACAAGAAGTTAGAAG
NM_002816.3       CTTTTGACAATGAACAGTCAGATTTGGTTCACCGAATAAGTGGTGACAAGAAGTTAGAAG
                  ************************************************************

HsCD00396745      AAATTCCCAAATACAAGGATCTTTTAAAGCTTTTTACCACAATGGAGTTGATGCGTTGGT
NM_002816.3       AAATTCCCAAATACAAGGATCTTTTAAAGCTTTTTACCACAATGGAGTTGATGCGTTGGT
                  ************************************************************

HsCD00396745      CCACACTTGTTGAGGACTATGGAATGGAATTAAGAAAAGGTTCCCTTGAGAGTCCTGCAA
NM_002816.3       CCACACTTGTTGAGGACTATGGAATGGAATTAAGAAAAGGTTCCCTTGAGAGTCCTGCAA
                  ************************************************************

HsCD00396745      CGGATGTTTTTGGTTCTACAGAGGAAGGTGAAAAAAGGTGGAAAGACTTGAAGAACAGAG
NM_002816.3       CGGATGTTTTTGGTTCTACAGAGGAAGGTGAAAAAAGGTGGAAAGACTTGAAGAACAGAG
                  ************************************************************

HsCD00396745      TTGTTGAACATAATATTAGAATAATGGCCAAGTATTATACTCGGATAACAATGAAAAGGA
NM_002816.3       TTGTTGAACATAATATTAGAATAATGGCCAAGTATTATACTCGGATAACAATGAAAAGGA
                  ************************************************************

HsCD00396745      TGGCACAGCTTCTGGATCTATCTGTTGATGAGTCCGAAGCCTTTCTCTCAAATCTAGTAG
NM_002816.3       TGGCACAGCTTCTGGATCTATCTGTTGATGAGTCCGAAGCCTTTCTCTCAAATCTAGTAG
                  ************************************************************

HsCD00396745      TTAACAAGACCATCTTTGCTAAAGTAGACAGATTAGCAGGAATTATCAACTTCCAGAGAC
NM_002816.3       TTAACAAGACCATCTTTGCTAAAGTAGACAGATTAGCAGGAATTATCAACTTCCAGAGAC
                  ************************************************************

HsCD00396745      CCAAGGATCCAAATAATTTATTAAATGACTGGTCTCAGAAACTGAACTCATTAATGTCTC
NM_002816.3       CCAAGGATCCAAATAATTTATTAAATGACTGGTCTCAGAAACTGAACTCATTAATGTCTC
                  ************************************************************

HsCD00396745      TGGTTAACAAAACTACGCATCTCATAGCCAAAGAGGAGATGATACATAATCTACAATAAT
NM_002816.3       TGGTTAACAAAACTACGCATCTCATAGCCAAAGAGGAGATGATACATAATCTACAATAA-
                  *********************************************************** 

HsCD00396745      AGGACCCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTC
NM_002816.3       --------------------------------------------------

Pathways

Panther :

Cell cycle

Reactome :

Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

Activation of NF-kappaB in B cells

Reactome :

Adaptive Immune System

Reactome :

Antigen processing-Cross presentation

Reactome :

Antigen processing: Ubiquitination & Proteasome degradation

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

APC/C:Cdc20 mediated degradation of Securin

Reactome :

APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1

Reactome :

Apoptosis

Reactome :

Assembly of the pre-replicative complex

Reactome :

Asymmetric localization of PCP proteins

Reactome :

AUF1 (hnRNP D0) destabilizes mRNA

Reactome :

Autodegradation of Cdh1 by Cdh1:APC/C

Reactome :

Autodegradation of the E3 ubiquitin ligase COP1

Reactome :

beta-catenin independent WNT signaling

Reactome :

Cdc20:Phospho-APC/C mediated degradation of Cyclin A

Reactome :

CDK-mediated phosphorylation and removal of Cdc6

Reactome :

CDT1 association with the CDC6:ORC:origin complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Class I MHC mediated antigen processing & presentation

Reactome :

Cross-presentation of soluble exogenous antigens (endosomes)

Reactome :

Cyclin A:Cdk2-associated events at S phase entry

Reactome :

Cyclin E associated events during G1/S transition

Reactome :

degradation of AXIN

Reactome :

degradation of DVL

Reactome :

Disease

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

Downstream signaling events of B Cell Receptor (BCR)

Reactome :

ER-Phagosome pathway

Reactome :

G1/S DNA Damage Checkpoints

Reactome :

G1/S Transition

Reactome :

Gene Expression

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

M Phase

Reactome :

M/G1 Transition

Reactome :

Metabolism

Reactome :

Metabolism of amino acids and derivatives

Reactome :

Metabolism of mRNA

Reactome :

Metabolism of RNA

Reactome :

Mitotic Anaphase

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Orc1 removal from chromatin

Reactome :

p53-Dependent G1 DNA Damage Response

Reactome :

p53-Dependent G1/S DNA damage checkpoint

Reactome :

p53-Independent DNA Damage Response

Reactome :

p53-Independent G1/S DNA damage checkpoint

Reactome :

PCP/CE pathway

Reactome :

Regulation of activated PAK-2p34 by proteasome mediated degradation

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of Apoptosis

Reactome :

Regulation of DNA replication

Reactome :

Regulation of mitotic cell cycle

Reactome :

Regulation of mRNA stability by proteins that bind AU-rich elements

Reactome :

Regulation of ornithine decarboxylase (ODC)

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

SCF(Skp2)-mediated degradation of p27/p21

Reactome :

SCF-beta-TrCP mediated degradation of Emi1

Reactome :

Separation of Sister Chromatids

Reactome :

Signal Transduction

Reactome :

Signaling by the B Cell Receptor (BCR)

Reactome :

Signaling by Wnt

Reactome :

Stabilization of p53

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

Reactome :

TCF dependent signaling in response to WNT

Reactome :

Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Reactome :

Ubiquitin-dependent degradation of Cyclin D

Reactome :

Ubiquitin-dependent degradation of Cyclin D1

Reactome :

Vif-mediated degradation of APOBEC3G

Reactome :

Vpu mediated degradation of CD4

WikiPathway :

Proteasome Degradation

Protein

SMART domain :

PINT : motif in proteasome subunits, Int-6, Nip-1 and TRIP-15

Structural Annotation Wiki :

TOPSAN

UniProt :

A6NP15

UniProt :

O00232

HPRD :

05120

Clone

Cloning Information :

474559

HIP Master Clone ID :

396065

Original Clone ID :

1136.1.58_GO.36854

Experimental

TargetTrack - Experimental data :

GO.36854

Organism

Species Specific ID:

5718

Genome

Chromosome :

Map Location :

17q24.2

Reagents

Labome :

PSMD12-antibody