DNASU Plasmid | TAB1 (Homo sapiens) in pVP16

Explanation of Terms

Gene:

Gene Symbol: TAB1

Gene Name: TGF-beta activated kinase 1/MAP3K7 binding protein 1

Original Clone ID:

1136.1.71_GO.37146

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pVP16

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00396751

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1533nts

Open reading frame: 51 to 1583

Reference Sequence Alignment

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCGGCGCAGAGGAGGAGCTTGCTGCAGAGTGAGCAGCAGCCAAGCTGGA
CAGATGACCTGCCTCTCTGCCACCTCTCTGGGGTTGGCTCAGCCTCCAACCGCAGCTACT
CTGCTGATGGCAAGGGCACTGAGAGCCACCCGCCAGAGGACAGCTGGCTCAAGTTCAGGA
GTGAGAACAACTGCTTCCTGTATGGGGTCTTCAACGGCTATGATGGCAACCGAGTGACCA
ACTTCGTGGCCCAGCGGCTGTCCGCAGAGCTCCTGCTGGGCCAGCTGAATGCCGAGCACG
CCGAGGCCGATGTGCGGCGTGTGCTGCTGCAGGCCTTCGATGTGGTGGAGAGGAGCTTCC
TGGAGTCCATTGACGACGCCTTGGCTGAGAAGGCAAGCCTCCAGTCGCAATTGCCAGAGG
GAGTCCCTCAGCACCAGCTGCCTCCTCAGTATCAGAAGATCCTTGAGAGACTCAAGACGT
TAGAGAGGGAAATTTCGGGAGGGGCCATGGCCGTTGTGGCGGTCCTTCTCAACAACAAGC
TCTACGTCGCCAATGTCGGTACAAACCGTGCACTTTTATGCAAATCGACAGTGGATGGGT
TGCAGGTGACACAGCTGAACGTGGACCACACCACAGAGAACGAGGATGAGCTCTTCCGTC
TTTCGCAGCTGGGCTTGGATGCTGGAAAGATCAAGCAGGTGGGGATCATCTGTGGGCAGG
AGAGCACCCGGCGGATCGGGGATTACAAGGTTAAATATGGCTACACGGACATTGACCTTC
TCAGCGCTGCCAAGTCCAAACCAATCATCGCAGAGCCAGAAATCCATGGGGCACAGCCGC
TGGATGGGGTGACGGGCTTCTTGGTGCTGATGTCGGAGGGGTTGTACAAGGCCCTAGAGG
CAGCCCATGGGCCTGGGCAGGCCAACCAGGAGATTGCTGCGATGATTGACACTGAGTTTG
CCAAGCAGACCTCCCTGGACGCAGTGGCCCAGGCCGTCGTGGACCGGGTGAAGCGCATCC
ACAGCGACACCTTCGCCAGTGGTGGGGAGCGTGCCAGGTTCTGCCCCCGGCACGAGGACA
TGACCCTGCTAGTGAGGAACTTTGGCTACCCGCTGGGCGAAATGAGCCAGCCCACACCGA
GCCCAGCCCCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTGTGCCATACTCCAGCGCCC
AGAGCACCAGCAAGACCAGCGTGACCCTCTCCCTTGTCATGCCCTCCCAGGGCCAGATGG
TCAACGGGGCTCACAGTGCTTCCACCCTGGACGAAGCCACCCCCACCCTCACCAACCAAA
GCCCGACCTTAACCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTG
ACGGAGGCCTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGACGGTCGTG
TTGAGCCCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGAGCGTGGACCATGGCGAGC
AGAGCGTGGTGACAGCACCGTAGTAGGACCCAGCTTTCTTGTACAAAGTGGTGATGGCGC
GCCTGTAGGACGTC

Protein sequence predicted based on nucleotide sequence

ENLYFQSAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDSWL
KFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNAEHAEADVRRVLLQAFDVVE
RSFLESIDDALAEKASLQSQLPEGVPQHQLPPQYQKILERLKTLEREISGGAMAVVAVLL
NNKLYVANVGTNRALLCKSTVDGLQVTQLNVDHTTENEDELFRLSQLGLDAGKIKQVGII
CGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDGVTGFLVLMSEGLYK
ALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERARFCPR
HEDMTLLVRNFGYPLGEMSQPTPSPAPAAGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQ
GQMVNGAHSASTLDEATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGE
DGRVEPYVDFAEFYRLWSVDHGEQSVVTAP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in GO.37146

Recommended expression in:

Not Applicable

Vector Name:

pVP16

Synonyms:

Sequencing Primer:

Forward: MBP Forward

Reverse: pQE Reverse

Description:

Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.

Comments:

TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette

Size (bp):

7631

Parent Vector:

None

Properties:

Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15750721

Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:

Vector Sequence:

Sequence:

CCTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACCATGGGACATCACCATCATCACCATCACCATTCAAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGACGGTACCATCGATACGCGTTCGAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGATTACGTGCAGTCGATGATAAGCTGTCAAACATGAGAATTGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCGGAATTTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

6471

5789

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

2643

2852

promoter

T5 promoter

recombination site

AttR1

AttR1 recombination site

1316

1440

recombination site

AttR2

AttR2 recombination site

3020

2896

repressor protein gene

LacI

LacI coding region

4132

5211

selectable marker

CmR

chloramphenicol resistance gene (CmR) (Lost in with-insert form)

1549

2205

selectable marker

AmpR

ampicillin resistance gene

7228

6569

tag

MBP

N-terminal MBP

148

1246

tag

His

N-terminal 8xHis tag

128

145

Gene

Gene Symbol :

TAB1

Symbol Nomenclature :

TAB1

SYNONYM :

3'-Tab1; MAP3K7IP1

Designation :

TAK1-binding protein 1|TGF-beta-activated kinase 1 and MAP3K7-binding protein 1|mitogen-activated protein kinase kinase kinase 7-interacting protein 1|transforming growth factor beta-activated kinase-binding protein 1

Full Nomenclature :

TGF-beta activated kinase 1/MAP3K7 binding protein 1

GENEID :

10454

Locus Tag :

RP3-407F17.2

GI :

29791645

GenBank Accession :

BC050554

HGNC :

18157

MIM :

602615

Vega :

OTTHUMG00000151102

Target GenBank:

BC050554

Reference Sequence Alignment

  

HsCD00396751      GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
NM_006116.2       ------------------------------------------------------------
                                                                              

HsCD00396751      ACTTCCAGTCCGCGGCGCAGAGGAGGAGCTTGCTGCAGAGTGAGCAGCAGCCAAGCTGGA
NM_006116.2       --------ATGGCGGCGCAGAGGAGGAGCTTGCTGCAGAGTGAGCAGCAGCCAAGCTGGA
                          :  *************************************************

HsCD00396751      CAGATGACCTGCCTCTCTGCCACCTCTCTGGGGTTGGCTCAGCCTCCAACCGCAGCTACT
NM_006116.2       CAGATGACCTGCCTCTCTGCCACCTCTCTGGGGTTGGCTCAGCCTCCAACCGCAGCTACT
                  ************************************************************

HsCD00396751      CTGCTGATGGCAAGGGCACTGAGAGCCACCCGCCAGAGGACAGCTGGCTCAAGTTCAGGA
NM_006116.2       CTGCTGATGGCAAGGGCACTGAGAGCCACCCGCCAGAGGACAGCTGGCTCAAGTTCAGGA
                  ************************************************************

HsCD00396751      GTGAGAACAACTGCTTCCTGTATGGGGTCTTCAACGGCTATGATGGCAACCGAGTGACCA
NM_006116.2       GTGAGAACAACTGCTTCCTGTATGGGGTCTTCAACGGCTATGATGGCAACCGAGTGACCA
                  ************************************************************

HsCD00396751      ACTTCGTGGCCCAGCGGCTGTCCGCAGAGCTCCTGCTGGGCCAGCTGAATGCCGAGCACG
NM_006116.2       ACTTCGTGGCCCAGCGGCTGTCCGCAGAGCTCCTGCTGGGCCAGCTGAATGCCGAGCACG
                  ************************************************************

HsCD00396751      CCGAGGCCGATGTGCGGCGTGTGCTGCTGCAGGCCTTCGATGTGGTGGAGAGGAGCTTCC
NM_006116.2       CCGAGGCCGATGTGCGGCGTGTGCTGCTGCAGGCCTTCGATGTGGTGGAGAGGAGCTTCC
                  ************************************************************

HsCD00396751      TGGAGTCCATTGACGACGCCTTGGCTGAGAAGGCAAGCCTCCAGTCGCAATTGCCAGAGG
NM_006116.2       TGGAGTCCATTGACGACGCCTTGGCTGAGAAGGCAAGCCTCCAGTCGCAATTGCCAGAGG
                  ************************************************************

HsCD00396751      GAGTCCCTCAGCACCAGCTGCCTCCTCAGTATCAGAAGATCCTTGAGAGACTCAAGACGT
NM_006116.2       GAGTCCCTCAGCACCAGCTGCCTCCTCAGTATCAGAAGATCCTTGAGAGACTCAAGACGT
                  ************************************************************

HsCD00396751      TAGAGAGGGAAATTTCGGGAGGGGCCATGGCCGTTGTGGCGGTCCTTCTCAACAACAAGC
NM_006116.2       TAGAGAGGGAAATTTCGGGAGGGGCCATGGCCGTTGTGGCGGTCCTTCTCAACAACAAGC
                  ************************************************************

HsCD00396751      TCTACGTCGCCAATGTCGGTACAAACCGTGCACTTTTATGCAAATCGACAGTGGATGGGT
NM_006116.2       TCTACGTCGCCAATGTCGGTACAAACCGTGCACTTTTATGCAAATCGACAGTGGATGGGT
                  ************************************************************

HsCD00396751      TGCAGGTGACACAGCTGAACGTGGACCACACCACAGAGAACGAGGATGAGCTCTTCCGTC
NM_006116.2       TGCAGGTGACACAGCTGAACGTGGACCACACCACAGAGAACGAGGATGAGCTCTTCCGTC
                  ************************************************************

HsCD00396751      TTTCGCAGCTGGGCTTGGATGCTGGAAAGATCAAGCAGGTGGGGATCATCTGTGGGCAGG
NM_006116.2       TTTCGCAGCTGGGCTTGGATGCTGGAAAGATCAAGCAGGTGGGGATCATCTGTGGGCAGG
                  ************************************************************

HsCD00396751      AGAGCACCCGGCGGATCGGGGATTACAAGGTTAAATATGGCTACACGGACATTGACCTTC
NM_006116.2       AGAGCACCCGGCGGATCGGGGATTACAAGGTTAAATATGGCTACACGGACATTGACCTTC
                  ************************************************************

HsCD00396751      TCAGCGCTGCCAAGTCCAAACCAATCATCGCAGAGCCAGAAATCCATGGGGCACAGCCGC
NM_006116.2       TCAGCGCTGCCAAGTCCAAACCAATCATCGCAGAGCCAGAAATCCATGGGGCACAGCCGC
                  ************************************************************

HsCD00396751      TGGATGGGGTGACGGGCTTCTTGGTGCTGATGTCGGAGGGGTTGTACAAGGCCCTAGAGG
NM_006116.2       TGGATGGGGTGACGGGCTTCTTGGTGCTGATGTCGGAGGGGTTGTACAAGGCCCTAGAGG
                  ************************************************************

HsCD00396751      CAGCCCATGGGCCTGGGCAGGCCAACCAGGAGATTGCTGCGATGATTGACACTGAGTTTG
NM_006116.2       CAGCCCATGGGCCTGGGCAGGCCAACCAGGAGATTGCTGCGATGATTGACACTGAGTTTG
                  ************************************************************

HsCD00396751      CCAAGCAGACCTCCCTGGACGCAGTGGCCCAGGCCGTCGTGGACCGGGTGAAGCGCATCC
NM_006116.2       CCAAGCAGACCTCCCTGGACGCAGTGGCCCAGGCCGTCGTGGACCGGGTGAAGCGCATCC
                  ************************************************************

HsCD00396751      ACAGCGACACCTTCGCCAGTGGTGGGGAGCGTGCCAGGTTCTGCCCCCGGCACGAGGACA
NM_006116.2       ACAGCGACACCTTCGCCAGTGGTGGGGAGCGTGCCAGGTTCTGCCCCCGGCACGAGGACA
                  ************************************************************

HsCD00396751      TGACCCTGCTAGTGAGGAACTTTGGCTACCCGCTGGGCGAAATGAGCCAGCCCACACCGA
NM_006116.2       TGACCCTGCTAGTGAGGAACTTTGGCTACCCGCTGGGCGAAATGAGCCAGCCCACACCGA
                  ************************************************************

HsCD00396751      GCCCAGCCCCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTGTGCCATACTCCAGCGCCC
NM_006116.2       GCCCAGCCCCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTGTGCCATACTCCAGCGCCC
                  ************************************************************

HsCD00396751      AGAGCACCAGCAAGACCAGCGTGACCCTCTCCCTTGTCATGCCCTCCCAGGGCCAGATGG
NM_006116.2       AGAGCACCAGCAAGACCAGCGTGACCCTCTCCCTTGTCATGCCCTCCCAGGGCCAGATGG
                  ************************************************************

HsCD00396751      TCAACGGGGCTCACAGTGCTTCCACCCTGGACGAAGCCACCCCCACCCTCACCAACCAAA
NM_006116.2       TCAACGGGGCTCACAGTGCTTCCACCCTGGACGAAGCCACCCCCACCCTCACCAACCAAA
                  ************************************************************

HsCD00396751      GCCCGACCTTAACCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTG
NM_006116.2       GCCCGACCTTAACCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTG
                  ************************************************************

HsCD00396751      ACGGAGGCCTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGACGGTCGTG
NM_006116.2       ACGGAGGCCTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGACGGTCGTG
                  ************************************************************

HsCD00396751      TTGAGCCCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGAGCGTGGACCATGGCGAGC
NM_006116.2       TTGAGCCCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGAGCGTGGACCATGGCGAGC
                  ************************************************************

HsCD00396751      AGAGCGTGGTGACAGCACCGTAGTAGGACCCAGCTTTCTTGTACAAAGTGGTGATGGCGC
NM_006116.2       AGAGCGTGGTGACAGCACCGTAG-------------------------------------
                  ***********************                                     

HsCD00396751      GCCTGTAGGACGTC
NM_006116.2       --------------

Pathways

NCI :

BMP receptor signaling

NCI :

C-MYB transcription factor network

NCI :

IL1-mediated signaling events

NCI :

Noncanonical Wnt signaling pathway

NCI :

p38 MAPK signaling pathway

NCI :

Presenilin action in Notch and Wnt signaling

NCI :

Regulation of p38-alpha and p38-beta

NCI :

TGF-beta receptor signaling

NCI :

TNF receptor signaling pathway

Panther :

p38 MAPK pathway

Panther :

TGF-beta signaling pathway

Panther :

Toll receptor signaling pathway

Reactome :

activated TAK1 mediates p38 MAPK activation

Reactome :

Activated TLR4 signalling

Reactome :

Cytokine Signaling in Immune system

Reactome :

Fc epsilon receptor (FCERI) signaling

Reactome :

FCERI mediated NF-kB activation

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Interleukin-1 signaling

Reactome :

IRAK2 mediated activation of TAK1 complex

Reactome :

IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation

Reactome :

JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1

Reactome :

MAP kinase activation in TLR cascade

Reactome :

MyD88 cascade initiated on plasma membrane

Reactome :

MyD88 dependent cascade initiated on endosome

Reactome :

MyD88-independent cascade

Reactome :

MyD88:Mal cascade initiated on plasma membrane

Reactome :

NOD1/2 Signaling Pathway

Reactome :

Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways

Reactome :

Signaling by Interleukins

Reactome :

TAK1 activates NFkB by phosphorylation and activation of IKKs complex

Reactome :

Toll Like Receptor 10 (TLR10) Cascade

Reactome :

Toll Like Receptor 2 (TLR2) Cascade

Reactome :

Toll Like Receptor 3 (TLR3) Cascade

Reactome :

Toll Like Receptor 4 (TLR4) Cascade

Reactome :

Toll Like Receptor 5 (TLR5) Cascade

Reactome :

Toll Like Receptor 7/8 (TLR7/8) Cascade

Reactome :

Toll Like Receptor 9 (TLR9) Cascade

Reactome :

Toll Like Receptor TLR1:TLR2 Cascade

Reactome :

Toll Like Receptor TLR6:TLR2 Cascade

Reactome :

Toll-Like Receptors Cascades

Reactome :

TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation

Reactome :

TRAF6 Mediated Induction of proinflammatory cytokines

Reactome :

TRAF6 mediated induction of TAK1 complex

Reactome :

TRIF-mediated TLR3/TLR4 signaling

WikiPathway :

IL-1 signaling pathway

WikiPathway :

Integrated Breast Cancer Pathway

WikiPathway :

MAPK signaling pathway

WikiPathway :

MicroRNAs in cardiomyocyte hypertrophy

WikiPathway :

RANKL/RANK Signaling Pathway

WikiPathway :

Regulation of toll-like receptor signaling pathway

WikiPathway :

TGF beta Signaling Pathway

WikiPathway :

TNF alpha Signaling Pathway

WikiPathway :

Toll-like receptor signaling pathway

Protein

SMART domain :

PP2Cc : Serine/threonine phosphatases, family 2C, catalytic domain

Structural Annotation Wiki :

TOPSAN

UniProt :

Q8IZW2

UniProt :

Q15750

UniProt :

A8K6K3

HPRD :

04012

Clone

Cloning Information :

474567

HIP Master Clone ID :

396073

Original Clone ID :

1136.1.71_GO.37146

Experimental

TargetTrack - Experimental data :

GO.37146

Organism

Species Specific ID:

10454

Genome

Chromosome :

Map Location :

22q13.1

Ensembl :

ENSG00000100324

Reagents

Labome :

TAB1-antibody