GFRA3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  GFRA3
Gene Name:  GDNF family receptor alpha 3
Original Clone ID:
FLH166447.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 27620967
Title: A Pdx-1-regulated soluble factor activates rat and human islet cell proliferation
Authors:
HIP
HsCD00039690
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1200nts
Open reading frame: 1 to 1200

ATGGTGCGCCCCCTGAACCCGCGACCGCTGCCGCCCGTAGTCCTGATGTTGCTGCTGCTG
CTGCCGCCGTCGCCGCTGCCTCTCGCAGCCGGAGACCCCCTTCCCACAGAAAGCCGACTC
ATGAACAGCTGTCTCCAGGCCAGGAGGAAGTGCCAGGCTGATCCCACCTGCAGTGCTGCC
TACCACCACCTGGATTCCTGCACCTCTAGCATAAGCACCCCACTGCCCTCAGAGGAGCCT
TCGGTCCCTGCTGACTGCCTGGAGGCAGCACAGCAACTCAGGAACAGCTCTCTGATAGGC
TGCATGTGCCACCGGCGCATGAAGAACCAGGTTGCCTGCTTGGACATCTATTGGACCGTT
CACCGTGCCCGCAGCCTTGGTAACTATGAGCTGGATGTCTCCCCCTATGAAGACACAGTG
ACCAGCAAACCCTGGAAAATGAATCTCAGCAAACTGAACATGCTCAAACCAGACTCAGAC
CTCTGCCTCAAGTTTGCCATGCTGTGTACTCTCAATGACAAGTGTGACCGGCTGCGCAAG
GCCTACGGGGAGGCGTGCTCCGGGCCCCACTGCCAGCGCCACGTCTGCCTCAGGCAGCTG
CTCACTTTCTTCGAGAAGGCCGCCGAGCCCCACGCGCAGGGCCTGCTACTGTGCCCATGT
GCCCCCAACGACCGGGGCTGCGGGGAGCGCCGGCGCAACACCATCGCCCCCAACTGCGCG
CTGCCGCCTGTGGCCCCCAACTGCCTGGAGCTGCGGCGCCTCTGCTTCTCCGACCCGCTT
TGCAGATCACGCCTGGTGGATTTCCAGACCCACTGCCATCCCATGGACATCCTAGGAACT
TGTGCAACAGAGCAGTCCAGATGTCTACGAGCATACCTGGGGCTGATTGGGACTGCCATG
ACCCCCAACTTTGTCAGCAATGTCAACACCAGTGTTGCCTTAAGCTGCACCTGCCGAGGC
AGTGGCAACCTGCAGGAGGAGTGTGAAATGCTGGAAGGGTTCTTCTCCCACAACCCCTGC
CTCACGGAGGCCATTGCAGCTAAGATGCGTTTTCACAGCCAACTCTTCTCCCAGGACTGG
CCACACCCTACCTTTGCTGTGATGGCACACCAGAATGAAAACCCTGCTGTGAGGCCACAG
CCCTGGGTGCCCTCTCTTTTCTCCTGCACGCTTCCCTTGATTCTGCTCCTGAGCCTATGG

Protein sequence predicted based on nucleotide sequence

MVRPLNPRPLPPVVLMLLLLLPPSPLPLAAGDPLPTESRLMNSCLQARRKCQADPTCSAA
YHHLDSCTSSISTPLPSEEPSVPADCLEAAQQLRNSSLIGCMCHRRMKNQVACLDIYWTV
HRARSLGNYELDVSPYEDTVTSKPWKMNLSKLNMLKPDSDLCLKFAMLCTLNDKCDRLRK
AYGEACSGPHCQRHVCLRQLLTFFEKAAEPHAQGLLLCPCAPNDRGCGERRRNTIAPNCA
LPPVAPNCLELRRLCFSDPLCRSRLVDFQTHCHPMDILGTCATEQSRCLRAYLGLIGTAM
TPNFVSNVNTSVALSCTCRGSGNLQEECEMLEGFFSHNPCLTEAIAAKMRFHSQLFSQDW
PHPTFAVMAHQNENPAVRPQPWVPSLFSCTLPLILLLSLW

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1228
Start on reference sequence 26
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable