DNASU Plasmid | REXO4 (H. sapiens )<br>In pEU-His-FV (GST-tagged cell-free expression vector)

Explanation of Terms

Gene:

Gene Symbol: REXO4

Gene Name: REX4, RNA exonuclease 4 homolog (S. cerevisiae)

Original Clone ID:

5962.1.06_GO.35456

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pEU-His-FV

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00396990

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1296nts

Open reading frame: 15 to 1310

Reference Sequence Alignment

ATCATCATCATCACGCGATCGCCGAAAACCTGTACTTCCAGTCCGGGAAGGCGAAGGTCC
CCGCCTCCAAGCGCGCCCCGAGCAGCCCCGTGGCTAAGCCGGGTCCTGTCAAGACGCTCA
CTCGGAAGAAAAACAAGAAGAAAAAAAGGTTTTGGAAAAGCAAGGCGCGGGAAGTAAGCA
AGAAGCCAGCAAGCGGCCCCGGTGCTGTGGTGCGACCTCCAAAGGCACCAGAAGACTTTT
CTCAAAACTGGAAGGCGCTGCAAGAGTGGCTGCTGAAACAAAAATCTCAGGCCCCAGAAA
AGCCTCTTGTCATCTCTCAGATGGGTTCCAAAAAGAAGCCCAAAATTATCCAGCAAAACA
AAAAAGAGACCTCGCCTCAAGTGAAGGGAGAGGAGATGCCGGCAGGAAAAGACCAGGAGG
CCAGCAGGGGCTCTGTTCCTTCAGGTTCCAAGATGGACAGGAGGGCGCCAGTACCTCGCA
CCAAGGCCAGTGGAACAGAGCACAATAAGAAAGGAACCAAGGAAAGGACAAATGGTGATA
TTGTTCCAGAACGAGGGGACATCGAGCATAAGAAGCGGAAAGCTAAGGAGGCAGCCCCAG
CCCCACCCACCGAGGAAGACATCTGGTTTGACGACGTGGACCCAGCGGATATCGAAGCTG
CCATAGGTCCAGAGGCGGCCAAGATAGCGAGGAAACAGTTGGGTCAGAGCGAGGGCAGCG
TCAGCCTCAGCCTCGTGAAAGAGCAGGCCTTCGGCGGCCTGACAAGAGCCTTAGCCTTGG
ACTGTGAGATGGTGGGCGTGGGCCCTAAGGGGGAGGAGAGCATGGCCGCCCGTGTGTCCA
TCGTGAACCAGTATGGGAAGTGCGTTTATGACAAGTACGTCAAACCAACCGAGCCCGTGA
CGGACTATAGGACAGCGGTCAGTGGGATTCGGCCTGAGAACCTCAAGCAGGGAGAAGAGC
TTGAAGTTGTTCAGAAGGAAGTGGCAGAGATGCTGAAGGGCAGAATTCTAGTGGGGCACG
CTCTGCATAATGACCTAAAGGTACTATTTCTTGATCATCCAAAAAAGAAGATTCGGGACA
CACAGAAATATAAACCTTTCAAGAGTCAAGTAAAGAGTGGAAGGCCGTCTCTGAGACTAC
TTTCAGAGAAGATCCTTGGGCTCCAGGTCCAGCAGGCGGAGCACTGTTCAATTCAGGATG
CCCAGGCAGCAATGAGGCTGTACGTCATGGTGAAGAAGGAGTGGGAGAGCATGGCCCGAG
ACAGGCGCCCCCTGCTGACTGCTCCAGACCACTGCAGTGACGACGCCTAGTAGGTTTAAA
CGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACC

Protein sequence predicted based on nucleotide sequence

AIAENLYFQSGKAKVPASKRAPSSPVAKPGPVKTLTRKKNKKKKRFWKSKAREVSKKPAS
GPGAVVRPPKAPEDFSQNWKALQEWLLKQKSQAPEKPLVISQMGSKKKPKIIQQNKKETS
PQVKGEEMPAGKDQEASRGSVPSGSKMDRRAPVPRTKASGTEHNKKGTKERTNGDIVPER
GDIEHKKRKAKEAAPAPPTEEDIWFDDVDPADIEAAIGPEAAKIARKQLGQSEGSVSLSL
VKEQAFGGLTRALALDCEMVGVGPKGEESMAARVSIVNQYGKCVYDKYVKPTEPVTDYRT
AVSGIRPENLKQGEELEVVQKEVAEMLKGRILVGHALHNDLKVLFLDHPKKKIRDTQKYK
PFKSQVKSGRPSLRLLSEKILGLQVQQAEHCSIQDAQAAMRLYVMVKKEWESMARDRRPL
LTAPDHCSDDA*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in GO.35456

Recommended expression in:

Not Applicable

Vector Name:

pEU-His-FV

Synonyms:

Sequencing Primer:

Forward: pEU319 Forward

Reverse: pEU Reverse

Description:

Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI).

Comments:

Cloning into this vector will remove the BarCat casstte. To avoid, the toxicity of the barnase gene, the vector pEU-HSBC (EvNO00306667) can be ordered, which substitutes sacB for barnase.

Size (bp):

4966

Parent Vector:

None

Properties:

cell-free expression, multiple cloning site, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15782178

Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics

Vector Map:

Vector Sequence:

Sequence:

CACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCTGGCTAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTCCGGGTGCGGGACGGCGTTACCAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGGCGGCAGTTTACGAGAGAGATGATAGGGTCTGCTTCAGTAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAATACACGGAATTCGCGTTTTTATTTTTAATTTTCTTTCAAATACTTCTAGCTAGAGTATTTTTACAACAATTACCAACAACAACAACAAACAACAACAACATTACATTTTACATTCTACAACTACAGCCATGGGACATCATCATCATCATCACGCGATCGCCATGGAATAAGTAAAGGAATCACATGGCACAGGTTATCAACACGTTTGACGGGGTTGCGGATTATCTTCAGACATATCATAAGCTACCTGATAATTACATTACAAAATCAGAAGCACAAGCCCTCGGCTGGGTGGCATCAAAAGGGAACCTTGCAGACGTCGCTCCGGGGAAAAGCATCGGCGGAGACATCTTCTCAAACAGGGAAGGCAAACTCCCGGGCAAAAGCGGACGAACATGGCGTGAAGCGGATATTAACTATACATCAGGCTTCAGAAATTCAGACCGGATTCTTTACTCAAGCGACTGGCTGATTTACAAAACAACGGACCATTATCAGACCTTTACAAAAATCAGATAATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAAAAAACTAGTTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCTGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCCTATGCCGGTTCGACGGCTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCACTCACTGACTCGCTGCGCTCGGTCGCTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGCGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

2809

3491

gene fragment

Omega

TMV Omega

397

520

gene fragment

TMV-L

278

2238

gene fragment

BarCat-bar

BarCat cassette, including Barnase

572

908

gene fragment

BarCat-cat

BarCat cassette, including CAT

1010

1667

primer

His2 forward

His2 forward sequencing primer TCTTTCAAATACTTCTAGCTAGAGTA

424

449

promoter

SP6

SP6 promoter

373

390

recombination site

MCS

pF1K (Flexi-Vector) homology domain

1675

1803

selectable marker

AmpR

ampicillin resistance gene

3589

4248

tag

His

6xHis tag

527

544

Gene

Gene Symbol :

REXO4

Symbol Nomenclature :

REXO4

SYNONYM :

REX4; XPMC2; XPMC2H

Designation :

RNA exonuclease 4|XPMC2 prevents mitotic catastrophe 2 homolog|Xenopus prevents mitotic catastrophe 2 homolog|exonuclease XPMC2

Full Nomenclature :

REX4, RNA exonuclease 4 homolog (S. cerevisiae)

GENEID :

57109

Locus Tag :

RP11-244N20.5

GI :

14424509

GenBank Accession :

BC009274

HGNC :

12820

MIM :

602930

Vega :

OTTHUMG00000020870

Target GenBank:

BC009274

Reference Sequence Alignment

  

HsCD00396990      ATCATCATCATCACGCGATCGCCGAAAACCTGTACTTCCAGTCCGGGAAGGCGAAGGTCC
NM_020385.3       -----------------------------------------ATGGGGAAGGCGAAGGTCC
                                                           :  ****************

HsCD00396990      CCGCCTCCAAGCGCGCCCCGAGCAGCCCCGTGGCTAAGCCGGGTCCTGTCAAGACGCTCA
NM_020385.3       CCGCCTCCAAGCGCGCCCCGAGCAGCCCCGTGGCTAAGCCGGGTCCTGTCAAGACGCTCA
                  ************************************************************

HsCD00396990      CTCGGAAGAAAAACAAGAAGAAAAAAAGGTTTTGGAAAAGCAAGGCGCGGGAAGTAAGCA
NM_020385.3       CTCGGAAGAAAAACAAGAAGAAAAAAAGGTTTTGGAAAAGCAAGGCGCGGGAAGTAAGCA
                  ************************************************************

HsCD00396990      AGAAGCCAGCAAGCGGCCCCGGTGCTGTGGTGCGACCTCCAAAGGCACCAGAAGACTTTT
NM_020385.3       AGAAGCCAGCAAGCGGCCCCGGTGCTGTGGTGCGACCTCCAAAGGCACCAGAAGACTTTT
                  ************************************************************

HsCD00396990      CTCAAAACTGGAAGGCGCTGCAAGAGTGGCTGCTGAAACAAAAATCTCAGGCCCCAGAAA
NM_020385.3       CTCAAAACTGGAAGGCGCTGCAAGAGTGGCTGCTGAAACAAAAATCTCAGGCCCCAGAAA
                  ************************************************************

HsCD00396990      AGCCTCTTGTCATCTCTCAGATGGGTTCCAAAAAGAAGCCCAAAATTATCCAGCAAAACA
NM_020385.3       AGCCTCTTGTCATCTCTCAGATGGGTTCCAAAAAGAAGCCCAAAATTATCCAGCAAAACA
                  ************************************************************

HsCD00396990      AAAAAGAGACCTCGCCTCAAGTGAAGGGAGAGGAGATGCCGGCAGGAAAAGACCAGGAGG
NM_020385.3       AAAAAGAGACCTCGCCTCAAGTGAAGGGAGAGGAGATGCCGGCAGGAAAAGACCAGGAGG
                  ************************************************************

HsCD00396990      CCAGCAGGGGCTCTGTTCCTTCAGGTTCCAAGATGGACAGGAGGGCGCCAGTACCTCGCA
NM_020385.3       CCAGCAGGGGCTCTGTTCCTTCAGGTTCCAAGATGGACAGGAGGGCGCCAGTACCTCGCA
                  ************************************************************

HsCD00396990      CCAAGGCCAGTGGAACAGAGCACAATAAGAAAGGAACCAAGGAAAGGACAAATGGTGATA
NM_020385.3       CCAAGGCCAGTGGAACAGAGCACAATAAGAAAGGAACCAAGGAAAGGACAAATGGTGATA
                  ************************************************************

HsCD00396990      TTGTTCCAGAACGAGGGGACATCGAGCATAAGAAGCGGAAAGCTAAGGAGGCAGCCCCAG
NM_020385.3       TTGTTCCAGAACGAGGGGACATCGAGCATAAGAAGCGGAAAGCTAAGGAGGCAGCCCCAG
                  ************************************************************

HsCD00396990      CCCCACCCACCGAGGAAGACATCTGGTTTGACGACGTGGACCCAGCGGATATCGAAGCTG
NM_020385.3       CCCCACCCACCGAGGAAGACATCTGGTTTGACGACGTGGACCCAGCGGATATCGAAGCTG
                  ************************************************************

HsCD00396990      CCATAGGTCCAGAGGCGGCCAAGATAGCGAGGAAACAGTTGGGTCAGAGCGAGGGCAGCG
NM_020385.3       CCATAGGTCCAGAGGCGGCCAAGATAGCGAGGAAACAGTTGGGTCAGAGCGAGGGCAGCG
                  ************************************************************

HsCD00396990      TCAGCCTCAGCCTCGTGAAAGAGCAGGCCTTCGGCGGCCTGACAAGAGCCTTAGCCTTGG
NM_020385.3       TCAGCCTCAGCCTCGTGAAAGAGCAGGCCTTCGGCGGCCTGACAAGAGCCTTAGCCTTGG
                  ************************************************************

HsCD00396990      ACTGTGAGATGGTGGGCGTGGGCCCTAAGGGGGAGGAGAGCATGGCCGCCCGTGTGTCCA
NM_020385.3       ACTGTGAGATGGTGGGCGTGGGCCCTAAGGGGGAGGAGAGCATGGCCGCCCGTGTGTCCA
                  ************************************************************

HsCD00396990      TCGTGAACCAGTATGGGAAGTGCGTTTATGACAAGTACGTCAAACCAACCGAGCCCGTGA
NM_020385.3       TCGTGAACCAGTATGGGAAGTGCGTTTATGACAAGTACGTCAAACCAACCGAGCCCGTGA
                  ************************************************************

HsCD00396990      CGGACTATAGGACAGCGGTCAGTGGGATTCGGCCTGAGAACCTCAAGCAGGGAGAAGAGC
NM_020385.3       CGGACTATAGGACAGCGGTCAGTGGGATTCGGCCTGAGAACCTCAAGCAGGGAGAAGAGC
                  ************************************************************

HsCD00396990      TTGAAGTTGTTCAGAAGGAAGTGGCAGAGATGCTGAAGGGCAGAATTCTAGTGGGGCACG
NM_020385.3       TTGAAGTTGTTCAGAAGGAAGTGGCAGAGATGCTGAAGGGCAGAATTCTAGTGGGGCACG
                  ************************************************************

HsCD00396990      CTCTGCATAATGACCTAAAGGTACTATTTCTTGATCATCCAAAAAAGAAGATTCGGGACA
NM_020385.3       CTCTGCATAATGACCTAAAGGTACTATTTCTTGATCATCCAAAAAAGAAGATTCGGGACA
                  ************************************************************

HsCD00396990      CACAGAAATATAAACCTTTCAAGAGTCAAGTAAAGAGTGGAAGGCCGTCTCTGAGACTAC
NM_020385.3       CACAGAAATATAAACCTTTCAAGAGTCAAGTAAAGAGTGGAAGGCCGTCTCTGAGACTAC
                  ************************************************************

HsCD00396990      TTTCAGAGAAGATCCTTGGGCTCCAGGTCCAGCAGGCGGAGCACTGTTCAATTCAGGATG
NM_020385.3       TTTCAGAGAAGATCCTTGGGCTCCAGGTCCAGCAGGCGGAGCACTGTTCAATTCAGGATG
                  ************************************************************

HsCD00396990      CCCAGGCAGCAATGAGGCTGTACGTCATGGTGAAGAAGGAGTGGGAGAGCATGGCCCGAG
NM_020385.3       CCCAGGCAGCAATGAGGCTGTACGTCATGGTGAAGAAGGAGTGGGAGAGCATGGCCCGAG
                  ************************************************************

HsCD00396990      ACAGGCGCCCCCTGCTGACTGCTCCAGACCACTGCAGTGACGACGCCTAGTAGGTTTAAA
NM_020385.3       ACAGGCGCCCCCTGCTGACTGCTCCAGACCACTGCAGTGACGACGCCTAG----------
                  **************************************************          

HsCD00396990      CGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACC
NM_020385.3       -----------------------------------------