DNASU Plasmid | PRPF18 (H. sapiens )<br>In pEU-His-FV (GST-tagged cell-free expression vector)

Explanation of Terms

Gene:

Gene Symbol: PRPF18

Gene Name: pre-mRNA processing factor 18

Original Clone ID:

5962.1.09_GO.37632

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pEU-His-FV

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00397005

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1056nts

Open reading frame: 33 to 1088

Reference Sequence Alignment

CTACAGCCATGGGACATCATCATCATCATCACGCGATCGCCGAAAACCTGTACTTCCAGT
CCGACATTCTGAAATCAGAGATCCTTCGGAAGCGGCAGCTGGTGGAGGACAGGAACCTGC
TGGTGGAAAATAAAAAATATTTCAAGCGTAGTGAGCTCGCCAAAAAAGAAGAGGAAGCAT
ATTTTGAAAGATGTGGCTACAAGATACAGCCAAAAGAGGAGGACCAGAAACCATTAACTT
CATCGAATCCAGTGTTAGAACTTGAACTGGCAGAGGAAAAATTACCTATGACGCTTTCTA
GGCAAGAGGTCATCAGAAGATTGAGAGAAAGAGGAGAACCAATCAGACTATTTGGAGAGA
CTGATTATGATGCTTTTCAACGTTTAAGGAAAATAGAGATCCTCACACCAGAAGTTAACA
AGGGATTGAGGAATGATTTGAAAGCAGCCTTGGATAAGATTGATCAGCAGTACCTCAATG
AAATCGTCGGCGGTCAGGAGCCTGGAGAGGAAGACACACAGAATGATCTGAAAGTTCATG
AGGAAAACACCACAATTGAAGAGTTAGAGGCGCTTGGAGAGTCCTTAGGGAAAGGCGATG
ATCATAAGGACATGGACATCATCACCAAATTCCTGAAGTTTCTTCTTGGCGTTTGGGCTA
AAGAATTGAATGCCAGAGAAGATTATGTGAAACGCAGTGTGCAGGGTAAACTGAACAGTG
CGACCCAGAAACAGACCGAGTCCTACCTAAGACCACTTTTTAGAAAGCTACGGAAAAGGA
ATCTTCCTGCTGATATTAAAGAATCAATAACGGATATTATTAAATTCATGTTGCAGAGAG
AATACGTGAAGGCAAATGATGCTTATCTTCAGATGGCCATTGGAAATGCGCCTTGGCCCA
TCGGTGTCACTATGGTTGGTATCCATGCCAGAACTGGCAGAGAAAAGATTTTTTCCAAGC
ATGTTGCACATGTTTTAAATGACGAAACTCAGCGGAAATATATTCAGGGATTGAAGAGGT
TAATGACCATTTGCCAGAAACACTTTCCTACAGACCCATCCAAATGTGTGGAGTACAATG
CACTGTGATAGGTTTAAACGAATTCGAGCTCGGTACCC

Protein sequence predicted based on nucleotide sequence

AIAENLYFQSDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKIQP
KEEDQKPLTSSNPVLELELAEEKLPMTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLRK
IEILTPEVNKGLRNDLKAALDKIDQQYLNEIVGGQEPGEEDTQNDLKVHEENTTIEELEA
LGESLGKGDDHKDMDIITKFLKFLLGVWAKELNAREDYVKRSVQGKLNSATQKQTESYLR
PLFRKLRKRNLPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHAR
TGREKIFSKHVAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in GO.37632

Recommended expression in:

Not Applicable

Vector Name:

pEU-His-FV

Synonyms:

Sequencing Primer:

Forward: pEU319 Forward

Reverse: pEU Reverse

Description:

Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI).

Comments:

Cloning into this vector will remove the BarCat casstte. To avoid, the toxicity of the barnase gene, the vector pEU-HSBC (EvNO00306667) can be ordered, which substitutes sacB for barnase.

Size (bp):

4966

Parent Vector:

None

Properties:

cell-free expression, multiple cloning site, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15782178

Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics

Vector Map:

Vector Sequence:

Sequence:

CACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCTGGCTAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTCCGGGTGCGGGACGGCGTTACCAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGGCGGCAGTTTACGAGAGAGATGATAGGGTCTGCTTCAGTAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAATACACGGAATTCGCGTTTTTATTTTTAATTTTCTTTCAAATACTTCTAGCTAGAGTATTTTTACAACAATTACCAACAACAACAACAAACAACAACAACATTACATTTTACATTCTACAACTACAGCCATGGGACATCATCATCATCATCACGCGATCGCCATGGAATAAGTAAAGGAATCACATGGCACAGGTTATCAACACGTTTGACGGGGTTGCGGATTATCTTCAGACATATCATAAGCTACCTGATAATTACATTACAAAATCAGAAGCACAAGCCCTCGGCTGGGTGGCATCAAAAGGGAACCTTGCAGACGTCGCTCCGGGGAAAAGCATCGGCGGAGACATCTTCTCAAACAGGGAAGGCAAACTCCCGGGCAAAAGCGGACGAACATGGCGTGAAGCGGATATTAACTATACATCAGGCTTCAGAAATTCAGACCGGATTCTTTACTCAAGCGACTGGCTGATTTACAAAACAACGGACCATTATCAGACCTTTACAAAAATCAGATAATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAAAAAACTAGTTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCTGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCCTATGCCGGTTCGACGGCTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCACTCACTGACTCGCTGCGCTCGGTCGCTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGCGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

2809

3491

gene fragment

Omega

TMV Omega

397

520

gene fragment

TMV-L

278

2238

gene fragment

BarCat-bar

BarCat cassette, including Barnase

572

908

gene fragment

BarCat-cat

BarCat cassette, including CAT

1010

1667

primer

His2 forward

His2 forward sequencing primer TCTTTCAAATACTTCTAGCTAGAGTA

424

449

promoter

SP6

SP6 promoter

373

390

recombination site

MCS

pF1K (Flexi-Vector) homology domain

1675

1803

selectable marker

AmpR

ampicillin resistance gene

3589

4248

tag

His

6xHis tag

527

544

Gene

Gene Symbol :

PRPF18

Symbol Nomenclature :

PRPF18

SYNONYM :

PRP18; hPrp18

Designation :

PRP18 homolog|PRP18 pre-mRNA processing factor 18 homolog|PRP18 pre-mRNA processing factor 18 homolog(PRPF18)|pre-mRNA-splicing factor 18

Full Nomenclature :

pre-mRNA processing factor 18

GENEID :

8559

Locus Tag :

RP11-295P9.7

GI :

12653993

GenBank Accession :

BC000794

HGNC :

17351

MIM :

604993

Vega :

OTTHUMG00000017702

Target GenBank:

BC000794

Reference Sequence Alignment

  

HsCD00397005        CTACAGCCATGGGACATCATCATCATCATCACGCGATCGCCGAAAACCTGTACTTCCAGT
XM_005252634.1      -----------------------------------------------------------A
NM_003675.3         -----------------------------------------------------------A
                                                                               :

HsCD00397005        CCGACATTCTGAAATCAGAGATCCTTCGGAAGCGGCAGCTGGTGGAGGACAGGAACCTGC
XM_005252634.1      TGGACATTCTGAAATCAGAGATCCTTCGGAAGCGGCAGCTGGTGGAGGACAGGAACCTGC
NM_003675.3         TGGACATTCTGAAATCAGAGATCCTTCGGAAGCGGCAGCTGGTGGAGGACAGGAACCTGC
                      **********************************************************

HsCD00397005        TGGTGGAAAATAAAAAATATTTCAAGCGTAGTGAGCTCGCCAAAAAAGAAGAGGAAGCAT
XM_005252634.1      TGGTGGAAAATAAAAAATATTTCAAGCGTAGTGAGCTCGCCAAAAAAGAAGAGGAAGCAT
NM_003675.3         TGGTGGAAAATAAAAAATATTTCAAGCGTAGTGAGCTCGCCAAAAAAGAAGAGGAAGCAT
                    ************************************************************

HsCD00397005        ATTTTGAAAGATGTGG---------------------------CTACAAGATACAGCCAA
XM_005252634.1      ATTTTGAAAGATGTGGCTACAAGCCTATAAATGAGAAGCCATCTGGAGAGATACAGCCAA
NM_003675.3         ATTTTGAAAGATGTG---------------------------GCTACAAGATACAGCCAA
                    ***************                              ...************

HsCD00397005        AAGAGGAGGACCAGAAACCATTAACTTCATCGAATCCAGTGTTAGAACTTGAACTGGCAG
XM_005252634.1      AAGAGGAGGACCAGAAACCATTAACTTCATCGAATCCAGTGTTAGAACTTGAACTGGCAG
NM_003675.3         AAGAGGAGGACCAGAAACCATTAACTTCATCGAATCCAGTGTTAGAACTTGAACTGGCAG
                    ************************************************************

HsCD00397005        AGGAAAAATTACCTATGACGCTTTCTAGGCAAGAGGTCATCAGAAGATTGAGAGAAAGAG
XM_005252634.1      AGGAAAAATTACCTATGACGCTTTCTAGGCAAGAGGTCATCAGAAGATTGAGAGAAAGAG
NM_003675.3         AGGAAAAATTACCTATGACGCTTTCTAGGCAAGAGGTCATCAGAAGATTGAGAGAAAGAG
                    ************************************************************

HsCD00397005        GAGAACCAATCAGACTATTTGGAGAGACTGATTATGATGCTTTTCAACGTTTAAGGAAAA
XM_005252634.1      GAGAACCAATCAGACTATTTGGAGAGACTGATTATGATGCTTTTCAACGTTTAAGGAAAA
NM_003675.3         GAGAACCAATCAGACTATTTGGAGAGACTGATTATGATGCTTTTCAACGTTTAAGGAAAA
                    ************************************************************

HsCD00397005        TAGAGATCCTCACACCAGAAGTTAACAAGGGATTGAGGAATGATTTGAAAGCAGCCTTGG
XM_005252634.1      TAGAGATCCTCACACCAGAAGTTAACAAGGGATTGAGGAATGATTTGAAAGCAGCCTTGG
NM_003675.3         TAGAGATCCTCACACCAGAAGTTAACAAGGGATTGAGGAATGATTTGAAAGCAGCCTTGG
                    ************************************************************

HsCD00397005        ATAAGATTGATCAGCAGTACCTCAATGAAATCGTCGGCGGTCAGGAGCCTGGAGAGGAAG
XM_005252634.1      ATAAGATTGATCAGCAGTACCTCAATGAAATCGTCGGCGGTCAGGAGCCTGGAGAGGAAG
NM_003675.3         ATAAGATTGATCAGCAGTACCTCAATGAAATCGTCGGCGGTCAGGAGCCTGGAGAGGAAG
                    ************************************************************

HsCD00397005        ACACACAGAATGATCTGAAAGTTCATGAGGAAAACACCACAATTGAAGAGTTAGAGGCGC
XM_005252634.1      ACACACAGAATGATCTGAAAGTTCATGAGGAAAACACCACAATTGAAGAGTTAGAGGCGC
NM_003675.3         ACACACAGAATGATCTGAAAGTTCATGAGGAAAACACCACAATTGAAGAGTTAGAGGCGC
                    ************************************************************

HsCD00397005        TTGGAGAGTCCTTAGGGAAAGGCGATGATCATAAGGACATGGACATCATCACCAAATTCC
XM_005252634.1      TTGGAGAGTCCTTAGGGAAAGGCGATGATCATAAAGACATGGACATCATCACCAAATTCC
NM_003675.3         TTGGAGAGTCCTTAGGGAAAGGCGATGATCATAAAGACATGGACATCATCACCAAATTCC
                    **********************************.*************************

HsCD00397005        TGAAGTTTCTTCTTGGCGTTTGGGCTAAAGAATTGAATGCCAGAGAAGATTATGTGAAAC
XM_005252634.1      TGAAGTTTCTTCTTGGCGTTTGGGCTAAAGAATTGAATGCCAGAGAAGATTATGTGAAAC
NM_003675.3         TGAAGTTTCTTCTTGGCGTTTGGGCTAAAGAATTGAATGCCAGAGAAGATTATGTGAAAC
                    ************************************************************

HsCD00397005        GCAGTGTGCAGGGTAAACTGAACAGTGCGACCCAGAAACAGACCGAGTCCTACCTAAGAC
XM_005252634.1      GCAGTGTGCAGGGTAAACTGAACAGTGCGACCCAGAAACAGACCGAGTCCTACCTAAGAC
NM_003675.3         GCAGTGTGCAGGGTAAACTGAACAGTGCGACCCAGAAACAGACCGAGTCCTACCTAAGAC
                    ************************************************************

HsCD00397005        CACTTTTTAGAAAGCTACGGAAAAGGAATCTTCCTGCTGATATTAAAGAATCAATAACGG
XM_005252634.1      CACTTTTTAGAAAGCTACGGAAAAGGAATCTTCCTGCTGATATTAAAGAATCAATAACGG
NM_003675.3         CACTTTTTAGAAAGCTACGGAAAAGGAATCTTCCTGCTGATATTAAAGAATCAATAACGG
                    ************************************************************

HsCD00397005        ATATTATTAAATTCATGTTGCAGAGAGAATACGTGAAGGCAAATGATGCTTATCTTCAGA
XM_005252634.1      ATATTATTAAATTCATGTTGCAGAGAGAATACGTGAAGGCAAATGATGCTTATCTTCAGA
NM_003675.3         ATATTATTAAATTCATGTTGCAGAGAGAATACGTGAAGGCAAATGATGCTTATCTTCAGA
                    ************************************************************

HsCD00397005        TGGCCATTGGAAATGCGCCTTGGCCCATCGGTGTCACTATGGTTGGTATCCATGCCAGAA
XM_005252634.1      TGGCCATTGGAAATGCGCCTTGGCCCATCGGTGTCACTATGGTTGGTATCCATGCCAGAA
NM_003675.3         TGGCCATTGGAAATGCGCCTTGGCCCATCGGTGTCACTATGGTTGGTATCCATGCCAGAA
                    ************************************************************

HsCD00397005        CTGGCAGAGAAAAGATTTTTTCCAAGCATGTTGCACATGTTTTAAATGACGAAACTCAGC
XM_005252634.1      CTGGCAGAGAAAAGATTTTTTCCAAGCATGTTGCACATGTTTTAAATGACGAAACTCAGC
NM_003675.3         CTGGCAGAGAAAAGATTTTTTCCAAGCATGTTGCACATGTTTTAAATGACGAAACTCAGC
                    ************************************************************

HsCD00397005        GGAAATATATTCAGGGATTGAAGAGGTTAATGACCATTTGCCAGAAACACTTTCCTACAG
XM_005252634.1      GGAAATATATTCAGGGATTGAAGAGGTTAATGACCATTTGCCAGAAACACTTTCCTACAG
NM_003675.3         GGAAATATATTCAGGGATTGAAGAGGTTAATGACCATTTGCCAGAAACACTTTCCTACAG
                    ************************************************************

HsCD00397005        ACCCATCCAAATGTGTGGAGTACAATGCACTGTGATAGGTTTAAACGAATTCGAGCTCGG
XM_005252634.1      ACCCATCCAAATGTGTGGAGTACAATGCACTGTGA-------------------------
NM_003675.3         ACCCATCCAAATGTGTGGAGTACAATGCACTGTGA-------------------------
                    ***********************************                         

HsCD00397005        TACCC
XM_005252634.1      -----
NM_003675.3         -----

Pathways

WikiPathway :

mRNA processing

Protein

SMART domain :

SFM : Splicing Factor Motif, present in Prp18 and Pr04

Structural Annotation Wiki :

TOPSAN

UniProt :

Q99633

HPRD :

05407

Clone

Cloning Information :

510527

HIP Master Clone ID :

421694

Original Clone ID :

5962.1.09_GO.37632

Experimental

TargetTrack - Experimental data :

GO.37632

Organism

Species Specific ID:

8559

Genome

Chromosome :

Map Location :

10p13

Ensembl :

ENSG00000165630

Reagents

Labome :

PRPF18-antibody

PRPF18 (H. sapiens )In pEU-His-FV (GST-tagged cell-free expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

PRPF18 (H. sapiens )
In pEU-His-FV (GST-tagged cell-free expression vector)