Gene:
Gene Symbol:
dennd2db
Gene Name:
DENN/MADD domain containing 2Db
Original Clone ID:
5470.1.52_GO.79446
Keyword:
full-length cds
Species:
Danio rerio
Type:
cDNA
Vector Name:
pVP33K
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
DrCD00397105
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1473nts
Open reading frame: 51 to 1523
TGTTCCAGGGGCCCTGTTGCCCAGGTTGCTGTGAAAACCTGTACTTCCAGGCGATCGCCA
GCACAGAGAAGTGGAGGACAGGACTACAAATACAACAACTACACACTCCAACCGACAACG
TTGATCGCAGTGCCATGAAGAAGATCAGCTCCATGTTCTCAGCATTACAGGAGAAGTTCC
AGAGAGCAAAGGCCGGAGCCCCTACCCCCGCGGCCGTGGCGCCCCCTGCTGGACGGGAGA
GTCACTGTGGTCTGAGATTCTTTGAGTATCTGCTGGTGGTCAGTCTGAGGAAGAGGAGCA
GCGGGGTGGGGTACGAGCCACAGGTCACTTACCAGTTCCCTAAGCGTGAGGTGATGGGGC
GGCTGCAGCGCGAGCAGGAGGAGAAGAGCATGAAGGCGGCTCATCTGTTCTGCTTCCCTG
AGGGAGTCCACTGGGCCCCAGTGACAGAGTACAGCAGCGAGACGTTCTCCTTCGTTCTGA
CTGAAGTGGACGGCAGCCGCAGGAACGGCTACTGCAGGAGGCTGCTGCCGGCAGGTAAAG
GTGCTCGTCTTCCTGAAGCGTACTGCATCATCAGCCATGTGGCCTGTTTCGGCCTCTTCT
CAAAGATCTTTGATGAGGTGGAGAAGCGCCGGCAGATCTCCACAGCCATGATTTACCCGT
TCATGCAGAGTCTGCGGGAGGCACCGCTGCCTTCACCCGGCAGCACCGTCAGCATCAGCA
GCTTCATCCCGGACGCCGGCACACAGATCATCCGGCTGACGCGGCCTGCAGACTCCTGGC
TGGAGCATGTGGATTTCACTGCGCTTTTCAGCCGTCTGAACGATGAGGAGCTGCTGCTGG
TGTTCACCGCTGCTGTGCTGGAGCGACGAATCATCTTCATCTCAGAGGAGCTCAGCACTC
TCTCGCAGGTTCTTCATGCTGTAGCAGCGCTCCTGTATCCATTTGTGTGGCAGCACACGT
TTATATCAATCGTTCCTTCAGTTCTGATCGACGTTTGTGAAGCACCGACTCCATACCTGC
TGGGTGTTCAGAAGAGCATGATGGGGCTGATCACGGACCACAGCGACGTGATGATTGTTG
ATCTGTCGACTGGAGCGCCAACTAAATTCATCACAAGAATTGGAGATGAGGAGTCTCTGC
TGCCGCTGAAGCTGAAGGAAGAGCTGCTGTCGGGATTGTCCGCGCGCACGCACCACGCCT
CTACAGAGCAGCTGAACCGGCTGGTGTCAGAGGCCTTCCTGTCCGTATTCGTCAAGACTG
TCGGCCATTTCTCGGAGCACATTAAGCGTGGCGGGAAACCCCGGCAGTTCCAGAAGAAGA
GCTTCCTGAAAGCAGTGGAAAACAAAACCTACAGCAGCTTTGTTAAGCTCTTCATCCAGA
CGCAGATGTTTGACCTGTTCATACAGGAAGAGGAAACACAATCTAACCCTAATGGCTTCT
TTCACAGGAAGGTGAAGGAACATCGCGACAGGATGAAGCAGGAGAAGGCGAAGGCTGGGT
GGGTGCGAGGTGTGGTGATTTGATAGGTTTAAACGAATTCGAGCTCGGTA
Protein sequence predicted based on nucleotide sequence
AIASTEKWRTGLQIQQLHTPTDNVDRSAMKKISSMFSALQEKFQRAKAGAPTPAAVAPPA GRESHCGLRFFEYLLVVSLRKRSSGVGYEPQVTYQFPKREVMGRLQREQEEKSMKAAHLF CFPEGVHWAPVTEYSSETFSFVLTEVDGSRRNGYCRRLLPAGKGARLPEAYCIISHVACF GLFSKIFDEVEKRRQISTAMIYPFMQSLREAPLPSPGSTVSISSFIPDAGTQIIRLTRPA DSWLEHVDFTALFSRLNDEELLLVFTAAVLERRIIFISEELSTLSQVLHAVAALLYPFVW QHTFISIVPSVLIDVCEAPTPYLLGVQKSMMGLITDHSDVMIVDLSTGAPTKFITRIGDE ESLLPLKLKEELLSGLSARTHHASTEQLNRLVSEAFLSVFVKTVGHFSEHIKRGGKPRQF QKKSFLKAVENKTYSSFVKLFIQTQMFDLFIQEEETQSNPNGFFHRKVKEHRDRMKQEKA KAGWVRGVVI*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.79446
Recommended expression in:
Not Applicable
Vector Name:
pVP33K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte
Size (bp):
7253
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
5502
6184
promoter
T5
T5 promoter
123
169
protease cleavage site
TEV
TEV protease cleavage site
1415
1435
protease cleavage site
3C
3CP cleavage site
1373
1397
repressor protein gene
LacI
LacI cds
3842
4858
selectable marker
CmR
chloramphenicol resistance gene (CmR)
1900
2556
tag
His
6xHis tag
237
260
tag
MBP
MBP tag
267
1363
