Gene:
Gene Symbol:
Spsb2
Gene Name:
splA/ryanodine receptor domain and SOCS box containing 2
Original Clone ID:
5470.1.77_GO.79326
Keyword:
full-length cds
Species:
Mus musculus
Type:
cDNA
Vector Name:
pVP33K
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
MmCD00397108
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 783nts
Open reading frame: 51 to 833
TGTTCCAGGGGCCCTGTTGCCCAGGTTGCTGTGAAAACCTGTACTTCCAGGCGATCGCCG
GCCAGACGGCCCTGGCAAGGGGCAGCAGCAGCACCCCTACCTCGCAGGCTCTGTACTCGG
ACTTCTCTCCTCCCGAGGGCTTGGAGGAGCTCCTGTCTGCTCCCCCTCCTGACCTGGTTG
CCCAACGGCACCACGGCTGGAACCCCAAGGATTGCTCCGAGAACATCGATGTCAAGGAAG
GGGGTCTGTGCTTTGAGCGGCGCCCTGTGGCCCAGAGCACTGATGGAGTCCGGGGGAAAC
GGGGCTATTCGAGAGGTCTGCACGCCTGGGAGATCAGCTGGCCCCTGGAGCAAAGGGGCA
CACACGCCGTGGTGGGCGTGGCCACCGCCCTCGCCCCGCTGCAGGCTGACCACTATGCGG
CGCTTTTGGGCAGCAACAGCGAGTCCTGGGGCTGGGATATTGGGCGGGGAAAATTGTATC
ATCAGAGTAAGGGCCTCGAGGCCCCCCAGTATCCAGCTGGACCTCAGGGTGAGCAGCTAG
TGGTGCCAGAGAGACTGCTGGTGGTTCTGGACATGGAGGAGGGGACTCTTGGCTACTCTA
TTGGGGGCACGTACCTGGGACCAGCCTTCCGTGGACTGAAGGGGAGGACCCTCTATCCCT
CTGTAAGTGCTGTTTGGGGCCAGTGCCAGGTCCGCATCCGCTACATGGGCGAAAGAAGAG
GTGAGATACGGACTAGGTGTGGGGAGATCACTACTCTTGGCAATGGTTTGGGCTGGAAAC
TCATGGTTGGAGCACAGGAAGTAGGCTTCTTGTCACTTTGGCCTGTCACTTAGTAGGTTT
AAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
Protein sequence predicted based on nucleotide sequence
AIAGQTALARGSSSTPTSQALYSDFSPPEGLEELLSAPPPDLVAQRHHGWNPKDCSENID VKEGGLCFERRPVAQSTDGVRGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQAD HYAALLGSNSESWGWDIGRGKLYHQSKGLEAPQYPAGPQGEQLVVPERLLVVLDMEEGTL GYSIGGTYLGPAFRGLKGRTLYPSVSAVWGQCQVRIRYMGERRGEIRTRCGEITTLGNGL GWKLMVGAQEVGFLSLWPVT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.79326
Recommended expression in:
Not Applicable
Vector Name:
pVP33K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte
Size (bp):
7253
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
5502
6184
promoter
T5
T5 promoter
123
169
protease cleavage site
TEV
TEV protease cleavage site
1415
1435
protease cleavage site
3C
3CP cleavage site
1373
1397
repressor protein gene
LacI
LacI cds
3842
4858
selectable marker
CmR
chloramphenicol resistance gene (CmR)
1900
2556
tag
His
6xHis tag
237
260
tag
MBP
MBP tag
267
1363
