Gene:
Gene Symbol:
PEPE_0821
Gene Name:
rRNA methylase
Original Clone ID:
APC39068_SG262_pMCSG19B
Keyword:
full length
Species:
Pediococcus pentosaceus ATCC 25745
Type:
cDNA
Vector Name:
pMCSG19B
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jodi Carroll
PSI
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
PpCD00398435
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 819nts
Open reading frame: 49 to 867
TTCTGGGTGGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGCAAAAGAA
CGCGTAGATGTATTGCTGGTAGAACAGGGACTTTTTGATACCCGTGAAAAAGCAAAAAGA
GCCGTAATGGCCGGAGAAATTTTAGGAGACAACGAGGAGCGTTTGGATAAACCTGGTGTC
AAGATTGACCCTTCAGTTAAGTTACATTTAAAGGGTAAACCAATGCCCTATGTTAGCCGT
GGAGGCTTAAAATTAGAAAAAGCATTGAAAGTCTTTGGCCTTGATGTTAAGGGGCTTACA
GTATTAGATATAGGTTCTTCGACAGGTGGATTTACTGACGTTATGCTCCAAAATGGTGCG
AAGCTTAGTTATGCTTTAGATGTAGGAACTAATCAATTAGTATGGAAATTACGTGAGGAC
CCACGAGTGGTGGTAATGGAAAATACTAATTTTAGATACAGTAAATTAGAAGACTTTACT
GAGGGACAACCAGAGTTTGCATCAATTGATGTTTCATTTATCTCATTAAAACTAATTTTA
CCAGCGCTTAAAAATATTATTAAAACGCATGGATCAGTTGTCAGCTTGATTAAACCCCAG
TTTGAAGCCGGTAGAGAAAATGTTGGAAAGCATGGCATCGTTCGTGACCGTAAAGTTCAT
GAAGAGGTCCTTAAAAATGTACTAGCAATGGCCAATGCGGAAGGTTTCAACGTAAAGGGA
CTTGATTTTTCACCAATTAAGGGTGGAGAAGGCAATATCGAATTTTTAGCCTGGCTTGAA
CGATCCGATACGGATCAAGGAGTGATTGAAGAGAATGTTGATACCCAAAAAGTGATTGAT
AGTGCATATTCCAACTTAAATTCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGC
TCCGTCGACAAGCTTGCG
Protein sequence predicted based on nucleotide sequence
MAKERVDVLLVEQGLFDTREKAKRAVMAGEILGDNEERLDKPGVKIDPSVKLHLKGKPMP YVSRGGLKLEKALKVFGLDVKGLTVLDIGSSTGGFTDVMLQNGAKLSYALDVGTNQLVWK LREDPRVVVMENTNFRYSKLEDFTEGQPEFASIDVSFISLKLILPALKNIIKTHGSVVSL IKPQFEAGRENVGKHGIVRDRKVHEEVLKNVLAMANAEGFNVKGLDFSPIKGGEGNIEFL AWLERSDTDQGVIEENVDTQKVIDSAYSNLNS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC39068
Recommended expression in:
Not Applicable
Vector Name:
pMCSG19B
Synonyms:
''
Sequencing Primer:
Forward:
ANForward
Reverse:
T7 terminator
Description:
Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV cleavage site, His tag, and TEV protease cleavage site; ampicillin resistance; ligation independent cloning (LIC).
Comments:
Similar to pMCSG19C except location of the TVMV CDS. See http://bioinformatics.anl.gov/mcsg/technologies/vectors.html for more details
Size (bp):
7468
Parent Vector:
None
Properties:
bacterial expression
Author Name:
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
pBR322 origin of replication
5432
5432
gene fragment
TVMV cds
TVMV protease coding sequence
1881
2591
promoter
T7
T7 promoter
1515
1531
protease cleavage site
TVMV
TVMV cleavage site
286
306
protease cleavage site
TEV
TEV protease cleavage site
223
243
recombination site
LIC
Ligation Independent Cloning (LIC) recombination site
204
235
repressor binding site
lac operator
LacO fragment
1490
1512
repressor binding site
tet operator
TetO fragment
2610
2765
repressor protein gene
LacI
LacI cds
3072
4031
selectable marker
AmpR
Ampicillin resistance
6220
7080
tag
His
N terminal His tag
268
285
tag
MBP
Maltose binding protein tag
337
1143
trxn termination sequence
T7 term
T7 termination sequence
26
72
