Gene:
Gene Symbol:
PLXNB3
Gene Name:
plexin B3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069129)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399027
Price:
Login for Pricing
UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTGCCACGCCGCCCAGGAGACCCCTCTGCTGCACCACTTCATGGCCCCCGTGATGGCT
CGCTGGCCTCCCTTCGGCCTCTGCCTCCTCCTGCTGCTGCTGTCCCCACCGCCACTGCCC
TTGACAGGGGCCCATCGCTTCTCCGCACCTAATACCACTCTCAACCACTTGGCACTGGCA
CCTGGCCGAGGCACACTCTATGTCGGCGCAGTGAACCGCCTCTTCCAGCTCAGCCCCGAG
CTGCAGCTCGAGGCCGTGGCTGTCACTGGCCCTGTAATCGACAGCCCTGACTGCGTGCCC
TTCCGTGACCCAGCCGAGTGCCCACAGGCCCAGCTCACTGACAATGCCAACCAGCTGCTG
CTGGTGAGCAGCCGCGCCCAGGAGCTGGTGGCCTGCGGGCAGGTGCGGCAGGGCGTGTGT
GAGACACGGCGCCTTGGGGATGTGGCCGAGGTGCTGTACCAGGCTGAGGACCCTGGTGAC
GGGCAGTTTGTGGCTGCCAATACCCCGGGAGTGGCAACGGTGGGGCTGGTGGTGCCCTTG
CCCGGCCGGGACCTCCTGCTTGTGGCCAGAGGCCTGGCGGGCAAGCTGTCGGCAGGGGTG
CCACCCCTGGCCATCCGCCAGCTGGCCGGGTCTCAGCCCTTCTCCAGCGAGGGCCTGGGC
CGCCTGGTGGTGGGCGACTTCTCCGACTACAACAACAGCTACGTCGGGGCCTTTGCCGAC
GCCCGCTCCGCCTACTTCGTGTTCCGCCGCCGCGGGGCCCGGGCCCAGGCTGAGTACCGC
TCCTACGTGGCCCGCGTCTGCCTGGGGGACACCAACCTGTACTCCTACGTGGAGGTCCCC
CTCGCCTGCCAGGGCCAGGGCCTCATCCAGGCCGCCTTCCTTGCCCCGGGCACCTTGCTA
GGGGTGTTTGCCGCGGGCCCAAGGGGCACCCAGGCGGCGCTCTGTGCCTTCCCCATGGTG
GAGCTGGGTGCCAGCATGGAGCAGGCCCGGAGACTCTGCTACACGGCGGGCGGCCGGGGC
CCCAGCGGCGCAGAGGAAGCCACCGTGGAGTACGGCGTCACGTCGCGCTGCGTCACCCTG
CCCCTTGATTCCCCCGAGTCGTACCCCTGTGGCGACGAGCACACCCCCAGCCCCATTGCT
GGCCGCCAGCCCCTGGAGGTCCAGCCTCTGCTGAAGCTCGGGCAGCCGGTCAGCGCCGTG
GCAGCTCTCCAGGCAGATGGGCACATGATAGCCTTCCTGGGGGACACCCAGGGCCAGCTG
TACAAGGTCTTTCTCCACGGCTCCCAGGGCCAGGTTTACCACTCCCAGCAAGTGGGGCCT
CCAGGCTCAGCCATCAGCCCAGACCTGCTGCTGGACAGCAGTGGCAGTCACCTCTATGTC
CTGACTGCCCACCAGGTGGACCGGATACCTGTGGCAGCCTGCCCCCAGTTCCCTGACTGT
GCCAGCTGCCTCCAGGCCCAGGACCCGCTGTGTGGCTGGTGTGTCCTCCAGGGCAGGTGT
ACCCGGAAGGGCCAGTGCGGGCGGGCAGGCCAGCTGAACCAGTGGCTGTGGAGTTATGAG
GAGGACAGCCACTGCCTGCACATCCAGAGCCTGCTGCCGGGCCACCACCCCCGCCAGGAG
CAGGGCCAGGTCACTTTGTCTGTCCCCCGGCTGCCCATCCTGGATGCAGATGAATACTTC
CATTGTGCGTTCGGGGACTATGACAGCTTGGCTCATGTGGAAGGGCCCCACGTGGCCTGT
GTCACCCCTCCCCAAGACCAGGTGCCACTTAACCCTCCAGGCACAGACCACGTCACTGTG
CCCCTGGCCCTGATGTTCGAGGACGTGACTGTGGCTGCCACCAACTTCTCCTTTTATGAC
TGCAGTGCCGTCCAGGCCTTGGAGGCGGCTGCCCCGTGTCGCGCTTGCGTGGGCAGCATC
TGGCGGTGTCACTGGTGCCCGCAGAGTAGCCACTGCGTGTACGGAGAGCACTGCCCAGAG
GGCGAGAGGACCATCTACAGCGCCCAGGAGGTGGACATCCAGGTGCGTGGCCCAGGGGCT
TGCCCACAGGTCGAAGGCCTGGCAGGTCCCCACCTGGTGCCTGTGGGCTGGGAGAGCCAT
TTGGCCCTACGCGTGCGGAACCTTCAACATTTCCGAGGCCTGCCTGCCTCCTTCCACTGC
TGGCTGGAGCTGCCTGGAGAACTTCGGGGACTGCCGGCCACCCTGGAGGAGACAGCAGGG
GATTCAGGCCTCATCCACTGCCAGGCCCACCAGTTTTATCCCTCCATGTCCCAGCGGGAG
CTCCCAGTGCCCATCTACGTCACCCAGGGTGAAGCCCAGAGGCTGGACAACACCCATGCT
CTTTATGTGATCCTGTACGACTGCGCCATGGGCCACCCGGACTGCAGCCACTGCCAAGCG
GCCAACAGGAGCCTGGGCTGCCTGTGGTGTGCTGACGGCCAGCCTGCCTGTCGCTATGGG
CCCTTGTGCCCGCCGGGGGCTGTGGAGCTGCTGTGTCCTGCGCCCAGCATTGATGCAGTC
GAGCCCCTGACCGGTCCCCCTGAGGGAGGCTTGGCCCTCACCATCCTGGGCTCCAACCTG
GGCCGGGCCTTCGCCGATGTGCAGTACGCCGTGAGCGTGGCCAGCCGGCCCTGCAACCCT
GAGCCCTCTCTCTACCGCACGTCGGCCCGGATTGTGTGTGTGACATCTCCTGCCCCCAAT
GGCACCACTGGGCCCGTCCGGGTGGCCATTAAGAGCCAGCCACCAGGCATCTCAAGCCAG
CACTTCACCTACCAGGACCCTGTCCTGCTGAGCCTGAGTCCTCGCTGGGGCCCCCAGGCA
GGGGGCACCCAGCTCACCATCCGAGGTCAGCACCTCCAGACAGGTGGCAACACCAGTGCC
TTCGTGGGTGGCCAACCCTGTCCCATCCTGGAGCCAGTGTGTCCGGAGGCCATCGTGTGC
CGTACCAGGCCCCAGGCTGCCCCAGGAGAAGCAGCGGTCCTTGTGGTCTTTGGCCATGCC
CAGCGCACACTGCTCGCCAGCCCCTTCCGCTACACCGCCAACCCCCAGCTTGTAGCGGCG
GAGCCCAGTGCCAGCTTCCGGGGGGGTGGGCGACTGATCCGTGTCAGGGGCACCGGCCTA
GACGTGGTGCAGCGGCCCCTACTGTCTGTGTGGCTGGAGGCTGACGCAGAGGTGCAGGCT
TCCAGGGCCCAGCCCCAGGACCCACAGCCAAGGAGGAGCTGTGGAGCCCCTGCTGCGGAC
CCCCAGGCTTGTATCCAGCTCGGTGGGGGGCTGCTGCAGTGCTCCACCGTCTGCTCCGTC
AACTCGTCCAGCCTCCTCCTGTGCCGGAGCCCTGCTGTACCAGACAGAGCCCACCCGCAG
CGGGTCTTCTTCACCCTAGACAACGTGCAAGTGGACTTCGCCAGTGCCAGTGGGGGCCAG
GGCTTCCTGTACCAGCCCAACCCCCGCCTGGCACCCCTCAGCCGCGAGGGGCCTGCCCGC
CCCTACCGCCTCAAGCCAGGCCATGTCCTGGATGTGGAGGGCGAGGGCCTCAACCTGGGC
ATCAGCAAGGAGGAGGTGCGCGTGCACATCGGCCGCGGCGAGTGCCTGGTGAAGACGCTC
ACGCGCACCCACCTGTACTGCGAGCCGCCTGCGCACGCCCCGCAGCCTGCCAATGGCTCC
GGCCTGCCACAGTTCGTGGTGCAGATGGGCAATGTGCAGCTGGCCCTGGGCCCTGTGCAG
TACGAGGCTGAACCCCCGCTGTCTGCCTTTCCCGTGGAGGCCCAGGCAGGCGTGGGCATG
GGTGCTGCAGTGCTGATTGCCGCCGTGCTCCTCCTCACCCTCATGTACAGGCACAAGAGC
AAGCAGGCCCTGCGGGACTACCAGAAGGTGCTAGTGCAGCTGGAGAGCCTGGAGACCGGC
GTGGGAGACCAGTGCCGCAAGGAGTTCACAGACCTCATGACGGAGATGACCGACCTCAGC
AGCGACCTGGAGGGCAGCGGGATCCCCTTCCTGGACTACCGCACCTACGCCGAGCGCGCC
TTCTTCCCTGGCCATGGCGGTTGCCCGCTGCAGCCCAAGCCTGAGGGGCCAGGGGAGGAC
GGCCACTGTGCCACTGTGCGCCAGGGCCTCACGCAGCTCTCCAACCTGCTCAACAGCAAG
CTCTTCCTCCTCACGCTCATCCACACCCTGGAGGAGCAGCCCAGCTTTTCCCAGAGGGAT
CGCTGCCATGTGGCTTCGCTGCTGTCGCTAGCGCTACACGGCAAGCTGGAGTACCTGACG
GACATCATGAGGACCCTGCTGGGTGACCTGGCGGCCCATTACGTGCACAGGAACCCCAAG
CTCATGCTACGCAGGACAGAGACCATGGTGGAGAAACTGCTCACCAACTGGCTGTCCATC
TGCCTGTACGCCTTCCTGAGGGAGGTGGCTGGTGAACCACTGTACATGCTCTTCCGGGCC
ATCCAGTACCAGGTGGACAAAGGCCCCGTGGACGCCGTGACAGGCAAGGCCAAACGGACC
CTGAATGATAGCCGCTTGCTGCGGGAGGACGTGGAGTTCCAGCCCCTGACGCTGATGGTG
CTGGTGGGGCCCGGGGCTGGCGGGGCCGCAGGCAGCAGCGAGATGCAGCGCGTGCCAGCC
CGGGTGCTCGACACGGACACCATCACCCAGGTCAAGGAGAAGGTGTTGGACCAAGTCTAC
AAGGGCACCCCCTTCTCCCAGAGGCCCTCAGTGCATGCCCTAGACCTTGAGTGGCGCTCA
GGCCTGGCTGGTCACCTGACCCTATCGGACGAAGACTTGACCTCCGTGACCCAAAACCAC
TGGAAGAGACTCAACACCTTGCAACACTACAAGGTCCCAGATGGAGCAACAGTGGGGCTC
GTCCCTCAGCTGCACCGTGGCAGCACCATCTCCCAGAGCCTGGCCCAGAGGTGCCCCTTG
GGAGAGAACATACCCACGCTGGAGGATGGCGAGGAGGGGGGGGTGTGCCTCTGGCACCTG
GTGAAAGCCACCGAGGAGCCAGAAGGGGCCAAGGTGCGGTGCAGCAGCCTGCGGGAGCGC
GAGCCAGCAAGGGCCAAGGCCATTCCGGAAATCTACCTCACCCGTCTGCTGTCCATGAAG
GGCACGCTGCAGAAGTTTGTGGACGACACCTTCCAGGCCATTCTCAGCGTGAACCGGCCC
ATCCCCATCGCCGTCAAGTACCTGTTTGACCTTCTGGATGAGCTAGCAGAGAAGCACGGC
ATCGAGGACCCAGGGACCCTGCACATCTGGAAGACCAACAGTCTGCTGCTGCGGTTCTGG
GTGAATGCCTTGAAGAACCCACAGCTCATCTTTGATGTACGGGTGTCGGACAATGTGGAC
GCCATCCTTGCTGTCATCGCCCAGACCTTCATTGACTCCTGTACCACCTCGGAGCATAAA
GTGGGCCGGGATTCCCCAGTGAACAAACTGCTCTACGCCCGGGAGATCCCACGCTACAAG
CAGATGGTGGAGAGGTACTATGCGGACATTCGCCAGAGCTCTCCGGCGAGCTACCAGGAG
ATGAACTCTGCTTTGGCTGAGCTCTCCGGGAACTACACTTCTGCTCCCCACTGTCTGGAG
GCTCTGCAAGAACTCTACAACCACATCCACAGGTACTATGATCAGATTATCAGTGCCCTG
GAGGAGGACCCTGTGGGCCAGAAGCTGCAGCTGGCCTGCCGCCTGCAGCAGGTCGCCGCC
CTGGTGGAAAACAAAGTGACTGACCTGTAG
Protein sequence predicted based on nucleotide sequence
MCHAAQETPLLHHFMAPVMARWPPFGLCLLLLLLSPPPLPLTGAHRFSAPNTTLNHLALA PGRGTLYVGAVNRLFQLSPELQLEAVAVTGPVIDSPDCVPFRDPAECPQAQLTDNANQLL LVSSRAQELVACGQVRQGVCETRRLGDVAEVLYQAEDPGDGQFVAANTPGVATVGLVVPL PGRDLLLVARGLAGKLSAGVPPLAIRQLAGSQPFSSEGLGRLVVGDFSDYNNSYVGAFAD ARSAYFVFRRRGARAQAEYRSYVARVCLGDTNLYSYVEVPLACQGQGLIQAAFLAPGTLL GVFAAGPRGTQAALCAFPMVELGASMEQARRLCYTAGGRGPSGAEEATVEYGVTSRCVTL PLDSPESYPCGDEHTPSPIAGRQPLEVQPLLKLGQPVSAVAALQADGHMIAFLGDTQGQL YKVFLHGSQGQVYHSQQVGPPGSAISPDLLLDSSGSHLYVLTAHQVDRIPVAACPQFPDC ASCLQAQDPLCGWCVLQGRCTRKGQCGRAGQLNQWLWSYEEDSHCLHIQSLLPGHHPRQE QGQVTLSVPRLPILDADEYFHCAFGDYDSLAHVEGPHVACVTPPQDQVPLNPPGTDHVTV PLALMFEDVTVAATNFSFYDCSAVQALEAAAPCRACVGSIWRCHWCPQSSHCVYGEHCPE GERTIYSAQEVDIQVRGPGACPQVEGLAGPHLVPVGWESHLALRVRNLQHFRGLPASFHC WLELPGELRGLPATLEETAGDSGLIHCQAHQFYPSMSQRELPVPIYVTQGEAQRLDNTHA LYVILYDCAMGHPDCSHCQAANRSLGCLWCADGQPACRYGPLCPPGAVELLCPAPSIDAV EPLTGPPEGGLALTILGSNLGRAFADVQYAVSVASRPCNPEPSLYRTSARIVCVTSPAPN GTTGPVRVAIKSQPPGISSQHFTYQDPVLLSLSPRWGPQAGGTQLTIRGQHLQTGGNTSA FVGGQPCPILEPVCPEAIVCRTRPQAAPGEAAVLVVFGHAQRTLLASPFRYTANPQLVAA EPSASFRGGGRLIRVRGTGLDVVQRPLLSVWLEADAEVQASRAQPQDPQPRRSCGAPAAD PQACIQLGGGLLQCSTVCSVNSSSLLLCRSPAVPDRAHPQRVFFTLDNVQVDFASASGGQ GFLYQPNPRLAPLSREGPARPYRLKPGHVLDVEGEGLNLGISKEEVRVHIGRGECLVKTL TRTHLYCEPPAHAPQPANGSGLPQFVVQMGNVQLALGPVQYEAEPPLSAFPVEAQAGVGM GAAVLIAAVLLLTLMYRHKSKQALRDYQKVLVQLESLETGVGDQCRKEFTDLMTEMTDLS SDLEGSGIPFLDYRTYAERAFFPGHGGCPLQPKPEGPGEDGHCATVRQGLTQLSNLLNSK LFLLTLIHTLEEQPSFSQRDRCHVASLLSLALHGKLEYLTDIMRTLLGDLAAHYVHRNPK LMLRRTETMVEKLLTNWLSICLYAFLREVAGEPLYMLFRAIQYQVDKGPVDAVTGKAKRT LNDSRLLREDVEFQPLTLMVLVGPGAGGAAGSSEMQRVPARVLDTDTITQVKEKVLDQVY KGTPFSQRPSVHALDLEWRSGLAGHLTLSDEDLTSVTQNHWKRLNTLQHYKVPDGATVGL VPQLHRGSTISQSLAQRCPLGENIPTLEDGEEGGVCLWHLVKATEEPEGAKVRCSSLRER EPARAKAIPEIYLTRLLSMKGTLQKFVDDTFQAILSVNRPIPIAVKYLFDLLDELAEKHG IEDPGTLHIWKTNSLLLRFWVNALKNPQLIFDVRVSDNVDAILAVIAQTFIDSCTTSEHK VGRDSPVNKLLYAREIPRYKQMVERYYADIRQSSPASYQEMNSALAELSGNYTSAPHCLE ALQELYNHIHRYYDQIISALEEDPVGQKLQLACRLQQVAALVENKVTDL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
