Gene:
Gene Symbol:
SPTBN2
Gene Name:
spectrin, beta, non-erythrocytic 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069132)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399030
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGCAGCACGCTGTCACCCACAGACTTTGACAGCTTGGAAATCCAGGGCCAGTACAGT
GACATCAACAACCGCTGGGACCTTCCTGACTCGGACTGGGACAATGACAGCAGCTCGGCC
CGCCTCTTTGAGAGGTCTCGCATTAAGGCTCTGGCAGATGAACGAGAAGCTGTGCAGAAG
AAAACCTTCACCAAGTGGGTAAACTCGCACCTGGCCCGGGTCACGTGCCGGGTGGGGGAC
CTGTACAGCGACCTCCGGGACGGACGCAACCTGCTGAGGCTCCTCGAGGTGCTCTCGGGA
GAGATACTGCCAAAGCCTACAAAGGGCCGCATGCGGATCCACTGCCTGGAGAACGTGGAC
AAGGCACTGCAGTTCCTCAAGGAGCAGAAAGTGCACTTGGAAAACATGGGCTCCCATGAC
ATTGTGGACGGAAACCACCGACTGACCCTTGGGCTGGTCTGGACCATCATCCTTCGATTC
CAGATCCAAGACATCAGTGTGGAGACAGAAGACAACAAGGAGAAGAAGTCAGCCAAGGAT
GCCCTGCTTCTGTGGTGCCAGATGAAGACTGCAGGTTATCCCAACGTCAATGTACACAAC
TTCACCACCAGCTGGAGAGATGGACTAGCTTTCAACGCCATCGTGCATAAACACCGGCCA
GACCTGCTGGATTTTGAGTCTCTGAAGAAGTGTAATGCACACTATAATCTGCAGAATGCA
TTCAATCTGGCTGAAAAGGAACTGGGACTTACCAAGCTGCTGGATCCCGAAGACGTGAAT
GTGGACCAGCCAGATGAGAAGTCAATCATTACCTATGTGGCTACTTACTACCATTACTTC
TCCAAGATGAAGGCCCTGGCCGTGGAAGGCAAGAGAATTGGCAAGGTGCTGGACCATGCC
ATGGAGGCAGAGCGCCTGGTGGAGAAATACGAGTCCCTGGCCTCGGAGCTGCTGCAGTGG
ATCGAGCAAACGATCGTGACCCTCAATGACCGGCAGTTGGCCAACTCCCTTAGCGGGGTC
CAGAACCAGCTGCAGTCCTTCAACTCCTACCGCACCGTGGAGAAGCCGCCCAAGTTTACC
GAGAAAGGGAACTTGGAAGTGCTGCTCTTCACCATCCAGAGCAAGCTTCGGGCCAACAAC
CAGAAGGTCTACACGCCCCGCGAGGGCCGGCTCATCTCGGACATCAACAAGGCTTGGGAG
CGGCTGGAGAAGGCGGAGCACGAGCGTGAGCTGGCCCTGCGCACCGAGCTCATCCGCCAG
GAGAAGCTGGAGCAGCTGGCCGCCCGCTTCGACCGCAAGGCTGCCATGCGGGAGACCTGG
CTCAGCGAGAACCAGCGCCTCGTGTCCCAGGACAACTTTGGGCTGGAGCTGGCAGCTGTC
GAGGCAGCAGTACGGAAGCACGAAGCCATTGAGACGGACATCGTGGCCTACAGCGGCCGG
GTGCAGGCAGTGGACGCCGTGGCTGCAGAGCTGGCCGCCGAGCGCTACCACGACATCAAG
CGCATCGCCGCTCGGCAGCACAACGTGGCACGGCTCTGGGACTTCTTGCGGCAGATGGTG
GCCGCCCGGCGGGAGCGGCTCCTCCTCAACCTGGAGCTGCAGAAGGTGTTCCAGGACCTG
CTCTACCTCATGGACTGGATGGAAGAGATGAAGGGCCGGCTGCAGTCTCAGGACCTGGGC
AGGCACCTAGCAGGAGTGGAGGACCTGCTGCAGCTGCACGAGCTGGTGGAGGCAGACATC
GCCGTGCAGGCCGAGAGGGTGCGGGCCGTCAGCGCCTCTGCCCTGCGCTTCTGCAACCCA
GGGAAAGAGTATAGACCTTGCGACCCGCAGCTGGTGTCGGAGCGGGTGGCCAAGCTAGAG
CAGAGCTATGAGGCACTGTGCGAGTTGGCAGCGGCGCGGCGGGCCCGGCTGGAGGAATCA
CGGCGGCTCTGGCGTTTCCTCTGGGAGGTGGGTGAAGCTGAGGCCTGGGTGCGGGAGCAG
CAGCACCTCCTGGCCTCAGCCGACACGGGCCGAGACCTGACCGGTGCCCTCCGCCTGCTC
AACAAGCACACAGCCCTGCGGGGCGAGATGAGCGGCCGGCTGGGGCCCCTGAAGCTCACC
CTGGAGCAGGGCCAGCAGTTGGTGGCCGAGGGTCACCCTGGGGCAAGCCAGGCCTCTGCC
CGTGCAGCTGAACTCCAAGCCCAGTGGGAGCGGCTAGAGGCCCTGGCCGAGGAGCGTGCC
CAGCGGCTGGCCCAAGCCGCCAGCCTCTACCAGTTCCAGGCCGATGCAAACGACATGGAG
GCCTGGTTGGTTGACGCACTGCGCCTGGTGTCCAGCCCCGAGCTGGGGCACGACGAGTTC
TCCACGCAGGCTCTAGCCAGGCAGCATCGGGCCCTGGAGGAGGAGATTCGAAGCCACCGG
CCAACCCTGGACGCCTTGAGGGAACAGGCAGCAGCCCTGCCCCCCACACTGAGCCGCACG
CCCGAGGTGCAGGGCCGGGTGCCCACCCTGGAGCGGCACTACGAGGAGCTGCAGGCCCGG
GCAGGCGAGCGAGCGCGGGCCTTGGAGGCAGCCCTGGCGCTCTACACCATGCTCAGCGAG
GCCGGGGCCTGTGGACTCTGGGTGGAGGAGAAGGAGCAGTGGCTCAACGGGCTGGCCCTG
CCTGAACGCCTGGAGGACCTGGAGGTCGTGCAGCAGAGGTTCGAGACCCTGGAGCCTGAA
ATGAACACCCTTGCAGCACAAATCACCGCGGTGAATGACATTGCCGAGCAGTTACTGAAG
GCCAACCCCCCAGGCAAAGACCGCATTGTCAACACCCAGGAGCAGCTCAACCACAGGTGG
CAGCAGTTTCGGCGTCTGGCAGACGGCAAGAAGGCAGCTCTCACCTCAGCCCTGAGCATC
CAGAACTACCACTTAGAGTGCACGGAGACCCAGGCCTGGATGAGAGAGAAGACCAAAGTC
ATCGAGTCCACCCAGGGCCTAGGCAACGATCTGGCTGGGGTGCTGGCCCTGCAGCGCAAG
CTGGCCGGCACGGAGCGGGACCTGGAGGCCATCGCCGCCCGGGTGGGCGAACTGACTCGA
GAGGCAAATGCCCTGGCTGCCGGCCATCCCGCTCAGGCAGTGGCCATCAACGCCCGGCTG
AGAGAGGTGCAGACCGGCTGGGAGGACCTCAGGGCCACCATGCGGCGTCGAGAAGAGTCG
CTGGGGGAGGCGCGGCGGCTGCAGGACTTCTTGCGCAGCTTGGATGACTTCCAGGCCTGG
CTAGGCCGCACTCAGACTGCTGTGGCCTCTGAAGAAGGGCCGGCCACCCTGCCTGAGGCA
GAGGCCCTCCTGGCCCAACATGCAGCCCTGCGGGGAGAGGTGGAGCGGGCCCAGAGCGAG
TATAGCCGGCTGCGAGCCCTGGGCGAGGAGGTGACCCGGGACCAGGCTGACCCCCAGTGC
CTCTTCCTACGACAGCGACTGGAGGCCCTGGGAACTGGCTGGGAGGAGCTGGGCCGAATG
TGGGAGAGCCGGCAAGGTCGCCTGGCCCAGGCCCACGGCTTCCAGGGATTCCTGCGGGAT
GCTCGTCAGGCTGAGGGCGTGCTCAGCAGCCAGGAATATGTTCTGTCTCACACGGAGATG
CCAGGGACACTCCAGGCTGCTGATGCTGCCATTAAAAAACTGGAGGACTTCATGAGCACC
ATGGACGCCAATGGGGAACGGATCCACGGGCTCCTGGAGGCTGGCCGCCAGCTGGTATCT
GAAGGCAACATCCACGCCGACAAGATTCGGGAAAAGGCAGACTCCATTGAGAGGAGGCAC
AAGAAGAATCAAGACGCAGCGCAGCAATTTCTGGGCCGTCTTCGGGACAACCGGGAGCAG
CAGCATTTCCTGCAAGATTGTCACGAGCTGAAGCTCTGGATCGACGAGAAGATGCTGACA
GCCCAGGACGTGTCCTATGACGAGGCCCGCAACCTGCATACTAAGTGGCAGAAGCACCAG
GCATTCATGGCCGAGCTGGCTGCCAACAAAGACTGGCTGGACAAGGTGGACAAGGAAGGG
CGAGAGCTCACCCTTGAGAAGCCAGAGCTGAAAGCCCTGGTGTCGGAGAAGCTGAGAGAC
CTGCACAGGCGCTGGGACGAGCTGGAGACCACCACCCAAGCCAAGGCCCGCAGCCTCTTT
GATGCCAACCGAGCTGAGCTGTTTGCCCAGAGCTGCTGTGCCCTGGAGAGCTGGCTGGAG
AGCCTGCAGGCCCAGCTGCACTCGGATGACTACGGCAAGGACCTCACCAGCGTCAACATC
CTGCTCAAGAAGCAGCAGATGCTGGAATGGGAGATGGCTGTGAGAGAGAAGGAGGTGGAG
GCAATCCAGGCCCAGGCCAAAGCACTGGCCCAGGAGGACCAGGGTGCAGGGGAGGTGGAG
AGAACCTCGAGGGCCGTGGAGGAGAAGTTCAGGGCCTTGTGCCAGCCCATGCGGGAACGC
TGCCGGCGCCTGCAGGCTTCTCGCGAGCAGCACCAGTTCCACCGCGATGTGGAAGATGAG
ATTTTGTGGGTGACAGAGCGGCTGCCCATGGCCAGCTCCATGGAGCATGGCAAGGACCTG
CCCAGCGTCCAGCTTCTCATGAAGAAAAACCAGACCCTGCAGAAAGAGATTCAGGGCCAT
GAGCCCCGGATCGCGGACCTGAGGGAGCGGCAGCGTGCTCTAGGTGCAGCAGCAGCAGGT
CCAGAGCTGGCTGAGCTGCAGGAAATGTGGAAACGCCTGGGCCACGAGCTGGAACTTCGA
GGGAAGCGACTGGAGGATGCCCTGCGAGCCCAGCAGTTCTACCGCGATGCCGCCGAGGCG
GAGGCCTGGATGGGCGAGCAGGAATTACACATGATGGGCCAGGAGAAGGCCAAGGATGAG
CTGAGTGCCCAGGCAGAGGTGAAGAAGCACCAGGTGCTGGAGCAAGCCCTGGCCGACTAC
GCGCAGACCATCCACCAGCTGGCGGCCAGCAGCCAGGACATGATTGACCACGAGCACCCA
GAGAGCACTCGGATATCCATCCGCCAAGCCCAGGTGGACAAGCTGTATGCCGGCCTGAAG
GAGCTGGCTGGAGAGCGGCGGGAGCGCCTGCAGGAGCACCTCCGGCTGTGCCAGCTCCGC
CGCGAGCTGGATGACCTGGAACAGTGGATCCAGGAGCGCGAGGTGGTGGCGGCCTCCCAC
GAGCTGGGCCAGGACTACGAGCATGTGACTATGCTCCGAGACAAATTCCGAGAGTTCTCC
CGGGACACAAGCACCATCGGTCAGGAGCGCGTAGATAGCGCCAATGCGCTGGCCAATGGG
CTCATTGCTGGGGGCCATGCTGCACGGGCCACCGTGGCCGAGTGGAAGGACAGTCTCAAC
GAGGCCTGGGCTGACCTGCTTGAGCTGCTGGACACACGGGGTCAGGTGCTGGCCGCGGCG
TACGAGCTGCAGCGCTTCCTGCACGGGGCACGCCAAGCCCTGGCGCGGGTGCAGCACAAG
CAGCAGCAGCTTCCGGACGGGACTGGCCGCGACCTCAACGCTGCCGAGGCCCTGCAGCGC
CGACACTGTGCCTACGAGCATGACATTCAGGCCCTCAGCCCCCAGGTCCAGCAGGTGCAG
GACGACGGCCACCGGCTCCAGAAGGCCTACGCTGGAGACAAGGCTGAGGAGATCGGCCGC
CACATGCAGGCCGTGGCCGAGGCCTGGGCCCAGCTTCAGGGAAGCTCTGCCGCCCGCCGG
CAGCTGCTGCTGGACACCACAGACAAGTTCCGCTTCTTCAAGGCTGTCCGGGAACTGATG
CTCTGGATGGATGAGGTCAACCTGCAGATGGATGCCCAGGAGCGTCCCCGGGATGTGTCC
TCCGCGGATCTAGTCATCAAGAACCAGCAAGGCATCAAGGCAGAGATAGAGGCCCGGGCA
GACCGCTTCTCCTCCTGCATCGACATGGGGAAGGAGCTGCTGGCCAGGAGCCACTATGCG
GCCGAGGAGATCTCAGAGAAGCTGTCTCAGCTGCAGGCACGGCGCCAGGAGACAGCTGAG
AAGTGGCAGGAGAAGATGGACTGGCTTCAGCTGGTTTTGGAGGTGCTTGTGTTTGGAAGA
GATGCAGGGATGGCAGAGGCCTGGCTCTGCAGCCAGGAGCCACTGGTGCGCAGCGCTGAG
CTGGGTTGCACGGTCGACGAAGTTGAGAGCCTCATCAAGCGGCACGAGGCCTTCCAGAAG
TCAGCAGTGGCCTGGGAGGAGCGATTCTGTGCGCTGGAGAAGCTTACTGCGCTAGAGGAG
CGGGAGAAGGAGCGAAAGAGAAAGAGGGAGGAGGAGGAGCGGCGGAAACAGCCGCCTGCT
CCCGAACCCACAGCCAGTGTGCCTCCAGGGGACCTGGTGGGCGGCCAGACAGCTTCTGAC
ACCACCTGGGACGGAACCCAGCCACGGCCACCACCATCCACACAAGCACCCAGTGTTAAT
GGAGTCTGCACAGATGGAGAGCCCTCACAGCCCCTGCTGGGACAACAGAGACTTGAGCAC
AGCAGCTTCCCCGAAGGGCCGGGACCTGGCTCAGGGGACGAAGCCAATGGGCCCCGGGGA
GAGAGGCAGACCCGGACTCGGGGCCCGGCCCCATCTGCAATGCCCCAGAGCAGGTCTACC
GAGTCAGCCCATGCTGCCACCCTGCCGCCTCGAGGCCCAGAGCCATCTGCCCAGGAGCAG
ATGGAGGGGATGCTGTGCCGCAAGCAGGAGATGGAGGCCTTCGGGAAGAAGGCTGCCAAC
AGGTCCTGGCAGAACGTGTACTGTGTCCTGCGGCGTGGGAGCCTCGGCTTTTACAAGGAT
GCCAAGGCAGCCAGCGCGGGAGTGCCATACCACGGAGAAGTGCCTGTCAGCCTGGCCAGG
GCCCAGGGCAGCGTCGCCTTTGATTACCGAAAGCGCAAACATGTCTTCAAGCTGGGCTTA
CAGGATGGAAAAGAATATTTATTCCAGGCCAAGGATGAGGCAGAGATGAGCTCGTGGCTA
CGGGTGGTGAATGCAGCCATTGCCACAGCGTCTTCTGCCTCTGGAGAGCCTGAAGAGCCG
GTGGTGCCCAGCACCACCCGGGGCATGACCCGGGCCATGACCATGCCCCCAGTGTCACCC
GTCGGGGCTGAGGGGCCTGTTGTGCTCCGCAGCAAAGACGGCAGAGAACGAGAGCGAGAA
AAACGCTTCAGCTTCTTTAAGAAGAACAAGTAG
Protein sequence predicted based on nucleotide sequence
MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQK KTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGEILPKPTKGRMRIHCLENVD KALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKD ALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNA FNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHA MEAERLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFT EKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQ EKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGR VQAVDAVAAELAAERYHDIKRIAARQHNVARLWDFLRQMVAARRERLLLNLELQKVFQDL LYLMDWMEEMKGRLQSQDLGRHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCNP GKEYRPCDPQLVSERVAKLEQSYEALCELAAARRARLEESRRLWRFLWEVGEAEAWVREQ QHLLASADTGRDLTGALRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGASQASA RAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSSPELGHDEF STQALARQHRALEEEIRSHRPTLDALREQAAALPPTLSRTPEVQGRVPTLERHYEELQAR AGERARALEAALALYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPE MNTLAAQITAVNDIAEQLLKANPPGKDRIVNTQEQLNHRWQQFRRLADGKKAALTSALSI QNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAIAARVGELTR EANALAAGHPAQAVAINARLREVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAW LGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRALGEEVTRDQADPQC LFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEM PGTLQAADAAIKKLEDFMSTMDANGERIHGLLEAGRQLVSEGNIHADKIREKADSIERRH KKNQDAAQQFLGRLRDNREQQHFLQDCHELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQ AFMAELAANKDWLDKVDKEGRELTLEKPELKALVSEKLRDLHRRWDELETTTQAKARSLF DANRAELFAQSCCALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEWEMAVREKEVE AIQAQAKALAQEDQGAGEVERTSRAVEEKFRALCQPMRERCRRLQASREQHQFHRDVEDE ILWVTERLPMASSMEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLRERQRALGAAAAG PELAELQEMWKRLGHELELRGKRLEDALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDE LSAQAEVKKHQVLEQALADYAQTIHQLAASSQDMIDHEHPESTRISIRQAQVDKLYAGLK ELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFS RDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAA YELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSPQVQQVQ DDGHRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELM LWMDEVNLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGKELLARSHYA AEEISEKLSQLQARRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAE LGCTVDEVESLIKRHEAFQKSAVAWEERFCALEKLTALEEREKERKRKREEEERRKQPPA PEPTASVPPGDLVGGQTASDTTWDGTQPRPPPSTQAPSVNGVCTDGEPSQPLLGQQRLEH SSFPEGPGPGSGDEANGPRGERQTRTRGPAPSAMPQSRSTESAHAATLPPRGPEPSAQEQ MEGMLCRKQEMEAFGKKAANRSWQNVYCVLRRGSLGFYKDAKAASAGVPYHGEVPVSLAR AQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSASGEPEEP VVPSTTRGMTRAMTMPPVSPVGAEGPVVLRSKDGREREREKRFSFFKKNK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
