Gene:
Gene Symbol:
ABCA2
Gene Name:
ATP-binding cassette, sub-family A (ABC1), member 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069142)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399040
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGGAGAAAAACGAGCCGCGTGCAGCAGGGGCCGCCTCGGTCCCCTGCCTGCTCCGCT
CACGGCGAGCGTTCCTGGGGCCTCGAGTTTCCCCCCTTCCGCTGGCTGCTCGGAATCGCG
GGACGGACCCACCTGGCCGCCCTGCCTCCCTTCCAGTGGGTCCTGGCCTTCGAGATCTTC
ATCCCCCTGGTGCTGTTCTTTATCCTGCTGGGGCTGCGACAGAAGAAGCCCACCATCTCC
GTGAAGGAAGTCTCCTTCTACACAGCGGCGCCCCTGACGTCTGCCGGCATCCTGCCTGTC
ATGCAATCGCTGTGCCCGGACGGCCAGCGAGACGAGTTCGGCTTCCTGCAGTACGCCAAC
TCCACGGTCACGCAGCTGCTTGAGCGCCTGGACCGCGTGGTGGAGGAAGGCAACCTGTTT
GACCCAGCGCGGCCCAGCCTGGGCTCAGAGCTCGAGGCCCTACGCCAGCATCTGGAGGCC
CTCAGTGCGGGCCCGGGCACCTCGGGGAGCCACCTGGACAGATCCACAGTGTCTTCCTTC
TCTCTGGACTCGGTGGCCAGAAACCCGCAGGAGCTCTGGCGTTTCCTGACGCAAAACTTG
TCGCTGCCCAATAGCACGGCCCAAGCACTCTTGGCCGCCCGTGTGGACCCGCCCGAGGTC
TACCACCTGCTCTTTGGTCCCTCATCTGCCCTGGATTCACAGTCTGGCCTCCACAAGGGT
CAGGAGCCCTGGAGCCGCCTAGGGGGCAATCCCCTGTTCCGGATGGAGGAGCTGCTGCTG
GCTCCTGCCCTCCTGGAGCAGCTCACCTGCACGCCGGGCTCGGGGGAGCTGGGCCGGATC
CTCACTGTGCCTGAGAGTCAGAAGGGAGCCCTGCAGGGCTACCGGGATGCTGTCTGCAGT
GGGCAGGCTGCTGCGCGTGCCAGGCGCTTCTCTGGGCTGTCTGCTGAGCTCCGGAACCAG
CTGGACGTGGCCAAGGTCTCCCAGCAGCTGGGCCTGGATGCCCCCAACGGCTCGGACTCC
TCGCCACAGGCGCCACCCCCACGGAGGCTGCAGGCGCTTCTGGGGGACCTGCTGGATGCC
CAGAAGGTTCTGCAGGATGTGGATGTCCTGTCGGCCCTGGCCCTGCTACTGCCCCAGGGT
GCCTGCACTGGCCGGACCCCCGGACCCCCAGCCAGTGGTGCGGGTGGGGCGGCCAATGGC
ACTGGGGCAGGGGCAGTCATGGGCCCCAACGCCACCGCTGAGGAGGGCGCACCCTCTGCT
GCAGCACTGGCCACCCCGGACACGCTGCAGGGCCAGTGCTCAGCCTTCGTACAGCTCTGG
GCCGGCCTGCAGCCCATCTTGTGTGGCAACAACCGCACCATTGAACCCGAGGCGCTGCGG
CGGGGCAACATGAGCTCCCTGGGCTTCACGAGCAAGGAGCAGCGGAACCTGGGCCTCCTC
GTGCACCTCATGACCAGCAACCCCAAAATCCTGTACGCGCCTGCGGGCTCTGAGGTCGAC
CGCGTCATCCTCAAGGCCAACGAGACTTTTGCTTTTGTGGGCAACGTGACTCACTATGCC
CAGGTCTGGCTCAACATCTCGGCGGAGATCCGCAGCTTCCTGGAGCAGGGCAGGCTGCAG
CAACACCTGCGCTGGCTGCAGCAGTATGTAGCAGAGCTGCGGCTGCACCCCGAGGCACTG
AACCTGTCACTGGATGAGCTGCCGCCGGCCCTGAGACAGGACAACTTCTCGCTGCCCAGT
GGCATGGCCCTCCTGCAGCAGCTGGATACCATTGACAACGCGGCCTGCGGCTGGATCCAG
TTCATGTCCAAGGTGAGCGTGGACATCTTCAAGGGCTTCCCCGACGAGGAGAGCATTGTC
AACTACACCCTCAACCAGGCCTACCAGGACAACGTCACTGTTTTTGCCAGTGTGATCTTC
CAGACCCGGAAGGACGGCTCGCTCCCGCCTCACGTGCACTACAAGATCCGCCAGAACTCC
AGCTTCACCGAGAAAACCAACGAGATCCGCCGCGCCTACTGGCGGCCTGGGCCCAATACT
GGCGGCCGCTTCTACTTCCTCTACGGCTTCGTCTGGATCCAGGACATGATGGAGCGCGCC
ATCATCGACACTTTTGTGGGGCACGATGTGGTGGAGCCAGGCAGCTACGTGCAGATGTTC
CCCTACCCCTGCTACACACGCGATGACTTCCTGTTTGTCATTGAGCACATGATGCCGCTG
TGCATGGTGATCTCCTGGGTCTACTCCGTGGCCATGACCATCCAGCACATCGTGGCGGAG
AAGGAGCACCGGCTCAAGGAGGTGATGAAGACCATGGGCCTGAACAACGCGGTGCACTGG
GTGGCCTGGTTCATCACCGGCTTTGTGCAGCTGTCCATCTCCGTGACAGCACTCACCGCC
ATCCTGAAGTACGGCCAGGTGCTTATGCACAGCCACGTGGTCATCATCTGGCTCTTCCTG
GCAGTCTACGCGGTGGCCACCATCATGTTCTGCTTCCTGGTGTCTGTGCTGTACTCCAAG
GCCAAGCTGGCCTCGGCCTGCGGTGGCATCATCTACTTCCTGAGCTACGTGCCCTACATG
TACGTGGCGATCCGAGAGGAGGTGGCGCATGATAAGATCACGGCCTTCGAGAAGTGCATC
GCGTCCCTCATGTCCACGACGGCCTTTGGTCTGGGCTCTAAGTACTTCGCGCTGTATGAG
GTGGCCGGCGTGGGCATCCAGTGGCACACCTTCAGCCAGTCCCCGGTGGAGGGGGACGAC
TTCAACTTGCTCCTGGCTGTCACCATGCTGATGGTGGACGCCGTGGTCTATGGCATCCTC
ACGTGGTACATTGAGGCTGTGCACCCAGGCATGTACGGGCTGCCCCGGCCCTGGTACTTC
CCACTGCAGAAGTCCTACTGGCTGGGCAGTGGGCGGACAGAAGCCTGGGAGTGGAGCTGG
CCGTGGGCACGCACCCCCCGCCTCAGTGTCATGGAGGAGGACCAGGCCTGTGCCATGGAG
AGCCGGCGCTTTGAGGAGACCCGTGGCATGGAGGAGGAGCCCACCCACCTGCCTCTGGTT
GTCTGCGTGGACAAACTCACCAAGGTCTACAAGGACGACAAGAAGCTGGCCCTGAACAAG
CTGAGCCTGAACCTCTACGAGAACCAGGTGGTCTCCTTCTTGGGCCACAACGGGGCGGGC
AAGACCACCACCATGTCCATCCTGACCGGCCTGTTCCCTCCAACGTCGGGTTCCGCCACC
ATCTACGGGCACGACATCCGCACGGAGATGGATGAGATCCGCAAGAACCTGGGCATGTGC
CCGCAGCACAATGTGCTCTTTGACCGGCTCACGGTGGAGGAACACCTCTGGTTCTACTCA
CGGCTCAAGAGCATGGCTCAGGAGGAGATCCGCAGAGAGATGGACAAGATGATCGAGGAC
CTGGAGCTCTCCAACAAACGGCACTCACTGGTGCAGACATTGTCGGGTGGCATGAAGCGC
AAGCTGTCCGTGGCCATCGCCTTCGTGGGCGGCTCTCGCGCCATCATCCTGGACGAGCCC
ACGGCGGGCGTGGACCCCTACGCGCGCCGCGCCATCTGGGACCTCATCCTGAAGTACAAG
CCAGGCCGCACCATCCTTCTGTCCACCCACCACATGGATGAGGCTGACCTGCTTGGGGAC
CGCATTGCCATCATCTCCCATGGGAAGCTCAAGTGCTGCGGCTCCCCGCTCTTCCTCAAG
GGCACCTATGGCGACGGGTACCGCCTCACGCTGGTCAAGCGGCCCGCCGAGCCGGGGGGC
CCCCAAGAGCCAGGGCTGGCATCCAGCCCCCCAGGTCGGGCCCCGCTGAGCAGCTGCTCC
GAGCTCCAGGTGTCCCAGTTCATCCGCAAGCATGTGGCCTCCTGCCTGCTGGTCTCAGAC
ACAAGCACGGAGCTCTCCTACATCCTGCCCAGCGAGGCCGCCAAGAAGGGGGCTTTCGAG
CGCCTCTTCCAGCACCTGGAGCGCAGCCTGGATGCACTGCACCTCAGCAGCTTCGGGCTG
ATGGACACGACCCTGGAGGAAGTGTTCCTCAAGGTGTCGGAGGAGGATCAGTCGCTGGAG
AACAGTGAGGCCGATGTGAAGGAGTCCAGGAAGGATGTGCTCCCTGGGGCGGAGGGCCCG
GCGTCTGGGGAGGGTCACGCTGGCAATCTGGCCCGGTGCTCGGAGCTGACCCAGTCGCAG
GCATCGCTGCAGTCGGCGTCATCTGTGGGCTCTGCCCGTGGCGACGAGGGAGCTGGCTAC
ACCGACGTCTATGGCGACTACCGCCCCCTCTTTGATAACCCACAGGACCCAGACAATGTC
AGCCTGCAAGAGGTGGAGGCAGAGGCCCTGTCGAGGGTCGGCCAGGGCAGCCGCAAGCTG
GACGGCGGGTGGCTGAAGGTGCGCCAGTTCCACGGGCTGCTGGTCAAACGCTTCCACTGC
GCCCGCCGCAACTCCAAGGCACTCTTCTCCCAGATCTTGCTGCCAGCCTTCTTCGTCTGC
GTGGCCATGACCGTGGCCCTGTCCGTCCCGGAGATTGGTGATCTGCCCCCGCTGGTCCTG
TCACCTTCCCAGTACCACAACTACACCCAGCCCCGTGGCAATTTCATCCCCTACGCCAAC
GAGGAGCGCCGCGAGTACCGGCTGCGGCTATCGCCCGACGCCAGCCCCCAGCAGCTCGTG
AGCACGTTCCGGCTGCCGTCGGGGGTGGGTGCCACCTGCGTGCTCAAGTCTCCCGCCAAC
GGCTCGCTGGGGCCCACGTTGAACCTGAGCAGCGGGGAGTCGCGCCTGCTGGCGGCTCGG
TTCTTCGACAGCATGTGTCTGGAGTCCTTCACACAGGGGCTGCCACTGTCCAATTTCGTG
CCACCCCCACCCTCGCCCGCCCCATCTGACTCGCCAGCGTCCCCGGATGAGGACCTGCAG
GCCTGGAACGTCTCCCTGCCGCCCACCGCTGGGCCAGAAATGTGGACGTCGGCACCCTCC
CTGCCGCGCCTGGTACGGGAGCCCGTCCGCTGCACCTGCTCTGCGCAGGGCACCGGCTTC
TCCTGCCCCAGCAGTGTGGGCGGGCACCCGCCCCAGATGCGGGTGGTCACAGGCGACATC
CTGACCGACATCACCGGCCACAATGTCTCTGAGTACCTGCTCTTCACCTCCGACCGCTTC
CGACTGCACCGGTATGGGGCCATCACCTTTGGAAACGTCCTGAAGTCCATCCCAGCCTCA
TTTGGCACCAGGGCCCCACCCATGGTGCGGAAGATCGCGGTGCGCAGGGCTGCCCAGGTT
TTCTACAACAACAAGGGCTATCACAGCATGCCCACCTACCTCAACAGCCTCAACAACGCC
ATCCTGCGTGCCAACCTGCCCAAGAGCAAGGGCAACCCGGCGGCTTACGGCATCACCGTC
ACCAACCACCCCATGAATAAGACCAGCGCCAGCCTCTCCCTGGATTACCTGCTGCAGGGC
ACGGATGTCGTCATCGCCATCTTCATCATCGTGGCCATGTCCTTCGTGCCGGCCAGCTTC
GTTGTCTTCCTCGTGGCCGAGAAGTCCACCAAGGCCAAGCACCTGCAGTTTGTCAGCGGC
TGCAACCCCATCATCTACTGGCTGGCGAACTACGTGTGGGACATGCTCAACTACCTGGTC
CCCGCTACCTGCTGTGTCATCATCCTGTTTGTGTTCGACCTGCCGGCCTACACGTCGCCC
ACCAACTTCCCTGCCGTCCTCTCCCTCTTCCTGCTCTATGGGTGGTCCATCACGCCCATC
ATGTACCCGGCCTCCTTCTGGTTCGAGGTCCCCAGCTCCGCCTACGTGTTCCTCATTGTC
ATCAATCTCTTCATCGGCATCACCGCCACCGTGGCCACCTTCCTGCTACAGCTCTTCGAG
CACGACAAGGACCTGAAGGTTGTCAACAGTTACCTGAAAAGCTGCTTCCTCATTTTCCCC
AACTACAACCTGGGCCACGGGCTCATGGAGATGGCCTACAACGAGTACATCAACGAGTAC
TACGCCAAGATTGGCCAGTTTGACAAGATGAAGTCCCCGTTCGAGTGGGACATTGTCACC
CGCGGACTGGTGGCCATGGCGGTTGAGGGCGTCGTGGGCTTCCTCCTGACCATCATGTGC
CAGTACAACTTCCTGCGGCGGCCACAGCGCATGCCTGTGTCTACCAAGCCTGTGGAGGAT
GATGTGGACGTGGCCAGTGAGCGGCAGCGAGTGCTCCGGGGAGACGCCGACAATGACATG
GTCAAGATTGAGAACCTGACCAAGGTCTACAAGTCCCGGAAGATTGGCCGTATCCTGGCC
GTTGACCGCCTGTGCCTGGGTGTGCGTCCTGGCGAGTGCTTCGGGCTCCTGGGCGTCAAC
GGTGCGGGCAAGACCAGCACCTTCAAGATGCTGACCGGCGACGAGAGCACGACGGGGGGC
GAGGCCTTCGTCAATGGACACAGCGTGCTGAAGGAGCTGCTCCAGGTGCAGCAGAGCCTC
GGCTACTGCCCGCAGTGTGACGCGCTGTTCGACGAGCTCACGGCCCGGGAGCACCTGCAG
CTGTACACGCGGCTGCGTGGGATCTCCTGGAAGGACGAGGCCCGGGTGGTGAAGTGGGCT
CTGGAGAAGCTGGAGCTGACCAAGTACGCAGACAAGCCGGCTGGCACCTACAGCGGCGGC
AACAAGCGGAAGCTCTCCACGGCCATCGCCCTCATTGGGTACCCAGCCTTCATCTTCCTG
GACGAGCCCACCACAGGCATGGACCCCAAGGCCCGGCGCTTCCTCTGGAACCTCATCCTT
GACCTCATCAAGACAGGGCGTTCAGTGGTGCTGACATCACACAGCATGGAGGAGTGCGAG
GCGCTGTGCACGCGGCTGGCCATCATGGTGAACGGTCGCCTGCGGTGCCTGGGCAGCATC
CAGCACCTGAAGAACCGGTTTGGAGATGGCTACATGATCACGGTGCGGACCAAGAGCAGC
CAGAGTGTGAAGGACGTGGTGCGGTTCTTCAACCGCAACTTCCCGGAAGCCATGCTCAAG
GAGCGGCACCACACAAAGGTGCAGTACCAGCTCAAGTCGGAGCACATCTCGCTGGCCCAG
GTGTTCAGCAAGATGGAGCAGGTGTCTGGCGTGCTGGGCATCGAGGACTACTCGGTCAGC
CAGACCACACTGGACAATGTGTTCGTGAACTTTGCCAAGAAGCAGAGTGACAACCTGGAG
CAGCAGGAGACGGAGCCGCCATCCGCACTGCAGTCCCCTCTCGGCTGCTTGCTCAGCCTG
CTCCGGCCCCGGTCTGCCCCCACGGAGCTCCGGGCACTTGTGGCAGACGAGCCCGAGGAC
CTGGACACGGAGGACGAGGGCCTCATCAGCTTCGAGGAGGAGCGGGCCCAGCTGTCCTTC
AACACGGACACGCTCTGCTAG
Protein sequence predicted based on nucleotide sequence
MGRKTSRVQQGPPRSPACSAHGERSWGLEFPPFRWLLGIAGRTHLAALPPFQWVLAFEIF IPLVLFFILLGLRQKKPTISVKEVSFYTAAPLTSAGILPVMQSLCPDGQRDEFGFLQYAN STVTQLLERLDRVVEEGNLFDPARPSLGSELEALRQHLEALSAGPGTSGSHLDRSTVSSF SLDSVARNPQELWRFLTQNLSLPNSTAQALLAARVDPPEVYHLLFGPSSALDSQSGLHKG QEPWSRLGGNPLFRMEELLLAPALLEQLTCTPGSGELGRILTVPESQKGALQGYRDAVCS GQAAARARRFSGLSAELRNQLDVAKVSQQLGLDAPNGSDSSPQAPPPRRLQALLGDLLDA QKVLQDVDVLSALALLLPQGACTGRTPGPPASGAGGAANGTGAGAVMGPNATAEEGAPSA AALATPDTLQGQCSAFVQLWAGLQPILCGNNRTIEPEALRRGNMSSLGFTSKEQRNLGLL VHLMTSNPKILYAPAGSEVDRVILKANETFAFVGNVTHYAQVWLNISAEIRSFLEQGRLQ QHLRWLQQYVAELRLHPEALNLSLDELPPALRQDNFSLPSGMALLQQLDTIDNAACGWIQ FMSKVSVDIFKGFPDEESIVNYTLNQAYQDNVTVFASVIFQTRKDGSLPPHVHYKIRQNS SFTEKTNEIRRAYWRPGPNTGGRFYFLYGFVWIQDMMERAIIDTFVGHDVVEPGSYVQMF PYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMTIQHIVAEKEHRLKEVMKTMGLNNAVHW VAWFITGFVQLSISVTALTAILKYGQVLMHSHVVIIWLFLAVYAVATIMFCFLVSVLYSK AKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYE VAGVGIQWHTFSQSPVEGDDFNLLLAVTMLMVDAVVYGILTWYIEAVHPGMYGLPRPWYF PLQKSYWLGSGRTEAWEWSWPWARTPRLSVMEEDQACAMESRRFEETRGMEEEPTHLPLV VCVDKLTKVYKDDKKLALNKLSLNLYENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSAT IYGHDIRTEMDEIRKNLGMCPQHNVLFDRLTVEEHLWFYSRLKSMAQEEIRREMDKMIED LELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAIILDEPTAGVDPYARRAIWDLILKYK PGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKGTYGDGYRLTLVKRPAEPGG PQEPGLASSPPGRAPLSSCSELQVSQFIRKHVASCLLVSDTSTELSYILPSEAAKKGAFE RLFQHLERSLDALHLSSFGLMDTTLEEVFLKVSEEDQSLENSEADVKESRKDVLPGAEGP ASGEGHAGNLARCSELTQSQASLQSASSVGSARGDEGAGYTDVYGDYRPLFDNPQDPDNV SLQEVEAEALSRVGQGSRKLDGGWLKVRQFHGLLVKRFHCARRNSKALFSQILLPAFFVC VAMTVALSVPEIGDLPPLVLSPSQYHNYTQPRGNFIPYANEERREYRLRLSPDASPQQLV STFRLPSGVGATCVLKSPANGSLGPTLNLSSGESRLLAARFFDSMCLESFTQGLPLSNFV PPPPSPAPSDSPASPDEDLQAWNVSLPPTAGPEMWTSAPSLPRLVREPVRCTCSAQGTGF SCPSSVGGHPPQMRVVTGDILTDITGHNVSEYLLFTSDRFRLHRYGAITFGNVLKSIPAS FGTRAPPMVRKIAVRRAAQVFYNNKGYHSMPTYLNSLNNAILRANLPKSKGNPAAYGITV TNHPMNKTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSG CNPIIYWLANYVWDMLNYLVPATCCVIILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPI MYPASFWFEVPSSAYVFLIVINLFIGITATVATFLLQLFEHDKDLKVVNSYLKSCFLIFP NYNLGHGLMEMAYNEYINEYYAKIGQFDKMKSPFEWDIVTRGLVAMAVEGVVGFLLTIMC QYNFLRRPQRMPVSTKPVEDDVDVASERQRVLRGDADNDMVKIENLTKVYKSRKIGRILA VDRLCLGVRPGECFGLLGVNGAGKTSTFKMLTGDESTTGGEAFVNGHSVLKELLQVQQSL GYCPQCDALFDELTAREHLQLYTRLRGISWKDEARVVKWALEKLELTKYADKPAGTYSGG NKRKLSTAIALIGYPAFIFLDEPTTGMDPKARRFLWNLILDLIKTGRSVVLTSHSMEECE ALCTRLAIMVNGRLRCLGSIQHLKNRFGDGYMITVRTKSSQSVKDVVRFFNRNFPEAMLK ERHHTKVQYQLKSEHISLAQVFSKMEQVSGVLGIEDYSVSQTTLDNVFVNFAKKQSDNLE QQETEPPSALQSPLGCLLSLLRPRSAPTELRALVADEPEDLDTEDEGLISFEEERAQLSF NTDTLC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
