Gene:
Gene Symbol:
TECTA
Gene Name:
tectorin alpha
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069203)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399101
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATTATTCATCATTCCTTAGAATTTGGGTCTCTTTCATCTTCGCACTTGTACAGCAC
CAAGCTCAGCCCAGGGAGCTCATGTATCCATTTTGGCAGAATGACACCAAAACCCCTAAA
GTAGATGATGGAAGCTCATCTGAGATTAAGTTGGCCATCCCAGTTTTCTTCTTTGGCGTT
CCTTACCGCACTGTCTATGTCAATAACAACGGAGTTGTTTCCTTCAATGTGCTAGTGAGC
CAGTTCACGCCAGAATCCTTTCCCCTGACAGATGGGAGAGCCTTCGTCGCCCCATTTTGG
GCAGATGTGCACAATGGAATTCGAGGCGAGATCTATTACAGAGAGACCATGGAGCCTGCC
ATCTTGAAAAGAGCCACCAAGGACATCAGGAAGTACTTCAAAGACATGGCAACCTTCTCT
GCCACTTGGGTTTTCATTGTGACATGGGAGGAAGTCACGTTTTATGGAGGCAGCAGCACC
ACACCTGTGAACACCTTCCAGGCCGTCCTAGTGTCCGATGGCTCCTATACATTCACCCTC
TTCAATTATTACGAAATCAACTGGACCACGGGGACGGCGAGTGGCGGCGACCCCCTGACA
GGTCTTGGTGGAGTGATGGCACAGGCAGGATTTAATGGTGGAAACCTCACCAATTTCTTC
AGCCTCCCGGGGTCAAGAACCCCCGAGATCGTGAATATCCAGGAGACCACAAACGTCAAT
GTTCCAGGCCGCTGGGCATTTAAAGTTGATGGAAAGGAAATTGACCCAGCCAATGGCTGC
ACCTCAAGGGGACAATTCCTTCGGCGAGGGGAGGTGTTTTGGGATGACTTGAACTGCACC
GTCAAGTGCCGCTGTCTGGATTTCAACAATGAGATCTACTGCCAGGAGGCTTCCTGTAGC
CCCTACGAGGTGTGCGAACCCAAAGGCAAATTCTTCTACTGCAGCGCTGTGGAGACCAGC
ACATGCGTGGTGTTTGGGGAGCCACACTACCACACTTTTGACGGCTTCCTCTTCCACTTC
CAAGGCTCCTGTGCCTACTTGCTGGCCCGACAGTGTTTGCAGACTTCCAGCCTCCCTTTC
TTCAGTGTGGAGGCCAAGAATGAACACCGCAGAGGTTCAGCCGTCTCCTGGGTGAAGGAG
CTCTCAGTGGAGGTGAATGGCTACAAGATTCTCATCCCCAAAGGAAGCTATGGAAGAGTC
AAGGTTAATGACCTAGTGACTTCTTTGCCTGTCACCTTAGACTTGGGGACAGTGAAAATC
TACCAGAGTGGCATATCTACTGCCGTGGAAACAGATTTTGGGCTCTTAGTGACTTTTGAT
GGCCAGCACTACGCCTCCATTTCCGTCCCAGGCTCCTATATAAACTCCACCTGTGGACTC
TGTGGAAACTATAATAAAAACCCACTGGATGACTTCCTCCGCCCGGATGGCAGGCCGGCC
ATGTCTGTCCTGGATCTGGGAGAGAGCTGGCGTGTGTACCACGCAGACTGGAAGTGCGAC
TCCGGCTGCGTCGACAACTGCACCCAGTGCGACGCTGCCACTGAAGCCCTCTACTTTGGC
TCTGACTACTGCGGCTTCCTCAACAAGACAGACGGCCCTCTGTGGGAGTGTGGCACTGTC
GTGGACCCCACTGCTTTTGTGCACAGCTGCGTGTATGACCTGTGCAGTGTGAGGGACAAT
GGCACGCTCCTCTGCCAAGCCATCCAGGCCTATGCTCTTGTGTGCCAAGCCCTTGGCATT
CCAATTGGAGACTGGCGAACCCAGACTGGGTGTGTGTCCACAGTGCAGTGCCCGAGCTTC
AGCCACTACTCCGTGTGCACAAGCAGCTGCCCCGACACATGCTCCGACCTGACGGCCTCG
CGGAACTGCGCCACGCCGTGCACAGAGGGCTGCGAGTGCAACCAGGGCTTCGTCCTCAGC
ACCAGCCAGTGCGTCCCTCTGCACAAGTGCGGCTGCGACTTCGACGGCCACTACTACACC
ATGGGGGAGTTCTTCTGGGCCACGGCCAACTGCACTGTGCAATGCCTGTGCGAGGAGGGC
GGGGACGTCTACTGCTTCAACAAGACCTGCGGCAGCGGGGAGGTGTGCGCCGTGGAGGAC
GGCTACCAGGGCTGCTTCCCCAAGCGGGAGACCGTGTGCCTGCTCAGCCAGAACCAGGTG
CTGCACACCTTTGACGGCGCCTCCTACGCCTTCCCCTCCGAGTTCTCCTACACCCTCCTG
AAGACCTGCCCTGAGCGCCCAGAGTACTTGGAAATCGACATCAACAAGAAGAAGCCCGAT
GCAGGACCTGCTTGGCTGCGGGGACTTCGGATCCTGGTGGCCGACCAGGAGGTCAAGATA
GGAGGCATCGGGGCTTCGGAAGTCAAGTTGAATGGTCAGGAAGTGGAATTGCCTTTTTTC
CATCCTTCGGGGAAGCTGGAAATTTATCGAAACAAAAACAGTACGACAGTGGAGTCCAAG
GGCGTGGTGACTGTCCAGTACTCAGACATAGGTCTATTGTACATCCGGCTGTCCACCACA
TACTTCAATTGCACAGGGGGCTTGTGCGGCTTCTACAATGCCAACGCCAGTGACGAGTTC
TGTCTCCCCAACGGCAAGTGCACGGACAACCTGGCAGTGTTCCTGGAAAGCTGGACAACT
TTCGAGGAGATCTGCAATGGAGAGTGTGGGGACCTGCTGAAGGCCTGCAACAATGACTCG
GAGCTGCTCAAGTTTTATCGAAGCCGCTCCAGGTGCGGCATCATCAACGACCCCTCCAAC
AGCTCCTTCCTGGAGTGCCATGGGGTGGTGAACGTCACTGCCTATTACCGCACCTGCCTT
TTCCGCCTGTGCCAGAGTGGGGGCAATGAGTCAGAGCTCTGTGACTCTGTGGCCCGGTAT
GCAAGCGCCTGCAAGAATGCGGACGTGGAGGTGGGGCCCTGGCGGACCTATGACTTCTGC
CCACTGGAGTGCCCAGAGAACAGCCACTTTGAGGAGTGCATCACATGTACAGAGACCTGT
GAGACCCTTACCCTGGGCCCCATCTGCGTGGATAGCTGCTCTGAGGGATGTCAGTGTGAT
GAGGGCTATGCTCTACTGGGCAGCCAGTGTGTCACGCGGAGTGAGTGTGGCTGCAACTTT
GAGGGGCACCAACTTGCCACCAATGAGACCTTCTGGGTGGACCTGGACTGCCAGATCTTC
TGCTATTGCAGTGGCACAGACAACAGGGTCCACTGCGAGACCATTCCCTGCAAGGATGAT
GAGTACTGCATGGAGGAAGGTGGCCTGTACTACTGCCAAGCCCGCACCGACGCCTCCTGC
ATCGTCTCAGGCTACGGCCACTACCTCACCTTTGATGGCTTCCCCTTTGACTTCCAGACC
AGCTGCCCACTCATCCTGTGCACCACAGGAAGCAGGCCAAGCTCAGACTCTTTCCCCAAG
TTTGTTGTCACAGCCAAGAATGAGGACCGGGACCCGTCACTGGCCTTGTGGGTTAAGCAG
GTGGACGTGACCGTGTTTGGCTACAGCATCGTGATCCACCGAGCTTACAAGCACACTGTG
CTGGTCAACAGTGAACGGCTCTATCTGCCCCTGAAGCTGGGGCAAGGGAAGATAAATATC
TTTTCCTTTGGCTTCCACGTGGTGGTGGAAACTGATTTTGGCCTGAAGGTTGTATATGAC
TGGAAGACCTTCCTGTCCATCACAGTCCCTCGGAGCATGCAGAACAGCACCTATGGTCTG
TGTGGCCGCTACAACGGCAACCCTGATGATGACCTGGAGATGCCCATGGGTCTGCTTGCA
TCGAGTGTCAATGAGTTTGGGCAGAGCTGGGTGAAGAGGGACACCTTCTGCCAGGTGGGC
TGTGGGGACCGCTGTCCGTCCTGTGCCAAGGTGGAAGGTTTCTCCAAAGTGCAGCAGCTG
TGCAGCCTGATCCCCAACCAGAACGCTGCCTTCTCCAAGTGTCACAGCAAAGTTAACCCC
ACCTTCTTCTATAAGAACTGCCTGTTTGACTCTTGCATCGATGGGGGCGCGGTGCAGACC
GCCTGCAGCTGGCTGCAGAACTACGCCAGCACCTGCCAGACTCAGGGGATTACGGTGACT
GGCTGGAGGAATTACACGTCCTGCACTGTCACCTGCCCTCCAAACAGCCATTACGAGAGC
TGCGTGAGTGTCTGCCAGCCCCGCTGCGCCGCCATCCGCCTGAAGAGTGACTGCAGCCAC
TACTGCGTGGAGGGCTGTCACTGCGACGCTGGCTACGTCCTCAACGGCAAGAGCTGCATC
CTGCCCCACAGCTGCGGCTGCTACTCCGATGGCAAATATTACGAGCCCAAGCAGCTATTT
TGGAACAGCGACTGCACGCGGCGCTGCCGCTGTTTCCGTCGCAACGTGATTCAGTGCGAC
CCGCGCCAATGCAAGTCAGACGAGGAGTGTGCGCTGCGCAACGGGGTGCGCGGCTGCTTC
AGCACCAAGACCTCCTACTGCCTGGCGGCCGGCGGCGGCGTCTTCCGCACCTTCGACGGC
GCCTTCCTGCGCTTCCCAGCCAACTGCGCCTTCGTGCTGTCCACCATCTGCCAGAAACTG
CCCGACATCTCCTTCCAGCTTATCATCAACTTCGACAAGTGGTCGGCCCCCAACCTCACC
ATCATTTCGCCCGTCTACTTCTACATTAACGAAGAGCAGATTCTCATCAACGACCGGAAC
ACGGTCAAGGTGAATGGCACACAAGTGAATGTTCCATTTATAACTGGTTTGGCAACCAAA
ATCTACAGCAGTGAGGGGTTTCTGGTGATTGACACCAGCCCAGACATCCAGATATACTAC
AATGGTTTCAACGTCATTAAAATCAGCATCAGCGAGAGGCTGCAGAACAAAGTGTGCGGT
CTCTGTGGCAACTTCAACGGGGACCTAACAGATGATTATGTGACCTTGCGAGGGAAGCCG
GTGGTAAGCAGCGTGGTGCTGGCCCAGAGCTGGAAAACCAATGGCATGCAGAAGAGACCT
CTTGCCCCCAGCTGCAACGAGCTGCAGTTCTCACAGTATGCAGCCATGTGTGACAATGTG
CACATCCAGAAGATGCAGGGTGATGGCTACTGCCTGAAGCTCACCGACATGAAGGGCTTC
TTCCAGCCCTGCTATGGGCTTCTCGATCCCCTCCCATTCTACGAGTCCTGCTACCTGGAC
GGCTGCTACAGCCACAAGAAGTTCCAGCTGTGCGGCTCCCTGGCCGCCTACGGGGAGGCC
TGCCGCTCCTTCGGGATCCTTAGCACCGAGTGGATTGAGAAGGAGAATTGCTCAGGAGTG
GTTGAAGATCCCTGTGTGGGGGCGGACTGTCCCAACCGAACTTGCGAGCTGGGCAATGGC
AGGGAGCTGTGTGGCTGCATCGAGCCACCCCCCTATGGAAATAACTCACATGACATTATC
GATGCAGAGGTGACCTGCAAAGCAGCCCAAATGGAAGTGTCCATATCTAAGTGCAAGCTC
TTCCAGCTCGGTTTTGAGAGGGAGGGCGTGAGGATCAATGACAGACAGTGCACCGGCATC
GAGGGGGAAGATTTTATCTCCTTTCAGATCAACAACACCAAAGGGAATTGTGGAAACATT
GTGCAGTCCAATGGCACGCATATCATGTATAAAAACACACTCTGGATCGAAAGCGCCAAC
AACACTGGCAACATCATCACCAGGGACCGCACGATCAATGTGGAATTTTCATGTGCTTAT
GAGCTGGATATCAAGATCTCCTTGGATTCTGTTGTGAAGCCTATGCTAAGTGTAATTAAC
CTGACAGTTCCAACCCAAGAAGGCAGCTTCATCACCAAGATGGCTCTCTACAAAAACGCC
TCCTACAAACATCCTTACCGCCAGGGTGAAGTAGTGTTGACGACTCGAGATGTGCTGTAT
GTAGGGGTTTTTGTGGTTGGAGCTGACGCCACACATTTAATCCTAACACTCAACAAATGC
TATGCCACACCCACCCGAGATAGCAATGATAAGCTCCGATATTTCATCATTGAAGGAGGG
TGTCAGAACCTCAAAGATAACACCATTGGCATCGAGGAGAATGCAGTCTCCCTGACCTGT
CGCTTTCACGTCACCGTCTTTAAATTCATAGGGGATTACGACGAAGTTCACCTTCACTGT
GCAGTGTCACTCTGCGACTCAGAAAAGTACTCCTGTAAAATCACTTGCCCACACAATTCC
AGGATTGCCACAGATTACACAAAAGAGCCCAAAGAACAGATCATTTCAGTGGGACCTATT
AGGAGAAAAAGGCTGGACTGGTGTGAGGACAATGGAGGGTGTGAGCAGATTTGCACGAGC
CGGGTGGATGGGCCTCTCTGCAGCTGTGTAACAGGAACCCTGCAGGAGGACGGCAAGAGC
TGCAGAGCCTCTAATTCTTCAATGGAACTTCAAGTCTGGACGCTTCTTCTCATCATGATC
CAGATTTCATTATGGCATTTTGTCTATAAATCAGGCACGACCTCA
Protein sequence predicted based on nucleotide sequence
MNYSSFLRIWVSFIFALVQHQAQPRELMYPFWQNDTKTPKVDDGSSSEIKLAIPVFFFGV PYRTVYVNNNGVVSFNVLVSQFTPESFPLTDGRAFVAPFWADVHNGIRGEIYYRETMEPA ILKRATKDIRKYFKDMATFSATWVFIVTWEEVTFYGGSSTTPVNTFQAVLVSDGSYTFTL FNYYEINWTTGTASGGDPLTGLGGVMAQAGFNGGNLTNFFSLPGSRTPEIVNIQETTNVN VPGRWAFKVDGKEIDPANGCTSRGQFLRRGEVFWDDLNCTVKCRCLDFNNEIYCQEASCS PYEVCEPKGKFFYCSAVETSTCVVFGEPHYHTFDGFLFHFQGSCAYLLARQCLQTSSLPF FSVEAKNEHRRGSAVSWVKELSVEVNGYKILIPKGSYGRVKVNDLVTSLPVTLDLGTVKI YQSGISTAVETDFGLLVTFDGQHYASISVPGSYINSTCGLCGNYNKNPLDDFLRPDGRPA MSVLDLGESWRVYHADWKCDSGCVDNCTQCDAATEALYFGSDYCGFLNKTDGPLWECGTV VDPTAFVHSCVYDLCSVRDNGTLLCQAIQAYALVCQALGIPIGDWRTQTGCVSTVQCPSF SHYSVCTSSCPDTCSDLTASRNCATPCTEGCECNQGFVLSTSQCVPLHKCGCDFDGHYYT MGEFFWATANCTVQCLCEEGGDVYCFNKTCGSGEVCAVEDGYQGCFPKRETVCLLSQNQV LHTFDGASYAFPSEFSYTLLKTCPERPEYLEIDINKKKPDAGPAWLRGLRILVADQEVKI GGIGASEVKLNGQEVELPFFHPSGKLEIYRNKNSTTVESKGVVTVQYSDIGLLYIRLSTT YFNCTGGLCGFYNANASDEFCLPNGKCTDNLAVFLESWTTFEEICNGECGDLLKACNNDS ELLKFYRSRSRCGIINDPSNSSFLECHGVVNVTAYYRTCLFRLCQSGGNESELCDSVARY ASACKNADVEVGPWRTYDFCPLECPENSHFEECITCTETCETLTLGPICVDSCSEGCQCD EGYALLGSQCVTRSECGCNFEGHQLATNETFWVDLDCQIFCYCSGTDNRVHCETIPCKDD EYCMEEGGLYYCQARTDASCIVSGYGHYLTFDGFPFDFQTSCPLILCTTGSRPSSDSFPK FVVTAKNEDRDPSLALWVKQVDVTVFGYSIVIHRAYKHTVLVNSERLYLPLKLGQGKINI FSFGFHVVVETDFGLKVVYDWKTFLSITVPRSMQNSTYGLCGRYNGNPDDDLEMPMGLLA SSVNEFGQSWVKRDTFCQVGCGDRCPSCAKVEGFSKVQQLCSLIPNQNAAFSKCHSKVNP TFFYKNCLFDSCIDGGAVQTACSWLQNYASTCQTQGITVTGWRNYTSCTVTCPPNSHYES CVSVCQPRCAAIRLKSDCSHYCVEGCHCDAGYVLNGKSCILPHSCGCYSDGKYYEPKQLF WNSDCTRRCRCFRRNVIQCDPRQCKSDEECALRNGVRGCFSTKTSYCLAAGGGVFRTFDG AFLRFPANCAFVLSTICQKLPDISFQLIINFDKWSAPNLTIISPVYFYINEEQILINDRN TVKVNGTQVNVPFITGLATKIYSSEGFLVIDTSPDIQIYYNGFNVIKISISERLQNKVCG LCGNFNGDLTDDYVTLRGKPVVSSVVLAQSWKTNGMQKRPLAPSCNELQFSQYAAMCDNV HIQKMQGDGYCLKLTDMKGFFQPCYGLLDPLPFYESCYLDGCYSHKKFQLCGSLAAYGEA CRSFGILSTEWIEKENCSGVVEDPCVGADCPNRTCELGNGRELCGCIEPPPYGNNSHDII DAEVTCKAAQMEVSISKCKLFQLGFEREGVRINDRQCTGIEGEDFISFQINNTKGNCGNI VQSNGTHIMYKNTLWIESANNTGNIITRDRTINVEFSCAYELDIKISLDSVVKPMLSVIN LTVPTQEGSFITKMALYKNASYKHPYRQGEVVLTTRDVLYVGVFVVGADATHLILTLNKC YATPTRDSNDKLRYFIIEGGCQNLKDNTIGIEENAVSLTCRFHVTVFKFIGDYDEVHLHC AVSLCDSEKYSCKITCPHNSRIATDYTKEPKEQIISVGPIRRKRLDWCEDNGGCEQICTS RVDGPLCSCVTGTLQEDGKSCRASNSSMELQVWTLLLIMIQISLWHFVYKSGTTS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
