Gene:
Gene Symbol:
SRGAP3
Gene Name:
SLIT-ROBO Rho GTPase activating protein 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069053)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399143
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAA
ATAAAAGAGATCCGCACGCAGCTGGTGGAGCAGTTCAAATGTCTGGAGCAGCAATCAGAG
TCGCGACTGCAGCTGCTTCAAGACCTCCAGGAGTTTTTCCGCCGGAAAGCTGAGATTGAG
CTCGAGTACTCCCGCAGCCTGGAGAAGCTGGCTGAGCGCTTCTCCTCCAAAATCCGCAGC
TCCCGGGAGCACCAGTTCAAGAAGGACCAGTACCTCCTCTCGCCTGTGAACTGTTGGTAT
CTGGTTCTGCATCAGACCCGGCGGGAGAGCCGAGACCATGCCACCCTCAATGACATCTTC
ATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAGGATGTCATCAGACTCTTCAAA
AAGAGCAAGGAGATTGGCCTGCAGATGCACGAGGAGCTCCTGAAGGTGACCAATGAGCTC
TACACAGTCATGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCGGAAAGCAAG
CTGAAGGAGGCTGAGAAGCAGGAGGAGAAGCAGTTCAATAAGTCAGGAGACCTCAGCATG
AACCTGCTCCGGCACGAGGACCGGCCCCAGCGCCGCAGCTCTGTGAAGAAGATTGAGAAG
ATGAAGGAGAAGAGGCAGGCCAAGTACTCTGAGAACAAGCTGAAATGCACAAAGGCCCGG
AATGACTACTTGCTCAATCTGGCAGCCACCAACGCAGCTATAAGCAAATACTACATCCAT
GATGTCTCTGATCTGATCGATTGCTGTGATTTGGGCTTCCATGCCAGCCTGGCCCGCACC
TTCCGGACCTATCTCTCAGCTGAATACAACCTGGAGACCTCTCGCCACGAAGGGCTGGAT
GTCATTGAGAATGCAGTGGACAACCTGGATTCCCGAAGTGACAAGCACACAGTCATGGAC
ATGTGCAATCAAGTCTTCTGCCCTCCACTCAAGTTCGAGTTCCAGCCCCACATGGGGGAT
GAGGTCTGCCAGGTCAGCGCTCAGCAGCCCGTCCAGACAGAACTGCTCATGCGTTATCAC
CAGCTGCAGTCCAGACTGGCCACCCTCAAGATAGAGAATGAGGAGGTTAGGAAAACCCTG
GATGCCACCATGCAGACATTACAGGACATGCTGACTGTGGAGGACTTTGATGTCTCCGAT
GCCTTCCAACACAGTCGATCGACAGAGTCCGTCAAGTCGGCTGCCTCTGAGACCTACATG
AGCAAGATCAACATTGCCAAGAGGAGAGCCAACCAGCAGGAAACAGAAATGTTTTATTTT
ACAAAATTTAAAGAGTATGTGAATGGCAGTAACCTCATCACCAAGCTGCAGGCCAAGCAC
GATTTACTCAAGCAGACCCTGGGCGAAGGGGAAAGAGCAGAATGCGGCACCACCAGGCCC
CCCTGTCTTCCCCCTAAACCACAGAAAATGAGGAGACCTAGGCCTCTCTCAGTGTATAGC
CATAAACTCTTTAACGGCAGTATGGAAGCATTCATTAAGGATTCAGGACAAGCTATACCG
CTTGTAGTCGAGAGCTGCATCCGTTACATCAATTTATATGGACTCCAGCAGCAGGGGATC
TTCAGAGTGCCAGGATCTCAGGTGGAAGTCAATGACATCAAAAATTCCTTTGAGAGAGGT
GAAGACCCCCTTGTGGACGATCAAAATGAACGAGATATCAATTCAGTCGCTGGTGTTTTA
AAACTGTATTTCCGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTG
ATATCTACTATTAAACTGGAGAACCCAGCCGAGAGGGTGCACCAGATCCAACAAATCCTC
GTCACCCTTCCCCGCGTGGTCATTGTGGTCATGAGATACCTCTTCGCTTTCCTCAACCAC
CTCTCCCAGTATAGCGACGAGAACATGATGGATCCCTACAACCTGGCCATCTGCTTCGGG
CCTACCCTCATGCACATCCCTGATGGGCAGGACCCTGTGTCCTGCCAGGCACACATCAAT
GAAGTCATCAAAACCATCATCATCCATCATGAAGCCATCTTCCCCAGCCCCCGGGAGCTA
GAGGGACCTGTGTATGAAAAATGCATGGCTGGAGGGGAAGAATATTGTGACAGCCCACAC
AGCGAGCCAGGGGCCATCGATGAAGTTGACCATGACAATGGCACTGAGCCTCATACCAGC
GATGAAGAAGTGGAGCAGATCGAGGCTATTGCCAAGTTTGACTACATGGGGCGGTCCCCG
CGTGAGCTATCCTTCAAGAAGGGGGCCTCGCTGCTCCTGTACCACCGCGCCTCGGAGGAC
TGGTGGGAGGGCCGGCACAACGGCGTGGATGGACTCATCCCCCATCAGTACATAGTTGTA
CAGGACATGGATGATGCCTTCTCCGACAGCCTGAGCCAGAAGGCTGACAGCGAGGCCAGC
AGTGGGCCATTGCTGGATGACAAGGCCTCTTCCAAAAACGACCTCCAGTCCCCCACGGAG
CACATCTCGGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGCGGTTACGATCTGATGGA
GCAGCCATCCCCAGACGCCGAAGCGGGGGCGACACACACAGCCCGCCCCGGGGCCTGGGC
CCCAGCATAGACACACCACCCCGGGCTGCTGCCTGCCCCAGCAGCCCCCACAAAATCCCC
CTCACCCGGGGGAGGATCGAGAGCCCTGAGAAGCGGAGGATGGCGACGTTCGGGAGCGCT
GGCAGCATCAACTACCCTGACAAGAAGGCGCTCTCCGAAGGGCACTCGATGAGGTCGACC
TGCGGTTCCACCAGGCACAGCAGCCTAGGGGACCACAAGTCCCTGGAGGCCGAGGCCCTG
GCAGAAGACATCGAGAAGACCATGAGCACGGCTCTGCACGAGTTGCGGGAACTCGAGAGG
CAGAACACGGTCAAGCAGGCGCCAGATGTGGTGCTGGACACCCTGGAGCCCCTGAAGAAC
CCGCCAGGCCCCGTCAGCTCGGAGCCCGCCAGTCCCCTTCACACCATCGTCATCCGCGAC
CCCGATGCCGCCATGCGCCGCAGCAGCAGCTCCTCCACCGAGATGATGACCACCTTCAAG
CCAGCCCTGTCCGCCCGCCTGGCTGGCGCCCAGCTCCGCCCGCCCCCCATGCGGCCCGTG
CGGCCGGTGGTCCAGCACCGGTCCAGCAGCAGCAGCAGCTCGGGCGTGGGCAGCCCGGCC
GTGACGCCCACCGAGAAGATGTTCCCCAACAGCTCAGCGGACAAGTCGGGCACCATGTAG
Protein sequence predicted based on nucleotide sequence
MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIE LEYSRSLEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIF MNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESK LKEAEKQEEKQFNKSGDLSMNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKAR NDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLD VIENAVDNLDSRSDKHTVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYH QLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESVKSAASETYM SKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRP PCLPPKPQKMRRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRYINLYGLQQQGI FRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDL ISTIKLENPAERVHQIQQILVTLPRVVIVVMRYLFAFLNHLSQYSDENMMDPYNLAICFG PTLMHIPDGQDPVSCQAHINEVIKTIIIHHEAIFPSPRELEGPVYEKCMAGGEEYCDSPH SEPGAIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYMGRSPRELSFKKGASLLLYHRASED WWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTE HISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIP LTRGRIESPEKRRMATFGSAGSINYPDKKALSEGHSMRSTCGSTRHSSLGDHKSLEAEAL AEDIEKTMSTALHELRELERQNTVKQAPDVVLDTLEPLKNPPGPVSSEPASPLHTIVIRD PDAAMRRSSSSSTEMMTTFKPALSARLAGAQLRPPPMRPVRPVVQHRSSSSSSSGVGSPA VTPTEKMFPNSSADKSGTM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
