Gene:
Gene Symbol:
ARID4A
Gene Name:
AT rich interactive domain 4A (RBP1-like)
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069057)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399147
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGGCGGCAGATGAGCCTGCCTACCTGACAGTGGGAACCGATGTCAGTGCCAAGTAC
CGAGGTGCCTTCTGTGAGGCAAAGATTAAGACTGTGAAAAGGCTGGTGAAAGTTAAGGTA
CTCCTGAAACAGGATAATACCACACAATTGGTACAAGATGACCAAGTAAAGGGTCCTTTA
AGAGTTGGAGCTATTGTTGAAACAAGGACATCTGATGGATCTTTTCAGGAAGCTATTATC
AGCAAGTTGACAGATGCTAGTTGGTATACCGTGGTGTTTGATGATGGTGATGAGCGAACA
TTGAGACGTACCTCACTTTGTCTGAAAGGAGAGAGACATTTTGCAGAGAGTGAGACACTT
GACCAGCTTCCATTAACAAATCCAGAGCATTTTGGAACTCCAGTAATTGCAAAGAAGACG
AACAGAGGAAGGAGATCTTCTCTTCCTGTTACTGAAGATGAAAAGGAAGAAGAAAGCAGT
GAAGAGGAAGATGAAGACAAGCGCCGTCTCAATGATGAATTACTAGGAAAAGTTGTAAGT
GTGGTGTCTGCAACGGAGAGGACTGAATGGTATCCTGCTTTGGTAATATCTCCCAGCTGT
AATGATGACATCACAGTGAAAAAGGATCAGTGTTTAGTTCGATCATTTATTGATTCTAAA
TTTTACTCTATAGCAAGAAAGGACATTAAGGAAGTAGACATTCTCAATCTACCGGAATCT
GAGCTCTCCACTAAACCAGGGCTTCAGAAAGCAAGCATCTTCTTAAAAACTAGAGTTGTT
CCTGATAATTGGAAAATGGATATAAGTGAAATCCTTGAGTCATCCAGTAGTGATGATGAA
GATGGCCCAGCTGAAGAAAATGATGAAGAGAAGGAAAAGGAGGCCAAAAAGACAGAAGAA
GAGGTGCCTGAGGAAGAACTTGATCCTGAAGAGAGGGACAACTTCCTCCAGCAGCTTTAT
AAGTTTATGGAAGACAGAGGTACTCCAATCAACAAACCACCTGTTTTGGGCTATAAAGAT
CTCAATCTCTTCAAACTCTTCAGACTGGTTTATCATCAGGGTGGATGTGACAATATTGAT
AGTGGTGCTGTATGGAAGCAAATTTATATGGACCTTGGCATTCCTATTTTGAATTCAGCT
GCTTCCTACAATGTAAAAACTGCTTATAGAAAGTATCTCTATGGTTTTGAGGAGTACTGC
CGTTCGGCAAATATTCAGTTCAGAACTGTTCATCACCATGAACCAAAAGTAAAAGAGGAA
AAAAAAGACTTAGAAGAATCAATGGAAGAGGCTCTCAAATTAGATCAAGAAATGCCTTTA
ACAGAAGTGAAGAGTGAACCTGAGGAAAATATCGATTCAAACAGTGAAAGTGAAAGAGAA
GAGATAGAATTAAAATCTCCGAGGGGACGAAGGAGAATTGCTCGAGATGTAAATTCTATT
AAAAAGGAAATTGAAGAAGAGAAAACAGAAGACAAATTAAAAGATAATGATACAGAAAAT
AAGGATGTAGATGATGACTATGAAACTGCAGAGAAAAAAGAAAATGAGCTACTACTGGGG
AGAAAAAATACACCAAAGCAAAAAGAGAAGAAAATTAAAAAACAGGAGGATTCTGACAAA
GACTCAGATGAAGAGGAAGAGAAAAGCCAAGAGAGGGAAGAAACTGAAAGCAAATGTGAC
TCTGAAGGAGAGGAAGATGAGGAAGACATGGAACCCTGCCTAACAGGAACCAAAGTGAAA
GTAAAATATGGACGAGGGAAGACTCAGAAAATTTATGAAGCCAGTATTAAAAGCACTGAA
ATTGATGACGGAGAAGTTTTATATTTGGTACATTACTATGGATGGAATGTCAGGTATGAT
GAGTGGGTGAAGGCTGACAGGATAATCTGGCCTTTGGACAAAGGTGGACCAAAGAAAAAA
CAGAAGAAAAAAGCTAAAAATAAAGAAGATAGTGAAAAGGACGAAAAGAGAGATGAGGAG
AGGCAGAAGTCAAAACGGGGACGACCTCCTTTAAAATCAACCCTCTCATCAAACATGCCG
TATGGCTTATCTAAGACAGCAAACAGTGAAGGAAAATCAGACTCTTGTTCATCTGATAGT
GAAACAGAAGATGCTTTAGAAAAGAATTTAATAAATGAAGAACTTTCTCTTAAAGATGAA
CTAGAAAAAAATGAAAATTTGAATGATGATAAGCTAGATGAAGAAAATCCAAAGATTTCT
GCACATATATTAAAAGAAAATGATAGGACTCAAATGCAGCCTTTAGAAACCCTGAAGTTA
GAAGTTGGAGAGAATGAACAAATAGTACAGATTTTTGGGAACAAAATGGAAAAAACAGAA
GAAGTTAAGAAAGAAGCCGAAAAATCTCCAAAAGGAAAGGGAAGACGAAGCAAGACAAAA
GATCTTTCTTTAGAAATTATAAAGATTTCATCATTTGGCCAGAATGAAGCAGGAAGTGAA
CCTCATATAGAAGCTCATAGTCTTGAATTGTCTTCATTAGACAATAAAAACTTTTCTTCT
GCTACAGAAGATGAAATTGACCAATGTGTGAAAGAAAAGAAGTTGAAACGGAAAATACTA
GGACAATCATCGCCAGAGAAAAAAATAAGAATTGAGAATGGAATGGAAATGACAAATACT
GTATCTCAAGAAAGGACCAGTGATTGTATTGGATCTGAGGGAATGAAAAACTTAAATTTT
GAACAGCACTTTGAAAGAGAAAATGAAGGAATGCCATCATTGATAGCAGAGTCAAACCAA
TGCATCCAACAACTGACTAGTGAAAGATTTGATAGTCCAGCTGAAGAAACTGTAAATATT
CCACTAAAAGAAGATGAGGATGCAATGCCTCTGATCGGGCCTGAAACCTTGGTTTGCCAT
GAAGTAGATTTGGATGATTTGGATGAAAAGGATAAGACCAGCATTGAGGATGTAGCAGTT
GAAAGCTCTGAGTCTAACTCTCTTGTTTCTATTCCACCTGCCCTACCTCCTGTAGTCCAA
CATAACTTTTCAGTAGCTTCACCACTTACTCTTAGTCAAGATGAGTCTCGAAGCGTAAAA
AGTGAGAGTGATATAACGATTGAAGTTGATAGTATTGCTGAAGAATCTCAAGAAGGTCTC
TGTGAGAGGGAATCGGCAAATGGATTTGAAACTAATGTTGCCTCTGGTACCTGTAGTATA
ATTGTACAAGAGAGAGAGAGCAGAGAGAAGGGTCAGAAGAGGCCAAGTGATGGAAATAGT
GGATTAATGGCAAAAAAGCAAAAGCGTACCCCAAAGCGAACAAGTGCTGCAGCCAAAAAT
GAAAAGAATGGAACAGATGAATTAGATAATATGAACAGTACAGAGAGAATCTCATTTCTC
CAAGAAAAACTACAGGAAATCAGAAAATATTATATGTCTTTGAAGTCTGAAGTTGCAACC
ATAGACAGGAGGAGAAAAAGATTAAAAAAGAAAGACAGGGAAGTGTCTCATGCGGGAGCC
TCCATGTCATCTGCTTCATCAGACACTGGAATGAGTCCCTCATCATCATCTCCCCCACAA
AATGTACTTGCTGTAGAATGCAGGTAG
Protein sequence predicted based on nucleotide sequence
MKAADEPAYLTVGTDVSAKYRGAFCEAKIKTVKRLVKVKVLLKQDNTTQLVQDDQVKGPL RVGAIVETRTSDGSFQEAIISKLTDASWYTVVFDDGDERTLRRTSLCLKGERHFAESETL DQLPLTNPEHFGTPVIAKKTNRGRRSSLPVTEDEKEEESSEEEDEDKRRLNDELLGKVVS VVSATERTEWYPALVISPSCNDDITVKKDQCLVRSFIDSKFYSIARKDIKEVDILNLPES ELSTKPGLQKASIFLKTRVVPDNWKMDISEILESSSSDDEDGPAEENDEEKEKEAKKTEE EVPEEELDPEERDNFLQQLYKFMEDRGTPINKPPVLGYKDLNLFKLFRLVYHQGGCDNID SGAVWKQIYMDLGIPILNSAASYNVKTAYRKYLYGFEEYCRSANIQFRTVHHHEPKVKEE KKDLEESMEEALKLDQEMPLTEVKSEPEENIDSNSESEREEIELKSPRGRRRIARDVNSI KKEIEEEKTEDKLKDNDTENKDVDDDYETAEKKENELLLGRKNTPKQKEKKIKKQEDSDK DSDEEEEKSQEREETESKCDSEGEEDEEDMEPCLTGTKVKVKYGRGKTQKIYEASIKSTE IDDGEVLYLVHYYGWNVRYDEWVKADRIIWPLDKGGPKKKQKKKAKNKEDSEKDEKRDEE RQKSKRGRPPLKSTLSSNMPYGLSKTANSEGKSDSCSSDSETEDALEKNLINEELSLKDE LEKNENLNDDKLDEENPKISAHILKENDRTQMQPLETLKLEVGENEQIVQIFGNKMEKTE EVKKEAEKSPKGKGRRSKTKDLSLEIIKISSFGQNEAGSEPHIEAHSLELSSLDNKNFSS ATEDEIDQCVKEKKLKRKILGQSSPEKKIRIENGMEMTNTVSQERTSDCIGSEGMKNLNF EQHFERENEGMPSLIAESNQCIQQLTSERFDSPAEETVNIPLKEDEDAMPLIGPETLVCH EVDLDDLDEKDKTSIEDVAVESSESNSLVSIPPALPPVVQHNFSVASPLTLSQDESRSVK SESDITIEVDSIAEESQEGLCERESANGFETNVASGTCSIIVQERESREKGQKRPSDGNS GLMAKKQKRTPKRTSAAAKNEKNGTDELDNMNSTERISFLQEKLQEIRKYYMSLKSEVAT IDRRRKRLKKKDREVSHAGASMSSASSDTGMSPSSSSPPQNVLAVECR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
