Gene:
Gene Symbol:
TANC2
Gene Name:
tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069058)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399148
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTTTCGGAATAGTCTCAAGATGCTGCTTACTGGTGGGAAATCAAGTCGTAAAAACAGG
TCAAGTGATGGAGGGAGCGAGGAACCACCGGATCGAAGACAGTCAAGTGTAGACTCTCGC
CAAAGCCGCTCTGGGCAAGGTGGCATCTCCACAGAAAGCGACTGTGCTTTTGAGCCAGAC
TACGCTGTCCCGCCACTTCCAGTGAGTGAAGGTGATGCTGAACAGGAGCTTGGCCCCCCT
CCATCTGTAGATGAGGCAGCAAACACACTCATGACTCGTCTGGGTTTCCTCCTTGGAGAG
AAAGTCACAGAAGTTCAGCCAGGTGACCAATACAGCATGGAAGTACAGGATGAAAATCAG
ACCTCAGCAATCACCCAGCGGATAAGTCCTTGTTCCACTCTGACTAGCAGCACTGCCTCT
CCACCAGCCAGTAGCCCCTGCTCTACACTCCCACCCATCAGTACAAATGCAACTGCCAAG
GACTGCAGCTATGGGGCTGTTACTAGTCCAACCTCTACCCTTGAAAGCAGAGATAGTGGC
ATCATTGCTACATTAACAAGCTATTCTGAAAATGTGGAACGCACAAAATATGCTGGGGAA
AGCAGTAAAGAATTAGGATCTGGAGGAAACATAAAACCTTGGCAGTCTCAAAAATCCAGC
ATGGACTCCTGTTTGTATCGAGTAGATGAAAACATGACTGCTTCCACCTATAGTCTGAAT
AAGATCCCAGAGAGAAATTTGGAAACAGTGTTATCTCAATCAGTACAGTCTATTCCTTTG
TATCTCATGCCACGGCCAAATTCAGTAGCAGCCACCAGCTCTGCCCACTTGGAAGACCTT
GCTTACCTGGATGAGCAGAGACATACACCTTTGAGAACTTCTCTACGAATGCCAAGACAG
AGCATGGGTGGTGCTCGCACACAGCAGGATTTAAGAGTTCGCTTTGCACCTTATAGGCCT
CCAGATATCTCCTTGAAGCCTCTGCTCTTTGAAGTGCCCAGCATCACTACAGAGTCAGTG
TTTGTTGGCCGAGATTGGGTTTTCCACGAAATAGATGCTCAACTTCAAAGTTCAAATGCC
AGCGTGAACCAAGGAGTAGTGATTGTGGGAAACATTGGATTCGGCAAAACTGCCATCATC
TCCAGACTGGTGGCCCTCAGCTGCCATGGTACAAGGATGAGACAGATCGCCTCAGACAGC
CCACATGCCTCCCCCAAACATGTGGATGCCAACAGAGAGCTGCCACTCACACAGCCACCT
TCAGCCCACTCATCTATCACCAGTGGAAGCTGCCCAGGAACTCCGGAAATGCGCAGGCGG
CAGGAGGAGGCTATGCGAAGACTAGCCTCGCAGGTGGTTGCCTATCACTATTGTCAAGCA
GATAATGCCTACACTTGCTTGGTGCCAGAATTTGTCCACAATGTTGCTGCCTTGCTCTGC
CGCTCACCTCAGCTGACAGCCTATCGGGAGCAGCTTCTTCGGGAACCTCACCTGCAGAGC
ATGCTGAGCCTTCGTTCCTGTGTTCAAGACCCCATGGCCTCCTTCCGGAGGGGAGTTCTG
GAGCCACTAGAAAATCTCCATAAAGAGAGAAAAATCCCAGATGAAGATTTCATCATTTTA
ATTGATGGATTAAATGAAGCAGAATTTCACAAACCGGATTATGGGGATACAATTGTATCG
TTTCTGAGTAAAATGATCGGAAAGTTTCCTTCTTGGCTCAAACTAATTGTAACAGTTAGG
ACCAGTTTACAGGAAATTACCAAGCTGCTGCCTTTCCATAGGATTTTTTTGGATCGACTA
GAAGAGAATGAAGCCATAGACCAGGACCTGCAGGCTTACATCCTGCACCGGATACACAGC
AGCTCAGAGATCCAGAATAACATTTCACTTAATGGCAAAATGGACAATACTACATTTGGC
AAACTCAGTTCTCATCTCAAGACCCTCAGTCAAGGGTCCTATCTATATCTGAAACTTACA
TTTGACCTCATAGAGAAAGGCTATCTAGTGTTAAAGAGCTCTAGCTACAAAGTAGTTCCT
GTTTCGCTCTCAGAGGTTTATTTACTCCAGTGCAATATGAAGTTCCCAACCCAGTCTTCC
TTTGACCGGGTGATGCCTCTCCTGAATGTGGCAGTGGCCTCTCTCCACCCACTGACTGAT
GAGCATATCTTCCAGGCCATCAATGCTGGGAGCATTGAAGGCACACTAGAATGGGAGGAT
TTTCAGCAGAGAATGGAGAACCTCTCCATGTTCCTAATCAAGCGCAGAGACATGACTCGT
ATGTTTGTACATCCTTCTTTTCGAGAATGGCTTATCTGGAGAGAAGAAGGAGAGAAAACC
AAATTTCTCTGTGATCCGAGGAGTGGTCACACGTTACTTGCCTTCTGGTTTTCCCGCCAA
GAGGGAAAACTAAACCGACAGCAGACTATTGAACTGGGACATCACATCCTCAAAGCACAC
ATTTTTAAGGGTTTGAGTAAAAAAGTTGGTGTATCATCCTCCATCCTCCAAGGTCTCTGG
ATCTCTTATAGCACAGAAGGTCTTTCCATGGCACTGGCGTCTTTACGAAATCTCTACACT
CCAAATATAAAGGTCAGCCGACTGCTGATTTTGGGAGGTGCCAATATTAATTACCGGACA
GAGGTTTTAAATAATGCTCCAATTCTATGTGTTCAGTCCCATCTTGGTTACACAGAAATG
GTAGCCCTGCTGCTGGAGTTCGGGGCCAACGTGGATGCCTCTTCTGAAAGTGGCCTGACT
CCCCTGGGATATGCTGCAGCAGCAGGGTACCTGAGCATTGTGGTGCTGCTGTGCAAGAAA
CGGGCCAAGGTGGATCATTTGGATAAGAACGGGCAGTGTGCTTTGGTTCATGCTGCACTC
CGAGGTCATCTGGAGGTTGTCAAGTTTTTGATTCAGTGTGACTGGACGATGGCCGGCCAG
CAGCAAGGAGTATTTAAGAAGAGCCATGCCATCCAACAGGCCCTCATTGCTGCAGCCAGC
ATGGGTTATACTGAGATTGTCTCCTACCTACTTGATCTTCCAGAAAAAGATGAAGAGGAA
GTAGAGCGAGCACAGATCAACAGCTTTGACAGTCTCTGGGGAGAGACAGCCCTAACAGCT
GCAGCCGGAAGGGGCAAACTGGAGGTGTGCCGTTTGCTCTTGGAACAAGGGGCGGCAGTG
GCCCAGCCAAACCGCCGAGGAGCAGTGCCACTATTCAGCACAGTGCGCCAGGGCCACTGG
CAGATTGTTGATCTTTTACTCACCCATGGAGCTGATGTCAACATGGCAGACAAGCAGGGC
CGCACTCCCCTGATGATGGCTGCTTCCGAAGGCCATCTAGGAACCGTGGACTTTCTGCTT
GCACAAGGTGCCTCCATTGCTCTTATGGACAAAGAAGGATTGACAGCCCTCAGCTGGGCT
TGTTTGAAGGGCCATCTCTCAGTAGTACGTTCTCTGGTGGATAACGGAGCTGCCACAGAC
CATGCTGACAAGAATGGCCGTACCCCACTGGATCTGGCAGCTTTCTATGGCGATGCTGAG
GTGGTCCAGTTCCTGGTAGATCATGGGGCCATGATCGAGCACGTTGACTACAGTGGAATG
CGCCCTTTGGATAGGGCAGTGGGGTGCCGGAACACTTCTGTTGTTGTCACTCTTCTGAAG
AAAGGAGCCAAGATAGGTCCAGCCACATGGGCGATGGCCACCTCCAAGCCAGACATCATG
ATCATCCTGTTGAGCAAGCTGATGGAAGAGGGGGACATGTTTTATAAGAAAGGTAAAGTA
AAGGAAGCTGCCCAGCGCTACCAGTACGCCCTGAAGAAGTTCCCTAGAGAAGGGTTTGGT
GAGGACTTGAAAACTTTCCGGGAACTAAAGGTGTCTCTCCTCCTCAACCTCTCTCGGTGT
CGCAGGAAAATGAACGATTTTGGAATGGCGGAGGAATTTGCTACTAAGGCCCTGGAGCTG
AAACCGAAATCTTATGAAGCTTACTATGCGAGAGCAAGGGCAAAACGCAGCAGCAGACAG
TTCGCAGCAGCCTTAGAGGACCTGAACGAGGCCATCAAGCTGTGTCCCAACAACCGTGAG
ATCCAGAGACTTCTGCTGAGAGTGGAAGAAGAGTGTAGACAGATGCAGCAGCCACAGCAG
CCACCGCCGCCACCGCAGCCTCAGCAGCAGTTGCCGGAAGAAGCAGAACCTGAGCCACAG
CATGAAGACATATACTCTGTACAGGATATATTCGAGGAGGAGTACCTGGAACAGGATGTT
GAAAATGTTTCCATTGGCCTCCAGACAGAGGCCCGGCCCAGCCAGGGGCTCCCGGTCATC
CAGAGCCCACCCTCCTCTCCCCCGCATCGGGACTCAGCCTACATCTCCAGCTCACCTCTT
GGCTCTCATCAGGTTTTTGACTTCCGGTCCAGTAGTTCTGTAGGCTCTCCCACTAGACAG
ACCTATCAGTCCACCTCACCTGCCCTTTCTCCAACTCATCAGAACTCACATTACAGGCCT
AGCCCACCACACACTTCCCCGGCTCATCAGGGAGGATCTTACCGTTTCAGCCCCCCTCCT
GTGGGAGGACAGGGCAAAGAATACCCAAGCCCTCCCCCTTCCCCTCTCCGGAGAGGCCCT
CAGTATCGGGCCAGCCCTCCAGCTGAAAGTATGAGTGTCTATAGATCCCAGTCTGGTTCA
CCCGTGCGCTATCAGCAGGAAACAAGCGTCAGTCAGCTTCCTGGCAGACCCAAATCTCCA
TTATCCAAAATGGCCCAGCGGCCCTACCAGATGCCTCAGCTCCCTGTGGCAGTTCCCCAG
CAAGGGCTCAGGCTACAGCCTGCCAAGGCCCAGATTGTGAGAAGTAACCAGCCCAGCCCA
GCCGTCCATTCAAGCACCGTCATCCCCACAGGAGCCTATGGCCAAGTAGCCCATTCAATG
GCCAGTAAATACCAGTCTTCACAAGGAGACATAGGAGTCAGCCAGAGCCGGTTGGTTTAT
CAAGGGTCAATTGGGGGAATCGTAGGGGATGGAAGGCCGGTGCAGCATGTCCAAGCCAGC
CTGAGTGCAGGCGCCATCTGTCAGCATGGAGGATTGACCAAAGAGGATCTTCCACAGCGA
CCTTCCTCAGCATACCGAGGTGGCGTGAGATACAGCCAGACACCACAGATCGGACGCAGC
CAGTCAGCATCCTATTACCCAGTCTGTCACTCAAAACTAGATCTGGAGCGCTCCTCCAGC
CAACTAGGTTCCCCTGATGTGTCGCATTTAATCAGAAGACCTATCAGTGTCAACCCTAAC
GAAATCAAACCGCACCCGCCAACTCCCAGGCCGTTGCTGCATTCCCAAAGTGTAGGCCTT
CGCTTCTCTCCATCTAGCAATAGTATCTCCTCCACCTCCAACCTAACTCCGACCTTCCGG
CCATCTTCTTCCATCCAGCAAATGGAGATCCCACTGAAACCTGCATATGAGAGGTCATGT
GACGAGCTGTCGCCAGTGTCTCCAACTCAAGGAGGTTACCCCAGTGAGCCCACCCGATCC
AGGACCACACCATTCATGGGGATCATAGATAAAACAGCACGGACTCAGCAGTACCCCCAC
CTCCACCAGCAGAATCGGACCTGGGCAGTGTCATCTGTGGACACCGTCCTCAGTCCCACG
TCTCCAGGCAACCTGCCTCAGCCTGAGTCCTTCAGTCCACCATCATCCATCAGCAACATT
GCCTTTTATAACAAAACCAACAATGCACAGAATGGCCATTTGCTGGAGGACGATTATTAC
AGCCCCCATGGGATGCTGGCTAACGGGTCTCGTGGAGACCTCTTGGAGCGAGTCAGCCAG
GCCTCCTCCTATCCCGACGTGAAGGTAGCTCGGACTCTACCTGTGGCTCAGGCATACCAG
GACAACCTGTACAGGCAGCTGTCCCGAGACTCTCGGCAAGGGCAGACATCCCCTATCAAA
CCAAAGAGACCGTTCGTGGAGTCTAATGTT
Protein sequence predicted based on nucleotide sequence
MFRNSLKMLLTGGKSSRKNRSSDGGSEEPPDRRQSSVDSRQSRSGQGGISTESDCAFEPD YAVPPLPVSEGDAEQELGPPPSVDEAANTLMTRLGFLLGEKVTEVQPGDQYSMEVQDENQ TSAITQRISPCSTLTSSTASPPASSPCSTLPPISTNATAKDCSYGAVTSPTSTLESRDSG IIATLTSYSENVERTKYAGESSKELGSGGNIKPWQSQKSSMDSCLYRVDENMTASTYSLN KIPERNLETVLSQSVQSIPLYLMPRPNSVAATSSAHLEDLAYLDEQRHTPLRTSLRMPRQ SMGGARTQQDLRVRFAPYRPPDISLKPLLFEVPSITTESVFVGRDWVFHEIDAQLQSSNA SVNQGVVIVGNIGFGKTAIISRLVALSCHGTRMRQIASDSPHASPKHVDANRELPLTQPP SAHSSITSGSCPGTPEMRRRQEEAMRRLASQVVAYHYCQADNAYTCLVPEFVHNVAALLC RSPQLTAYREQLLREPHLQSMLSLRSCVQDPMASFRRGVLEPLENLHKERKIPDEDFIIL IDGLNEAEFHKPDYGDTIVSFLSKMIGKFPSWLKLIVTVRTSLQEITKLLPFHRIFLDRL EENEAIDQDLQAYILHRIHSSSEIQNNISLNGKMDNTTFGKLSSHLKTLSQGSYLYLKLT FDLIEKGYLVLKSSSYKVVPVSLSEVYLLQCNMKFPTQSSFDRVMPLLNVAVASLHPLTD EHIFQAINAGSIEGTLEWEDFQQRMENLSMFLIKRRDMTRMFVHPSFREWLIWREEGEKT KFLCDPRSGHTLLAFWFSRQEGKLNRQQTIELGHHILKAHIFKGLSKKVGVSSSILQGLW ISYSTEGLSMALASLRNLYTPNIKVSRLLILGGANINYRTEVLNNAPILCVQSHLGYTEM VALLLEFGANVDASSESGLTPLGYAAAAGYLSIVVLLCKKRAKVDHLDKNGQCALVHAAL RGHLEVVKFLIQCDWTMAGQQQGVFKKSHAIQQALIAAASMGYTEIVSYLLDLPEKDEEE VERAQINSFDSLWGETALTAAAGRGKLEVCRLLLEQGAAVAQPNRRGAVPLFSTVRQGHW QIVDLLLTHGADVNMADKQGRTPLMMAASEGHLGTVDFLLAQGASIALMDKEGLTALSWA CLKGHLSVVRSLVDNGAATDHADKNGRTPLDLAAFYGDAEVVQFLVDHGAMIEHVDYSGM RPLDRAVGCRNTSVVVTLLKKGAKIGPATWAMATSKPDIMIILLSKLMEEGDMFYKKGKV KEAAQRYQYALKKFPREGFGEDLKTFRELKVSLLLNLSRCRRKMNDFGMAEEFATKALEL KPKSYEAYYARARAKRSSRQFAAALEDLNEAIKLCPNNREIQRLLLRVEEECRQMQQPQQ PPPPPQPQQQLPEEAEPEPQHEDIYSVQDIFEEEYLEQDVENVSIGLQTEARPSQGLPVI QSPPSSPPHRDSAYISSSPLGSHQVFDFRSSSSVGSPTRQTYQSTSPALSPTHQNSHYRP SPPHTSPAHQGGSYRFSPPPVGGQGKEYPSPPPSPLRRGPQYRASPPAESMSVYRSQSGS PVRYQQETSVSQLPGRPKSPLSKMAQRPYQMPQLPVAVPQQGLRLQPAKAQIVRSNQPSP AVHSSTVIPTGAYGQVAHSMASKYQSSQGDIGVSQSRLVYQGSIGGIVGDGRPVQHVQAS LSAGAICQHGGLTKEDLPQRPSSAYRGGVRYSQTPQIGRSQSASYYPVCHSKLDLERSSS QLGSPDVSHLIRRPISVNPNEIKPHPPTPRPLLHSQSVGLRFSPSSNSISSTSNLTPTFR PSSSIQQMEIPLKPAYERSCDELSPVSPTQGGYPSEPTRSRTTPFMGIIDKTARTQQYPH LHQQNRTWAVSSVDTVLSPTSPGNLPQPESFSPPSSISNIAFYNKTNNAQNGHLLEDDYY SPHGMLANGSRGDLLERVSQASSYPDVKVARTLPVAQAYQDNLYRQLSRDSRQGQTSPIK PKRPFVESNV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
