MYH8 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MYH1
Gene Name:  myosin, heavy chain 1, skeletal muscle, adult
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069080)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399170
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 5814nts
Open reading frame: 1 to 5814

ATGAGTGCGAGCTCAGACGCTGAGATGGCTGTTTTTGGCGAAGCTGCTCCCTACCTTCGA
AAATCAGAAAAGGAGCGGATTGAGGCCCAAAACAAGCCGTTTGATGCTAAAACATCTGTC
TTTGTGGCGGAGCCCAAGGAATCCTATGTGAAGAGCACTATACAAAGCAAAGAAGGAGGG
AAAGTAACCGTAAAGACTGAAGGTGGAGCAACTCTAACTGTCAGGGAAGACCAAGTCTTC
CCTATGAACCCTCCGAAATATGACAAAATTGAGGACATGGCCATGATGACTCATCTACAC
GAGCCTGGAGTGCTGTACAACCTCAAAGAGCGCTATGCAGCCTGGATGATCTACACCTAC
TCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAAGCCCGAG
GTGGTGGCTGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATC
TCTGACAATGCCTATCAGTTCATGTTGACTGATCGAGAGAATCAGTCCATCCTGATCACC
GGAGAATCTGGTGCCGGAAAGACTGTGAACACCAAGCGTGTCATCCAATACTTTGCAACA
ATTGCAGTTACTGGAGAGAAGAAGAAGGATGAATCTGGCAAAATGCAGGGGACTCTGGAA
GATCAAATCATCAGCGCCAATCCCCTACTGGAGGCCTTTGGCAATGCCAAAACTGTGAGG
AATGACAACTCCTCTCGCTTTGGTAAATTCATTAGAATCCACTTTGGTACTACAGGGAAG
CTGGCATCTGCTGATATAGAAACATATCTTTTAGAAAAGTCCAGAGTTACTTTCCAGCTA
AAGGCGGAAAGAAGCTACCATATTTTTTATCAGATCACTTCCAATAAGAAGCCAGATCTA
ATTGAAATGCTCCTGATCACCACCAACCCATATGACTATGCCTTCGTCAGTCAGGGGGAG
ATCACAGTTCCCAGTATTGATGACCAAGAAGAGTTGATGGCCACTGATAGTGCCATTGAC
ATCCTGGGCTTCACTCCTGAAGAGAAAGTGTCCATCTATAAACTCACAGGGGCTGTGATG
CATTATGGGAACATGAAATTCAAGCAAAAGCAGCGTGAGGAGCAAGCTGAGCCAGATGGC
ACAGAAGTCGCTGACAAGGCAGCCTATCTCCAGAGTCTGAACTCTGCAGACCTACTCAAA
GCCCTCTGCTACCCTAGGGTCAAGGTTGGCAATGAGTATGTCACCAAAGGCCAGACTGTG
CAGCAGGTGTACAATGCGGTGGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTG
TGGATGGTCACCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATC
GGGGTCTTGGACATTGCTGGCTTTGAAATCTTTGATTTTAACAGCCTGGAGCAGCTGTGC
ATCAACTTCACCAACGAGAAACTGCAACAGTTTTTCAACCACCACATGTTTGTGCTAGAG
CAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCATTGACTTTGGGATGGACCTG
GCTGCCTGCATTGAGCTCATTGAGAAGCCACTGGGCATCTTCTCCATCCTGGAAGAGGAG
TGCATGTTCCCTAAGGCAACGGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTG
GGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAGGCTGAGGCCCACTTC
TCTCTGATTCACTATGCTGGCACTGTGGACTACAACATTACTGGCTGGCTGGACAAAAAT
AAGGACCCCCTGAATGATACTGTGGTTGGGCTGTACCAGAAGTCTGCAATGAAGACTCTA
GCCAGTCTCTTTTCCACGTATGCTAGTGCTGAAGCAGATAGCAGCGCGAAGAAAGGTGCT
AAGAAAAAGGGCTCTTCTTTCCAGACTGTGTCTGCCCTTTTCAGGGAAAATTTAAATAAA
TTGATGACGAATCTGAGGAGCACACACCCTCACTTCGTACGGTGTATCATTCCCAATGAA
ACCAAAACTCCTGGGGCAATGGAACATGAACTTGTGTTGCACCAGCTGAGGTGTAATGGT
GTGCTGGAAGGCATCCGCATCTGTAGGAAAGGATTCCCAAGCAGAATCTTATATGGTGAT
TTCAAACAAAGATACAAGGTTTTAAATGCAAGTGCTATTCCAGAGGGACAGTTCATTGAC
AGCAAGAAGGCTTCTGAGAAACTTCTTGCATCTATTGATATTGATCATACTCAATATAAA
TTTGGACATACCAAGGTTTTCTTCAAAGCTGGACTTCTGGGTCTTCTGGAAGAAATGAGA
GATGAAAAATTAGCCCAAATTATAACAAGAACACAAGCTGTCTGTAGGGGATTCCTAATG
AGGGTAGAATATCAGAAGATGTTGCAAAGGAGAGAAGCACTTTTCTGCATCCAGTATAAT
GTCCGTGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTTTTCAAGATTAAG
CCCCTCCTCAAGAGTGCAGAGACCGAGAAAGAGATGGCCACCATGAAGGAAGAATTCCAG
AAAACCAAAGATGAACTCGCCAAGTCAGAGGCAAAACGGAAGGAGCTAGAGGAAAAAATG
GTCACTCTCTTAAAAGAGAAAAATGACCTGCAACTCCAGGTTCAATCTGAAGCAGATAGC
TTGGCTGATGCAGAGGAAAGGTGTGAGCAACTGATTAAAAACAAAATCCAACTTGAGGCC
AAAATCAAAGAGGTGACTGAAAGAGCTGAGGAGGAGGAAGAGATCAATGCTGAGCTGACA
GCCAAGAAGAGAAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGACATTGATGACCTT
GAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACGGAGAACAAGGTGAAAAAT
CTTACAGAAGAGATGGCAGGCCTGGATGAAACCATTGCAAAACTGTCCAAGGAGAAGAAG
GCTCTCCAAGAGACCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTC
AACATCCTGACCAAAGCTAAAACCAAGCTAGAACAGCAAGTGGATGATCTTGAAGGGTCT
CTGGAACAAGAAAAGAAGCTTCGAATGGATCTAGAAAGAGCAAAGCGGAAACTGGAGGGT
GACCTCAAATTGGCCCAAGAATCCACAATGGATATGGAAAATGACAAACAGCAACTTGAT
GAAAAGCTTGAAAAGAAAGAATTTGAAATCAGCAATTTGATAAGCAAAATTGAAGATGAG
CAAGCTGTAGAAATTCAACTACAGAAGAAGATCAAAGAGTTGCAGGCCCGCATTGAGGAG
CTGGGGGAAGAAATCGAGGCAGAGAGGGCGTCCCGAGCCAAAGCGGAGAAGCAGCGCTCT
GACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGTGGGGCAACT
TCTGCTCAGGTGGAATTGAACAAGAAGCGGGAGGCTGAGTTTCAGAAACTGCGCAGGGAC
CTGGAGGAGGCCACCCTGCAGCATGAAGCTATGGTGGCTGCTCTTCGGAAGAAGCACGCA
GACAGTATGGCTGAGCTTGGGGAGCAGATTGACAACTTGCAGCGGGTCAAACAGAAGCTG
GAGAAGGAGAAGAGTGAGCTGAAGATGGAGACTGATGACCTCAGCAGTAACGCAGAGGCC
ATTTCCAAAGCCAAGGGAAACCTTGAAAAGATGTGCCGCTCTCTAGAAGATCAAGTGAGT
GAGCTTAAGACCAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGA
GCGCGCCTGCAGACAGAAGCGGGTGAATATTCTCGACAATTAGATGAGAAAGATGCTTTA
GTCTCTCAGCTTTCAAGGAGCAAGCAAGCATCTACTCAGCAGATTGAAGAGCTGAAACAT
CAACTAGAGGAAGAAACTAAAGCCAAGAACGCCCTGGCACACGCCCTGCAGTCCTCCCGC
CATGACTGCGACCTGCTGCGGGAACAGTATGAGGAAGAGCAGGAAGGCAAAGCTGAGCTG
CAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGAACCAAATACGAGACG
GATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAAAAGTTGGCCCAGCGCCTG
CAAGAAGCTGAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTTGAGAAGACG
AAGCAGCGGCTCCAGAATGAAGTTGAAGACCTCATGCTTGATGTGGAAAGGTCTAATGCA
GCCTGTGCAGCCCTTGATAAGAAGCAAAGGAACTTTGACAAGGTCCTATCAGAATGGAAG
CAGAAGTATGAGGAAACTCAGGCTGAACTTGAGGCCTCCCAGAAGGAGTCACGTTCTCTT
AGCACTGAGCTGTTCAAGGTGAAGAATGTCTATGAGGAATCCCTGGATCAACTCGAAACG
CTAAGAAGAGAAAATAAGAACTTGCAACAGGAGATTTCTGACCTCACTGAGCAGATTGCA
GAGGGAGGAAAGCAAATTCATGAATTGGAGAAAATAAAGAAGCAAGTAGAACAAGAGAAA
TGTGAAATTCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTTGAACATGAAGAAGGAAAG
ATTCTGCGTATCCAGCTTGAGTTAAACCAAGTCAAGTCTGAAGTTGATAGAAAAATCGCA
GAAAAGGATGAGGAAATTGACCAGCTGAAGAGAAACCACACTAGAGTCGTGGAGACAATG
CAGAGCACGCTGGATGCAGAGATTAGAAGCAGAAATGATGCTCTGAGAGTCAAGAAGAAA
ATGGAAGGAGATCTGAATGAAATGGAAATCCAGCTGAACCATGCCAATCGCTTAGCTGCA
GAGAGTTTAAGGAACTACAGGAACACCCAAGGAATCCTGAAGGAAACCCAGCTCCACCTG
GATGATGCTCTCCGGGGCCAGGAGGACCTCAAGGAACAGCTGGCAATTGTGGAGCGCAGA
GCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGTGGGCCACTCTGGAACAGACAGAGAGA
AGCAGGAAAATCGCCGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTCCAGCTCCTCCAC
ACCCAGAATACCAGTCTCATTAACACCAAGAAGAAATTAGAAAATGACGTTTCCCAACTC
CAAAGTGAAGTGGAAGAAGTAATCCAAGAATCACGCAATGCAGAAGAGAAAGCCAAGAAG
GCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGAAGAAGGAACAGGACACCAGCGCC
CACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCATCGTCTA
GATGAGGCCGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAGGCC
AGGGTACGTGAGCTTGAAGGAGAGGTTGAAAATGAACAGAAACGTAATGCAGAGGCTGTT
AAAGGTTTACGGAAACATGAGCGACGAGTAAAAGAACTCACCTACCAGACTGAAGAAGAT
CGCAAGAATGTTCTCAGGCTGCAGGACTTGGTAGATAAATTACAGGCGAAGGTGAAATCA
TACAAGAGACAAGCTGAGGAGGCTGAGGAACAATCCAATGCTAATCTATCTAAATTCCGC
AAACTCCAGCATGAGCTGGAGGAGGCCGAGGAACGGGCTGACATTGCTGAGTCCCAGGTC
AACAAATTGCGAGTGAAGAGCCGAGAGGTTCACACAAAAATCAGTGCAGAGTAG

Protein sequence predicted based on nucleotide sequence

MSASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAEPKESYVKSTIQSKEGG
KVTVKTEGGATLTVREDQVFPMNPPKYDKIEDMAMMTHLHEPGVLYNLKERYAAWMIYTY
SGLFCVTVNPYKWLPVYKPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILIT
GESGAGKTVNTKRVIQYFATIAVTGEKKKDESGKMQGTLEDQIISANPLLEAFGNAKTVR
NDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPDL
IEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTPEEKVSIYKLTGAVM
HYGNMKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTV
QQVYNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLC
INFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPLGIFSILEEE
CMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKN
KDPLNDTVVGLYQKSAMKTLASLFSTYASAEADSSAKKGAKKKGSSFQTVSALFRENLNK
LMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYGD
FKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMR
DEKLAQIITRTQAVCRGFLMRVEYQKMLQRREALFCIQYNVRAFMNVKHWPWMKLFFKIK
PLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQSEADS
LADAEERCEQLIKNKIQLEAKIKEVTERAEEEEEINAELTAKKRKLEDECSELKKDIDDL
ELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLSKEKKALQETHQQTLDDLQAEEDKV
NILTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDMENDKQQLD
EKLEKKEFEISNLISKIEDEQAVEIQLQKKIKELQARIEELGEEIEAERASRAKAEKQRS
DLSRELEEISERLEEAGGATSAQVELNKKREAEFQKLRRDLEEATLQHEAMVAALRKKHA
DSMAELGEQIDNLQRVKQKLEKEKSELKMETDDLSSNAEAISKAKGNLEKMCRSLEDQVS
ELKTKEEEQQRLINDLTAQRARLQTEAGEYSRQLDEKDALVSQLSRSKQASTQQIEELKH
QLEEETKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYET
DAIQRTEELEEAKKKLAQRLQEAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERSNA
ACAALDKKQRNFDKVLSEWKQKYEETQAELEASQKESRSLSTELFKVKNVYEESLDQLET
LRRENKNLQQEISDLTEQIAEGGKQIHELEKIKKQVEQEKCEIQAALEEAEASLEHEEGK
ILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHTRVVETMQSTLDAEIRSRNDALRVKKK
MEGDLNEMEIQLNHANRLAAESLRNYRNTQGILKETQLHLDDALRGQEDLKEQLAIVERR
ANLLQAEIEELWATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLENDVSQL
QSEVEEVIQESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRL
DEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNAEAVKGLRKHERRVKELTYQTEED
RKNVLRLQDLVDKLQAKVKSYKRQAEEAEEQSNANLSKFRKLQHELEEAEERADIAESQV
NKLRVKSREVHTKISAE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable