Gene:
Gene Symbol:
INF2
Gene Name:
inverted formin, FH2 and WH2 domain containing
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
Plate and tube sources, forward reads, contig'd with each other, and plate and tube sources, reverse reads, contig'd with each other - but none contig'd with the listed insert sequence. BLAST returned four clones, all CARM1 genes: HsCD00832805 (in pAnt7_cGST), HsCD00832436 (in pLenti6.3/V5-DEST), HsCD00832323 (in pJFT7_nHalo_DC(r4)), and HsCD00829721 (in pDONR221).
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399178
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCGGTGAAGGAGGGCGCACAGCGCAAGTGGGCAGCGCTGAAGGAGAAGCTGGGGCCA
CAGGATTCGGACCCCACGGAGGCCAACCTGGAGAGCGCGGACCCCGAGCTGTGCATCCGG
CTGCTCCAGATGCCCTCTGTGGTCAACTACTCCGGCCTGCGCAAGCGCCTGGAGGGCAGC
GACGGCGGCTGGATGGTGCAGTTCCTGGAGCAGAGCGGCCTGGACCTGCTGCTGGAGGCG
CTGGCGCGGCTGTCGGGCCGCGGCGTTGCACGTATCTCCGACGCCCTGCTGCAGCTCACC
TGCGTCAGCTGCGTGCGCGCCGTCATGAACTCGCGGCAGGGCATCGAGTACATCCTCAGC
AACCAGGGCTACGTGCGCCAGCTCTCCCAGGCCCTGGACACATCCAACGTGATGGTGAAG
AAGCAGGTGTTTGAGCTACTGGCTGCCCTGTGCATCTACTCTCCCGAGGGCCACGTGCTG
ACCCTGGACGCCCTGGACCACTACAAGACGGTGTGCAGCCAGCAGTACCGCTTCAGCATT
GTCATGAACGAGCTCTCCGGCAGCGACAACGTGCCCTACGTGGTCACCCTGCTTAGCGTG
ATCAACGCCGTCATCTTGGGCCCCGAGGACCTGCGCGCGCGCACCCAGCTGCGGAACGAG
TTTATCGGGCTGCAGCTGCTGGACGTCCTGGCTCGCCTGCGAGACCTGGAGGATGCCGAC
CTGCTGATCCAGCTGGAGGCTTTCGAGGAGGCTAAGGCCGAGGACGAGGAGGAGCTGCTG
CGAGTCTCTGGCGGGGTCGACATGAGCAGCCACCAGGAGGTCTTTGCCTCCCTGTTCCAC
AAGGTGAGCTGCTCCCCGGTGTCTGCCCAGCTCCTGTCGGTGCTGCAGGGCCTCCTGCAC
CTGGAGCCCACCCTCCGCTCCAGCCAGCTGCTCTGGGAGGCCCTGGAGAGCCTCGTGAAC
CGGGCCGTGCTCCTGGCCAGCGATGCCCAGGAATGCACCCTGGAGGAAGTGGTTGAGCGG
CTCCTGTCTGTCAAGGGGCGACCCAGACCGAGCCCCCTGGTCAAGGCCCATAAAAGCGTC
CAGGCCAACCTAGACCAGAGCCAGAGGGGCAGCTCCCCGCAAAACACTACAACCCCCAAG
CCCAGCGTGGAGGGCCAGCAGCCAGCAGCAGCTGCTGCCTGCGAGCCCGTGGACCACGCC
CAGAGTGAGAGCATCCTGAAAGTTTCGCAGCCCAGAGCCCTGGAGCAGCAGGCGTCCACC
CCACCCCCACCCCCACCCCCACCCCTGCTCCCTGGTTCCAGTGCCGAGCCCCCTCCCCCT
CCCCCACCACCCCCCCTGCCCAGTGTGGGGGCTAAGGCCCTCCCAACAGCACCCCCGCCC
CCACCCCTGCCAGGCCTGGGGGCCATGGCCCCCCCAGCACCTCCTCTACCACCACCCCTG
CCAGGCTCCTGTGAGTTCCTGCCCCCACCACCTCCACCACTCCCGGGCTTGGGATGCCCG
CCCCCACCCCCACCCCTGCTGCCTGGTATGGGCTGGGGCCCTCCTCCACCCCCACCTCCA
CTACTGCCCTGCACCTGCAGCCCCCCCGTGGCGGGAGGCATGGAGGAGGTCATCGTGGCC
CAGGTGGACCATGGCTTGGGCTCAGCATGGGTCCCCAGCCATCGGCGGGTGAACCCACCC
ACACTGCGCATGAAGAAGCTGAACTGGCAGAAGCTGCCATCCAACGTGGCACGTGAGCAC
AACTCTATGTGGGCGTCCCTGAGCAGCCCCGACGCCGAGGCTGTGGAGCCCGACTTCTCC
AGCATCGAGCGACTATTCTCCTTCCCTGCAGCCAAGCCCAAGGAGCCCACCATGGTGGCC
CCCCGGGCCAGGAAGGAGCCCAAGGAGATCACTTTCCTCGATGCCAAGAAGAGCCTGAAC
CTCAACATCTTCCTGAAGCAATTTAAGTGCTCCAACGAGGAGGTCGCTGCTATGATCCGG
GCTGGAGATACCACCAAGTTTGATGTGGAGGTTCTCAAACAACTCCTTAAGCTCCTTCCC
GAGAAGCACGAGATTGAAAACCTGCGGGCATTCACAGAGGAGCGAGCCAAGCTGGCCAGC
GCCGACCACTTCTACCTCCTCCTGCTGGCCATTCCCTGCTACCAGCTGCGAATCGAGTGC
ATGCTGCTGTGTGAGGGCGCGGCCGCCGTGCTGGACATGGTGCGGCCCAAGGCCCAGCTG
GTGCTGGCTGCCTGCGAAAGCCTGCTCACCAGCCGCCAGCTGCCCATCTTCTGCCAGCTG
ATCCTGAGAATTGGGAACTTCCTCAACTACGGCAGCCACACCGGTGACGCCGACGGCTTC
AAGATCAGCACATTGCTGAAGCTCACGGAGACCAAGTCCCAGCAGAACCGCGTGACGCTG
CTGCACCACGTGCTGGAGGAAGCGGAAAAGAGCCACCCCGACCTCCTGCAGCTGCCCCGG
GACCTGGAACAGCCCTCGCAAGCAGCAGGGATCAACCTGGAGATCATCCGCTCAGAGGCC
AGCTCCAACCTGAAGAAGCTTCTGGAGACCGAGCGGAAGGTGTCTGCCTCCGTGGCCGAG
GTCCAGGAGCAGTACACCGAGCGCCTCCAGGCCAGCATCTCGGCCTTCCGGGCACTGGAT
GAGCTGTTTGAGGCCATCGAGCAGAAGCAACGGGAGCTGGCCGACTACCTGTGTGAGGAC
GCCCAGCAGCTGTCCCTGGAGGACACGTTCAGCACCATGAAGGCTTTCCGGGACCTTTTC
CTCCGCGCCCTGAAGGAGAACAAGGACCGGAAGGAGCAGGCGGCGAAGGCAGAGAGGAGG
AAGCAGCAGCTGGCGGAGGAGGAGGCGCGGCGGCCTCGGGGAGAGGACGGGAAGCCTGTC
AGGAAGGGGCCCGGGAAGCAGGAGGAGGTGTGTGTCATCGATGCCCTGCTGGCTGACATC
AGGAAGGGCTTCCAGCTGCGGAAGACAGCCCGGGGCCGCGGGGACACCGACGGGGGCAGC
AAGGCAGCCTCCATGGATCCCCCAAGAGCCACAGAGCCTGTGGCCACCAGTAACCCTGCA
GGAGATCCCGTGGGCAGCACGCGCTGTCCCGCCTCTGAGCCCGGCCTTGATGCTACAACA
GCCAGCGAGTCCCGGGGCTGGGACCTTGTAGACGCCGTGACCCCCGGCCCTCAGCCCACC
CTGGAGCAGTTGGAGGAGGGTGGTCCACGGCCCCTGGAGAGGCGTTCTTCCTGGTATGTG
GATGCCAGCGATGTCCTAACCACTGAGGATCCCCAGTGCCCCCAGCCCTTGGAGGGGGCC
TGGCCGGTGACTCTGGGAGATGCTCAGGCCCTGAAGCCCCTCAAGTTCTCCAGCAACCAG
CCCCCTGCAGCCGGAAGTTCAAGGCAAGATGCCAAGGATCCCACGTCCTTGCTGGGCGTC
CTCCAGGCCGAGGCCGACAGCACAAGTGAGGGGCTGGAGGACGCTGTCCACAGCCGTGGT
GCCAGACCCCCTGCAGCAGGCCCAGGTGGGGATGAGGACGAGGACGAGGAGGACACGGCC
CCAGAGTCCGCACTGGACACATCCCTGGACAAGTCCTTCTCCGAGGATGCGGTGACCGAC
TCCTCGGGGTCGGGCACACTCCCCAGGGCCCGGGGCCGGGCCTCAAAGGGGACCGGGAAG
CGAAGGAAGAAGCGTCCCTCCAGGAGCCAGGAAGAGGTTCCCCCTGATTCTGATGATAAT
AAAACAAAGAAACTGTGTGTGATCCAGTAG
Protein sequence predicted based on nucleotide sequence
MSVKEGAQRKWAALKEKLGPQDSDPTEANLESADPELCIRLLQMPSVVNYSGLRKRLEGS DGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCVSCVRAVMNSRQGIEYILS NQGYVRQLSQALDTSNVMVKKQVFELLAALCIYSPEGHVLTLDALDHYKTVCSQQYRFSI VMNELSGSDNVPYVVTLLSVINAVILGPEDLRARTQLRNEFIGLQLLDVLARLRDLEDAD LLIQLEAFEEAKAEDEEELLRVSGGVDMSSHQEVFASLFHKVSCSPVSAQLLSVLQGLLH LEPTLRSSQLLWEALESLVNRAVLLASDAQECTLEEVVERLLSVKGRPRPSPLVKAHKSV QANLDQSQRGSSPQNTTTPKPSVEGQQPAAAAACEPVDHAQSESILKVSQPRALEQQAST PPPPPPPPLLPGSSAEPPPPPPPPPLPSVGAKALPTAPPPPPLPGLGAMAPPAPPLPPPL PGSCEFLPPPPPPLPGLGCPPPPPPLLPGMGWGPPPPPPPLLPCTCSPPVAGGMEEVIVA QVDHGLGSAWVPSHRRVNPPTLRMKKLNWQKLPSNVAREHNSMWASLSSPDAEAVEPDFS SIERLFSFPAAKPKEPTMVAPRARKEPKEITFLDAKKSLNLNIFLKQFKCSNEEVAAMIR AGDTTKFDVEVLKQLLKLLPEKHEIENLRAFTEERAKLASADHFYLLLLAIPCYQLRIEC MLLCEGAAAVLDMVRPKAQLVLAACESLLTSRQLPIFCQLILRIGNFLNYGSHTGDADGF KISTLLKLTETKSQQNRVTLLHHVLEEAEKSHPDLLQLPRDLEQPSQAAGINLEIIRSEA SSNLKKLLETERKVSASVAEVQEQYTERLQASISAFRALDELFEAIEQKQRELADYLCED AQQLSLEDTFSTMKAFRDLFLRALKENKDRKEQAAKAERRKQQLAEEEARRPRGEDGKPV RKGPGKQEEVCVIDALLADIRKGFQLRKTARGRGDTDGGSKAASMDPPRATEPVATSNPA GDPVGSTRCPASEPGLDATTASESRGWDLVDAVTPGPQPTLEQLEEGGPRPLERRSSWYV DASDVLTTEDPQCPQPLEGAWPVTLGDAQALKPLKFSSNQPPAAGSSRQDAKDPTSLLGV LQAEADSTSEGLEDAVHSRGARPPAAGPGGDEDEDEEDTAPESALDTSLDKSFSEDAVTD SSGSGTLPRARGRASKGTGKRRKKRPSRSQEEVPPDSDDNKTKKLCVIQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
