Gene:
Gene Symbol:
LAMA3
Gene Name:
laminin, alpha 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069096)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399186
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCTCCAGCAGTGAGGCGGTCAGCCTGCAGCATGGGATGGCTGTGGATCTTTGGGGCA
GCCCTGGGGCAGTGTCTGGGCTACAGTTCACAGCAGCAAAGGGTGCCATTTCTTCAGCCT
CCCGGTCAAAGTCAACTGCAAGCGAGTTATGTGGAGTTTAGACCCAGCCAGGGTTGTAGC
CCTGGATACTATCGGGATCATAAAGGCTTGTATACCGGACGGTGTGTTCCCTGCAATTGC
AACGGACATTCAAATCAATGCCAGGATGGCTCAGGCATATGTGTTAACTGTCAGCACAAC
ACCGCGGGAGAGCACTGTGAACGCTGCCAGGAGGGCTACTATGGCAACGCCGTCCACGGA
TCCTGCAGGGCCTGCCCATGTCCTCACACTAACAGCTTTGCCACTGGCTGTGTGGTGAAT
GGGGGAGACGTGCGGTGCTCCTGCAAAGCTGGGTACACAGGAACACAGTGTGAAAGGTGT
GCACCGGGATATTTCGGGAATCCCCAGAAATTCGGAGGTAGCTGCCAACCATGCAGTTGT
AACAGCAATGGCCAGCTGGGCAGCTGTCATCCCCTGACTGGAGACTGCATAAACCAAGAA
CCCAAAGATAGCAGCCCTGCAGAAGAATGTGATGATTGCGACAGCTGTGTGATGACCCTC
CTGAACGACCTGGCCACCATGGGCGAGCAGCTCCGCCTGGTCAAGTCTCAGCTGCAGGGC
CTGAGTGCCAGCGCAGGGCTTCTGGAGCAGATGAGGCACATGGAGACCCAGGCCAAGGAC
CTGAGGAATCAGTTGCTCAACTACCGTTCTGCCATTTCAAATCATGGATCAAAAATAGAA
GGCCTGGAAAGAGAACTGACTGATTTGAATCAAGAATTTGAGACTTTGCAAGAAAAGGCT
CAAGTAAATTCCAGAAAAGCACAAACATTAAACAACAATGTTAATCGGGCAACACAAAGC
GCAAAAGAACTGGATGTGAAGATTAAAAATGTCATCCGGAATGTGCACATTCTTTTAAAG
CAGATCTCTGGGACAGATGGAGAGGGAAACAACGTGCCTTCAGGTGACTTTTCCAGAGAG
TGGGCTGAAGCCCAGCGCATGATGAGGGAACTGCGGAACAGGAACTTTGGAAAGCACCTC
AGAGAAGCAGAAGCTGATAAAAGGGAGTCGCAGCTCTTGCTGAACCGGATAAGGACCTGG
CAGAAAACCCACCAGGGGGAGAACAATGGGCTTGCTAACAGTATCCGGGATTCTTTAAAT
GAATACGAAGCCAAACTCAGTGACCTTCGTGCTCGGCTGCAGGAGGCAGCTGCCCAAGCC
AAGCAGGCAAATGGCTTGAACCAAGAAAACGAGAGAGCTTTGGGAGCCATTCAGAGACAA
GTGAAAGAAATAAATTCCCTGCAGAGTGATTTCACCAAGTATCTAACCACTGCAGACTCA
TCTTTGTTGCAAACCAACATTGCGCTGCAGCTGATGGAGAAAAGCCAGAAGGAATATGAA
AAATTAGCTGCCAGTTTAAATGAAGCAAGACAAGAACTAAGTGACAAAGTAAGAGAACTT
TCCAGATCTGCTGGCAAAACATCCCTTGTGGAGGAGGCAGAAAAGCACGCGCGGTCCTTA
CAAGAGCTGGCAAAGCAGCTGGAAGAGATCAAGAGAAACGCCAGCGGGGATGAGCTGGTG
CGCTGTGCTGTGGATGCCGCCACCGCCTACGAGAACATCCTCAATGCCATCAAAGCGGCC
GAGGACGCAGCCAACAGGGCTGCCAGTGCATCTGAATCTGCCCTCCAGACAGTGATAAAG
GAAGATCTGCCAAGAAAAGCTAAAACCCTGAGTTCCAACAGTGATAAACTGTTAAATGAA
GCCAAGATGACACAAAAGAAGCTAAAGCAAGAAGTCAGTCCAGCTCTCAACAACCTACAG
CAAACCCTGAATATTGTGACAGTTCAGAAAGAAGTGATAGACACCAATCTCACAACTCTC
CGAGATGGTCTTCATGGGATACAGAGAGGTGATATTGATGCTATGATCAGTAGTGCAAAG
AGCATGGTCAGAAAGGCCAACGACATCACAGATGAGGTTCTGGATGGGCTCAACCCCATC
CAGACAGATGTGGAAAGAATTAAGGACACCTATGGGAGGACACAGAACGAAGACTTCAAA
AAGGCTCTGACTGATGCAGATAACTCGGTGAATAAGTTAACCAACAAACTACCTGATCTT
TGGCGCAAGATTGAAAGTATCAACCAACAGCTGTTGCCCTTGGGAAACATCTCTGACAAC
ATGGACAGAATACGAGAACTAATTCAGCAGGCCAGAGATGCTGCCAGTAAGGTTGCTGTC
CCCATGAGGTTCAATGGTAAATCTGGAGTCGAAGTCCGACTGCCAAATGACCTGGAAGAT
TTGAAAGGATATACATCTCTGTCCTTGTTTCTCCAAAGGCCCAACTCAAGAGAAAATGGG
GGTACTGAGAATATGTTTGTGATGTACCTTGGAAATAAAGATGCCTCCCGGGACTACATC
GGCATGGCAGTTGTGGATGGCCAGCTCACCTGTGTCTACAACCTGGGGGACCGTGAGGCT
GAACTCCAAGTGGACCAGATCTTGACCAAGAGTGAGACTAAGGAGGCAGTTATGGATCGG
GTGAAATTTCAGAGAATTTATCAGTTTGCAAGGCTTAATTACACCAAAGGAGCCACATCC
AGTAAACCAGAAACACCCGGAGTCTATGACATGGATGGTAGAAATAGCAATACACTCCTT
AATTTGGATCCTGAAAATGTTGTATTTTATGTTGGAGGTTACCCACCTGATTTTAAACTT
CCCAGTCGACTAAGTTTCCCTCCATACAAAGGTTGTATTGAATTAGATGACCTCAATGAA
AATGTTCTGAGCTTGTACAACTTCAAAAAAACATTCAATCTCAACACAACTGAAGTGGAG
CCTTGTAGAAGGAGGAAGGAAGAGTCAGACAAAAATTATTTTGAAGGTACGGGCTATGCT
CGAGTTCCAACTCAACCACATGCTCCCATCCCAACCTTTGGACAGACAATTCAGACCACC
GTGGATAGAGGCTTGCTGTTCTTTGCAGAAAACGGGGATCGCTTCATATCTCTAAATATA
GAAGATGGCAAGCTCATGGTGAGATACAAACTGAATTCAGAGCTACCAAAAGAGAGAGGA
GTTGGAGACGCCATAAACAACGGCAGAGACCATTCGATTCAGATCAAAATTGGAAAACTC
CAAAAGCGTATGTGGATAAATGTGGACGTTCAAAACACTATAATTGATGGTGAAGTATTT
GATTTCAGCACATATTATCTGGGAGGAATTCCAATTGCAATCAGGGAAAGATTTAACATT
TCTACGCCTGCTTTCCGAGGCTGCATGAAAAATTTGAAGAAAACCAGTGGTGTCGTTAGA
TTGAATGATACTGTGGGAGTAACCAAAAAGTGCTCGGAAGACTGGAAGCTTGTGCGATCT
GCCTCATTCTCCAGAGGAGGACAATTGAGTTTCACTGATTTGGGCTTACCACCTACTGAC
CACCTCCAGGCCTCATTTGGATTTCAGACCTTTCAACCCAGTGGCATATTATTAGATCAT
CAGACATGGACAAGGAACCTGCAGGTCACTCTGGAAGATGGTTACATTGAATTGAGCACC
AGCGATAGCGGCAGCCCAATTTTTAAATCTCCACAGACGTATATGGATGGTTTACTGCAT
TATGTATCTGTAATAAGCGACAACTCTGGACTACGGCTTCTCATCGATGACCAGCTTCTG
AGAAATAGCAAAAGGCTAAAACACATTTCAAGTTCCCGGCAGTCTCTGCGTCTGGGCGGG
AGCAATTTTGAGGGTTGTATTAGCAATGTTTTTGTCCAGAGGTTATCACTGAGTCCTGAA
GTCCTAGATTTGACCAGTAACTCTCTCAAGAGAGATGTGTCCCTGGGAGGCTGCAGTTTA
AACAAACCACCTTTTCTAATGTTGCTTAAAGGTTCTACCAGGTTTAACAAGACCAAGACT
TTTCGTATCAACCAGCTGTTGCAGGACACACCAGTGGCCTCCCCAAGGAGCGTGAAGGTG
TGGCAAGATGCTTGCTCACCACTTCCCAAGACCCAGGCCAATCATGGAGCCCTCCAGTTT
GGGGACATTCCCACCAGCCACTTGCTATTCAAGCTTCCTCAGGAGCTGCTGAAACCCAGG
TCACAGTTTGCTGTGGACATGCAGACAACATCCTCCAGAGGACTGGTGTTTCACACGGGC
ACTAAGAACTCCTTTATGGCTCTTTATCTTTCAAAAGGACGTCTGGTCTTTGCACTGGGG
ACAGATGGGAAAAAATTGAGGATCAAAAGCAAGGAGAAATGCAATGATGGGAAATGGCAC
ACGGTGGTGTTTGGCCATGATGGGGAAAAGGGGCGCTTGGTTGTGGATGGACTGAGGGCC
CGGGAGGGAAGTTTGCCTGGAAACTCCACCATCAGCATCAGAGCGCCAGTTTACCTGGGA
TCACCTCCATCAGGGAAACCAAAGAGCCTCCCCACAAACAGCTTTGTGGGATGCCTGAAG
AACTTTCAGCTGGATTCAAAACCCTTGTATACCCCTTCTTCAAGCTTCGGGGTGTCTTCC
TGCTTGGGTGGTCCTTTGGAGAAAGGCATTTATTTCTCTGAAGAAGGAGGTCATGTCGTC
TTGGCTCACTCTGTATTGTTGGGGCCAGAATTTAAGCTTGTTTTCAGCATCCGCCCAAGA
AGTCTCACTGGGATCCTAATACACATCGGAAGTCAGCCCGGGAAGCACTTATGTGTTTAC
CTGGAGGCAGGAAAGGTCACGGCCTCTATGGACAGTGGGGCAGGTGGGACCTCAACGTCG
GTCACACCAAAGCAGTCTCTGTGTGATGGACAGTGGCACTCGGTGGCAGTCACCATAAAA
CAACACATCCTGCACCTGGAACTGGACACAGACAGTAGCTACACAGCTGGACAGATCCCC
TTCCCACCTGCCAGCACTCAAGAGCCACTACACCTTGGAGGTGCTCCAGCCAATTTGACG
ACACTGAGGATCCCTGTGTGGAAATCATTCTTTGGCTGTCTGAGGAATATTCATGTCAAT
CACATCCCTGTCCCTGTCACTGAAGCCTTGGAAGTCCAGGGGCCTGTCAGTCTGAATGGT
TGTCCTGACCAGTAG
Protein sequence predicted based on nucleotide sequence
MPPAVRRSACSMGWLWIFGAALGQCLGYSSQQQRVPFLQPPGQSQLQASYVEFRPSQGCS PGYYRDHKGLYTGRCVPCNCNGHSNQCQDGSGICVNCQHNTAGEHCERCQEGYYGNAVHG SCRACPCPHTNSFATGCVVNGGDVRCSCKAGYTGTQCERCAPGYFGNPQKFGGSCQPCSC NSNGQLGSCHPLTGDCINQEPKDSSPAEECDDCDSCVMTLLNDLATMGEQLRLVKSQLQG LSASAGLLEQMRHMETQAKDLRNQLLNYRSAISNHGSKIEGLERELTDLNQEFETLQEKA QVNSRKAQTLNNNVNRATQSAKELDVKIKNVIRNVHILLKQISGTDGEGNNVPSGDFSRE WAEAQRMMRELRNRNFGKHLREAEADKRESQLLLNRIRTWQKTHQGENNGLANSIRDSLN EYEAKLSDLRARLQEAAAQAKQANGLNQENERALGAIQRQVKEINSLQSDFTKYLTTADS SLLQTNIALQLMEKSQKEYEKLAASLNEARQELSDKVRELSRSAGKTSLVEEAEKHARSL QELAKQLEEIKRNASGDELVRCAVDAATAYENILNAIKAAEDAANRAASASESALQTVIK EDLPRKAKTLSSNSDKLLNEAKMTQKKLKQEVSPALNNLQQTLNIVTVQKEVIDTNLTTL RDGLHGIQRGDIDAMISSAKSMVRKANDITDEVLDGLNPIQTDVERIKDTYGRTQNEDFK KALTDADNSVNKLTNKLPDLWRKIESINQQLLPLGNISDNMDRIRELIQQARDAASKVAV PMRFNGKSGVEVRLPNDLEDLKGYTSLSLFLQRPNSRENGGTENMFVMYLGNKDASRDYI GMAVVDGQLTCVYNLGDREAELQVDQILTKSETKEAVMDRVKFQRIYQFARLNYTKGATS SKPETPGVYDMDGRNSNTLLNLDPENVVFYVGGYPPDFKLPSRLSFPPYKGCIELDDLNE NVLSLYNFKKTFNLNTTEVEPCRRRKEESDKNYFEGTGYARVPTQPHAPIPTFGQTIQTT VDRGLLFFAENGDRFISLNIEDGKLMVRYKLNSELPKERGVGDAINNGRDHSIQIKIGKL QKRMWINVDVQNTIIDGEVFDFSTYYLGGIPIAIRERFNISTPAFRGCMKNLKKTSGVVR LNDTVGVTKKCSEDWKLVRSASFSRGGQLSFTDLGLPPTDHLQASFGFQTFQPSGILLDH QTWTRNLQVTLEDGYIELSTSDSGSPIFKSPQTYMDGLLHYVSVISDNSGLRLLIDDQLL RNSKRLKHISSSRQSLRLGGSNFEGCISNVFVQRLSLSPEVLDLTSNSLKRDVSLGGCSL NKPPFLMLLKGSTRFNKTKTFRINQLLQDTPVASPRSVKVWQDACSPLPKTQANHGALQF GDIPTSHLLFKLPQELLKPRSQFAVDMQTTSSRGLVFHTGTKNSFMALYLSKGRLVFALG TDGKKLRIKSKEKCNDGKWHTVVFGHDGEKGRLVVDGLRAREGSLPGNSTISIRAPVYLG SPPSGKPKSLPTNSFVGCLKNFQLDSKPLYTPSSSFGVSSCLGGPLEKGIYFSEEGGHVV LAHSVLLGPEFKLVFSIRPRSLTGILIHIGSQPGKHLCVYLEAGKVTASMDSGAGGTSTS VTPKQSLCDGQWHSVAVTIKQHILHLELDTDSSYTAGQIPFPPASTQEPLHLGGAPANLT TLRIPVWKSFFGCLRNIHVNHIPVPVTEALEVQGPVSLNGCPDQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
