Gene:
Gene Symbol:
SIN3B
Gene Name:
SIN3 transcription regulator family member B
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069106)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399196
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGCACGCTGGCGGTGGCAGCGGTGGCAGCGGTGCCGGCGGCCCCGCGGGCCGGGGG
CTGAGCGGCGCCCGCTGGGGTCGCTCGGGCTCCGCAGGCCACGAGAAGCTGCCGGTGCAC
GTAGAAGACGCCCTCACCTATCTGGACCAGGTGAAGATCCGCTTTGGCAGCGACCCTGCC
ACCTACAACGGCTTCCTGGAGATCATGAAGGAGTTCAAAAGCCAGAGCATCGATACTCCT
GGAGTCATCAGACGTGTCTCGCAGCTCTTCCACGAGCACCCTGACCTCATTGTTGGATTC
AACGCTTTTCTTCCCCTCGGATATAGAATAGACATTCCCAAGAATGGCAAGTTAAACATA
CAGTCGCCTCTGACAAGCCAGGAGAATTCGCACAACCACGGGGACGGTGCAGAGGACTTC
AAGCAGCAGGTGCCGTATAAAGAGGACAAACCCCAGGTGCCCCTGGAGTCCGATTCCGTG
GAATTCAACAACGCCATCAGCTATGTGAATAAGATTAAAACCCGCTTCCTAGACCACCCA
GAAATCTACAGGTCATTCCTGGAGATCCTGCACACGTACCAGAAGGAGCAGCTGAACACG
AGGGGCCGGCCATTCCGAGGCATGTCTGAAGAGGAGGTGTTCACCGAGGTGGCCAACCTC
TTCCGGGGCCAGGAGGACCTGCTCTCAGAGTTTGGACAGTTCCTGCCCGAAGCCAAGCGG
TCTCTGTTCACAGGAAACGGGCCGTGCGAGATGCACAGCGTGCAGAAGAACGAGCACGAC
AAGACCCCGGAGCACAGCAGGAAGCGCTCCCGGCCCTCGCTCCTCCGCCCCGTGTCTGCA
CCCGCCAAGAAAAAAATGAAACTTCGTGGTACCAAAGACCTGTCCATCGCTGCAGTGGGG
AAGTACGGGACTCTGCAGGAATTTTCTTTCTTTGACAAGGTCCGCCGGGTGCTGAAGAGC
CAGGAGGTGTATGAAAACTTCCTCCGCTGCATCGCACTCTTCAACCAGGAGCTGGTGTCT
GGCTCTGAGCTCCTGCAGCTCGTCAGCCCATTTCTGGGGAAATTTCCAGAACTCTTTGCA
CAGTTCAAGTCCTTCCTGGGGGTAAAAGAGCTGTCCTTCGCGCCACCCATGAGCGACAGA
TCCGGGGACGGGATAAGCCGGGAAATTGATTATGCATCCTGCAAGCGCATAGGATCCAGC
TACCGGGCACTCCCCAAAACCTACCAGCAGCCCAAGTGCAGTGGGAGGACAGCCATCTGC
AAGGAGCTTGACCATTGGACACTTCTCCAGGGTTCGTGGACAGACGATTACTGCATGTCC
AAGTTCAAGAATACCTGCTGGATTCCAGGATATAGTGCAGGGGTACTGAACGACACCTGG
GTCTCCTTCCCTTCCTGGTCTGAGGACTCCACGTTCGTCAGCTCCAAGAAGACACCGTAC
GAGGAGCAGCTTCACCGCTGTGAGGACGAGCGCTTCGAGTTAGACGTTGTCCTGGAGACG
AACCTGGCCACAATCCGTGTGTTGGAAAGTGTGCAGAAGAAGCTGTCTCGGATGGCGCCG
GAAGACCAGGAGAAGTTCCGGCTGGACGACTCCCTGGGAGGCACGTCGGAGGTGATCCAG
CGCCGTGCCATTTATCGCATCTATGGCGACAAGGCCCCGGAGATCATCGAGAGCCTCAAG
AAGAACCCTGTCACCGCTGTCCCCGTTGTCCTGAAAAGACTGAAGGCCAAGGAAGAGGAG
TGGCGGGAGGCCCAGCAGGGCTTCAACAAGATCTGGCGGGAGCAGTATGAGAAGGCGTAC
CTCAAGTCCCTTGACCACCAGGCTGTGAACTTCAAGCAGAACGACACCAAGGCCCTGCGC
TCCAAGAGCTTGCTCAACGAGATCGAGAGCGTCTACGACGAGCACCAGGAGCAGCACTCG
GAGGGCCGCAGTGCCCCCTCTAGCGAGCCGCACCTCATCTTTGTGTACGAGGACCGGCAG
ATCCTGGAGGACGCAGCAGCGCTCATCAGCTACTACGTGAAGCGGCAGCCGGCCATCCAG
AAGGAGGACCAGGGCACCATCCACCAGCTGCTGCACCAGTTCGTGCCCAGCCTCTTCTTC
TCTCAGCAGCTGGACCTGGGCGCCTCCGAGGAGTCAGCTGATGAGGACCGGGACAGCCCC
CAGGGGCAGACCACAGACCCCAGTGAGCGGAAGAAGCCGGCGCCAGGACCCCACAGTAGC
CCCCCAGAGGAGAAGGGGGCCTTCGGGGATGCCCCGGCCACTGAGCAGCCACCCCTGCCG
CCCCCAGCCCCGCACAAGCCCCTGGACGATGTCTACAGCCTATTTTTTGCCAACAACAAC
TGGTACTTCTTCCTGCGCCTGCACCAGACCCTGTGCTCCAGGCTGCTGAAGATCTACCGC
CAGGCGCAGAAGCAGCTTCTGGAGTATCGGACCGAGAAGGAGCGGGAGAAGCTGCTGTGT
GAGGGCCGCAGGGAGAAGGGCAGCGACCCCGCCATGGAGCTGCGGCTGAAGCAGCCCAGT
GAAGTGGAGCTGGAGGAGTACTACCCGGCCTTCCTGGACATGGTGCGGAGCCTGCTGGAG
GGCAGCATCGACCCCACGCAGTACGAGGACACCCTACGCGAGATGTTCACCATCCATGCC
TACGTGGGCTTCACCATGGACAAGCTGGTGCAGAACATTGCGCGGCAGCTGCACCACCTC
GTGAGCGATGACGTCTGCCTGAAGGTGGTGGAGCTCTACCTGAACGAGAAGAAGCGGGGT
GCCGCTGGTGGGAACCTGTCCTCCCGCTGCGTCCGCGCTGCTAGGGAGACCAGCTACCAG
TGGAAGGCTGAGCGCTGCATGGCCGACGAGAACTGCTTCAAGGTGATGTTCCTGCAGCGC
AAAGGGCAGGTGATCATGACCATCGAGCTCCTGGACACCGAGGAGGCCCAGACGGAGGAC
CCTGTGGAGGTCCAGCACCTGGCTCGGTACGTGGAGCAGTATGTGGGGACCGAGGGCGCG
TCCAGCTCGCCCACTGAGGGCTTCCTCCTGAAACCTGTGTTCCTGCAGAGGAACCTCAAG
AAGTTCCGCCGCCGGTGGCAGAGCGAGCAGGCGCGGGCCCTGCGCGGTGAGGCCAGGAGC
TCCTGGAAGCGGCTGGTGGGCGTGGAGAGCGCCTGCGACGTGGACTGCCGCTTCAAGCTC
AGCACTCACAAGATGGTGTTCATCGTGAACTCCGAGGACTACATGTACCGTCGCGGGACC
CTCTGCCGGGCCAAGCAGGTGCAGCCCCTGGTCCTGCTCCGCCACCACCAGCACTTTGAG
GAGTGGCACAGCCGCTGGCTGGAGGACAATGTGACGGTGGAGGCGGCTAGCCTGGTGCAG
GACTGGCTGATGGGTGAGGAGGACGAGGACATGGTACCCTGCAAGACGCTGTGTGAGACA
GTGCACGTGCACGGCCTGCCCGTGACCCGCTACCGCGTGCAGTACAGCCGCCGCCCGGCC
TCGCCC
Protein sequence predicted based on nucleotide sequence
MAHAGGGSGGSGAGGPAGRGLSGARWGRSGSAGHEKLPVHVEDALTYLDQVKIRFGSDPA TYNGFLEIMKEFKSQSIDTPGVIRRVSQLFHEHPDLIVGFNAFLPLGYRIDIPKNGKLNI QSPLTSQENSHNHGDGAEDFKQQVPYKEDKPQVPLESDSVEFNNAISYVNKIKTRFLDHP EIYRSFLEILHTYQKEQLNTRGRPFRGMSEEEVFTEVANLFRGQEDLLSEFGQFLPEAKR SLFTGNGPCEMHSVQKNEHDKTPEHSRKRSRPSLLRPVSAPAKKKMKLRGTKDLSIAAVG KYGTLQEFSFFDKVRRVLKSQEVYENFLRCIALFNQELVSGSELLQLVSPFLGKFPELFA QFKSFLGVKELSFAPPMSDRSGDGISREIDYASCKRIGSSYRALPKTYQQPKCSGRTAIC KELDHWTLLQGSWTDDYCMSKFKNTCWIPGYSAGVLNDTWVSFPSWSEDSTFVSSKKTPY EEQLHRCEDERFELDVVLETNLATIRVLESVQKKLSRMAPEDQEKFRLDDSLGGTSEVIQ RRAIYRIYGDKAPEIIESLKKNPVTAVPVVLKRLKAKEEEWREAQQGFNKIWREQYEKAY LKSLDHQAVNFKQNDTKALRSKSLLNEIESVYDEHQEQHSEGRSAPSSEPHLIFVYEDRQ ILEDAAALISYYVKRQPAIQKEDQGTIHQLLHQFVPSLFFSQQLDLGASEESADEDRDSP QGQTTDPSERKKPAPGPHSSPPEEKGAFGDAPATEQPPLPPPAPHKPLDDVYSLFFANNN WYFFLRLHQTLCSRLLKIYRQAQKQLLEYRTEKEREKLLCEGRREKGSDPAMELRLKQPS EVELEEYYPAFLDMVRSLLEGSIDPTQYEDTLREMFTIHAYVGFTMDKLVQNIARQLHHL VSDDVCLKVVELYLNEKKRGAAGGNLSSRCVRAARETSYQWKAERCMADENCFKVMFLQR KGQVIMTIELLDTEEAQTEDPVEVQHLARYVEQYVGTEGASSSPTEGFLLKPVFLQRNLK KFRRRWQSEQARALRGEARSSWKRLVGVESACDVDCRFKLSTHKMVFIVNSEDYMYRRGT LCRAKQVQPLVLLRHHQHFEEWHSRWLEDNVTVEAASLVQDWLMGEEDEDMVPCKTLCET VHVHGLPVTRYRVQYSRRPASP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
