Gene:
Gene Symbol:
PRDM2
Gene Name:
PR domain containing 2, with ZNF domain
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069108)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399198
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATCAGAACACTACTGAGCCTGTGGCGGCCACCGAGACCCTGGCTGAGGTACCCGAA
CATGTGCTGCGAGGACTTCCGGAGGAAGTGAGGCTTTTCCCTTCTGCTGTTGACAAGACC
CGGATTGGTGTCTGGGCCACTAAACCAATTTTAAAAGGCAAAAAATTTGGGCCATTTGTT
GGTGATAAGAAAAAAAGATCTCAGGTTAAGAATAATGTATACATGTGGGAGGTGTATTAC
CCAAATTTGGGATGGATGTGCATTGATGCCACTGATCCAGAGAAGGGAAACTGGCTGCGA
TATGTGAATTGGGCTTGCTCAGGAGAAGAGCAAAATTTATTCCCACTGGAAATCAACAGA
GCCATTTACTATAAAACTTTAAAGCCAATCGCGCCGGGCGAGGAGCTCCTGGTCTGGTAC
AATGGGGAAGACAACCCTGAGATAGCAGCTGCGATTGAGGAAGAGCGAGCCAGCGCCCGG
AGCAAGCGGAGCTCCCCCAAGAGCCGGAAAGGGAAGAAAAAATCCCAGGAAAATAAAAAC
AAAGGAAACAAAATCCAAGACATACAACTGAAGACAAGTGAGCCAGATTTCACCTCTGCA
AATATGAGAGATTCTGCAGAAGGTCCTAAAGAAGACGAAGAGAAGCCTTCAGCCTCAGCA
CTTGAGCAGCCGGCCACCCTCCAGGAGGTGGCCAGTCAGGAGGTGCCTCCAGAACTAGCA
ACCCCTGCCCCTGCCTGGGAGCCACAGCCAGAACCAGACGAGCGATTAGAAGCGGCAGCT
TGTGAGGTGAATGATTTGGGGGAAGAGGAGGAGGAGGAAGAGGAGGAGGATGAAGAAGAA
GAAGAAGATGATGATGATGATGAGTTGGAAGACGAGGGGGAAGAAGAAGCCAGCATGCCA
AATGAAAATTCTGTGAAAGAGCCAGAAATACGGTGTGATGAGAAGCCAGAAGATTTATTA
GAGGAACCAAAAACAACTTCAGAAGAAACTCTTGAAGACTGCTCAGAGGTAACACCTGCC
ATGCAAATCCCCAGAACTAAAGAAGAGGCCAATGGTGATGTATTTGAAACGTTTATGTTT
CCGTGTCAACATTGTGAAAGGAAGTTTACAACCAAACAGGGGCTTGAGCGTCACATGCAT
ATCCATATATCCACCGTCAATCATGCTTTCAAATGCAAGTACTGTGGGAAAGCCTTTGGC
ACACAGATTAACCGGCGGCGACATGAGCGGCGCCATGAAGCAGGGTTAAAGCGGAAACCC
AGCCAAACACTACAGCCGTCAGAGGATCTGGCTGATGGCAAAGCATCTGGAGAAAACGTT
GCTTCAAAAGATGATTCGAGTCCTCCCAGTCTTGGGCCAGACTGTCTGATCATGAATTCA
GAGAAGGCTTCCCAAGACACAATAAATTCTTCTGTCGTAGAAGAGAATGGGGAAGTTAAA
GAACTTCATCCGTGCAAATATTGTAAAAAGGTTTTTGGAACTCATACTAATATGAGACGG
CATCAGCGTAGAGTTCACGAACGTCATCTGATTCCCAAAGGTGTACGGCGAAAAGGAGGC
CTTGAAGAGCCCCAGCCTCCAGCAGAACAGGCCCAGGCCACCCAGAACGTGTATGTACCA
AGCACAGAGCCGGAGGAGGAAGGGGAAGCAGATGATGTGTACATCATGGACATTTCTAGC
AATATCTCTGAAAACTTAAATTACTATATTGATGGTAAAATTCAAACTAATAACAACACT
AGTAACTGTGATGTGATTGAGATGGAGTCTGCTTCGGCAGATTTGTATGGTATAAATTGT
CTGCTCACTCCAGTTACAGTGGAAATTACTCAAAATATAAAGACCACACAGGTCCCTGTA
ACAGAAGATCTTCCTAAAGAGCCTTTGGGCAGCACAAATAGTGAGGCCAAGAAGCGGAGA
ACTGCGAGCCCACCTGCACTGCCCAAAATTAAGGCCGAAACAGACTCTGACCCCATGGTC
CCCTCTTGCTCTTTAAGTCTTCCTCTTAGCATATCAACAACAGAGGCAGTGTCTTTCCAC
AAAGAGAAAAGTGTTTATTTGTCATCAAAGCTCAAACAACTTCTTCAAACCCAAGATAAA
CTAACTCCTGCAGGGATTTCAGCAACTGAAATAGCTAAATTAGGTCCTGTTTGTGTGTCT
GCTCCTGCATCAATGTTGCCTGTGACCTCAAGTAGGTTTAAGAGGCGGACCAGCTCTCCT
CCCAGTTCTCCACAGCACAGTCCTGCCCTTCGAGACTTTGGAAAGCCAAGTGATGGGAAA
GCAGCATGGACCGATGCCGGGCTGACTTCCAAAAAATCCAAATTAGAAAGTCACAGCGAC
TCACCAGCATGGAGTTTGTCTGGGAGAGATGAGAGAGAAACTGTGAGCCCTCCATGCTTT
GATGAATATAAAATGTCTAAAGAGTGGACAGCTAGTTCTGCTTTTAGCAGTGTGTGCAAC
CAGCAGCCACTGGATTTATCCAGCGGTGTCAAACAGAAGGCTGAGGGTACAGGCAAGACT
CCAGTCCAGTGGGAATCTGTCTTAGATCTCAGTGTGCATAAAAAGCATTGTAGTGACTCT
GAAGGCAAGGAATTCAAAGAAAGTCATTCAGTGCAGCCTACGTGTAGTGCTGTAAAGAAA
AGGAAACCAACCACCTGCATGCTGCAGAAGGTTCTTCTCAATGAATATAATGGCATCGAT
TTACCTGTAGAAAACCCTGCAGATGGGACCAGGAGCCCAAGTCCTTGTAAATCCCTAGAA
GCTCAGCCAGATCCTGACCTCGGTCCGGGCTCTGGTTTCCCTGCCCCTACTGTTGAGTCC
ACACCTGATGTTTGTCCTTCATCACCTGCCCTGCAGACACCCTCCCTTTCATCCGGTCAG
CTGCCTCCTCTCTTGATCCCCACAGATCCCTCTTCCCCTCCACCCTGTCCCCCGGTATTA
ACTGTTGCCACTCCGCCCCCTCCCCTCCTTCCTACCGTACCTCTTCCAGCCCCCTCTTCC
AGTGCATCTCCACACCCATGCCCCTCTCCACTCTCAAATGCCACCGCACAGTCCCCACTT
CCAATTCTGTCCCCAACAGTGTCCCCCTCTCCCTCTCCCATTCCTCCCGTGGAGCCCCTG
ATGTCTGCCGCCTCACCCGGGCCTCCAACACTTTCTTCTTCCTCCTCTTCATCTTCCTCC
TCCTCTTCGTTTTCTTCTTCATCTTCCTCCTCTTCTCCTTCTCCACCTCCTCTCTCCGCA
ATATCATCTGTTGTTTCCTCTGGTGATAATCTGGAGGCTTCTCTCCCCATGATATCTTTC
AAACAGGAGGAATTAGAGAATGAAGGTCTGAAACCCAGGGAAGAGCCCCAGTCTGCTGCT
GAACAGGATGTTGTTGTTCAGGAAACATTCAACAAAAACTTTGTTTGCAACGTCTGTGAA
TCACCTTTTCTTTCCATTAAAGATCTAACCAAACATTTATCTATTCATGCTGAAGAATGG
CCCTTCAAATGTGAATTTTGTGTGCAGCTTTTTAAGGATAAAACGGACTTGTCAGAACAT
CGCTTTTTGCTTCATGGAGTTGGGAATATCTTTGTGTGTTCTGTTTGTAAAAAAGAATTT
GCTTTTTTGTGCAATTTGCAGCAGCACCAGCGAGATCTCCACCCAGATAAGGTGTGCACA
CATCACGAGTTTGAAAGCGGGACTCTGAGGCCCCAGAACTTTACAGATCCCAGCAAGGCC
CATGTAGAGCATATGCAGAGCTTGCCAGAAGATCCTTTAGAAACTTCTAAAGAAGAAGAG
GAGTTAAATGATTCCTCTGAAGAGCTTTACACGACTATAAAAATAATGGCTTCTGGAATA
AAGACAAAAGATCCAGATGTTCGATTGGGCCTCAATCAGCATTACCCAAGCTTTAAACCA
CCTCCATTTCAGTACCATCACCGTAACCCCATGGGGATTGGTGTGACAGCCACAAATTTC
ACTACACACAATATTCCACAGACTTTCACTACCGCCATTCGCTGCACAAAGTGTGGAAAA
GGTGTCGACAATATGCCGGAGTTGCACAAACATATCCTGGCTTGTGCTTCTGCAAGTGAC
AAGAAGAGGTACACGCCTAAGAAAAACCCAGTACCATTAAAACAAACTGTGCAACCCAAA
AATGGCGTGGTGGTTTTAGATAACTCTGGGAAAAATGCCTTCCGACGAATGGGACAGCCC
AAAAGGCTTAACTTTAGTGTTGAGCTCAGCAAAATGTCGTCGAATAAGCTCAAATTAAAT
GCATTGAAGAAAAAAAATCAGCTAGTACAGAAAGCAATTCTTCAGAAAAACAAATCTGCA
AAGCAGAAGGCCGACTTGAAAAATGCTTGTGAGTCATCCTCTCACATCTGCCCTTACTGT
AATCGAGAGTTCACTTACATTGGAAGCCTGAATAAACACGCCGCCTTCAGCTGTCCCAAA
AAACCCCTTTCTCCTCCCAAAAAAAAAGTTTCTCATTCATCTAAGAAAGGTGGACACTCA
TCACCTGCAAGTAGTGACAAAAACAGTAACAGCAACCACCGCAGACGGACAGCGGATGCG
GAGATTAAAATGCAAAGCATGCAGACTCCGTTGGGCAAGACCAGAGCCCGCAGCTCAGGC
CCCACCCAAGTCCCACTTCCCTCCTCATCCTTCAGGTCCAAGCAGAACGTCAAGTTTGCA
GCTTCGGTGAAATCCAAAAAACCAAGCTCCTCCTCTTTAAGGAACTCCAGCCCGATAAGA
ATGGCCAAAATAACTCATGTTGAGGGGAAAAAACCTAAAGCTGTGGCCAAGAATCATTCT
GCTCAGCTTTCCAGCAAAACATCACGGAGCCTGCACGTGAGGGTACAGAAAAGCAAAGCT
GTTTTACAAAGCAAATCCACCTTGGCGAGTAAGAAAAGAACAGACCGGTTCAATATAAAA
TCTAGAGAGCGGAGTGGGGGGCCAGTCACCCGGAGCCTTCAGCTGGCAGCTGCTGCTGAC
TTGAGTGAGAACAAGAGAGAGGACGGCAGCGCCAAGCAGGAGCTGAAGGACTTCAGGAAC
TTCCTGTAG
Protein sequence predicted based on nucleotide sequence
MNQNTTEPVAATETLAEVPEHVLRGLPEEVRLFPSAVDKTRIGVWATKPILKGKKFGPFV GDKKKRSQVKNNVYMWEVYYPNLGWMCIDATDPEKGNWLRYVNWACSGEEQNLFPLEINR AIYYKTLKPIAPGEELLVWYNGEDNPEIAAAIEEERASARSKRSSPKSRKGKKKSQENKN KGNKIQDIQLKTSEPDFTSANMRDSAEGPKEDEEKPSASALEQPATLQEVASQEVPPELA TPAPAWEPQPEPDERLEAAACEVNDLGEEEEEEEEEDEEEEEDDDDDELEDEGEEEASMP NENSVKEPEIRCDEKPEDLLEEPKTTSEETLEDCSEVTPAMQIPRTKEEANGDVFETFMF PCQHCERKFTTKQGLERHMHIHISTVNHAFKCKYCGKAFGTQINRRRHERRHEAGLKRKP SQTLQPSEDLADGKASGENVASKDDSSPPSLGPDCLIMNSEKASQDTINSSVVEENGEVK ELHPCKYCKKVFGTHTNMRRHQRRVHERHLIPKGVRRKGGLEEPQPPAEQAQATQNVYVP STEPEEEGEADDVYIMDISSNISENLNYYIDGKIQTNNNTSNCDVIEMESASADLYGINC LLTPVTVEITQNIKTTQVPVTEDLPKEPLGSTNSEAKKRRTASPPALPKIKAETDSDPMV PSCSLSLPLSISTTEAVSFHKEKSVYLSSKLKQLLQTQDKLTPAGISATEIAKLGPVCVS APASMLPVTSSRFKRRTSSPPSSPQHSPALRDFGKPSDGKAAWTDAGLTSKKSKLESHSD SPAWSLSGRDERETVSPPCFDEYKMSKEWTASSAFSSVCNQQPLDLSSGVKQKAEGTGKT PVQWESVLDLSVHKKHCSDSEGKEFKESHSVQPTCSAVKKRKPTTCMLQKVLLNEYNGID LPVENPADGTRSPSPCKSLEAQPDPDLGPGSGFPAPTVESTPDVCPSSPALQTPSLSSGQ LPPLLIPTDPSSPPPCPPVLTVATPPPPLLPTVPLPAPSSSASPHPCPSPLSNATAQSPL PILSPTVSPSPSPIPPVEPLMSAASPGPPTLSSSSSSSSSSSSFSSSSSSSSPSPPPLSA ISSVVSSGDNLEASLPMISFKQEELENEGLKPREEPQSAAEQDVVVQETFNKNFVCNVCE SPFLSIKDLTKHLSIHAEEWPFKCEFCVQLFKDKTDLSEHRFLLHGVGNIFVCSVCKKEF AFLCNLQQHQRDLHPDKVCTHHEFESGTLRPQNFTDPSKAHVEHMQSLPEDPLETSKEEE ELNDSSEELYTTIKIMASGIKTKDPDVRLGLNQHYPSFKPPPFQYHHRNPMGIGVTATNF TTHNIPQTFTTAIRCTKCGKGVDNMPELHKHILACASASDKKRYTPKKNPVPLKQTVQPK NGVVVLDNSGKNAFRRMGQPKRLNFSVELSKMSSNKLKLNALKKKNQLVQKAILQKNKSA KQKADLKNACESSSHICPYCNREFTYIGSLNKHAAFSCPKKPLSPPKKKVSHSSKKGGHS SPASSDKNSNSNHRRRTADAEIKMQSMQTPLGKTRARSSGPTQVPLPSSSFRSKQNVKFA ASVKSKKPSSSSLRNSSPIRMAKITHVEGKKPKAVAKNHSAQLSSKTSRSLHVRVQKSKA VLQSKSTLASKKRTDRFNIKSRERSGGPVTRSLQLAAAADLSENKREDGSAKQELKDFRN FL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
