Gene:
Gene Symbol:
ANKHD1-EIF4EBP3
Gene Name:
ANKHD1-EIF4EBP3 readthrough
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069110)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399200
Price:
Login for Pricing
UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCTGACTGATAGCGGAGGCGGCGGCACCTCCTTTGAGGAGGACCTGGACTCTGTGGCT
CCGCGATCCGCCCCAGCTGGGGCCTCGGAGCCGCCTCCGCCGGGAGGGGTCGGTCTGGGG
ATCCGCACCGTGAGGCTCTTTGGGGAGGCCGGGCCAGCGTCGGGAGTCGGCAGCAGCGGC
GGCGGCGGCAGCGGCAGCGGTACGGGCGGAGGGGACGCGGCGCTGGATTTCAAGTTGGCG
GCTGCCGTGCTGAGGACCGGGGGTGGAGGTGGTGCCTCTGGCAGTGACGAGGACGAAGTG
TCCGAGGTTGAATCATTTATTTTGGACCAAGAAGATCTGGATAACCCAGTGCTTAAAACA
ACATCAGAGATATTCTTATCAAGTACTGCAGAAGGAGCAGACTTACGCACTGTGGATCCA
GAGACACAGGCACGACTAGAAGCATTGCTAGAAGCAGCAGGAATTGGCAAATTGTCAACT
GCTGATGGTAAAGCTTTTGCAGATCCTGAGGTACTCCGGAGACTGACATCCTCAGTTAGT
TGTGCACTGGATGAAGCTGCTGCTGCACTGACACGGATGAAAGCAGAAAACAGCCACAAT
GCAGGACAAGTGGACACTCGCAGTCTAGCAGAAGCTTGTTCAGATGGGGATGTTAATGCT
GTTCGTAAATTGCTAGATGAAGGCAGAAGTGTAAATGAACATACAGAAGAAGGAGAAAGC
CTGCTGTGTTTGGCTTGTTCAGCAGGGTATTATGAATTAGCACAAGTATTGCTTGCTATG
CATGCTAATGTTGAAGATCGAGGGAATAAAGGAGACATAACTCCCCTGATGGCAGCTTCC
AGTGGAGGTTACTTAGATATTGTGAAATTATTACTTCTTCATGATGCTGATGTCAACTCC
CAGTCTGCAACAGGAAACACTGCGCTAACTTATGCATGTGCTGGAGGATTTGTTGACATT
GTTAAAGTGCTCCTTAATGAAGGTGCAAATATAGAAGATCATAATGAAAATGGACATACT
CCCTTAATGGAAGCAGCCAGTGCAGGTCATGTGGAAGTTGCAAGAGTTCTTTTAGATCAT
GGTGCAGGCATCAACACTCATTCTAATGAATTCAAAGAAAGTGCTCTAACACTTGCTTGC
TACAAAGGCCATTTGGATATGGTTCGCTTTCTACTTGAAGCTGGTGCAGATCAAGAGCAC
AAAACAGATGAGATGCACACTGCCTTAATGGAGGCCTGCATGGATGGACATGTAGAGGTG
GCACGTTTGCTTTTGGATAGTGGTGCTCAAGTGAACATGCCTGCAGATTCATTTGAATCT
CCATTGACGCTAGCTGCCTGTGGAGGACATGTTGAATTGGCAGCTCTACTTATTGAAAGG
GGAGCAAATCTTGAAGAAGTTAATGATGAAGGATACACTCCCTTGATGGAAGCTGCCCGG
GAAGGACATGAAGAAATGGTGGCACTACTCTTAGCACAAGGAGCAAATATAAATGCCCAG
ACAGAAGAAACTCAAGAAACTGCTCTTACTTTGGCTTGCTGTGGAGGATTTTCTGAAGTT
GCAGACTTTCTTATTAAGGCAGGGGCTGATATAGAACTTGGCTGCTCCACACCTCTGATG
GAGGCATCTCAGGAGGGACACCTGGAATTGGTTAAATATTTGCTGGCTTCTGGCGCTAAT
GTGCATGCTACAACAGCAACAGGAGACACAGCCTTAACCTATGCTTGTGAAAATGGACAT
ACGGATGTTGCAGATGTTTTACTTCAAGCAGGGGCTGATTTAGAACATGAATCTGAAGGT
GGAAGAACACCTTTGATGAAAGCTGCAAGAGCTGGTCATTTGTGCACTGTGCAGTTTCTT
ATTAGCAAAGGTGCCAATGTTAACAGGGCTACAGCCAATAATGATCATACAGTAGTGTCG
CTGGCATGTGCAGGAGGCCACCTGGCAGTTGTTGAGCTTCTCTTGGCTCATGGGGCTGAC
CCTACTCATCGACTCAAGGATGGTTCAACAATGCTCATTGAAGCTGCAAAGGGTGGCCAT
ACTAATGTAGTTTCTTATCTGTTGGATTATCCAAATAATGTTCTGTCAGTTCCCACCACA
GATGTGTCTCAGCTCCCTCCACCTTCTCAAGATCAGTCTCAGGTGCCACGTGTGCCAACG
CATACACTTGCCATGGTTGTACCTCCCCAGGAACCTGACAGAACTTCACAGGAGAACTCT
CCTGCCCTTTTAGGAGTGCAAAAAGGTACATCCAAGCAGAAGTCCAGTTCCCTCCAGGTA
GCAGATCAGGACCTACTGCCATCTTTTCACCCATACCAGCCTTTGGAGTGCATAGTAGAG
GAGACTGAAGGCAAGCTGAATGAACTGGGACAAAGAATTAGTGCTATTGAAAAAGCACAG
CTTAAGTCACTGGAGTTAATTCAAGGTGAACCTCTGAACAAAGATAAGATAGAAGAACTT
AAAAAGAACAGAGAAGAGCAAGTCCAGAAGAAGAAGAAAATATTGAAAGAACTGCAGAAA
GTGGAAAGGCAGTTGCAGATGAAAACACAGCAGCAATTTACCAAAGAATACTTGGAAACC
AAAGGTCAGAAAGACACAGTGTCTCTACACCAACAGTGCTCTCATAGAGGAGTCTTCCCA
GAAGGGGAAGGAGATGGTAGTCTCCCAGAGGATCACTTTTCAGAGTTACCTCAGGTTGAC
ACAATCTTATTTAAAGATAATGATGTTGATGATGAGCAACAGTCTCCACCATCGGCAGAA
CAGATTGATTTTGTCCCAGTCCAGCCTTTATCATCTCCACAGTGTAACTTTTCCAGTGAC
TTAGGTTCTAATGGGACAAATTCTCTTGAACTTCAGAAAGTATCAGGTAATCAGCAGATT
GTAGGACAGCCTCAGATTGCTATTACTGGACATGATCAGGGGCTGTTAGTTCAAGAACCA
GATGGACTAATGGTTGCAACTCCAGCTCAGACGCTTACCGACACTCTTGATGACCTGATA
GCAGCTGTGAGTACCAGAGTGCCCACTGGTTCCAACAGTTCTTCTCAGACCACAGAGTGT
CTTACACCTGAATCCTGTTCGCAGACTACAAGCAATGTGGCTTCCCAATCGATGCCTCCT
GTGTATCCTTCAGTTGACATTGATGCACATACTGAGAGCAATCATGACACAGCATTAACA
CTAGCTTGTGCAGGTGGTCATGAAGAACTTGTATCTGTGCTCATTGCACGGGATGCCAAA
ATTGAACACAGAGACAAAAAAGGTTTCACACCACTAATCCTGGCAGCAACAGCAGGGCAT
GTTGGAGTTGTTGAAATCCTTTTGGATAAAGGTGGAGATATAGAAGCACAGTCTGAACGA
ACTAAGGATACTCCGCTTTCATTGGCATGTTCTGGTGGACGTCAGGAGGTGGTAGACTTG
CTGCTGGCTCGAGGTGCAAATAAAGAACATAGGAACGTATCTGATTATACACCACTGAGT
CTAGCTGCGTCTGGAGGATATGTTAATATCATTAAGATTCTGCTTAATGCTGGGGCAGAA
ATTAATTCAAGGACTGGGAGTAAACTAGGTATTTCTCCCCTGATGTTGGCTGCAATGAAT
GGACATGTTCCTGCAGTAAAATTGCTGCTCGATATGGGTTCAGACATTAATGCCCAAATA
GAGACCAATCGGAACACGGCTCTCACCCTGGCCTGTTTCCAGGGCCGAGCAGAAGTAGTG
AGTTTGCTTCTGGACCGAAAAGCCAATGTTGAACATAGGGCAAAGACGGGTCTTACCCCC
TTGATGGAAGCAGCTTCTGGAGGGTATGCAGAGGTTGGAAGAGTTCTTCTTGATAAAGGA
GCAGATGTTAATGCTCCCCCTGTGCCTTCCTCAAGAGATACTGCTTTAACAATAGCAGCA
GACAAAGGTCACTACAAATTTTGTGAACTCCTGATTCATAGGGGAGCCCACATTGATGTT
CGTAACAAAAAGGGAAATACGCCACTTTGGCTGGCATCCAATGGAGGTCATTTTGATGTT
GTGCAGTTGCTAGTGCAAGCAGGTGCTGATGTGGATGCAGCAGATAACCGGAAAATCACA
CCTCTTATGTCAGCATTTCGCAAGGGTCATGTAAAAGTTGTTCAATATTTGGTAAAGGAA
GTAAATCAGTTCCCTTCTGATATAGAATGCATGAGATACATAGCAACAATTACAGATAAG
GAACTGTTGAAAAAATGTCATCAATGTGTCGAAACCATTGTGAAGGCTAAAGACCAGCAA
GCTGCAGAAGCAAATAAGAATGCGAGTATTCTTTTAAAGGAACTTGATCTGGAAAAGTCA
AGAGAAGAGAGCAGAAAGCAGGCTCTTGCTGCTAAAAGAGAAAAAAGAAAAGAAAAGAGA
AAAAAGAAAAAAGAGGAACAGAAAAGGAAACAGGAAGAAGATGAAGAAAACAAACCTAAG
GAGAATTCGGAACTACCAGAGGATGAAGATGAAGAGGAGAATGATGAAGATGTGGAGCAA
GAAGTTCCCATAGAACCTCCTAGTGCAACCACCACCACTACGATTGGAATCTCTGCAACA
TCTGCAACATTCACAAATGTGTTTGGGAAAAAAAGGGCCAATGTGGTGACAACTCCCAGC
ACCAATCGGAAAAATAAGAAGAACAAAACAAAAGAAACCCCTCCTACAGCACATTTAATT
TTACCAGAACAACATATGTCTTTAGCCCAACAAAAGGCAGATAAAAATAAAATAAATGGA
GAACCTAGAGGTGGTGGTGCAGGTGGGAATAGTGATTCAGATAACTTGGACAGCACAGAC
TGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTTTGTGATGGATGTG
AATTCCTCTAAATACCCCTCACTGCTCCTTCATTCCCAAGAAGAAAAGACAAGTACTGCT
ACTTCCAAAACTCAGACACGACTTGAAGGTGAAGTGACTCCTAATTCCTTGTCAACCAGC
TACAAGACAGTGTCATTGCCATTAAGCTCTCCAAACATAAAGCTGAATCTCACTAGCCCT
AAAAGGGGTCAGAAAAGAGAAGAAGGGTGGAAAGAAGTTGTACGAAGGTCAAAGAAATTG
TCTGTTCCAGCCTCAGTGGTGTCGAGGATAATGGGAAGAGGAGGATGCAACATCACTGCA
ATACAGGATGTTACTGGTGCCCATATTGATGTGGATAAACAAAAAGATAAGAATGGCGAG
AGAATGATCACAATAAGGGGTGGCACAGAATCAACAAGATATGCAGTTCAACTAATCAAT
GCACTCATTCAAGATCCTGCTAAGGAACTGGAAGACTTGATTCCTAAAAATCATATCAGA
ACACCTGCCAGCACCAAATCAATTCATGCTAACTTCTCATCTGGAGTAGGTACCACAGCA
GCTTCCAGTAAAAATGCATTTCCTTTGGGTGCTCCAACTCTTGTAACTTCACAGGCAACA
ACGTTATCTACGTTCCAGCCCGCTAATAAACTTAATAAGAATGTTCCAACAAATGTACGT
TCTTCTTTCCCAGTTTCTCTACCCTTAGCTTATCCTCACCCTCATTTTGCCCTGCTGGCT
GCTCAAACTATGCAACAGATTCGGCATCCTCGCTTACCCATGGCCCAGTTTGGAGGAACC
TTCTCACCTTCTCCTAACACATGGGGACCATTCCCAGTGAGACCTGTGAATCCTGGCAAC
ACAAATAGCTCTCCAAAGCATAATAACACAAGCCGTCTACCTAACCAGAACGGGACTGTT
TTACCCTCAGAGTCTGCTGGACTAGCTACTGCCAGTTGTCCTATCACTGTCTCTTCTGTA
GTTGCTGCCAGTCAGCAACTGTGTGTCACTAATACCCGGACTCCTTCATCAGTCAGAAAG
CAGTTGTTTGCCTGTGTGCCTAAGACAAGTCCTCCAGCAACAGTGATTTCTTCTGTGACA
AGCACTTGTAGTTCCCTGCCTTCTGTCTCCTCTGCACCTATCACTAGCGGGCAAGCTCCC
ACCACATTTCTACCTGCAAGTACTTCTCAAGCACAGCTTTCTTCACAAAAGATGGAGTCT
TTCTCTGCTGTGCCACCCACCAAAGAGAAAGTGTCCACACAGGACCAGCCCATGGCAAAC
CTATGTACCCCATCTTCAACTGCAAACAGTTGCAGTAGCTCTGCCAGCAACACCCCGGGA
GCTCCAGAAACTCACCCATCCAGTAGTCCCACTCCTACTTCCAGTAACACACAAGAGGAG
GCACAGCCATCCAGTGTGTCTGATTTAAGTCCTATGTCAATGCCTTTTGCATCTAACTCA
GAACCTGCTCCATTGACTTTGACATCACCCAGAATGGTTGCTGCTGATAATCAGGACACC
AGTAATTTACCTCAGTTAGCTGTACCAGCACCTCGAGTTTCTCATCGAATGCAGCCCAGA
GGTTCTTTTTACTCCATGGTACCAAATGCAACTATTCACCAGGATCCCCAGTCTATTTTT
GTTACGAATCCAGTTACTTTAACACCACCTCAAGGCCCACCAGCTGCAGTGCAGCTTTCT
TCAGCTGTGAACATTATGAATGGTTCTCAGATGCACATAAACCCAGCAAATAAGTCTTTG
CCACCTACATTTGGCCCAGCCACACTTTTCAATCACTTCAGCAGTCTTTTTGATAGTAGT
CAGGTGCCAGCTAACCAGGGCTGGGGAGATGGTCCACTGTCCTCACGAGTTGCTACAGAT
GCCTCTTTCACTGTTCAGTCAGCGTTCCTGGGTAACTCAGTGCTTGGACACTTGGAAAAC
ATGCACCCTGATAACTCAAAGGCACCTGGCTTCAGACCACCTTCCCAGCGAGTTTCTACT
AGTCCAGTTGGGTTACCATCCATTGACCCATCAGGCAGCTCCCCATCTTCCTCTTCTGCT
CCTCTGGCAAGTTTTTCCGGCATACCAGGAACAAGGGTTTTCCTGCAAGGGCCAGCTCCT
GTTGGGACTCCTAGTTTCAACAGACAACATTTTTCTCCCCATCCTTGGACAAGCGCCTCA
AACTCATCCACTTCTGCCCCACCAACGTTGGGCCAACCAAAAGGAGTCAGTGCCAGTCAA
GATCGAAAGATACCTCCCCCAATTGGAACAGAGAGACTGGCCCGAATTCGGCAAGGAGGG
TCTGTTGCACAAGCCCCGGCGGGGACCAGTTTTGTCGCTCCCGTTGGACACAGTGGAATC
TGGTCATTTGGTGTCAATGCTGTGTCAGAAGGCTTATCAGGTTGGTCGCAATCTGTGATG
GGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATTCTCCCAACATCAGCCA
ATGGAGAGAGATGATTCTGGAATGGTAGCCCCCTCTAACATTTTTCATCAGCCTATGGCA
AGTGGTTTTGTGGATTTTTCTAAAGGTCTGCCAATTTCCATGTATGGAGGCACCATAATA
CCCTCTCATCCTCAGCTTGCTGATGTTCCAGGAGGCCCTCTGTTTAATGGACTTCACAAT
CCAGATCCTGCTTGGAACCCTATGATAAAAGTTATCCAAAATTCAACTGAATGCACTGAT
GCCCAGCAGGCCAGTCTGCTTCCTTCAGTCCCTGCTCTCAAAGGGGAAATCCCATCACCT
CAGCTAACCAGACCGAAGAAGAGAATTGGACGGCCGATGGTGGCCTCTCCTAACCAGAGG
CACCAGGATCATCTACGACCGAAAGTTCCTGCTGGAGTGCAAGAACTCACCCATTGCCCG
GACACCCCCCTGCTGCCTCCCTCAGATTCCCGGGGTCACAACTCCTCCAACAGCCCCTCT
CTCCAAGCTGGAGGAGCTGAAGGAGCAGGAGACAGAGGAAGAGATACCCGATAG
Protein sequence predicted based on nucleotide sequence
MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSG GGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKT TSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLSTADGKAFADPEVLRRLTSSVS CALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGES LLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNS QSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDH GAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEV ARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAR EGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLM EASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLEHESEG GRTPLMKAARAGHLCTVQFLISKGANVNRATANNDHTVVSLACAGGHLAVVELLLAHGAD PTHRLKDGSTMLIEAAKGGHTNVVSYLLDYPNNVLSVPTTDVSQLPPPSQDQSQVPRVPT HTLAMVVPPQEPDRTSQENSPALLGVQKGTSKQKSSSLQVADQDLLPSFHPYQPLECIVE ETEGKLNELGQRISAIEKAQLKSLELIQGEPLNKDKIEELKKNREEQVQKKKKILKELQK VERQLQMKTQQQFTKEYLETKGQKDTVSLHQQCSHRGVFPEGEGDGSLPEDHFSELPQVD TILFKDNDVDDEQQSPPSAEQIDFVPVQPLSSPQCNFSSDLGSNGTNSLELQKVSGNQQI VGQPQIAITGHDQGLLVQEPDGLMVATPAQTLTDTLDDLIAAVSTRVPTGSNSSSQTTEC LTPESCSQTTSNVASQSMPPVYPSVDIDAHTESNHDTALTLACAGGHEELVSVLIARDAK IEHRDKKGFTPLILAATAGHVGVVEILLDKGGDIEAQSERTKDTPLSLACSGGRQEVVDL LLARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMN GHVPAVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVEHRAKTGLTP LMEAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLIHRGAHIDV RNKKGNTPLWLASNGGHFDVVQLLVQAGADVDAADNRKITPLMSAFRKGHVKVVQYLVKE VNQFPSDIECMRYIATITDKELLKKCHQCVETIVKAKDQQAAEANKNASILLKELDLEKS REESRKQALAAKREKRKEKRKKKKEEQKRKQEEDEENKPKENSELPEDEDEEENDEDVEQ EVPIEPPSATTTTTIGISATSATFTNVFGKKRANVVTTPSTNRKNKKNKTKETPPTAHLI LPEQHMSLAQQKADKNKINGEPRGGGAGGNSDSDNLDSTDCNSESSSGGKSQELNFVMDV NSSKYPSLLLHSQEEKTSTATSKTQTRLEGEVTPNSLSTSYKTVSLPLSSPNIKLNLTSP KRGQKREEGWKEVVRRSKKLSVPASVVSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGE RMITIRGGTESTRYAVQLINALIQDPAKELEDLIPKNHIRTPASTKSIHANFSSGVGTTA ASSKNAFPLGAPTLVTSQATTLSTFQPANKLNKNVPTNVRSSFPVSLPLAYPHPHFALLA AQTMQQIRHPRLPMAQFGGTFSPSPNTWGPFPVRPVNPGNTNSSPKHNNTSRLPNQNGTV LPSESAGLATASCPITVSSVVAASQQLCVTNTRTPSSVRKQLFACVPKTSPPATVISSVT STCSSLPSVSSAPITSGQAPTTFLPASTSQAQLSSQKMESFSAVPPTKEKVSTQDQPMAN LCTPSSTANSCSSSASNTPGAPETHPSSSPTPTSSNTQEEAQPSSVSDLSPMSMPFASNS EPAPLTLTSPRMVAADNQDTSNLPQLAVPAPRVSHRMQPRGSFYSMVPNATIHQDPQSIF VTNPVTLTPPQGPPAAVQLSSAVNIMNGSQMHINPANKSLPPTFGPATLFNHFSSLFDSS QVPANQGWGDGPLSSRVATDASFTVQSAFLGNSVLGHLENMHPDNSKAPGFRPPSQRVST SPVGLPSIDPSGSSPSSSSAPLASFSGIPGTRVFLQGPAPVGTPSFNRQHFSPHPWTSAS NSSTSAPPTLGQPKGVSASQDRKIPPPIGTERLARIRQGGSVAQAPAGTSFVAPVGHSGI WSFGVNAVSEGLSGWSQSVMGNHPMHQQLSDPSTFSQHQPMERDDSGMVAPSNIFHQPMA SGFVDFSKGLPISMYGGTIIPSHPQLADVPGGPLFNGLHNPDPAWNPMIKVIQNSTECTD AQQASLLPSVPALKGEIPSPQLTRPKKRIGRPMVASPNQRHQDHLRPKVPAGVQELTHCP DTPLLPPSDSRGHNSSNSPSLQAGGAEGAGDRGRDTR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
