Gene:
Gene Symbol:
COL22A1
Gene Name:
collagen, type XXII, alpha 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069114)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399204
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCGGCCTCCGAGGGAACGCTGTGGCTGGCCTCCTCTGGATGCTGCTGCTGTGGAGT
GGGGGCGGCGGCTGCCAGGCTCAGCGGGCAGGTTGCAAAAGTGTCCACTACGATCTGGTC
TTCCTCCTGGACACCTCCTCCAGCGTGGGCAAGGAGGACTTTGAGAAGGTCCGGCAGTGG
GTGGCCAACCTGGTGGACACCTTCGAGGTGGGCCCCGACCGCACCCGTGTGGGGGTCGTG
CGCTACAGCGACCGGCCCACCACGGCCTTCGAGTTGGGACTCTTTGGCTCGCAGGAGGAG
GTCAAGGCGGCTGCCCGGCGTCTCGCCTACCACGGGGGCAACACCAACACGGGAGACGCG
CTCCGCTACATCACGGCCCGCAGCTTCTCCCCACACGCCGGCGGCCGCCCCAGGGACCGC
GCCTACAAGCAGGTGGCCATCCTGCTCACCGACGGCCGCAGCCAGGACCTGGTGCTGGAC
GCCGCGGCGGCAGCCCACCGCGCTGGCATCCGCATCTTTGCCGTGGGCGTGGGCGAGGCA
CTCAAGGAGGAGCTGGAGGAGATCGCCTCAGAGCCCAAGTCCGCCCACGTCTTCCACGTG
TCCGACTTCAATGCCATCGACAAGATCCGGGGCAAGCTGCGGCGCCGTCTTTGTGAAAAT
GTGCTCTGTCCTAGCGTTCGTGTAGAAGGAGATCGCTTTAAGCACACCAATGGAGGAACC
AAGGAAATCACAGGTTTTGACCTGATGGATTTGTTCAGTGTGAAGGAAATCTTGGGGAAG
AGAGAGAATGGAGCTCAGAGTTCCTATGTACGGATGGGATCCTTCCCTGTGGTGCAAAGT
ACTGAGGATGTGTTCCCCCAAGGTTTACCTGATGAGTACGCCTTTGTCACAACCTTCCGG
TTCAGGAAAACCTCTCGGAAGGAAGACTGGTATATCTGGCAGGTCATCGACCAGTACAGC
ATCCCACAGGTCTCCATCCGGCTGGATGGTGAAAACAAGGCAGTCGAGTACAACGCTGTG
GGTGCCATGAAAGATGCTGTCAGGGTGGTCTTCCGAGGTTCTCGGGTCAATGACCTCTTT
GACCGGGACTGGCACAAGATGGCCCTGAGCATCCAGGCCCAGAACGTCTCCCTGCACATT
GACTGTGCGCTGGTGCAGACACTACCCATCGAGGAACGGGAGAACATTGACATCCAGGGC
AAGACTGTGATTGGCAAGCGCCTCTACGACAGTGTGCCCATTGACTTTGACCTACAGCGG
ATTGTGATCTATTGTGACTCGAGACACGCAGAATTGGAGACTTGTTGTGATATCCCCTCG
GGTCCGTGCCAGGTGACCGTGGTGACAGAGCCTCCACCTCCACCCCCACCCCAGCGGCCT
CCCACCCCAGGCAGTGAACAGATTGGGTTTTTGAAGACCATCAACTGCTCCTGCCCAGCT
GGAGAGAAGGGTGAAATGGGAGTTGCTGGCCCCATGGGGCTCCCTGGTCCAAAGGGAGAC
ATAGGAGCCATTGGGCCGGTTGGCGCTCCTGGACCTAAGGGAGAGAAAGGTGATGTGGGC
ATAGGACCTTTTGGCCAAGGGGAAAAGGGTGAAAAGGGTTCCCTGGGCCTGCCCGGCCCC
CCTGGGAGAGACGGCAGCAAAGGCATGAGAGGGGAGCCAGGAGAGCTGGGAGAGCCGGGG
CTGCCGGGTGAGGTCGGCATGCGGGGGCCCCAAGGACCACCTGGACTCCCCGGACCTCCT
GGACGTGTCGGAGCTCCTGGTCTCCAAGGAGAACGAGGTGAAAAGGGAACTCGAGGAGAA
AAGGGAGAGCGAGGCCTGGATGGATTCCCTGGGAAGCCTGGGGACACAGGACAGCAGGGC
AGGCCCGGCCCTTCTGGTGTGGCAGGACCCCAGGGAGAAAAGGGTGACGTGGGACCTGCG
GGGCCACCTGGTGTACCAGGCTCAGTGGTGCAGCAAGAGGGCTTGAAAGGGGAACAGGGA
GCTCCAGGACCCAGAGGTCACCAAGGCGCCCCCGGTCCTCCAGGAGCTCGGGGTCCAATA
GGCCCAGAAGGCAGGGATGGACCTCCTGGTTTGCAAGGTCTCCGAGGGAAGAAAGGTGAC
ATGGGACCACCTGGAATCCCTGGATTGCTGGGGCTGCAGGGCCCTCCAGGACCCCCTGGT
GTCCCAGGCCCCCCTGGACCGGGAGGTTCTCCGGGTTTGCCTGGAGAGATCGGCTTCCCG
GGAAAGCCTGGACCTCCTGGGCCCACGGGACCCCCTGGAAAGGACGGGCCAAATGGACCA
CCAGGTCCGCCAGGAACCAAGGGAGAACCAGGAGAAAGAGGGGAAGATGGTCTGCCTGGA
AAACCAGGCCTTCGGGGAGAAATTGGGGAGCAGGGCCTGGCAGGCCGACCTGGAGAGAAG
GGAGAAGCAGGCCTCCCAGGGGCTCCAGGCTTCCCAGGTGTGAGAGGAGAGAAAGGAGAC
CAGGGAGAAAAAGGTGAACTGGGACTTCCAGGACTGAAAGGTGACCGAGGTGAAAAGGGT
GAAGCTGGTCCTGCAGGCCCTCCCGGGTTACCTGGAACTACATCCCTGTTCACACCACAT
CCACGGATGCCCGGAGAACAAGGGCCCAAAGGAGAGAAGGGCGATCCAGGCCTGCCTGGG
GAACCGGGACTGCAGGGCCGTCCTGGAGAATTGGGGCCTCAGGGACCCACTGGACCACCG
GGTGCCAAGGGACAGGAAGGTGCACATGGGGCTCCTGGAGCAGCTGGAAACCCCGGTGCT
CCCGGACATGTCGGTGCCCCCGGTCCCAGTGGCCCTCCAGGAAGTGTGGGTGCTCCCGGC
CTCAGAGGCACCCCAGGGAAAGATGGGGAGCGTGGTGAGAAGGGTGCAGCGGGGGAAGAA
GGCAGCCCAGGGCCAGTTGGTCCCAGGGGAGATCCTGGTGCTCCTGGGCTCCCTGGGCCG
CCCGGAAAAGGGAAGGATGGAGAGCCGGGACTCCGTGGATCACCTGGACTCCCTGGACCC
CTAGGAACCAAGGCTGCTTGCGGAAAAGTCAGAGGGTCAGAAAACTGTGCACTGGGAGGG
CAATGTGTTAAGGGGGATCGAGGAGCTCCTGGGATCCCTGGTTCTCCTGGCAGCCGTGGT
GACCCAGGCATTGGGGTTGCTGGCCCTCCTGGCCCTTCCGGACCACCAGGAGACAAAGGA
TCCCCGGGATCACGAGGCTTACCTGGATTCCCTGGCCCCCAGGGCCCAGCCGGCCGGGAC
GGTGCACCAGGAAATCCAGGAGAAAGAGGGCCTCCTGGCAAGCCGGGCCTCTCTTCACTA
CTGTCTCCAGGGGACATAAATCTCTTGGCTAAGGATGTGTGCAATGACTGCCCTCCTGGC
CCCCCAGGCCTCCCTGGTCTACCAGGTTTTAAAGGGGACAAAGGTGTCCCAGGAAAGCCA
GGGAGAGAAGGCACAGAAGGGAAAAAGGGAGAGGCTGGGCCTCCAGGCCTACCAGGGCCC
CCAGGAATAGCTGGACCACAGGGAAGTCAAGGAGAACGTGGTGCAGATGGTGAGGTTGGG
CAGAAAGGTGATCAGGGTCATCCTGGAGTTCCAGGTTTCATGGGGCCCCCAGGGAACCCC
GGGCCACCAGGGGCAGATGGAATTGCAGGAGCTGCTGGACCACCAGGAATCCAAGGGTCA
CCTGGGAAAGAAGGCCCTCCTGGCCCCCAAGGCCCATCTGGATTACCCGGAATCCCAGGA
GAAGAAGGCAAAGAGGGCAGAGATGGAAAGCCGGGTCCCCCTGGAGAGCCGGGCAAAGCA
GGAGAGCCAGGTCTACCAGGACCAGAGGGTGCCCGAGGCCCACCTGGCTTCAAGGGACAC
ACAGGCGATTCTGGTGCACCCGGTCCCCGGGGAGAGTCTGGTGCCATGGGGCTTCCTGGT
CAGGAAGGGTTACCAGGAAAAGATGGTGACACTGGACCCACTGGGCCACAGGGTCCCCAA
GGACCAAGGGGCCCACCGGGCAAGAATGGATCACCGGGATCTCCAGGAGAGCCTGGCCCT
TCAGGAACCCCTGGCCAGAAAGGAAGCAAAGGGGAAAATGGCAGCCCAGGACTTCCTGGC
TTCCTGGGTCCCCGTGGGCCTCCGGGAGAACCAGGAGAGAAAGGAGTCCCAGGCAAGGAG
GGGGTCCCTGGGAAGCCTGGAGAGCCTGGATTCAAAGGAGAAAGGGGAGATCCTGGGATC
AAAGGTGACAAAGGACCTCCTGGTGGAAAAGGCCAGCCTGGGGACCCTGGAATCCCAGGC
CACAAAGGCCACACAGGCCTGATGGGTCCCCAAGGACTACCTGGGGAGAATGGACCAGTT
GGACCCCCAGGGCCTCCAGGCCAGCCGGGATTTCCAGGACTGAGGGGGGAGTCTCCATCC
ATGGAAACCCTGCGTCGGCTTATTCAAGAAGAGCTGGGGAAGCAGCTTGAAACCAGACTC
GCCTACCTCCTGGCCCAGATGCCCCCGGCGTACATGAAGTCATCTCAAGGCAGACCTGGG
CCCCCAGGGCCCCCTGGAAAAGATGGGCTTCCAGGCCGGGCCGGCCCCATGGGGGAGCCA
GGTCGTCCTGGGCAGGGGGGTCTGGAAGGACCCTCTGGACCCATAGGTCCCAAAGGTGAG
CGAGGAGCCAAAGGTGACCCAGGTGCACCTGGAGTTGGCCTCCGAGGCGAGATGGGACCC
CCTGGAATCCCAGGTCAACCCGGGGAACCTGGCTATGCTAAAGATGGACTTCCTGGGATC
CCTGGCCCTCAAGGGGAGACAGGACCAGCTGGACATCCTGGCCTCCCAGGACCTCCCGGT
CCCCCAGGCCAATGTGACCCTTCCCAGTGTGCCTACTTCGCCAGCCTTGCTGCCCGGCCG
GGTAATGTGAAGGGTCCCTAG
Protein sequence predicted based on nucleotide sequence
MAGLRGNAVAGLLWMLLLWSGGGGCQAQRAGCKSVHYDLVFLLDTSSSVGKEDFEKVRQW VANLVDTFEVGPDRTRVGVVRYSDRPTTAFELGLFGSQEEVKAAARRLAYHGGNTNTGDA LRYITARSFSPHAGGRPRDRAYKQVAILLTDGRSQDLVLDAAAAAHRAGIRIFAVGVGEA LKEELEEIASEPKSAHVFHVSDFNAIDKIRGKLRRRLCENVLCPSVRVEGDRFKHTNGGT KEITGFDLMDLFSVKEILGKRENGAQSSYVRMGSFPVVQSTEDVFPQGLPDEYAFVTTFR FRKTSRKEDWYIWQVIDQYSIPQVSIRLDGENKAVEYNAVGAMKDAVRVVFRGSRVNDLF DRDWHKMALSIQAQNVSLHIDCALVQTLPIEERENIDIQGKTVIGKRLYDSVPIDFDLQR IVIYCDSRHAELETCCDIPSGPCQVTVVTEPPPPPPPQRPPTPGSEQIGFLKTINCSCPA GEKGEMGVAGPMGLPGPKGDIGAIGPVGAPGPKGEKGDVGIGPFGQGEKGEKGSLGLPGP PGRDGSKGMRGEPGELGEPGLPGEVGMRGPQGPPGLPGPPGRVGAPGLQGERGEKGTRGE KGERGLDGFPGKPGDTGQQGRPGPSGVAGPQGEKGDVGPAGPPGVPGSVVQQEGLKGEQG APGPRGHQGAPGPPGARGPIGPEGRDGPPGLQGLRGKKGDMGPPGIPGLLGLQGPPGPPG VPGPPGPGGSPGLPGEIGFPGKPGPPGPTGPPGKDGPNGPPGPPGTKGEPGERGEDGLPG KPGLRGEIGEQGLAGRPGEKGEAGLPGAPGFPGVRGEKGDQGEKGELGLPGLKGDRGEKG EAGPAGPPGLPGTTSLFTPHPRMPGEQGPKGEKGDPGLPGEPGLQGRPGELGPQGPTGPP GAKGQEGAHGAPGAAGNPGAPGHVGAPGPSGPPGSVGAPGLRGTPGKDGERGEKGAAGEE GSPGPVGPRGDPGAPGLPGPPGKGKDGEPGLRGSPGLPGPLGTKAACGKVRGSENCALGG QCVKGDRGAPGIPGSPGSRGDPGIGVAGPPGPSGPPGDKGSPGSRGLPGFPGPQGPAGRD GAPGNPGERGPPGKPGLSSLLSPGDINLLAKDVCNDCPPGPPGLPGLPGFKGDKGVPGKP GREGTEGKKGEAGPPGLPGPPGIAGPQGSQGERGADGEVGQKGDQGHPGVPGFMGPPGNP GPPGADGIAGAAGPPGIQGSPGKEGPPGPQGPSGLPGIPGEEGKEGRDGKPGPPGEPGKA GEPGLPGPEGARGPPGFKGHTGDSGAPGPRGESGAMGLPGQEGLPGKDGDTGPTGPQGPQ GPRGPPGKNGSPGSPGEPGPSGTPGQKGSKGENGSPGLPGFLGPRGPPGEPGEKGVPGKE GVPGKPGEPGFKGERGDPGIKGDKGPPGGKGQPGDPGIPGHKGHTGLMGPQGLPGENGPV GPPGPPGQPGFPGLRGESPSMETLRRLIQEELGKQLETRLAYLLAQMPPAYMKSSQGRPG PPGPPGKDGLPGRAGPMGEPGRPGQGGLEGPSGPIGPKGERGAKGDPGAPGVGLRGEMGP PGIPGQPGEPGYAKDGLPGIPGPQGETGPAGHPGLPGPPGPPGQCDPSQCAYFASLAARP GNVKGP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
