Gene:
Gene Symbol:
ZFYVE16
Gene Name:
zinc finger, FYVE domain containing 16
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399306
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GGATCTGGCACTCCCAGGACTCCCGGCCGGGGTAGCTCTTCACTCCTCAGCGCGACGTCG
TGTCGAGTTCCCAAAAAGCTCCGCAGGGGCTGTAGGGAGGCATACAAGAATTAAATTCTG
AATAAGTCTGCAGGTAGGATGGACAGTTATTTTAAAGCAGCTGTCAGTGACTTGGACAAA
CTCCTTGATGATTTTGAACAGAACCCAGATGAACAAGATTATCTCCAAGATGTACAAAAT
GCATATGATTCTAACCACTGCTCAGTTTCTTCAGAGTTGGCTTCCTCACAGCGAACTTCA
TTGCTCCCAAAAGACCAAGAGTGCGTTAATAGTTGTGCCTCATCAGAAACAAGCTATGGA
ACAAATGAGAGTTCCCTGAATGAAAAAACACTCAAGGGACTTACTTCTATACAAAATGAA
AAAAATGTAACAGGACTTGATCTTCTTTCTTCTGTGGATGGTGGTACTTCAGATGAAATC
CAGCCGTTATATATGGGACGATGTAGTAAACCTATCTGTGATCTGATAAGTGACATGGGT
AACTTAGTTCATGCAACCAATAGTGAAGAAGATATTAAAAAATTATTGCCAGATGATTTT
AAGTCTAATGCAGATTCCTTGATTGGATTGGATTTATCTTCAGTGTCAGATACTCCCTGT
GTTTCTTCAACAGACCATGATAGTGATACTGTCAGAGAACAACAGAATGATACCAGTTCT
GAATTACAAAATAGAGAAATCGGAGGAATCAAAGAATTGGGTATAAAAGTAGATACAACA
CTTTCAGATTCCTATAATTACAGTGGAACAGAAAATTTAAAAGATAAAAAGATCTTTAAT
CAGTTAGAATCAATTGTTGATTTTAACATGTCATCTGCTTTGACTCGACAAAGTTCCAAA
ATGTTTCATGCCAAAGACAAGCTACAACACAAGAGCCAGCCATGTGGATTACTAAAAGAT
GTTGGCTTAGTAAAAGAGGAAGTAGATGTGGCAGTCATAACTGCCGCAGAATGTTTAAAA
GAAGAGGGCAAGACAAGTGCTTTGACCTGCAGCCTTCCGAAAAATGAAGATTTATGCTTA
AATGATTCAAATTCAAGAGATGAAAATTTCAAATTACCTGACTTTTCCTTTCAGGAAGAT
AAGACTGTTATAAAACAATCTGCACAAGAAGACTCAAAAAGTTTAGACCTTAAGGATAAT
GATGTAATCCAAGATTCCTCTTCAGCTTTACATGTTTCCAGTAAAGATGTGCCGTCCTCA
TTGTCCTGTCTTCCTGCGTCTGGGTCTATGTGTGGATCATTAATTGAAAGTAAAGCACGG
GGTGATTTTTTACCTCAGCATGAACATAAAGATAATATACAAGATGCAGTGACTATACAT
GAAGAAATACAGAACAGTGTTGTTCTAGGTGGGGAACCATTCAAAGAGAATGATCTTTTG
AAACAGGAAAAATGTAAAAGCATACTCCTTCAGTCATTAATTGAAGGGATGGAAGACAGA
AAGATAGATCCTGACCAGACAGTAATCAGAGCTGAGTCTTTGGATGGTGGTGACACCAGT
TCTACAGTTGTAGAATCTCAAGAGGGGCTTTCTGGCACTCATGTCCCAGAGTCTTCTGAT
TGTTGTGAAGGTTTTATTAATACTTTTTCAAGCAATGATATGGATGGGCAAGACTTAGAT
TACTTTAATATTGATGAAGGCGCAAAAAGTGGCCCACTAATTAGTGATGCTGAACTTGAT
GCCTTTCTGACAGAACAGTATCTTCAGACCACTAACATAAAGTCTTTTGAAGAAAATGTA
AATGACTCTAAATCGCAAATGAATCAGATAGATATGAAAGGCTTAGATGATGGAAACATC
AATAATATATATTTCAATGCAGAAGCAGGAGCTATTGGGGAAAGTCATGGTATTAATATA
ATTTGTGAAACAGTTGATAAACAAAATACAATAGAAAATGGCCTTTCTTTAGGAGAAAAA
AGCACTATTCCAGTTCAACAAGGGTTACCTACCAGTAAGTCTGAGATTACAAATCAATTA
TCAGTCTCTGATATTAACAGTCAATCTGTTGGAGGGGCCAGACCTAAGCAATTGTTTAGC
CTTCCATCAAGAACAAGGAGTTCAAAGGACCTGAATAAGCCAGATGTTCCAGATACAATA
GAAAGTGAACCCAGCACAGCAGATACCGTTGTTCCAATCACTTGTGCTATAGATTCTACA
GCTGATCCACAGGTTAGCTTCAACTCTAATTACATTGATATAGAAAGTAATTCTGAAGGT
GGATCTAGTTTCGTAACTGCAAATGAAGATTCTGTACCTGAAAACACTTGCAAAGAAGGC
TTGGTTTTGGGCCAGAAACAGCCTACTTGGGTTCCTGATTCAGAAGCTCCAAACTGTATG
AACTGCCAAGTCAAATTTACTTTTACCAAACGGCGACACCATTGCCGAGCATGTGGGAAA
GTATTTTGTGGTGTCTGTTGTAATAGGAAGTGTAAACTGCAATATCTAGAAAAGGAAGCA
AGAGTATGTGTAGTCTGCTATGAAACTATTAGTAAAGCTCAGGCATTTGAAAGGATGATG
AGTCCAACTGGTTCTAATCTTAAGTCTAATCATTCTGATGAATGTACTACTGTCCAGCCT
CCTCAGGAGAACCAAACATCCAGTATACCTTCACCAGCAACTTTGCCAGTCTCAGCACTT
AAACAACCAGGTGTTGAAGGACTATGTTCCAAAGAACAGAAGAGAGTATGGTTTGCAGAT
GGTATATTGCCCAATGGTGAAGTTGCAGATACAACAAAATTATCATCTGGAAGTAAAAGA
TGTTCTGAAGACTTTAGTCCTCTCTCACCTGATGTGCCTATGACAGTAAACACAGTGGAT
CATTCCCATTCTACTACAGTGGAAAAGCCAAACAATGAGACAGGAGATATTACAAGAAAT
GAGATAATTCAGAGTCCTATTTCTCAGGTTCCATCAGTGGAAAAATTGTCTATGAACACA
GGAAATGAGGGGTTACCTACTTCTGGTTCATTTACACTAGATGATGATGTTTTTGCAGAA
ACTGAAGAACCATCTAGTCCTACTGGTGTCTTAGTTAACAGCAATTTACCTATTGCTAGT
ATTTCAGATTATAGGTTACTGTGTGATATTAACAAGTATGTCTGCAATAAGATTAGTCTT
CTACCTAATGATGAGGACAGTTTGCCCCCACTTCTGGTTGCATCTGGAGAAAAGGGATCA
GTGCCTGTAGTAGAAGAACATCCATCTCATGAGCAGATCATTTTGCTTCTTGAAGGTGAA
GGCTTTCATCCTGTTACATTTGTCCTAAATGCTAATCTACTCGTGAATGTCAAATTCATA
TTTTATTCCTCAGACAAATATTGGTACTTTTCAACCAATGGATTGCATGGCTTGGGACAG
GCAGAAATTATTATTCTATTGTTATGTTTGCCAAATGAAGATACTATTCCTAAGGACATC
TTCAGACTATTTATCACCATATATAAGGATGCTCTAAAAGGAAAATACATAGAAAACTTG
GACAATATTACCTTTACTGAGAGTTTTCTCAGTAGCAAGGATCACGGAGGATTCCTGTTT
ATTACACCTACTTTTCAGAAACTTGATGATCTCTCATTACCAAGTAATCCTTTTCTTTGT
GGAATTCTTATCCAGAAGCTTGAGATTCCCTGGGCAAAGGTTTTTCCTATGCGTTTAATG
TTGAGATTGGGTGCAGAATATAAAGGTAAGTTTTAGAGTAATAAGTTAAATTGCATATTT
TCAAATATACTGAATGTAAAGACTTTCAGGGAATCATTAAAATCAATTTTAATGGATTAA
TGGAACTACTTTTGTTTTGCTATGCTTTTAAAGTTCTAAGACAACCCTCTTCTACTACCA
GGTTGGTCTAGATAACTGTGGTATGTCTTCAAGTTTCTAGATGTTCATAAGTTCTTTTAT
TACCTAAAACTTTTCTTCTTAAATTTCAGAACAAAACAGAAAACTTTCACAGTGCAGGTT
GAGTATGCCATATCTGAAATGTATTGGATCAGTTTTTTGGATTTTGGACTTTTTCAGATT
TTGGAATATTCACAGAGTACATACCAGTTGAGCATCCCAAATTCAAAATCTGAAATGCTC
CAGTGAGCATTTCTTTTGAGCATCACGTTGGCATTTGAAAAAGTTATAGACCGGGCATGG
TGGCTCACGCCTGTACTCCCGGCACTTTGGGAGGCCAAGGCGGGCGGATCACATGAGGTC
AGGAGTTCGAGACCAGCCTGGCCGGCATGGTGGAGCCCTGTCTCTACTAAAAATACAAAA
ATTGGCTGGGTGAGGTGGCGGGCGCCTGTGATTGCAGCTACTCGGGAGGCTGAGGCAGGA
GAATCGCCTGAACCCGGGAGGCAGATTTGCAGTGAGCTAAGATCATGCCTCTGCATTCCA
GCCAGGGTGACAGAGCGAGACTACGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQ ECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMG RCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDH DSDTVREQQNDTSSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIV DFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTS ALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLDLKDNDVIQDS SSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNS VVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVES QEGLSGTHVPESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQ YLQTTNIKSFEENVNDSKSQMNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICETVD KQNTIENGLSLGEKSTIPVQQGLPTSKSEITNQLSVSDINSQSVGGARPKQLFSLPSRTR SSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVSFNSNYIDIESNSEGGSSFVT ANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCRACGKVFCGVC CNRKCKLQYLEKEARVCVVCYETISKAQAFERMMSPTGSNLKSNHSDECTTVQPPQENQT SSIPSPATLPVSALKQPGVEGLCSKEQKRVWFADGILPNGEVADTTKLSSGSKRCSEDFS PLSPDVPMTVNTVDHSHSTTVEKPNNETGDITRNEIIQSPISQVPSVEKLSMNTGNEGLP TSGSFTLDDDVFAETEEPSSPTGVLVNSNLPIASISDYRLLCDINKYVCNKISLLPNDED SLPPLLVASGEKGSVPVVEEHPSHEQIILLLEGEGFHPVTFVLNANLLVNVKFIFYSSDK YWYFSTNGLHGLGQAEIIILLLCLPNEDTIPKDIFRLFITIYKDALKGKYIENLDNITFT ESFLSSKDHGGFLFITPTFQKLDDLSLPSNPFLCGILIQKLEIPWAKVFPMRLMLRLGAE YKGKF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
