Gene:
Gene Symbol:
MED24
Gene Name:
mediator complex subunit 24
Original Clone ID:
FLH169150.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00039950
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGGTGGTCAACCTGAAGCAAGCCATTTTGCAAGCCTGGAAGGAGCGCTGGAGTGAC
TACCAATGGGCAATCAACATGAAGAAATTCTTTCCTAAAGGAGCCACCTGGGATATTCTC
AACCTGGCAGATGCGTTACTAGAGCAGGCCATGATTGGACCATCCCCCAATCCTCTCATC
TTGTCCTACCTGAAGTATGCCATTAGTTCCCAGATGGTGTCCTACTCTTCTGTCCTCACA
GCCATCAGTAAGTTTGATGACTTTTCTCGGGACCTGTGTGTCCAGGCATTGCTGGACATC
ATGGACATGTTTTGTGACCGTCTGAGCTGTCACGGCAAAGCAGAGGAATGCATCGGACTG
TGCCGAGCCCTTCTTAGCGCCCTCCACTGGCTGCTGCGCTGCACGGCAGCCTCTGCAGAG
CGGCTGCGGGAGGGGCTGGAGGCCGGCACTCCAGCCGCTGGGGAGAAGCAGCTTGCCATG
TGCCTTCAGCGCCTGGAGAAAACCCTCAGCAGCACCAAGAACCGGGCCCTGCTGCACATC
GCCAAACTAGAGGAGGCCTCTTCTTGGACTGCCATCGAGCATTCTCTCTTGAAACTTGGA
GAGATCCTGGCCAATCTCAGCAACCCGCAGCTCCGGAGTCAGGCCGAGCAGTGTGGCACC
CTCATTAGGAGCATCCCCACGATGCTGTCTGTGCATGCGGAGCAGATGCACAAGACCGGC
TTCCCCACTGTCCACGCCGTGATCCTGCTCGAGGGCACCATGAACCTGACAGGCGAGACG
CAGTCCCTGGTGGAGCAGCTGACGATGGTGAAGCGCATGCAGCATATCCCCACCCCACTT
TTTGTCCTGGAGATCTGGAAAGCTTGCTTCGTGGGGCTCATTGAGTCTCCCGAGGGTACG
GAGGAGCTCAAGTGGACAGCTTTCACTTTCCTCAAGATTCCACAGGTTTTGGTGAAGTTG
AAGAAGTACTCTCATGGAGACAAGGACTTCACTGAGGATGTCAACTGTGCTTTTGAGTTC
CTGCTGAAGCTCACCCCCTTGTTGGACAAAGCTGACCAGCGCTGCAACTGTGACTGTACA
AACTTCCTGCTCCAAGAATGTGGCAAGCAGGGGCTTCTGTCTGAGGCCAGCGTCAACAAC
CTTATGGCTAAGCGCAAAGCAGACCGAGAGCACGCACCCCAGCAGAAATCGGGAGAGAAT
GCCAACATCCAGCCCAACATCCAGCTGATCCTCCGGGCGGAGCCCACTGTCACAAACATC
CTCAAGACGATGGATGCAGACCACTCTAAGTCACCGGAGGGACTGCTGGGAGTCCTGGGC
CACATGCTGTCCGGGAAGAGTCTGGACTTGCTGCTGGCTGCCGCCGCCGCCACTGGAAAG
CTGAAATCCTTCGCCCGGAAATTCATCAATTTGAATGAATTCACAACCTATGGCAGCGAA
GAAAGCACCAAACCGGCCTCCGTCCGGGCCCTGCTGTTTGACATCTCCTTCCTCATGCTG
TGCCATGTGGCCCAGACCTATGGTTCAGAGGTGATTCTGTCCGAGTCGCGCACAGGAGCT
GAGGTGCCCTTCTTCGAGACCTGGATGCAGACCTGCATGCCTGAGGAGGGCAAGATCCTG
AACCCTGACCACCCCTGCTTCCGCCCCGACTCCACCAAAGTGGAGTCCCTGGTGGCCCTG
CTCAACAACTCCTCGGAGATGAAGCTAGTGCAGATGAAGTGGCATGAGGCCTGTCTCAGC
ATCTCAGCCGCCATCTTGGAAATCCTCAATGCCTGGGAGAATGGGGTCCTGGCCTTCGAG
TCCATCCAGAAAATCACTGATAACATCAAAGGGAAGGTATGCAGTCTGGCGGTGTGTGCT
GTGGCTTGGCTTGTGGCCCACGTCCGGATGCTGGGGCTGGATGAGCGTGAGAAGTCGCTG
CAGATGATCCGCCAGCTGGCAGGGCCACTGTTTAGTGAGAACACCCTGCAGTTCTACAAT
GAGAGGGTGGTGATCATGAACTCGATCCTGGAGCGCATGTGTGCCGACGTGCTGCAGCAG
ACAGCCACGCAGATCAAGTTTCCCTCCACCGGGGTGGACACAATGCCCTACTGGAACCTG
CTGCCCCCCAAGCGGCCCATCAAAGAGGTGCTGACGGACATTTTTGCCAAGGTGCTGGAG
AAGGGCTGGGTGGACAGCCGCTCCATCCACATCTTTGACACCCTGCTGCACATGGGCGGC
GTCTACTGGTTCTGCAACAACCTGATTAAGGAGCTGCTGAAGGAGACGCGGAAGGAGCAC
ACGCTGCGGGCAGTGGAGCTGCTCTACTCCATCTTCTGCCTGGACATGCAGCAAGTGACC
CTGGTCCTGCTGGGCCACATCCTACCTGGCCTGCTCACTGACTCCTCCAAGTGGCACAGC
CTCATGGACCCCCCGGGCACTGCTCTTGCCAAGCTGGCCGTGTGGTGTGCCCTCAGTTCC
TACTCCTCCCACAAGGGACAGGCGTCCACCCGCCAGAAGAAGAGACACCGCGAAGACATT
GAGGATTATATCAGCCTCTTCCCCCTGGACGATGTGCAGCCTTCGAAGTTGATGCGACTG
CTGAGCTCTAATGAGGACGATGCCAACATCCTTTCGAGCCCCACAGACCGATCCATGAGC
AGCTCCCTCTCAGCCTCTCAGCTCCACACGGTCAACATGCGGGACCCTCTGAACCGAGTC
CTGGCCAACCTGTTCCTGCTCATCTCCTCCATCCTGGGGTCTCGCACCGCTGGCCCCCAC
ACCCAGTTCGTGCAGTGGTTCATGGAGGAGTGTGTGGACTGCCTGGAGCAGGGTGGCCGT
GGCAGCGTCCTGCAGTTCATGCCCTTCACCACCGTGTCGGAACTGGTGAAGGTGTCAGCC
ATGTCTAGCCCCAAGGTGGTTCTGGCCATCACGGACCTCAGCCTGCCCCTGGGCCGCCAG
GTGGCTGCTAAAGCCATTGCTGCACTC
Protein sequence predicted based on nucleotide sequence
MKVVNLKQAILQAWKERWSDYQWAINMKKFFPKGATWDILNLADALLEQAMIGPSPNPLI LSYLKYAISSQMVSYSSVLTAISKFDDFSRDLCVQALLDIMDMFCDRLSCHGKAEECIGL CRALLSALHWLLRCTAASAERLREGLEAGTPAAGEKQLAMCLQRLEKTLSSTKNRALLHI AKLEEASSWTAIEHSLLKLGEILANLSNPQLRSQAEQCGTLIRSIPTMLSVHAEQMHKTG FPTVHAVILLEGTMNLTGETQSLVEQLTMVKRMQHIPTPLFVLEIWKACFVGLIESPEGT EELKWTAFTFLKIPQVLVKLKKYSHGDKDFTEDVNCAFEFLLKLTPLLDKADQRCNCDCT NFLLQECGKQGLLSEASVNNLMAKRKADREHAPQQKSGENANIQPNIQLILRAEPTVTNI LKTMDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTYGSE ESTKPASVRALLFDISFLMLCHVAQTYGSEVILSESRTGAEVPFFETWMQTCMPEEGKIL NPDHPCFRPDSTKVESLVALLNNSSEMKLVQMKWHEACLSISAAILEILNAWENGVLAFE SIQKITDNIKGKVCSLAVCAVAWLVAHVRMLGLDEREKSLQMIRQLAGPLFSENTLQFYN ERVVIMNSILERMCADVLQQTATQIKFPSTGVDTMPYWNLLPPKRPIKEVLTDIFAKVLE KGWVDSRSIHIFDTLLHMGGVYWFCNNLIKELLKETRKEHTLRAVELLYSIFCLDMQQVT LVLLGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASTRQKKRHREDI EDYISLFPLDDVQPSKLMRLLSSNEDDANILSSPTDRSMSSSLSASQLHTVNMRDPLNRV LANLFLLISSILGSRTAGPHTQFVQWFMEECVDCLEQGGRGSVLQFMPFTTVSELVKVSA MSSPKVVLAITDLSLPLGRQVAAKAIAAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
276
End on reference sequence
3245
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
