CTPS2 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  CTPS2
Gene Name:  CTP synthase 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100073935)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399907
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1758nts
Open reading frame: 51 to 1808

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGAAGTACA
TCCTGGTCACGGGTGGGGTCATCTCAGGCATTGGTAAAGGGATCATTGCCAGCAGCATTG
GAACGATTCTAAAATCATGTGGACTCCGAGTTACTGCCATAAAAATCGACCCCTATATTA
ACATCGATGCTGGCACTTTTTCACCTTATGAACACGGTGAAGTCTTCGTCTTAAATGATG
GTGGAGAAGTTGATTTAGACCTTGGAAATTATGAAAGATTTTTGGATATTAATCTTTATA
AAGACAACAATATCACCACGGGGAAGATATATCAGCATGTGATCAATAAAGAGAGGCGTG
GTGATTACCTGGGGAAAACAGTGCAAGTTGTCCCTCACATTACTGATGCTGTCCAGGAGT
GGGTTATGAATCAAGCCAAGGTGCCGGTGGATGGTAATAAGGAAGAGCCCCAAATATGCG
TTATTGAGCTGGGAGGCACCATTGGAGACATCGAAGGAATGCCGTTTGTGGAGGCGTTTA
GACAATTCCAGTTTAAGGCGAAAAGAGAGAATTTCTGTAATATCCACGTTAGCCTTGTCC
CACAGCTCAGTGCTACCGGAGAACAAAAAACCAAACCCACCCAAAACAGCGTCCGCGCAC
TGAGGGGTTTAGGCCTGTCTCCAGATCTGATTGTCTGCCGAAGTTCAACGCCCATTGAGA
TGGCCGTGAAGGAGAAGATTTCTATGTTTTGTCACGTGAACCCTGAACAGGTCATATGTA
TCCATGATGTTTCTTCCACATACCGAGTTCCTGTGCTTTTAGAGGAACAAAGCATTGTGA
AATATTTTAAGGAGAGATTGCACCTGCCCATCGGTGATTCTGCAAGTAATTTGCTTTTTA
AGTGGAGAAATATGGCTGACAGGTATGAAAGGTTACAGAAAATATGCTCCATAGCCCTGG
TTGGCAAATACACCAAGCTCAGAGACTGCTACGCCTCTGTGTTCAAAGCCCTGGAACACT
CAGCCCTGGCCATCAACCACAAGTTGAATCTGATGTACATAGACTCCATTGATCTGGAGA
AGATCACTGAAACCGAGGACCCTGTGAAATTTCATGAAGCTTGGCAGAAGCTATGCAAAG
CTGATGGTATTCTTGTGCCTGGAGGCTTTGGAATCAGAGGAACATTGGGAAAACTCCAGG
CGATTTCTTGGGCAAGGACAAAGAAGATTCCTTTTCTGGGAGTTTGTCTTGGGATGCAAC
TAGCAGTGATAGAGTTTGCAAGAAACTGCCTTAACTTGAAAGATGCTGATTCCACAGAGT
TTAGGCCAAATGCCCCAGTTCCTCTGGTGATTGATATGCCCGAGCACAACCCTGGCAATT
TGGGAGGAACAATGAGACTGGGAATAAGAAGAACTGTTTTCAAAACTGAAAATTCAATAT
TAAGGAAACTTTATGGTGATGTTCCTTTTATAGAAGAAAGACACAGACATCGGTTCGAGG
TAAACCCTAACCTGATCAAACAATTTGAGCAGAATGACTTAAGTTTTGTAGGTCAGGATG
TTGATGGAGACAGGATGGAAATCATTGAACTGGCAAATCATCCTTATTTTGTTGGTGTCC
AGTTCCATCCTGAGTTTTCTTCTAGGCCGATGAAGCCTTCCCCTCCGTATCTGGGGCTGT
TACTTGCAGCAACTGGGAACCTGAATGCCTACTTGCAACAGGGTTGCAAACTGTCTTCCA
GTGATAGATACAGTGATGCCAGTGATGACAGCTTTTCAGAGCCAAGGATAGCTGAGTTGG
AAATAAGCTGCCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA

Protein sequence predicted based on nucleotide sequence

MKYILVTGGVISGIGKGIIASSIGTILKSCGLRVTAIKIDPYINIDAGTFSPYEHGEVFV
LNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDA
VQEWVMNQAKVPVDGNKEEPQICVIELGGTIGDIEGMPFVEAFRQFQFKAKRENFCNIHV
SLVPQLSATGEQKTKPTQNSVRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQ
VICIHDVSSTYRVPVLLEEQSIVKYFKERLHLPIGDSASNLLFKWRNMADRYERLQKICS
IALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEKITETEDPVKFHEAWQK
LCKADGILVPGGFGIRGTLGKLQAISWARTKKIPFLGVCLGMQLAVIEFARNCLNLKDAD
STEFRPNAPVPLVIDMPEHNPGNLGGTMRLGIRRTVFKTENSILRKLYGDVPFIEERHRH
RFEVNPNLIKQFEQNDLSFVGQDVDGDRMEIIELANHPYFVGVQFHPEFSSRPMKPSPPY
LGLLLAATGNLNAYLQQGCKLSSSDRYSDASDDSFSEPRIAELEIS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable