BUB1 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  BUB1
Gene Name:  BUB1 mitotic checkpoint serine/threonine kinase
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100073962)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399933
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3255nts
Open reading frame: 51 to 3305

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGACACCC
CGGAAAATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGCAATGACCCTC
TTGGTGAATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAGAATAAAGAAT
ACTTGATAACTTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAGAAATACCACA
ATGACCCAAGATTCATCAGTTATTGTTTAAAATTTGCTGAGTACAACAGTGACCTCCATC
AATTTTTTGAGTTTCTGTACAACCATGGGATTGGAACCCTGTCATCCCCTCTGTACATTG
CCTGGGCGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCATGCCAGTGCTGTCCTTCAGA
GAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATACAGGTTATTTC
AGACACGCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAACCTCTGCATA
ATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAATAACATGGCCT
GCATTTCTAAGAATCAGGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCTTGTGATAAAG
AGTCAAATATGGAACGAAGAGTGATCACGATTTCTAAATCAGAATATTCTGTGCACTCAT
CTTTGGCATCCAAAGTTGATGTTGAGCAGGTTGTTATGTATTGCAAGGAGAAGCTTATTC
GTGGGGAATCAGAATTTTCCTTTGAAGAATTGAGAGCCCAGAAATACAATCAACGGAGAA
AGCATGAGCAATGGGTAAATGAAGACAGACATTATATGAAAAGGAAAGAAGCAAATGCTT
TTGAAGAACAGCTATTAAAACAGAAAATGGATGAACTTCATAAGAAGTTGCATCAGGTGG
TGGAGACATCCCATGAGGATCTGCCCGCTTCCCAGGAAAGGTCCGAGGTTAATCCAGCAC
GTATGGGGCCAAGTGTAGGCTCCCAGCAGGAACTGAGAGCGCCATGTCTTCCAGTAACCT
ATCAGCAGACACCAGTGAACATGGAAAAGAACCCAAGAGAGGCACCTCCTGTTGTTCCTC
CTTTGGCAAATGCTATTTCTGCAGCTTTGGTGTCCCCAGCCACCAGCCAGAGCATTGCTC
CTCCTGTTCCTTTGAAAGCCCAGACAGTAACAGACTCCATGTTTGCAGTGGCCAGCAAAG
ATGCTGGATGTGTGAATAAGAGTACTCATGAATTCAAGCCACAGAGTGGAGCAGAGATCA
AAGAAGGGTGTGAAACACATAAGGTTGCCAACACAAGTTCTTTTCACACAACTCCAAACA
CATCACTGGGAATGGTTCAGGCAACGCCATCCAAAGTGCAGCCATCACCCACCGTGCACA
CAAAAGAAGCATTAGGTTTCATCATGAATATGTTTCAGGCTCCTACACTTCCTGATATTT
CTGATGACAAAGATGAATGGCAATCTCTAGATCAAAATGAAGATGCATTTGAAGCCCAGT
TTCAAAAAAATGTAAGGTCATCTGGGGCTTGGGGAGTCAATAAGATCATCTCTTCTTTGT
CATCTGCTTTTCATGTGTTTGAAGATGGAAACAAAGAAAATTATGGATTACCACAGCCTA
AAAATAAACCCACAGGAGCCAGGACCTTTGGAGAACGCTCTGTCAGCAGACTTCCTTCAA
AACCAAAGGAGGAAGTGCCTCATGCTGAAGAGTTTTTGGATGACTCAACTGTATGGGGTA
TTCGCTGCAACAAAACCCTGGCACCCAGTCCTAAGAGCCCAGGAGACTTCACATCTGCTG
CACAACTTGCGTCTACACCATTCCACAAGCTTCCAGTGGAGTCAGTGCACATTTTAGAAG
ATAAAGAAAATGTGGTAGCAAAACAGTGTACCCAGGCGACTTTGGATTCTTGTGAGGAAA
ACATGGTGGTGCCTTCAAGGGATGGAAAATTCAGTCCAATTCAAGAGAAAAGCCCAAAAC
AGGCCTTGTCGTCTCACATGTATTCAGCATCCTTACTTCGTCTGAGCCAGCCTGCTGCAG
GTGGGGTACTTACCTGTGAGGCAGAGTTGGGCGTTGAGGCTTGCAGACTCACAGACACTG
ACGCTGCCATTGCAGAAGATCCACCAGATGCTATTGCTGGGCTCCAAGCAGAATGGATGC
AGATGAGTTCACTTGGGACTGTTGATGCTCCAAACTTCATTGTTGGGAACCCATGGGATG
ATAAGCTGATTTTCAAACTTTTATCTGGGCTTTCTAAACCAGTGAGTTCCTATCCAAATA
CTTTTGAATGGCAATGTAAACTTCCAGCCATCAAGCCCAAGACTGAATTTCAATTGGGTT
CTAAGCTGGTCTATGTCCATCACCTTCTTGGAGAAGGAGCCTTTGCCCAGGTGTACGAAG
CTACCCAGGGAGATCTGAATGATGCTAAAAATAAACAGAAATTTGTTTTAAAGGTCCAAA
AGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTTGATGGAAAGACTAAAGCCAT
CTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAATGGCAGTGTAT
TAGTAGGAGAGCTCTACAGCTATGGAACATTATTAAATGCCATTAACCTCTATAAAAATA
CCCCTGAAAAAGTGATGCCTCAAGGTCTTGTCATCTCTTTTGCTATGAGAATGCTTTACA
TGATTGAGCAAGTGCATGACTGTGAAATCATTCATGGAGACATTAAACCAGACAATTTCA
TACTTGGAAACGGATTTTTGGAACAGGATGATGAAGATGATTTATCTGCTGGCTTGGCAC
TGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTTCCAAAAGGAACTATATTCACAG
CAAAGTGTGAAACATCTGGTTTTCAGTGTGTTGAGATGCTCAGCAACAAACCATGGAACT
ACCAGATCGATTACTTTGGGGTTGCTGCAACAGTATATTGCATGCTCTTTGGCACTTACA
TGAAAGTGAAAAATGAAGGAGGAGAGTGTAAGCCTGAAGGTCTTTTTAGAAGGCTTCCTC
ATTTGGATATGTGGAATGAATTTTTTCATGTTATGTTGAATATTCCAGATTGTCATCATC
TTCCATCTTTGGATTTGTTAAGGCAAAAGCTGAAGAAAGTATTTCAACAACACTATACTA
ACAAGATTAGGGCCCTACGTAATAGGCTAATTGTACTGCTCTTAGAATGTAAGCGTTCAC
GAAAATACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA

Protein sequence predicted based on nucleotide sequence

MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKK
KYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASA
VLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGN
NMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKE
KLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKL
HQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPP
VVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSG
AEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTL
PDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGL
PQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDF
TSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEK
SPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQA
EWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEF
QLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMER
LKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMR
MLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGT
IFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFR
RLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLEC
KRSRK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable