SMTN (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SMTN
Gene Name:  smoothelin
Original Clone ID:
FLH169866.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
HsCD00040044
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2745nts
Open reading frame: 1 to 2745

ATGGCGGACGAGGCCTTAGCTGGGCTGGATGAGGGAGCCCTTCGGAAGCTGCTGGAGGTC
ACAGCAGATCTGGCAGAGCGGCGGCGCATCCGCTCAGCCATCCGGGAACTGCAGCGGCAG
GAGCTGGAGCGCGAGGAGGAGGCCCTGGCATCCAAGCGTTTCCGTGCCGAGCGGCAGGAC
AACAAGGAGAACTGGCTGCACTCTCAGCAGCGGGAAGCTGAGCAGCGGGCTGCCCTGGCA
CGGCTGGCAGGGCAGCTGGAGTCCATGAACGATGTGGAGGAATTGACTGCACTGTTGCGA
AGCGCTGGTGAGTATGAGGAGCGCAAGCTGATCCGAGCTGCCATCCGCCGTGTACGGGCT
CAGGAGATTGAGGCTGCCACCTTGGCTGGGAGGTTGTACAGCGGGCGTCCCAACAGTGGC
TCAAGAGAGGACAGCAAGGGGCTAGCGGCACACAGGCTGGAACAGTGTGAGGTGCCAGAG
CGAGAGGAACAGGAACAGCAGGCAGAGGTTTCAAAGCCAACCCCCACCCCTGAAGGCACC
AGCCAGGATGTGACCACAGTGACACTCCTGCTGCGAGCCCCACCTGGGAGCACATCCAGC
TCACCTGCCTCACCCAGCAGTTCACCCACCCCTGCCTCTCCTGAGCCTCCATTGGAGCCT
GCCGAGGCCCAGTGCCTTACAGCTGAGGTTCCAGGCAGCCCAGAGCCACCCCCCAGCCCA
CCCAAGACCACCAGCCCTGAGCCTCAGGAGTCTCCAACGCTCCCCAGCACTGAGGGCCAG
GTGGTCAACAAGCTTCTGTCTGGCCCCAAAGAGACCCCTGCTGCCCAGAGCCCCACCAGA
GGCCCCTCTGACACCAAGAGAGCAGACGTGGCTGGACCCCGACCCTGCCAACGCTCCCTG
TCGGTGCTCAGCCCCCGCCAACCAGCCCAGAACCGAGAGTCCACCCCCCTTGCCAGCGGA
CCTTCCTCATTCCAGCGGGCTGGCTCTGTGCGGGATCGTGTCCACAAGTTCACATCTGAT
TCTCCTATGGCTGCTAGGCTCCAGGATGGCACACCCCAGGCTGCCCTAAGTCCCCTGACC
CCCGCAAGGCTCCTGGGCCCCTCCCTCACCAGCACCACCCCTGCCTCCTCCTCCAGCGGC
TCCTCCTCTCGGGGCCCCAGTGATACCTCCTCCCGGTTCAGCAAGGAGCAACGAGGAGTA
GCCCAGCCCCTGGCCCAGCTTCGAAGCTGCCCCCAGGAGGAGGGCCCCAGGGGGCGGGGC
TTGGCTGCTAGGCCCCTTGAAAACAGAGCAGGGGGGCCTGTGGCACGTTCAGAGGAGCCT
GGTGCCCCGCTGCCCGTGGCCGTCGGCACTGCCGAGCCAGGGGGCAGTATGAAGACCACA
TTCACCATCGAGATCAAGGACGGCCGTGGCCAGGCCTCCACAGGCCGGGTGCTGCTGCCC
ACAGGCAACCAGAGGGCAGAACTGACACTGGGGCTGCGGGCGCCCCCGACCCTACTCAGC
ACCAGTAGTGGGGGCAAGAGCACCATCACCCGTGTCAACAGCCCTGGGACCCTGGCTCGG
CTGGGCAGTGTCACTCATGTCACCAGCTTCAGCCATGCCCCCCCCAGTAGCCGAGGAGGC
TGCAGCATCAAGATGGAACCAGAGCCAGCAGAGCCTCTCGCTGCAGCAGTGGAAGCGGCC
AATGGGGCTGAGCAGACCCGAGTGAACAAAGCACCAGAAGGGCGGAGCCCTCTGAGCGCT
GAGGAGCTGATGACTATTGAGGATGAAGGAGTCTTGGACAAGATGCTGGATCAGAGCACG
GACTTTGAAGAGCGGAAGCTCATCCGGGCTGCACTTCGTGAGCTCCGACAAAGGAAGAGA
GACCAGCGGGACAAGGAGCGGGAACGGCGGCTGCAGGAGGCACGGGGCCGGCCAGGGGAG
GGGCGCGGCAACACAGCCACTGAGACCACCACGAGGCACAGCCAGCGGGCAGCTGATGGC
TCTGCTGTCAGCACTGTTACCAAGACTGAGCGGCTCGTCCACTCCAATGATGGCACACGG
ACGGCCCGCACCACCACAGTGGAGTCGAGTTTCGTGAGGCGCTCGGAGAATGGCAGTGGC
AGCACCATGATGCAAACCAAGACCTTCTCCTCTTCCTCCTCATCCAAGAAGATGGGCAGC
ATCTTCGACCGCGAGGACCAGGCCAGCCCACGGGCCGGCAGCCTGGCGGCGCTCGAGAAA
CGGCAGGCCGAGAAGAAGAAAGAGCTGATGAAGGCGCAGAGTCTGCCCAAGACCTCAGCC
TCCCAGGCGCGCAAGGCCATGATTGAGAAGCTGGAGAAGGAGGGCGCGGCCGGCAGCCCT
GGCGGACCCCGCGCAGCCGTGCAGCGATCCACCAGCTTCGGGGTCCCCAACGCCAACAGC
ATCAAGCAGATGCTGCTGGACTGGTGTCGAGCCAAGACTCGCGGCTACGAGCACGTCGAC
ATCCAGAACTTCTCCTCCAGCTGGAGTGATGGGATGGCCTTCTGTGCCCTGGTGCACAAC
TTCTTCCCTGAGGCCTTCGACTATGGGCAGCTTAGCCCTCAGAACCGACGCCAGAACTTC
GAGGTGGCCTTCTCATCTGCGGAGACCCATGCGGACTGCCCGCAGCTCCTGGATACAGAG
GACATGGTGCGGCTTCGAGAGCCTGACTGGAAGTGCGTGTACACGTACATCCAGGAATTC
TACCGCTGTCTGGTCCAGAAGGGGCTGGTAAAAACCAAAAAGTCC

Protein sequence predicted based on nucleotide sequence

MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEEALASKRFRAERQD
NKENWLHSQQREAEQRAALARLAGQLESMNDVEELTALLRSAGEYEERKLIRAAIRRVRA
QEIEAATLAGRLYSGRPNSGSREDSKGLAAHRLEQCEVPEREEQEQQAEVSKPTPTPEGT
SQDVTTVTLLLRAPPGSTSSSPASPSSSPTPASPEPPLEPAEAQCLTAEVPGSPEPPPSP
PKTTSPEPQESPTLPSTEGQVVNKLLSGPKETPAAQSPTRGPSDTKRADVAGPRPCQRSL
SVLSPRQPAQNRESTPLASGPSSFQRAGSVRDRVHKFTSDSPMAARLQDGTPQAALSPLT
PARLLGPSLTSTTPASSSSGSSSRGPSDTSSRFSKEQRGVAQPLAQLRSCPQEEGPRGRG
LAARPLENRAGGPVARSEEPGAPLPVAVGTAEPGGSMKTTFTIEIKDGRGQASTGRVLLP
TGNQRAELTLGLRAPPTLLSTSSGGKSTITRVNSPGTLARLGSVTHVTSFSHAPPSSRGG
CSIKMEPEPAEPLAAAVEAANGAEQTRVNKAPEGRSPLSAEELMTIEDEGVLDKMLDQST
DFEERKLIRAALRELRQRKRDQRDKERERRLQEARGRPGEGRGNTATETTTRHSQRAADG
SAVSTVTKTERLVHSNDGTRTARTTTVESSFVRRSENGSGSTMMQTKTFSSSSSSKKMGS
IFDREDQASPRAGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGAAGSP
GGPRAAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHN
FFPEAFDYGQLSPQNRRQNFEVAFSSAETHADCPQLLDTEDMVRLREPDWKCVYTYIQEF
YRCLVQKGLVKTKKS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 210
End on reference sequence 2957
Mutations:
None
Discrepancy:
g1534a,V512I; a2564g,N855S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable