Gene:
Gene Symbol:
RNF123
Gene Name:
ring finger protein 123
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
generated from ORFeome entry clone (IMAGE:100002259)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00402853
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCATCCAAGGGGGCCGGCATGTCTTTCTCCCGCAAGAGCTATAGGCTGACCTCAGAT
GCTGAGAAATCCAGGGTCACAGGCATTGTGCAGGAGAAGCTGCTGAATGACTACCTGAAC
CGCATCTTTTCCTCTTCTGAACATGCACCCCCAGCAGCCACCAGCAGGAAACCCCTGAAC
TTCCAGAACCTGCCAGAACATTTGGACCAGTTGCTACAGGTGGACAATGAGGAGGAGGAA
AGCCAGGGACAGGTTGAAGGGCGGCTTGGCCCATCCACTGTGGTCCTGGACCACACAGGC
GGCTTTGAGGGGCTTCTCCTGGTGGATGATGACCTGCTGGGGGTGATTGGACACAGCAAC
TTTGGCACCATCCGCTCTACCACATGCGTGTACAAAGGGAAATGGCTCTACGAGGTCCTC
ATCTCCTCCCAGGGGCTCATGCAGATCGGCTGGTGCACCATCAGCTGCCGCTTCAACCAG
GAGGAGGGGGTTGGAGATACACACAACTCCTATGCCTATGATGGCAACCGCGTGCGCAAG
TGGAATGTGACCACAACGAATTATGGCAAGGCGTGGGCAGCGGGGGACATCGTGAGCTGC
CTGATTGACCTGGATGATGGCACTCTGTCCTTCTGCCTGAACGGTGTATCACTGGGCACT
GCCTTTGAGAACCTGTCCAGGGGCCTGGGTATGGCCTACTTCCCAGCCATCAGCCTCTCT
TTCAAGGAGTCCGTGGCCTTCAACTTTGGCAGCCGTCCTCTGCGCTACCCAGTGGCAGGC
TACCGGCCCCTGCAGGACCCACCGAGTGCTGACCTGGTGCGGGCACAGAGGTTGCTGGGC
TGCTTCCGGGCAGTGCTGAGTGTGGAGCTGGACCCTGTGGAGGGGCGGCTGTTGGACAAG
GAGAGCTCCAAGTGGCGGTTGCGGGGCCAGCCCACCGTCCTCCTCACACTGGCCCACATC
TTCCATCACTTTGCACCGCTTCTGCGCAAGGTGTATCTGGTGGAGGCTGTGCTCATGAGC
TTCTTGCTGGGCATCGTGGAGAAGGGCACACCCACACAGGCACAGTCCGTGGTGCACCAG
GTCCTGGACCTCTTGTGGCTCTTCATGGAGGACTACGAGGTACAAGATTGCCTCAAGCAG
TTGATGATGTCTCTGCTTCGGCTGTACCGATTCTCACCCATTGTCCCAGACCTGGGCCTA
CAGATCCATTACCTGCGGCTCACTATCGCCATCCTGAGGCATGAGAAGTCCCGCAAGTTT
CTGCTTAGCAATGTCCTCTTCGACGTGCTCCGCTCCGTCGTCTTCTTTTACATCAAGAGC
CCCCTGCGTGTGGAGGAGGCCGGCCTGCAGGAGCTCATTCCCACCACCTGGTGGCCCCAC
TGCTCCAGTAGGGAGGGCAAAGAGAGCACGGAGATGAAGGAGGAGACCGCAGAGGAGCGG
CTGCGGCGGCGAGCCTACGAACGGGGCTGTCAGCGGCTCAGGAAGCGCATCGAAGTGGTG
GAAGAACTACAGGTCCAGATCCTGAAGCTGCTGCTGGACAATAAAGATGACAATGGGGGT
GAAGCTTCTAGGTATATCTTCCTGACCAAGTTTCGCAAGTTTCTGCAGGAGAACGCCAGT
GGCCGGGGGAACATGCCCATGCTCTGCCCCCCTGAGTACATGGTCTGCTTCTTACACCGG
CTGATCTCTGCCCTGCGCTACTATTGGGATGAATACAAGGCTTCCAATCCTCATGCTTCC
TTCAGTGAGGAGGCCTACATCCCGCCCCAGGTCTTCTATAATGGCAAGGTGGACTACTTT
GACCTGCAGCGCCTGGGGGGCCTCCTCTCGCGCCTGCGGAAGACCCTCAAAGATGACCTT
GCTTCCAAAGCCAACATTGTGATCGACCCACTGGAGCTCCAGTCAACCGCCATGGATGAC
CTAGATGAGGATGAGGAGCCAGCCCCAGCTATGGCCCAGCGCCCCATGCAGGCCCTGGCT
GTTGGGGGGCCACTGCCCCTGCCCCGGCCCGGCTGGCTCAGTTCTCCAACTTTGGGCCGA
GCCAACCGCTTCCTCAGCACAGCGGCTGTGAGCCTCATGACCCCACGGCGGCCTCTGAGC
ACCTCGGAGAAAGTGAAGGTCCGCACGCTGAGCGTGGAGCAGAGGACCCGTGAGGACATT
GAAGGCAGCCACTGGAATGAGGGCTTGCTGCTGGGGCGGCCCCCCGAGGAGCCTGAGCAG
CCCCTCACCGAGAACTCGCTGCTGGAAGTTCTGGATGGGGCGGTCATGATGTACAACCTC
AGCGTACACCAGCAGCTGGGCAGGATGGTGGGTGTCTCCGATGATGTCAATGAATACGCT
ATGGCTCTGAGGGGCACAGAGGACAAGCTCCGCCGGTGCCCCAAGAGGAGGAAGGACATC
CTTGCAGAGTTGACCAAGAGCCAGAAGGTTTTCTCAGAAAAGCTGGACCACCTGAGCCGC
CGTCTTGCCTGGGTCCATGCCACTGTCTACTCCCAGGAGAAGATGCTGGACATCTACTGG
CTGCTGCGCGTCTGCCTGCGGACCATTGAGCACGGTGATCGCACAGGGTCTCTCTTTGCC
TTCATGCCCGAGTTCTACCTGAGCGTGGCCATCAACGGCTACAGTGCTCTCAAGAATTAC
TTTGGTCCCGTGCACAGCATGGAGGAGCTCCCAGGCTATGAAGAGACCCTGACCCGCCTG
GCTGCCATTCTCGCCAAACACTTTGCCGACGCACGCATTGTGGGCACTGACATCCGAGAC
TCACTGATGCAGGCCCTGGCCAGCTACGTGTGCTACCCACACTCCCTGCGGGCTGTGGAG
CGAATCCCCGAGGAGCAGCGTATCGCCATGGTGAGGAACCTCCTGGCGCCCTATGAGCAG
CGGCCCTGGGCCCAGACCAACTGGATCCTGGTGCGGCTCTGTAGGGGCTGTGGCTTCGGG
TACCGCTATACACGGCTGCCACATCTGCTGAAAACCAAACTTGAGGACGCCAATTTGCCC
AGCCTCCAGAAGCCCTGCCCTTCCACCCTGCTGCAGCAGCACATGGCGGACCTCCTACAG
CAGGGTCCTGATGTGGCACCCAGCTTCCTCAACAGCGTCCTCAATCAGCTCAACTGGGCC
TTCTCTGAATTCATTGGCATGATCCAAGAGATCCAGCAGGCTGCTGAGCGCCTGGAGCGG
AACTTTGTGGACAGCCGGCAGCTCAAGGTATGTGCCACCTGCTTTGACCTCTCGGTCAGC
CTGCTGCGTGTCTTGGAGATGACTATCACACTGGTGCCTGAGATATTCCTTGACTGGACC
CGGCCTACCTCTGAGATGCTGCTGCGGCGTCTTGCACAGCTGCTAAACCAGGTGCTGAAC
CGGGTGACAGCTGAGAGGAACCTGTTTGATCGTGTGGTCACCCTACGGCTGCCTGGCCTA
GAGAGCGTGGACCACTATCCCATTCTGGTGGCAGTGACGGGCATCCTGGTGCAGCTCCTG
GTGCGTGGCCCAGCCTCAGAGAGAGAGCAAGCCACATCAGTGCTCCTGGCAGATCCCTGC
TTCCAGCTACGCTCAATACGCTATCTCCTGGGACAGCCAGAGCCCCCAGCACCTGGCACT
GCTCTGCCAGCCCCTGACCGGAAGCGCTTCTCCCTGCAGAGCTATGCGGATTATATCAGT
GCCGATGAGCTGGCCCAAGTGGAACAGATGCTGGCGCACCTGACCTCTGCATCTGCCCAG
GCAGCAGCTGCCTCCCTGCCCACCAGTGAGGAGGACCTCTGCCCCATCTGCTATGCCCAC
CCCATCTCTGCTGTGTTCCAGCCCTGTGGCCACAAGTCCTGCAAAGCCTGTATCAACCAG
CACCTGATGAACAACAAGGACTGCTTCTTCTGCAAAACCACCATCGTGTCTGTAGAGGAC
TGGGAGAAGGGAGCCAATACGAGTACTACCTCCTCAGCTGCC
Protein sequence predicted based on nucleotide sequence
MASKGAGMSFSRKSYRLTSDAEKSRVTGIVQEKLLNDYLNRIFSSSEHAPPAATSRKPLN FQNLPEHLDQLLQVDNEEEESQGQVEGRLGPSTVVLDHTGGFEGLLLVDDDLLGVIGHSN FGTIRSTTCVYKGKWLYEVLISSQGLMQIGWCTISCRFNQEEGVGDTHNSYAYDGNRVRK WNVTTTNYGKAWAAGDIVSCLIDLDDGTLSFCLNGVSLGTAFENLSRGLGMAYFPAISLS FKESVAFNFGSRPLRYPVAGYRPLQDPPSADLVRAQRLLGCFRAVLSVELDPVEGRLLDK ESSKWRLRGQPTVLLTLAHIFHHFAPLLRKVYLVEAVLMSFLLGIVEKGTPTQAQSVVHQ VLDLLWLFMEDYEVQDCLKQLMMSLLRLYRFSPIVPDLGLQIHYLRLTIAILRHEKSRKF LLSNVLFDVLRSVVFFYIKSPLRVEEAGLQELIPTTWWPHCSSREGKESTEMKEETAEER LRRRAYERGCQRLRKRIEVVEELQVQILKLLLDNKDDNGGEASRYIFLTKFRKFLQENAS GRGNMPMLCPPEYMVCFLHRLISALRYYWDEYKASNPHASFSEEAYIPPQVFYNGKVDYF DLQRLGGLLSRLRKTLKDDLASKANIVIDPLELQSTAMDDLDEDEEPAPAMAQRPMQALA VGGPLPLPRPGWLSSPTLGRANRFLSTAAVSLMTPRRPLSTSEKVKVRTLSVEQRTREDI EGSHWNEGLLLGRPPEEPEQPLTENSLLEVLDGAVMMYNLSVHQQLGRMVGVSDDVNEYA MALRGTEDKLRRCPKRRKDILAELTKSQKVFSEKLDHLSRRLAWVHATVYSQEKMLDIYW LLRVCLRTIEHGDRTGSLFAFMPEFYLSVAINGYSALKNYFGPVHSMEELPGYEETLTRL AAILAKHFADARIVGTDIRDSLMQALASYVCYPHSLRAVERIPEEQRIAMVRNLLAPYEQ RPWAQTNWILVRLCRGCGFGYRYTRLPHLLKTKLEDANLPSLQKPCPSTLLQQHMADLLQ QGPDVAPSFLNSVLNQLNWAFSEFIGMIQEIQQAAERLERNFVDSRQLKVCATCFDLSVS LLRVLEMTITLVPEIFLDWTRPTSEMLLRRLAQLLNQVLNRVTAERNLFDRVVTLRLPGL ESVDHYPILVAVTGILVQLLVRGPASEREQATSVLLADPCFQLRSIRYLLGQPEPPAPGT ALPAPDRKRFSLQSYADYISADELAQVEQMLAHLTSASAQAAAASLPTSEEDLCPICYAH PISAVFQPCGHKSCKACINQHLMNNKDCFFCKTTIVSVEDWEKGANTSTTSSAA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
