Gene:
Gene Symbol:
CSMD1
Gene Name:
CUB and Sushi multiple domains 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
generated from ORFeome entry clone (IMAGE:100061777)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00403033
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACTGCGTGGAGGAGATTCCAGTCGCTGCTCCTGCTTCTCGGGCTGCTGGTGCTGTGC
GCGAGGCTCCTCACTGCAGCGAAGGGTCAGAACTGTGGAGGCTTAGTCCAGGGTCCCAAT
GGCACTATTGAGAGCCCAGGGTTTCCTCACGGGTATCCGAACTATGCCAACTGCACCTGG
ATCATCATCACGGGCGAGCGCAATAGGATACAGTTGTCCTTCCATACCTTTGCTCTTGAA
GAAAATTTTGATATTTTATCAGTTTACGATGGACAGCCTCAACAAGGGAATTTAAAAGTG
AGATTATCGGGATTTCAGCTGCCCTCCTCTATAGTGAGTACAGGATCTATCCTCACTCTG
TGGTTCACGACAGACTTCGCTGTGAGTGCCCAAGGTTTCAAAGCATTATATGAAGTTTTA
CCTAGCCACACTTGTGGAAATCCTGGAGAAATCCTGAAAGGAGTTCTGCATGGAACGAGA
TTCAACATAGGAGACAAAATCCGGTACAGCTGCCTCCCTGGCTACATCTTGGAAGGCCAC
GCCATCCTGACCTGCATCGTCAGCCCAGGAAATGGTGCATCGTGGGACTTCCCAGCTCCC
TTTTGCAGAGCTGAGGGAGCCTGCGGAGGAACCTTACGCGGGACCAGCAGCTCCATCTCC
AGCCCGCACTTCCCTTCAGAGTACGAGAACAACGCGGACTGCACCTGGACCATTCTGGCT
GAGCCCGGGGACACCATTGCGCTGGTCTTCACTGACTTTCAGCTAGAAGAAGGATATGAT
TTCTTAGAGATCAGTGGCACGGAAGCTCCATCCATATGGCTAACTGGCATGAACCTCCCC
TCTCCAGTTATCAGTAGCAAGAATTGGCTACGACTCCATTTCACCTCTGACAGCAACCAC
CGACGCAAAGGATTTAACGCTCAGTTCCAAGTGAAAAAGGCGATTGAGTTGAAGTCAAGA
GGAGTCAAGATGCTGCCCAGCAAGGATGGAAGCCATAAAAACTCTGTCTTGAGCCAAGGA
GGTGTTGCATTGGTCTCTCACATGTGTCTAGATCCTGGGATTCCAGAAAATGGTAGAAGA
GCAGGTTCCGACTTCAGTAGGGTTGGTGCAAATGTACAGTTTTCATGTGAGGACAATTAC
GTGCTCCAGGGATCTAAAAGCATCACCTGTCAGAGAGTTACAGAGACGCTCGCTGCTTGG
AGTGACCACAGGCCCATCTGCCGAGCGAGAACATGTGGATCCAATCTGCGTGGGCCCAGC
GGCGTCATTACCTCCCCTAATTATCCGGTTCAGTATGAAGATAATGCACACTGTGTGTGG
GTCATCACCACCACCGACCCGGACAAGGTCATCAAGCTTGCCTTTGAAGAGTTTGAGCTG
GAGCGAGGCTATGACACCCTGACGGTTGGTGATGCTGGGAAGGTGGGAGACACCAGATCG
GTCTTGTACGTGCTCACGGGATCCAGTGTTCCTGACCTCATTGTGAGCATGAGCAACCAG
ATGTGGCTACATCTGCAGTCGGATGATAGCATTGGCTCACCTGGGTTTAAAGCTGTTTAC
CAAGAAATTGAAAAGGGAGGGTGTGGGGATCCTGGAATCCCCGCCTATGGGAAGCGGACG
GGCAGCAGTTTCCTCCATGGAGATACACTCACCTTTGAATGCCCGGCGGCCTTTGAGCTG
GTGGGGGAGAGAGTTATCACCTGTCAGCAGAACAATCAGTGGTCTGGCAACAAGCCCAGC
TGTGTATTTTCATGTTTCTTCAACTTTACGGCATCATCTGGGATTATTCTGTCACCAAAT
TATCCAGAGGAATATGGGAACAACATGAACTGTGTCTGGTTGATTATCTCGGAGCCAGGA
AGTCGAATTCACCTAATCTTTAATGATTTTGATGTTGAGCCTCAGTTTGACTTTCTCGCG
GTCAAGGATGATGGCATTTCTGACATAACTGTCCTGGGTACTTTTTCTGGCAATGAAGTG
CCTTCCCAGCTGGCCAGCAGTGGGCATATAGTTCGCTTGGAATTTCAGTCTGACCATTCC
ACTACTGGCAGAGGGTTCAACATCACTTACACCACATTTGGTCAGAATGAGTGCCATGAT
CCTGGCATTCCTATAAACGGACGACGTTTTGGTGACAGGTTTCTACTCGGGAGCTCGGTT
TCTTTCCACTGTGATGATGGCTTTGTCAAGACCCAGGGATCCGAGTCCATTACCTGCATA
CTGCAAGACGGGAACGTGGTCTGGAGCTCCACCGTGCCCCGCTGTGAAGCTCCATGTGGT
GGACATCTGACAGCGTCCAGCGGAGTCATTTTGCCTCCTGGATGGCCAGGATATTATAAG
GATTCTTTACATTGTGAATGGATAATTGAAGCAAAACCAGGCCACTCTATCAAAATAACT
TTTGACAGATTTCAGACAGAGGTCAATTATGACACCTTGGAGGTCAGAGATGGGCCAGCC
AGTTCGTCCCCACTGATCGGCGAGTACCACGGCACCCAGGCACCCCAGTTCCTCATCAGC
ACCGGGAACTTCATGTACCTGCTGTTCACCACTGACAACAGCCGCTCCAGCATCGGCTTC
CTCATCCACTATGAGAGTGTGACGCTTGAGTCGGATTCCTGCCTGGACCCGGGCATCCCT
GTGAACGGCCATCGCCACGGTGGAGACTTTGGCATCAGGTCCACAGTGACTTTCAGCTGT
GACCCGGGGTACACACTAAGTGACGACGAGCCCCTCGTCTGTGAGAGGAACCACCAGTGG
AACCACGCCTTGCCCAGCTGCGACGCTCTATGTGGAGGCTACATCCAAGGGAAGAGTGGA
ACAGTCCTTTCTCCTGGGTTTCCAGATTTTTATCCAAACTCTCTAAACTGCACGTGGACC
ATTGAAGTGTCTCATGGGAAAGGAGTTCAAATGATCTTTCACACCTTTCATCTTGAGAGT
TCCCACGACTATTTACTGATCACAGAGGATGGAAGTTTTTCCGAGCCCGTTGCCAGGCTC
ACCGGGTCGGTGTTGCCTCATACGATCAAGGCAGGCCTGTTTGGAAACTTCACTGCCCAG
CTTCGGTTTATATCAGACTTCTCAATTTCGTACGAGGGCTTCAATATCACATTTTCAGAA
TATGACCTGGAGCCATGTGATGATCCTGGAGTCCCTGCCTTCAGCCGAAGAATTGGTTTT
CACTTTGGTGTGGGAGACTCTCTGACGTTTTCCTGCTTCCTGGGATATCGTTTAGAAGGT
GCCACCAAGCTTACCTGCCTGGGTGGGGGCCGCCGTGTGTGGAGTGCACCTCTGCCAAGG
TGTGTGGCCGAATGTGGAGCAAGTGTCAAAGGAAATGAAGGAACATTACTGTCTCCAAAT
TTTCCATCCAATTATGATAATAACCATGAGTGTATCTATAAAATAGAAACAGAAGCCGGC
AAGGGCATCCACCTTAGAACACGAAGCTTCCAGCTGTTTGAAGGAGATACTCTAAAGGTA
TATGATGGAAAAGACAGTTCCTCACGTCCACTGGGCACGTTCACTAAAAATGAACTTCTG
GGGCTGATCCTAAACAGCACATCCAATCACCTGTGGCTAGAGTTCAACACCAATGGATCT
GACACCGACCAAGGTTTTCAACTCACCTATACCAGTTTTGATCTGGTAAAATGTGAGGAT
CCGGGCATCCCTAACTACGGCTATAGGATCCGTGATGAAGGCCACTTTACCGACACTGTA
GTTCTGTACAGTTGCAACCCGGGGTACGCCATGCATGGCAGCAACACCCTGACCTGTTTG
AGTGGAGACAGGAGAGTGTGGGACAAACCACTACCTTCGTGCATAGCGGAATGTGGTGGT
CAGATCCATGCAGCCACATCAGGACGAATATTGTCCCCTGGCTATCCAGCTCCGTATGAC
AACAACCTCCACTGCACCTGGATTATAGAGGCAGACCCAGGAAAGACCATTAGCCTCCAT
TTCATTGTTTTCGACACGGAGATGGCTCACGACATCCTCAAGGTCTGGGACGGGCCGGTG
GACAGTGACATCCTGCTGAAGGAGTGGAGTGGCTCCGCCCTTCCGGAGGACATCCACAGC
ACCTTCAACTCACTCACCCTGCAGTTCGACAGCGACTTCTTCATCAGCAAGTCTGGCTTC
TCCATCCAGTTCTCCACCTCAATTGCAGCCACCTGTAACGATCCAGGTATGCCCCAAAAT
GGCACCCGCTATGGAGACAGCAGAGAGGCTGGAGACACCGTCACATTCCAGTGTGACCCT
GGCTATCAGCTCCAAGGACAAGCCAAAATCACCTGTGTGCAGCTGAATAACCGGTTCTTT
TGGCAACCAGACCCTCCTACATGCATAGCTGCTTGTGGAGGGAATCTGACGGGCCCAGCA
GGTGTTATTTTGTCACCCAACTACCCACAGCCGTATCCTCCTGGGAAGGAATGTGACTGG
AGAGTAAAAGTGAACCCGGACTTTGTCATCGCCTTGATATTCAAAAGTTTCAACATGGAG
CCCAGCTATGACTTCCTACACATCTATGAAGGGGAAGATTCCAACAGCCCCCTCATTGGG
AGTTACCAGGGCTCTCAGGCCCCAGAAAGAATAGAGAGTAGCGGAAACAGCCTGTTTCTG
GCATTTCGGAGTGATGCCTCCGTGGGCCTTTCAGGGTTCGCCATTGAATTTAAAGAGAAA
CCACGGGAAGCTTGTTTTGACCCAGGAAATATAATGAATGGGACAAGAGTTGGAACAGAC
TTCAAGCTTGGCTCCACCATCACCTACCAGTGTGACTCTGGCTATAAGATTCTTGACCCC
TCATCCATCACCTGTGTGATTGGGGCTGATGGGAAACCCTCCTGGGACCAAGTGCTGCCC
TCCTGCAATGCTCCCTGTGGAGGCCAGTACACGGGATCAGAAGGGGTAGTTTTATCACCA
AACTACCCCCATAATTACACAGCTGGTCAAATATGCCTCTATTCCATCACGGTACCAAAG
GAATTCGTGGTCTTTGGACAGTTTGCCTATTTCCAGACAGCCCTGAATGATTTGGCAGAA
TTATTTGATGGAACCCATGCACAGGCCAGACTTCTCAGCTCACTCTCGGGGTCTCACTCA
GGGGAAACATTGCCCTTGGCTACGTCAAATCAAATTCTGCTCCGATTCAGTGCAAAGAGC
GGTGCCTCTGCCCGCGGCTTCCACTTCGTGTATCAAGCTGTTCCTCGTACCAGTGACACC
CAATGCAGCTCTGTCCCCGAGCCCAGATACGGAAGGAGAATTGGTTCTGAGTTTTCTGCC
GGCTCCATCGTCCGATTCGAGTGCAACCCGGGATACCTGCTTCAGGGTTCCACGGCGCTC
CACTGCCAGTCCGTGCCCAACGCCTTGGCACAGTGGAACGACACGATCCCCAGCTGTGTG
GTACCCTGCAGTGGCAATTTCACTCAACGAAGAGGTACAATCCTGTCCCCCGGCTACCCT
GAGCCATACGGAAACAACTTGAACTGTATATGGAAGATCATAGTTACGGAGGGCTCGGGA
ATTCAGATCCAAGTGATCAGTTTTGCCACGGAGCAGAACTGGGACTCCCTTGAGATCCAC
GATGGTGGGGATGTGACCGCACCCAGACTGGGAAGCTTCTCAGGCACCACAGTACCGGCA
CTGCTGAACAGTACTTCCAACCAACTCTACCTGCATTTCCAGTCTGACATTAGTGTGGCA
GCTGCTGGTTTCCACCTGGAATACAAAACTGTAGGTCTTGCTGCATGCCAAGAACCAGCC
CTCCCCAGCAACAGCATCAAAATCGGAGATCGGTACATGGTGAACGACGTGCTCTCCTTC
CAGTGCGAGCCCGGGTACACCCTGCAGGGCCGTTCCCACATTTCCTGTATGCCAGGGACC
GTTCGCCGTTGGAACTATCCGTCTCCCCTGTGCATTGCAACCTGTGGAGGGACGCTGAGC
ACCTTGGGTGGTGTGATCCTGAGCCCCGGCTTCCCAGGTTCTTACCCCAACAACTTAGAC
TGCACCTGGAGGATCTCATTACCCATCGGCTATGGTGCACATATTCAGTTTCTGAATTTT
TCTACCGAAGCTAATCATGACTTCCTTGAAATTCAAAATGGACCTTACCACACCAGCCCC
ATGATTGGACAATTTAGCGGCACGGATCTCCCCGCGGCCCTGCTGAGCACAACGCATGAA
ACCCTCATCCACTTTTATAGTGACCATTCGCAAAACCGGCAAGGATTTAAACTTGCTTAC
CAAGCCTATGAATTACAGAACTGTCCAGATCCACCCCCATTTCAGAATGGGTACATGATC
AACTCGGATTACAGCGTGGGGCAATCAGTATCTTTCGAGTGTTATCCTGGGTACATTCTA
ATAGGCCATCCTGTCCTCACTTGTCAGCATGGGATCAACAGAAACTGGAACTACCCTTTT
CCAAGATGTGATGCCCCTTGTGGGTACAACGTAACTTCTCAGAACGGCACCATCTACTCC
CCTGGCTTTCCTGATGAGTATCCGATCCTGAAGGACTGCATTTGGCTCATCACGGTGCCT
CCAGGGCACGGAGTTTACATCAACTTCACCCTGTTACAGACGGAAGCTGTCAACGATTAC
ATTGCTGTTTGGGACGGTCCCGATCAGAACTCACCCCAGCTGGGAGTTTTCAGTGGCAAC
ACAGCCCTCGAAACGGCGTATAGCTCCACCAACCAAGTCCTGCTCAAGTTCCACAGCGAC
TTTTCAAATGGAGGCTTCTTTGTCCTCAATTTCCACGCATTTCAGCTCAAGAAATGTCAA
CCTCCCCCAGCGGTTCCACAGGCAGAAATGCTTACTGAGGATGATGATTTCGAAATAGGA
GATTTTGTGAAGTACCAGTGCCACCCCGGGTACACCTTGGTGGGGACCGACATTCTGACT
TGCAAGCTCAGTTCCCAGTTGCAGTTTGAGGGTTCTCTCCCAACATGTGAAGCACAATGC
CCAGCAAATGAAGTCCGGACTGGATCATCGGGAGTCATTCTCAGTCCAGGGTATCCGGGT
AATTATTTTAACTCCCAGACTTGCTCTTGGAGTATTAAAGTGGAACCAAACTACAACATT
ACCATCTTTGTGGACACATTTCAAAGTGAAAAGCAGTTTGATGCACTGGAAGTGTTTGAT
GGTTCTTCTGGGCAAAGTCCTCTGCTAGTAGTCTTAAGTGGGAATCATACTGAACAATCA
AATTTTACAAGCAGGAGTAATCAGTTATATCTCCGCTGGTCCACTGACCATGCCACCAGT
AAGAAAGGATTCAAGATTCGCTATGCAGCACCTTACTGCAGTTTGACCCACCCCCTGAAG
AATGGGGGTATTCTAAACAGGACTGCAGGAGCGGTTGGAAGCAAAGTGCATTATTTTTGC
AAGCCTGGATACCGAATGGTCGGCCACAGCAATGCAACCTGTAGACGAAACCCACTTGGC
ATGTACCAGTGGGACTCCCTCACGCCACTCTGCCAGGCTGTGTCCTGTGGAATCCCAGAA
TCCCCAGGAAACGGTTCATTTACCGGGAACGAGTTCACTTTGGACAGTAAAGTGGTCTAT
GAATGTCATGAAGGCTTCAAGCTTGAATCCAGCCAGCAAGCAACAGCCGTGTGTCAAGAA
GATGGGCTGTGGAGTAACAAGGGGAAGCCGCCCATGTGTAAGCCGGTCGCTTGCCCCAGC
ATTGAAGCTCAGCTCTCAGAACATGTCATCTGGAGGCTGGTTTCAGGATCCTTGAATGAG
TACGGTGCTCAAGTATTGCTGAGCTGCAGTCCTGGTTACTACTTAGAAGGCTGGAGGCTC
CTGCGGTGCCAGGCCAATGGGACGTGGAACATAGGAGATGAGAGGCCAAGCTGTCGAGTT
ATCTCGTGTGGAAGCCTTTCCTTTCCCCCAAATGGCAACAAGATTGGAACGTTGACAGTT
TATGGGGCCACAGCTATATTTACGTGCAACACCGGCTACACGCTTGTGGGGTCTCATGTC
AGAGAGTGCTTGGCAAATGGGCTCTGGAGCGGCAGCGAAACTCGATGTCTGGCTGGCCAC
TGCGGTTCCCCAGACCCGATTGTGAACGGTCACATTAGTGGAGATGGCTTCAGTTACAGA
GACACGGTGGTTTACCAGTGCAATCCTGGTTTCCGGCTTGTGGGAACTTCCGTGAGGATA
TGCCTGCAAGACCACAAGTGGTCTGGACAAACGCCTGTCTGTGTCCCCATCACATGTGGT
CACCCTGGAAACCCTGCCCACGGATTCACTAATGGCAGTGAGTTCAACCTGAATGATGTC
GTGAATTTCACCTGCAACACGGGCTATTTGCTGCAGGGCGTGTCTCGAGCCCAGTGTCGG
AGCAACGGCCAGTGGAGTAGCCCTCTGCCCACGTGTCGAGTGGTGAACTGTTCTGATCCA
GGCTTTGTGGAAAATGCCATTCGTCACGGGCAACAGAACTTCCCTGAGAGTTTTGAGTAT
GGAATGAGTATCCTGTACCATTGCAAGAAGGGATTTTACTTGCTGGGATCTTCAGCCTTG
ACCTGTATGGCAAATGGCTTATGGGACCGATCCCTGCCCAAGTGTTTGGCTATATCCTGT
GGACACCCAGGGGTCCCTGCCAACGCCGTCCTCACTGGAGAGCTGTTTACCTATGGCGCC
GTCGTGCACTACTCCTGCAGAGGGAGCGAGAGCCTCATAGGCAACGACACGAGAGTGTGC
CAGGAAGACAGTCACTGGAGCGGGGCACTGCCCCACTGCACAGGAAATAATCCTGGATTC
TGTGGTGATCCGGGGACCCCAGCACATGGGTCTCGGCTTGGTGATGACTTTAAGACAAAG
AGTCTTCTCCGCTTCTCCTGTGAAATGGGGCACCAGCTGAGGGGCTCCCCTGAACGCACG
TGTTTGCTCAATGGGTCATGGTCAGGACTGCAGCCGGTGTGTGAGGCCGTGTCCTGTGGC
AACCCTGGCACACCCACCAACGGAATGATTGTCAGTAGTGATGGCATTCTGTTCTCCAGC
TCGGTCATCTATGCCTGCTGGGAAGGCTACAAGACCTCAGGGCTCATGACACGGCATTGC
ACAGCCAATGGGACCTGGACAGGCACTGCTCCCGACTGCACAATTATAAGTTGTGGGGAT
CCAGGCACACTAGCAAATGGCATCCAGTTTGGGACCGACTTCACCTTCAACAAGACTGTG
AGCTATCAGTGTAACCCAGGCTATGTCATGGAAGCAGTCACATCCGCCACTATTCGCTGT
ACCAAAGACGGCAGGTGGAATCCGAGCAAACCTGTCTGCAAAGCCGTGCTGTGTCCTCAG
CCGCCGCCGGTGCAGAATGGAACAGTGGAGGGAAGTGATTTCCGCTGGGGCTCCAGCATA
AGTTACAGCTGCATGGACGGTTACCAGCTCTCTCACTCCGCCATCCTCTCCTGTGAAGGT
CGCGGGGTGTGGAAAGGAGAGATCCCCCAGTGTCTCCCTGTGTTCTGCGGAGACCCTGGC
ATCCCCGCAGAAGGGCGACTTAGTGGGAAAAGTTTCACCTATAAGTCCGAAGTCTTCTTC
CAGTGCAAATCTCCATTTATACTCGTGGGATCCTCCAGAAGAGTCTGCCAAGCTGACGGC
ACGTGGAGCGGCATACAACCCACCTGCATTGATCCTGCTCATAACACCTGCCCAGACCCT
GGTACGCCACACTTTGGAATACAGAATAGCTCCAGAGGCTATGAGGTTGGAAGCACGGTT
TTTTTCAGGTGCAGAAAAGGCTACCATATTCAAGGTTCCACGACTCGCACCTGCCTTGCC
AATTTAACATGGAGTGGGATACAGACCGAATGTATACCTCATGCCTGCAGACAGCCAGAA
ACCCCGGCACACGCGGATGTGAGAGCCATCGATCTTCCTACTTTCGGCTACACCTTAGTG
TACACCTGCCATCCAGGCTTTTTCCTCGCAGGGGGATCTGAGCACAGAACATGTAAAGCA
GACATGAAATGGACAGGAAAGTCGCCTGTGTGTAAAAGTAAAGGAGTGAGAGAAGTTAAT
GAAACAGTTACTAAAACTCCAGTTCCTTCAGATGTCTTTTTCGTCAATTCACTGTGGAAG
GGGTATTATGAATATTTAGGGAAAAGACAACCCGCCACTCTAACTGTTGACTGGTTCAAT
GCAACAAGCAGTAAGGTGAATGCCACCTTCAGCGAAGCCTCGCCAGTGGAGCTGAAGTTG
ACAGGCATTTACAAGAAGGAGGAGGCCCACTTACTCCTGAAAGCTTTTCAAATTAAAGGC
CAGGCAGATATTTTTGTAAGCAAGTTCGAAAATGACAACTGGGGACTAGATGGTTATGTG
TCATCTGGACTTGAAAGAGGAGGATTTACTTTTCAAGGTGACATTCATGGAAAAGACTTT
GGAAAATTTAAGCTAGAAAGGCAAGATCCTTTAAACCCAGATCAAGACTCTTCCAGTCAT
TACCACGGCACCAGCAGTGGCTCTGTGGCGGCTGCCATTCTGGTTCCTTTCTTTGCTCTA
ATTTTATCAGGGTTTGCATTTTACCTCTACAAACACAGAACGAGACCAAAAGTTCAATAC
AATGGCTATGCTGGGCATGAAAACAGCAATGGACAAGCATCGTTTGAAAACCCCATGTAT
GATACAAACTTAAAACCCACAGAAGCCAAGGCTGTGAGGTTTGACACAACTCTGAACACA
GTCTGTACAGTGGTA
Protein sequence predicted based on nucleotide sequence
MTAWRRFQSLLLLLGLLVLCARLLTAAKGQNCGGLVQGPNGTIESPGFPHGYPNYANCTW IIITGERNRIQLSFHTFALEENFDILSVYDGQPQQGNLKVRLSGFQLPSSIVSTGSILTL WFTTDFAVSAQGFKALYEVLPSHTCGNPGEILKGVLHGTRFNIGDKIRYSCLPGYILEGH AILTCIVSPGNGASWDFPAPFCRAEGACGGTLRGTSSSISSPHFPSEYENNADCTWTILA EPGDTIALVFTDFQLEEGYDFLEISGTEAPSIWLTGMNLPSPVISSKNWLRLHFTSDSNH RRKGFNAQFQVKKAIELKSRGVKMLPSKDGSHKNSVLSQGGVALVSHMCLDPGIPENGRR AGSDFSRVGANVQFSCEDNYVLQGSKSITCQRVTETLAAWSDHRPICRARTCGSNLRGPS GVITSPNYPVQYEDNAHCVWVITTTDPDKVIKLAFEEFELERGYDTLTVGDAGKVGDTRS VLYVLTGSSVPDLIVSMSNQMWLHLQSDDSIGSPGFKAVYQEIEKGGCGDPGIPAYGKRT GSSFLHGDTLTFECPAAFELVGERVITCQQNNQWSGNKPSCVFSCFFNFTASSGIILSPN YPEEYGNNMNCVWLIISEPGSRIHLIFNDFDVEPQFDFLAVKDDGISDITVLGTFSGNEV PSQLASSGHIVRLEFQSDHSTTGRGFNITYTTFGQNECHDPGIPINGRRFGDRFLLGSSV SFHCDDGFVKTQGSESITCILQDGNVVWSSTVPRCEAPCGGHLTASSGVILPPGWPGYYK DSLHCEWIIEAKPGHSIKITFDRFQTEVNYDTLEVRDGPASSSPLIGEYHGTQAPQFLIS TGNFMYLLFTTDNSRSSIGFLIHYESVTLESDSCLDPGIPVNGHRHGGDFGIRSTVTFSC DPGYTLSDDEPLVCERNHQWNHALPSCDALCGGYIQGKSGTVLSPGFPDFYPNSLNCTWT IEVSHGKGVQMIFHTFHLESSHDYLLITEDGSFSEPVARLTGSVLPHTIKAGLFGNFTAQ LRFISDFSISYEGFNITFSEYDLEPCDDPGVPAFSRRIGFHFGVGDSLTFSCFLGYRLEG ATKLTCLGGGRRVWSAPLPRCVAECGASVKGNEGTLLSPNFPSNYDNNHECIYKIETEAG KGIHLRTRSFQLFEGDTLKVYDGKDSSSRPLGTFTKNELLGLILNSTSNHLWLEFNTNGS DTDQGFQLTYTSFDLVKCEDPGIPNYGYRIRDEGHFTDTVVLYSCNPGYAMHGSNTLTCL SGDRRVWDKPLPSCIAECGGQIHAATSGRILSPGYPAPYDNNLHCTWIIEADPGKTISLH FIVFDTEMAHDILKVWDGPVDSDILLKEWSGSALPEDIHSTFNSLTLQFDSDFFISKSGF SIQFSTSIAATCNDPGMPQNGTRYGDSREAGDTVTFQCDPGYQLQGQAKITCVQLNNRFF WQPDPPTCIAACGGNLTGPAGVILSPNYPQPYPPGKECDWRVKVNPDFVIALIFKSFNME PSYDFLHIYEGEDSNSPLIGSYQGSQAPERIESSGNSLFLAFRSDASVGLSGFAIEFKEK PREACFDPGNIMNGTRVGTDFKLGSTITYQCDSGYKILDPSSITCVIGADGKPSWDQVLP SCNAPCGGQYTGSEGVVLSPNYPHNYTAGQICLYSITVPKEFVVFGQFAYFQTALNDLAE LFDGTHAQARLLSSLSGSHSGETLPLATSNQILLRFSAKSGASARGFHFVYQAVPRTSDT QCSSVPEPRYGRRIGSEFSAGSIVRFECNPGYLLQGSTALHCQSVPNALAQWNDTIPSCV VPCSGNFTQRRGTILSPGYPEPYGNNLNCIWKIIVTEGSGIQIQVISFATEQNWDSLEIH DGGDVTAPRLGSFSGTTVPALLNSTSNQLYLHFQSDISVAAAGFHLEYKTVGLAACQEPA LPSNSIKIGDRYMVNDVLSFQCEPGYTLQGRSHISCMPGTVRRWNYPSPLCIATCGGTLS TLGGVILSPGFPGSYPNNLDCTWRISLPIGYGAHIQFLNFSTEANHDFLEIQNGPYHTSP MIGQFSGTDLPAALLSTTHETLIHFYSDHSQNRQGFKLAYQAYELQNCPDPPPFQNGYMI NSDYSVGQSVSFECYPGYILIGHPVLTCQHGINRNWNYPFPRCDAPCGYNVTSQNGTIYS PGFPDEYPILKDCIWLITVPPGHGVYINFTLLQTEAVNDYIAVWDGPDQNSPQLGVFSGN TALETAYSSTNQVLLKFHSDFSNGGFFVLNFHAFQLKKCQPPPAVPQAEMLTEDDDFEIG DFVKYQCHPGYTLVGTDILTCKLSSQLQFEGSLPTCEAQCPANEVRTGSSGVILSPGYPG NYFNSQTCSWSIKVEPNYNITIFVDTFQSEKQFDALEVFDGSSGQSPLLVVLSGNHTEQS NFTSRSNQLYLRWSTDHATSKKGFKIRYAAPYCSLTHPLKNGGILNRTAGAVGSKVHYFC KPGYRMVGHSNATCRRNPLGMYQWDSLTPLCQAVSCGIPESPGNGSFTGNEFTLDSKVVY ECHEGFKLESSQQATAVCQEDGLWSNKGKPPMCKPVACPSIEAQLSEHVIWRLVSGSLNE YGAQVLLSCSPGYYLEGWRLLRCQANGTWNIGDERPSCRVISCGSLSFPPNGNKIGTLTV YGATAIFTCNTGYTLVGSHVRECLANGLWSGSETRCLAGHCGSPDPIVNGHISGDGFSYR DTVVYQCNPGFRLVGTSVRICLQDHKWSGQTPVCVPITCGHPGNPAHGFTNGSEFNLNDV VNFTCNTGYLLQGVSRAQCRSNGQWSSPLPTCRVVNCSDPGFVENAIRHGQQNFPESFEY GMSILYHCKKGFYLLGSSALTCMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGA VVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTK SLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSS SVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTIISCGDPGTLANGIQFGTDFTFNKTV SYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSI SYSCMDGYQLSHSAILSCEGRGVWKGEIPQCLPVFCGDPGIPAEGRLSGKSFTYKSEVFF QCKSPFILVGSSRRVCQADGTWSGIQPTCIDPAHNTCPDPGTPHFGIQNSSRGYEVGSTV FFRCRKGYHIQGSTTRTCLANLTWSGIQTECIPHACRQPETPAHADVRAIDLPTFGYTLV YTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKSKGVREVNETVTKTPVPSDVFFVNSLWK GYYEYLGKRQPATLTVDWFNATSSKVNATFSEASPVELKLTGIYKKEEAHLLLKAFQIKG QADIFVSKFENDNWGLDGYVSSGLERGGFTFQGDIHGKDFGKFKLERQDPLNPDQDSSSH YHGTSSGSVAAAILVPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENSNGQASFENPMY DTNLKPTEAKAVRFDTTLNTVCTVV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
