Gene:
Gene Symbol:
SDK2
Gene Name:
sidekick cell adhesion molecule 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
generated from ORFeome entry clone (IMAGE:100068969)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00403303
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAAGGTGGTGGACATCCCCTGTCAGGCCAAAGGTGTGCCGCCGCCCTCCATCACC
TGGTACAAGGACGCAGCCGTGGTGGAGGTGGAGAAGTTGACCCGCTTCCGGCAGCGCAAC
GACGGGGGCCTGCAGATCAGCGGCCTGGTGCCCGATGATACCGGCATGTTCCAGTGCTTC
GCCCGCAATGCAGCCGGCGAGGTGCAAACTTCCACCTACCTGGCTGTCACCAGCATCGCC
CCTAACATCACCAGAGGCCCCCTGGACAGCACGGTGATCGATGGCATGTCAGTGGTGCTA
GCATGTGAGACCTCGGGGGCGCCCCGACCAGCTATCACTTGGCAGAAAGGGGAGCGCATC
TTGGCCAGTGGCTCTGTGCAGCTGCCTCGCTTCACACCCCTGGAGTCGGGCAGCCTCCTC
ATCAGCCCCACACACATCTCCGATGCGGGGACCTACACCTGCCTGGCCACCAACTCTCGG
GGGGTCGATGAGGCCTCAGCAGACCTAGTCGTTTGGGCTCGGACCCGCATCACCAAGCCC
CCCCAGGATCAGAGTGTCATCAAGGGCACCCAGGCCTCCATGGTGTGCGGAGTGACCCAC
GACCCCCGAGTAACCATCAGGTACATCTGGGAGAAGGACGGGGCCACCCTGGGCACGGAG
AGCCATCCTCGTATCCGCCTGGACAGAAACGGCTCCCTGCACATCTCACAGACGTGGTCG
GGAGACATCGGCACGTACACCTGCCGGGTGATCTCAGCAGGAGGCAACGACTCTCGCAGT
GCCCACCTGCGAGTCAGGCAACTGCCCCACGCGCCCGAGCACCCAGTGGCCACTCTCAGC
ACCGTGGAAAGGCGAGCCATCAACCTGACGTGGACCAAGCCCTTTGATGGCAACAGCCCC
CTGATCCGCTACATTCTGGAGATGTCGGAGAACAATGCCCCCTGGACTGTACTCCTGGCC
AGTGTGGACCCCAAAGCTACCTCAGTGACAGTCAAGGGCCTGGTTCCTGCACGCTCCTAC
CAGTTCCGTCTTTGTGCCGTCAACGACGTGGGGAAAGGACAGTTCAGCAAAGACACCGAG
AGGGTCTCCCTCCCCGAGGAGCCCCCCACGGCCCCTCCACAGAACGTCATCGCCAGCGGT
CGAACCAACCAGTCCATCATGATCCAGTGGCAGCCGCCTCCTGAGAGCCACCAGAATGGA
ATTCTCAAGGGTTACATCATCAGGTACTGCCTGGCCGGGCTGCCCGTGGGGTACCAGTTT
AAGAACATCACGGATGCTGATGTGAACAACCTGCTGCTGGAGGATCTCATCATTTGGACC
AACTACGAGATCGAGGTGGCTGCTTACAACAGCGCTGGGCTGGGGGTCTACAGCAGTAAA
GTCACCGAGTGGACGCTGCAGGGAGTTCCCACAGTCCCTCCGGGCAATGTGCACGCGGAA
GCCACCAATTCCACGACCATCCGCTTCACCTGGAACGCCCCCAGCCCCCAGTTCATCAAC
GGCATCAACCAGGGCTACAAGCTGATCGCCTGGGAGCCGGAACAGGAAGAGGAGGTTACC
ATGGTGACCGCCCGGCCTAACTTTCAAGACAGCATCCACGTGGGCTTCGTGTCTGGCCTG
AAGAAGTTCACCGAGTACTTCACCTCAGTGCTGTGTTTCACCACCCCCGGGGACGGGCCA
CGCAGCACCCCGCAGCTGGTGCGCACCCATGAGGATGTGCCTGGGCCCGTGGGACACCTG
AGCTTCAGTGAGATCCTGGACACATCGCTGAAGGTCAGCTGGCAAGAGCCGGGAGAGAAA
AATGGCATCCTCACAGGGTACCGGATCTCCTGGGAGGAGTACAATCGAACCAACACCCGT
GTGACCCACTACCTGCCCAACGTGACCCTGGAGTACCGTGTCACGGGCCTCACCGCGCTC
ACCACCTACACCATCGAGGTGGCCGCCATGACCTCAAAGGGCCAGGGCCAAGTGTCCGCC
TCCACCATCTCCTCTGGGGTGCCCCCAGAACTCCCAGGGCCCCCCACCAACCTGGGCATT
TCCAACATCGGCCCCCGCTCTGTGACCTTGCAGTTCAGGCCAGGCTACGATGGGAAAACC
TCCATCTCCCGCTGGCTGGTGGAAGCCCAGGTAGGCGTGGTTGGGGAGGGAGAGGAGTGG
TTGCTGATCCACCAGCTCTCCAATGAGCCCGATGCCCGCTCCATGGAGGTGCCCGACCTC
AACCCCTTCACCTGCTACAGCTTCCGCATGCGCCAGGTGAACATCGTGGGCACCAGCCCC
CCCAGTCAGCCTTCTAGAAAGATCCAGACCCTGCAGGCACCCCCTGACATGGCCCCAGCC
AATGTGTCTCTGCGCACAGCCAGTGAGACCAGCCTGTGGCTGCGCTGGATGCCTCTCCCG
GAGATGGAATACAATGGGAACCCTGAGTCCGTGGGCTATAAGATCAAGTACAGCCGGTCA
GACGGGCATGGCAAGACGCTGAGCCACGTGGTGCAGGACCGTGTGGAGCGGGACTACACC
ATCGAGGACCTGGAGGAGTGGACAGAGTACCGCGTCCAGGTCCAGGCCTTCAACGCCATC
GGGAGCGGGCCCTGGAGCCAGACGGTGGTGGGCAGGACCCGGGAGTCAGTTCCCTCTTCC
GGCCCCACCAATGTGTCTGCACTGGCCACCACCTCCAGCAGCATGCTGGTGCGCTGGAGC
GAGGTCCCCGAGGCTGATCGCAACGGGCTCGTGCTGGGCTATAAGGTGATGTATAAGGAG
AAGGACTCGGACACCCAGCCCCGATTCTGGCTGGTGGAAGGCAACTCGTCTCGCAGTGCC
CAGCTCACCGGCTTGGGCAAATACGTGCTCTATGAAGTCCAGGTGCTGGCCTTCACACGC
ATCGGGGACGGCAGCCCCAGCCACCCTCCCATCCTGGAGCGGACGCTGGATGATGTCCCA
GGACCACCCATGGGCATCCTGTTCCCAGAGGTGCGGACCACGTCTGTGCGGCTGATCTGG
CAGCCCCCTGCCGCCCCCAATGGCATCATTCTTGCTTACCAGATCACACACCGGCTCAAC
ACCACCACGGCCAACACCGCCACTGTGGAGGTGCTGGCACCCAGCGCCCGGCAGTACACA
GCCACGGGCCTCAAGCCAGAGTCTGTCTACCTGTTCCGCATCACGGCCCAGACCCGCAAG
GGCTGGGGAGAAGCTGCCGAGGCCTTGGTGGTGACCACCGAGAAGAGAGACCGTCCGCAG
CCCCCCAGCAGGCCGATGGTGCAGCAGGAGGATGTGAGAGCACGCAGCGTGCTGCTGTCC
TGGGAGCCAGGGAGCGACGGGCTCTCCCCTGTGCGCTACTACACCATCCAGACCCGCGAG
CTGCCCAGCGGCAGGTGGGCACTGCACTCGGCCTCCGTGAGCCACAATGCCTCCAGCTTC
ATTGTGGACAGGCTGAAGCCCTTCACGTCCTACAAGTTCCGAGTGAAGGCGACCAATGAC
ATTGGCGACAGCGAGTTCAGCGAGGAGTCGGAGTCACTGACCACCCTGCAGGCTGCCCCC
GATGAAGCACCCACCATCCTCTCCGTGACGCCCCACACCACCACCTCCGTGCTAATCCGA
TGGCAGCCGCCAGCAGAGGACAAGATCAATGGCATCCTCCTGGGCTTCCGGATCCGATAC
CGGGAGCTGCTCTATGAAGGACTGAGGGGCTTCACGCTTCGAGGCATCAACAACCCAGGG
GCCACATGGGCTGAGCTTACCTCCATGTACTCCATGCGGAACCTGAGCCGGCCCAGCCTC
ACGCAGTACGAGCTGGACAACCTGAACAAGCACAGGCGGTACGAGATACGGATGAGCGTG
TACAACGCTGTGGGTGAGGGGCCCTCCAGCCCCCCGCAGGAGGTCTTTGTTGGGGAGGCA
GTGCCCACAGCAGCACCTCGTAACGTGGTCGTCCACGGCGCCACGGCCACACAGCTGGAC
GTGACTTGGGAGCCACCTCCGCTGGACAGCCAGAATGGAGACATCCAGGGGTACAAGATT
TATTTCTGGGAAGCCCAGCGGGGGAACCTCACAGAGCGAGTGAAGACGCTTTTCCTGGCT
GAGAACAGCGTGAAGCTCAAGAACTTGACTGGCTACACGGCCTACATGGTCAGCGTGGCC
GCCTTCAACGCCGCTGGGGATGGGCCTCGGAGCACCCCCACCCAAGGCCAGACCCAGCAA
GCAGCCCCCAGCGCTCCCAGCTCGGTCAAGTTCAGTGAGCTGACCACAACCTCAGTGAAT
GTGTCCTGGGAAGCCCCGCAGTTCCCCAATGGCATCCTGGAGGGCTACAGGCTGGTGTAC
GAGCCCTGCAGCCCCGTGGATGGAGTCAGCAAGATCGTGACCGTGGACGTGAAGGGGAAC
AGCCCCCTGTGGCTGAAGGTGAAGGACCTGGCGGAGGGGGTGACCTACAGGTTCCGCATC
AGAGCCAAGACCTTCACCTACGGGCCGGAGATCGAAGCCAACGTCACCACGGGCCCCGGA
GAAGGTGCCCCAGGACCGCCTGGCGTGCCCATCATCGTGCGGTACAGCTCTGCCATCGCC
ATTCACTGGTCCAGCGGAGACCCGGGCAAAGGGCCCATCACCCGCTACGTCATCGAGGCC
AGACCTTCAGACGAGGGACTATGGGACATCCTCATCAAAGACATCCCCAAGGAGGTGAGC
TCCTACACGTTCAGCATGGACATCCTGAAGCCGGGCGTGAGCTATGACTTCCGGGTCATC
GCGGTCAACGACTATGGTTTCGGCACCCCCAGCAGCCCCTCCCAGTCTGTGCCAGCCCAG
AAAGCCAACCCCTTCTATGAGGAGTGGTGGTTCTTGGTGGTCATTGCCCTGGTCGGCCTC
ATCTTCATCCTGCTTCTGGTCTTCGTGCTCATCATCCGGGGCCAGAGCAAGAAGTACGCC
AAGAAGACAGACTCGGGGAACAGTGCCAAGTCTGGAGCCCTAGGCCACAGCGAGATGATG
AGCTTGGATGAAAGCAGCTTCCCTGCCCTGGAACTCAACAACAGGCGGCTCTCCGTCAAG
AACTCTTTCTGCCGAAAGAACGGCCTGTACACCAGGTCTCCCCCCAGGCCCAGCCCAGGC
AGCCTGCACTACTCGGATGAGGATGTCACCAAATACAACGACCTCATCCCTGCAGAGAGC
AGCAGCCTGACGGAGAAGCCCTCAGAAATCTCCGACTCTCAGGGAAGTGACAGCGAGTAC
GAGGTCGACTCAAACCACCAGAAGGCCCACTCCTTTGTCAACCACTACATCAGTGACCCC
ACATACTACAACTCGTGGCGGCGACAGCAGAAGGGCATCTCGAGGGCACAGGCCTACAGC
TACACGGAGAGCGACTCGGGTGAGCCAGACCACACCACCGTCACCAACAGCACCAGCACC
CAGCAGGGCAGCCTCTTTCGGCCCAAGGCCAGTCGGACTCCAACGCCCCAGAACCCCCCT
AACCCCCCAAGTCAGCAGAGCACCCTCTACCGTCCCCCCAGCAGCCTGGCCCCAGGCTCC
CGGGCTCCCATAGCAGGATTTTCATCATTTGTT
Protein sequence predicted based on nucleotide sequence
MEKVVDIPCQAKGVPPPSITWYKDAAVVEVEKLTRFRQRNDGGLQISGLVPDDTGMFQCF ARNAAGEVQTSTYLAVTSIAPNITRGPLDSTVIDGMSVVLACETSGAPRPAITWQKGERI LASGSVQLPRFTPLESGSLLISPTHISDAGTYTCLATNSRGVDEASADLVVWARTRITKP PQDQSVIKGTQASMVCGVTHDPRVTIRYIWEKDGATLGTESHPRIRLDRNGSLHISQTWS GDIGTYTCRVISAGGNDSRSAHLRVRQLPHAPEHPVATLSTVERRAINLTWTKPFDGNSP LIRYILEMSENNAPWTVLLASVDPKATSVTVKGLVPARSYQFRLCAVNDVGKGQFSKDTE RVSLPEEPPTAPPQNVIASGRTNQSIMIQWQPPPESHQNGILKGYIIRYCLAGLPVGYQF KNITDADVNNLLLEDLIIWTNYEIEVAAYNSAGLGVYSSKVTEWTLQGVPTVPPGNVHAE ATNSTTIRFTWNAPSPQFINGINQGYKLIAWEPEQEEEVTMVTARPNFQDSIHVGFVSGL KKFTEYFTSVLCFTTPGDGPRSTPQLVRTHEDVPGPVGHLSFSEILDTSLKVSWQEPGEK NGILTGYRISWEEYNRTNTRVTHYLPNVTLEYRVTGLTALTTYTIEVAAMTSKGQGQVSA STISSGVPPELPGPPTNLGISNIGPRSVTLQFRPGYDGKTSISRWLVEAQVGVVGEGEEW LLIHQLSNEPDARSMEVPDLNPFTCYSFRMRQVNIVGTSPPSQPSRKIQTLQAPPDMAPA NVSLRTASETSLWLRWMPLPEMEYNGNPESVGYKIKYSRSDGHGKTLSHVVQDRVERDYT IEDLEEWTEYRVQVQAFNAIGSGPWSQTVVGRTRESVPSSGPTNVSALATTSSSMLVRWS EVPEADRNGLVLGYKVMYKEKDSDTQPRFWLVEGNSSRSAQLTGLGKYVLYEVQVLAFTR IGDGSPSHPPILERTLDDVPGPPMGILFPEVRTTSVRLIWQPPAAPNGIILAYQITHRLN TTTANTATVEVLAPSARQYTATGLKPESVYLFRITAQTRKGWGEAAEALVVTTEKRDRPQ PPSRPMVQQEDVRARSVLLSWEPGSDGLSPVRYYTIQTRELPSGRWALHSASVSHNASSF IVDRLKPFTSYKFRVKATNDIGDSEFSEESESLTTLQAAPDEAPTILSVTPHTTTSVLIR WQPPAEDKINGILLGFRIRYRELLYEGLRGFTLRGINNPGATWAELTSMYSMRNLSRPSL TQYELDNLNKHRRYEIRMSVYNAVGEGPSSPPQEVFVGEAVPTAAPRNVVVHGATATQLD VTWEPPPLDSQNGDIQGYKIYFWEAQRGNLTERVKTLFLAENSVKLKNLTGYTAYMVSVA AFNAAGDGPRSTPTQGQTQQAAPSAPSSVKFSELTTTSVNVSWEAPQFPNGILEGYRLVY EPCSPVDGVSKIVTVDVKGNSPLWLKVKDLAEGVTYRFRIRAKTFTYGPEIEANVTTGPG EGAPGPPGVPIIVRYSSAIAIHWSSGDPGKGPITRYVIEARPSDEGLWDILIKDIPKEVS SYTFSMDILKPGVSYDFRVIAVNDYGFGTPSSPSQSVPAQKANPFYEEWWFLVVIALVGL IFILLLVFVLIIRGQSKKYAKKTDSGNSAKSGALGHSEMMSLDESSFPALELNNRRLSVK NSFCRKNGLYTRSPPRPSPGSLHYSDEDVTKYNDLIPAESSSLTEKPSEISDSQGSDSEY EVDSNHQKAHSFVNHYISDPTYYNSWRRQQKGISRAQAYSYTESDSGEPDHTTVTNSTST QQGSLFRPKASRTPTPQNPPNPPSQQSTLYRPPSSLAPGSRAPIAGFSSFV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
