EIF2B5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  EIF2B5
Gene Name:  eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Original Clone ID:
FLH177364.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00040356
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2163nts
Open reading frame: 1 to 2163

ATGGCGGCCCCTGTAGTGGCGCCGCCTGGTGTGGTGGTTAGTCGGGCTAACAAGCGCAGC
GGCGCGGGGCCGGGAGGCAGCGGTGGCGGGGGAGCCAGAGGGGCGGAGGAGGAACCGCCG
CCGCCCCTACAAGCAGTTCTGGTGGCCGATAGCTTCGATCGCCGCTTCTTCCCCATCTCC
AAGGACCAGCCTCGGGTCCTCTTGCCCCTGGCCAATGTGGCATTAATTGACTACACTCTG
GAATTCCTGACTGCCACAGGTGTACAGGAAACATTTGTCTTTTGTTGCTGGAAAGCTGCT
CAAATCAAAGAACATTTACTGAAGTCAAAGTGGTGCCGCCCTACATCTCTCAATGTGGTT
CGAATAATTACATCAGAGCTCTATCGATCACTGGGAGATGTCCTCCGTGATGTTGATGCC
AAGGCTTTGGTGCGCTCTGACTTTCTTCTGGTGTATGGGGATGTCATCTCAAACATCAAT
ATCACCAGAGCCCTTGAGGAACACAGGTTGAGACGGAAGCTAGAAAAAAATGTTTCTGTG
ATGACGATGATCTTCAAGGAGTCATCCCCCAGCCACCCAACTCGTTGCCACGAAGACAAT
GTGGTAGTGGCTGTGGATAGTACCACAAACAGGGTTCTCCATTTTCAGAAGACCCAGGGT
CTCCGGCGTTTTGCATTTCCTCTGAGCCTGTTTCAGGGCAGTAGTGATGGAGTGGAGGTT
CGATATGATTTACTGGATTGTCATATCAGCATCTGTTCTCCTCAGGTGGCACAACTCTTT
ACAGACAACTTTGACTACCAAACTCGAGATGACTTTGTGCGAGGTCTCTTAGTGAATGAG
GAGATCCTAGGGAACCAGATCCACATGCACGTAACAGCTAAGGAATATGGTGCCCGTGTC
TCCAACCTACACATGTACTCAGCTGTCTGTGCTGACGTCATCCGCCGATGGGTCTACCCT
CTCACCCCAGAGGCGAACTTCACTGACAGCACCACCCAGAGCTGCACTCATTCCCGGCAC
AACATCTACCGAGGGCCTGAGGTCAGCCTGGGCCATGGCAGCATCCTAGAGGAAAATGTG
CTCCTGGGCTCTGGCACTGTCATTGGCAGCAATTGCTTTATCACCAACAGTGTCATTGGC
CCCGGCTGCCACATTGGTGATAACGTGGTGCTGGACCAGACCTACCTGTGGCAGGGTGTT
CGAGTGGCGGCTGGAGCACAGATCCATCAGTCTCTGCTTTGTGACAATGCTGAGGTCAAG
GAACGAGTGACACTGAAACCACGCTCTGTCCTCACTTCCCAGGTGGTCGTGGGCCCAAAT
ATCACGCTGCCTGAGGGCTCGGTGATCTCTTTGCACCCTCCAGATGCAGAGGAAGATGAA
GATGATGGCGAGTTCAGTGATGATTCTGGGGCTGACCAAGAAAAGGACAAAGTGAAGATG
AAAGGTTACAATCCAGCAGAAGTAGGAGCTGCTGGCAAGGGCTACCTCTGGAAAGCTGCA
GGCATGAACATGGAGGAAGAGGAGGAACTGCAGCAGAATCTGTGGGGACTCAAGATCAAC
ATGGAAGAAGAGAGTGAAAGTGAAAGTGAGCAAAGTATGGATTCTGAGGAGCCGGACAGC
CGGGGAGGCTCCCCTCAGATGGATGACATCAAAGTGTTCCAGAATGAAGTTTTAGGAACA
CTACAGCGGGGCAAAGAGGAGAACATTTCTTGTGACAATCTCGTCCTGGAAATCAACTCT
CTCAAGTATGCCTATAACGTAAGTCTAAAGGAGGTGATGCAGGTACTGAGCCACGTGGTC
CTGGAGTTCCCCCTGCAACAGATGGATTCCCCGCTTGACTCAAGCCGCTACTGTGCCCTG
CTGCTTCCTCTGCTAAAGGCCTGGAGCCCTGTTTTTAGGAACTACATAAAGCGCGCAGCC
GACCATTTGGAAGCGTTAGCAGCCATTGAGGACTTCTTCCTAGAGCATGAAGCTCTTGGT
ATTTCCATGGCCAAGGTACTGATGGCTTTCTACCAGCTGGAGATCCTGGCTGAGGAAACA
ATTCTGAGCTGGTTCAGCCAAAGAGATACAACTGACAAGGGCCAGCAGTTGCGCAAGAAT
CAACAGCTGCAGAGGTTCATCCAGTGGCTAAAAGAGGCAGAAGAGGAGTCATCTGAAGAT
GAC

Protein sequence predicted based on nucleotide sequence

MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPIS
KDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVV
RIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSV
MTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEV
RYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARV
SNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENV
LLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVK
ERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKM
KGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDS
RGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNVSLKEVMQVLSHVV
LEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALG
ISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSED
D

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2216
Start on reference sequence 51
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable