DDX19A (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  DDX19B
Gene Name:  DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
generated from ORFeome entry clone (IMAGE:100073441)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00404310
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1434nts
Open reading frame: 51 to 1484

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGCCACCG
ACTCGTGGGCCCTGGCGGTGGACGAGCAGGAAGCGGCTGTCAAGTCGATGACCAATTTGC
AGATCAAGGAAGAGAAAGTCAAAGCAGATACCAATGGTATTATCAAAACCAGTACCACTG
CCGAGAAAACAGATGAAGAGGAGAAAGAGGACAGAGCTGCCCAGTCCTTACTCAACAAGC
TGATCAGAAGCAACCTTGTTGATAACACAAACCAAGTGGAAGTCCTGCAACGGGATCCAA
ACTCCCCTCTGTACTCGGTGAAGTCGTTTGAAGAGCTTCGGCTGAAACCACAGCTTCTCC
AGGGAGTCTATGCCATGGGCTTCAATCGACCCTCCAAGATACAAGAGAACGCATTACCCA
TGATGCTTGCTGAACCCCCACAGAATCTGATTGCCCAGTCTCAGTCTGGCACTGGTAAAA
CAGCTGCCTTTGTCTTAGCCATGCTCAGCCGAGTGGAGCCATCAGACAGATACCCCCAGT
GTCTGTGCCTCTCCCCAACATATGAGCTGGCGCTTCAAACAGGAAAAGTGATTGAGCAGA
TGGGCAAATTTTACCCAGAACTGAAGCTTGCCTATGCCGTTCGAGGCAATAAATTGGAAA
GAGGCCAGAAGATCAGTGAGCAGATTGTCATTGGCACCCCTGGGACCGTGCTGGACTGGT
GCTCCAAGCTCAAGTTCATTGATCCCAAGAAAATCAAGGTGTTTGTTCTGGATGAGGCTG
ATGTCATGATAGCCACTCAGGGCCACCAAGATCAGAGCATCCGCATCCAGAGGATGCTGC
CCAGGAACTGCCAGATGCTGCTTTTCTCCGCCACCTTTGAAGACTCTGTGTGGAAGTTTG
CCCAGAAAGTGGTCCCAGACCCAAATGTTATCAAACTGAAGCGTGAGGAAGAGACCCTGG
ATACCATCAAGCAGTACTATGTCCTGTGCAGCAGCAGAGACGAGAAGTTCCAGGCCTTGT
GTAACCTCTACGGGGCCATCACCATTGCTCAAGCCATGATCTTCTGCCATACTCGCAAAA
CAGCTAGTTGGCTGGCAGCAGAGCTCTCAAAAGAAGGCCACCAGGTGGCTCTGCTGAGTG
GGGAGATGATGGTGGAGCAGAGGGCTGCGGTGATTGAGCGCTTCCGAGAGGGCAAAGAGA
AGGTTTTGGTGACCACCAACGTGTGTGCCCGCGGCATTGATGTTGAACAAGTGTCTGTCG
TCATCAACTTTGATCTTCCCGTGGACAAGGACGGGAATCCTGACAATGAGACCTACCTGC
ACCGGATCGGGCGCACGGGCCGCTTTGGCAAGAGGGGCCTGGCAGTGAACATGGTGGACA
GCAAGCACAGCATGAACATCCTGAACAGAATCCAGGAGCATTTTAATAAGAAGATAGAAA
GATTGGACACAGATGATTTGGACGAGATTGAGAAAATAGCCAACTGCCCAACTTTCTTGT
ACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA

Protein sequence predicted based on nucleotide sequence

MATDSWALAVDEQEAAVKSMTNLQIKEEKVKADTNGIIKTSTTAEKTDEEEKEDRAAQSL
LNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQEN
ALPMMLAEPPQNLIAQSQSGTGKTAAFVLAMLSRVEPSDRYPQCLCLSPTYELALQTGKV
IEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVL
DEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREE
ETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVA
LLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNE
TYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable