Gene:
Gene Symbol:
ZFYVE16
Gene Name:
zinc finger, FYVE domain containing 16
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
generated from ORFeome entry clone
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00404387
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GGATCTGGCACTCCCAGGACTCCCGGCCGGGGTAGCTCTTCACTCCTCAGCGCGACGTCG
TGTCGAGTTCCCAAAAAGCTCCGCAGGGGCTGTAGGGAGGCATACAAGAATTAAATTCTG
AATAAGTCTGCAGGTAGGATGGACAGTTATTTTAAAGCAGCTGTCAGTGACTTGGACAAA
CTCCTTGATGATTTTGAACAGAACCCAGATGAACAAGATTATCTCCAAGATGTACAAAAT
GCATATGATTCTAACCACTGCTCAGTTTCTTCAGAGTTGGCTTCCTCACAGCGAACTTCA
TTGCTCCCAAAAGACCAAGAGTGCGTTAATAGTTGTGCCTCATCAGAAACAAGCTATGGA
ACAAATGAGAGTTCCCTGAATGAAAAAACACTCAAGGGACTTACTTCTATACAAAATGAA
AAAAATGTAACAGGACTTGATCTTCTTTCTTCTGTGGATGGTGGTACTTCAGATGAAATC
CAGCCGTTATATATGGGACGATGTAGTAAACCTATCTGTGATCTGATAAGTGACATGGGT
AACTTAGTTCATGCAACCAATAGTGAAGAAGATATTAAAAAATTATTGCCAGATGATTTT
AAGTCTAATGCAGATTCCTTGATTGGATTGGATTTATCTTCAGTGTCAGATACTCCCTGT
GTTTCTTCAACAGACCATGATAGTGATACTGTCAGAGAACAACAGAATGATACCAGTTCT
GAATTACAAAATAGAGAAATCGGAGGAATCAAAGAATTGGGTATAAAAGTAGATACAACA
CTTTCAGATTCCTATAATTACAGTGGAACAGAAAATTTAAAAGATAAAAAGATCTTTAAT
CAGTTAGAATCAATTGTTGATTTTAACATGTCATCTGCTTTGACTCGACAAAGTTCCAAA
ATGTTTCATGCCAAAGACAAGCTACAACACAAGAGCCAGCCATGTGGATTACTAAAAGAT
GTTGGCTTAGTAAAAGAGGAAGTAGATGTGGCAGTCATAACTGCCGCAGAATGTTTAAAA
GAAGAGGGCAAGACAAGTGCTTTGACCTGCAGCCTTCCGAAAAATGAAGATTTATGCTTA
AATGATTCAAATTCAAGAGATGAAAATTTCAAATTACCTGACTTTTCCTTTCAGGAAGAT
AAGACTGTTATAAAACAATCTGCACAAGAAGACTCAAAAAGTTTAGACCTTAAGGATAAT
GATGTAATCCAAGATTCCTCTTCAGCTTTACATGTTTCCAGTAAAGATGTGCCGTCCTCA
TTGTCCTGTCTTCCTGCGTCTGGGTCTATGTGTGGATCATTAATTGAAAGTAAAGCACGG
GGTGATTTTTTACCTCAGCATGAACATAAAGATAATATACAAGATGCAGTGACTATACAT
GAAGAAATACAGAACAGTGTTGTTCTAGGTGGGGAACCATTCAAAGAGAATGATCTTTTG
AAACAGGAAAAATGTAAAAGCATACTCCTTCAGTCATTAATTGAAGGGATGGAAGACAGA
AAGATAGATCCTGACCAGACAGTAATCAGAGCTGAGTCTTTGGATGGTGGTGACACCAGT
TCTACAGTTGTAGAATCTCAAGAGGGGCTTTCTGGCACTCATGTCCCAGAGTCTTCTGAT
TGTTGTGAAGGTTTTATTAATACTTTTTCAAGCAATGATATGGATGGGCAAGACTTAGAT
TACTTTAATATTGATGAAGGCGCAAAAAGTGGCCCACTAATTAGTGATGCTGAACTTGAT
GCCTTTCTGACAGAACAGTATCTTCAGACCACTAACATAAAGTCTTTTGAAGAAAATGTA
AATGACTCTAAATCGCAAATGAATCAGATAGATATGAAAGGCTTAGATGATGGAAACATC
AATAATATATATTTCAATGCAGAAGCAGGAGCTATTGGGGAAAGTCATGGTATTAATATA
ATTTGTGAAACAGTTGATAAACAAAATACAATAGAAAATGGCCTTTCTTTAGGAGAAAAA
AGCACTATTCCAGTTCAACAAGGGTTACCTACCAGTAAGTCTGAGATTACAAATCAATTA
TCAGTCTCTGATATTAACAGTCAATCTGTTGGAGGGGCCAGACCTAAGCAATTGTTTAGC
CTTCCATCAAGAACAAGGAGTTCAAAGGACCTGAATAAGCCAGATGTTCCAGATACAATA
GAAAGTGAACCCAGCACAGCAGATACCGTTGTTCCAATCACTTGTGCTATAGATTCTACA
GCTGATCCACAGGTTAGCTTCAACTCTAATTACATTGATATAGAAAGTAATTCTGAAGGT
GGATCTAGTTTCGTAACTGCAAATGAAGATTCTGTACCTGAAAACACTTGCAAAGAAGGC
TTGGTTTTGGGCCAGAAACAGCCTACTTGGGTTCCTGATTCAGAAGCTCCAAACTGTATG
AACTGCCAAGTCAAATTTACTTTTACCAAACGGCGACACCATTGCCGAGCATGTGGGAAA
GTATTTTGTGGTGTCTGTTGTAATAGGAAGTGTAAACTGCAATATCTAGAAAAGGAAGCA
AGAGTATGTGTAGTCTGCTATGAAACTATTAGTAAAGCTCAGGCATTTGAAAGGATGATG
AGTCCAACTGGTTCTAATCTTAAGTCTAATCATTCTGATGAATGTACTACTGTCCAGCCT
CCTCAGGAGAACCAAACATCCAGTATACCTTCACCAGCAACTTTGCCAGTCTCAGCACTT
AAACAACCAGGTGTTGAAGGACTATGTTCCAAAGAACAGAAGAGAGTATGGTTTGCAGAT
GGTATATTGCCCAATGGTGAAGTTGCAGATACAACAAAATTATCATCTGGAAGTAAAAGA
TGTTCTGAAGACTTTAGTCCTCTCTCACCTGATGTGCCTATGACAGTAAACACAGTGGAT
CATTCCCATTCTACTACAGTGGAAAAGCCAAACAATGAGACAGGAGATATTACAAGAAAT
GAGATAATTCAGAGTCCTATTTCTCAGGTTCCATCAGTGGAAAAATTGTCTATGAACACA
GGAAATGAGGGGTTACCTACTTCTGGTTCATTTACACTAGATGATGATGTTTTTGCAGAA
ACTGAAGAACCATCTAGTCCTACTGGTGTCTTAGTTAACAGCAATTTACCTATTGCTAGT
ATTTCAGATTATAGGTTACTGTGTGATATTAACAAGTATGTCTGCAATAAGATTAGTCTT
CTACCTAATGATGAGGACAGTTTGCCCCCACTTCTGGTTGCATCTGGAGAAAAGGGATCA
GTGCCTGTAGTAGAAGAACATCCATCTCATGAGCAGATCATTTTGCTTCTTGAAGGTGAA
GGCTTTCATCCTGTTACATTTGTCCTAAATGCTAATCTACTCGTGAATGTCAAATTCATA
TTTTATTCCTCAGACAAATATTGGTACTTTTCAACCAATGGATTGCATGGCTTGGGACAG
GCAGAAATTATTATTCTATTGTTATGTTTGCCAAATGAAGATACTATTCCTAAGGACATC
TTCAGACTATTTATCACCATATATAAGGATGCTCTAAAAGGAAAATACATAGAAAACTTG
GACAATATTACCTTTACTGAGAGTTTTCTCAGTAGCAAGGATCACGGAGGATTCCTGTTT
ATTACACCTACTTTTCAGAAACTTGATGATCTCTCATTACCAAGTAATCCTTTTCTTTGT
GGAATTCTTATCCAGAAGCTTGAGATTCCCTGGGCAAAGGTTTTTCCTATGCGTTTAATG
TTGAGATTGGGTGCAGAATATAAAGGTAAGTTTTAGAGTAATAAGTTAAATTGCATATTT
TCAAATATACTGAATGTAAAGACTTTCAGGGAATCATTAAAATCAATTTTAATGGATTAA
TGGAACTACTTTTGTTTTGCTATGCTTTTAAAGTTCTAAGACAACCCTCTTCTACTACCA
GGTTGGTCTAGATAACTGTGGTATGTCTTCAAGTTTCTAGATGTTCATAAGTTCTTTTAT
TACCTAAAACTTTTCTTCTTAAATTTCAGAACAAAACAGAAAACTTTCACAGTGCAGGTT
GAGTATGCCATATCTGAAATGTATTGGATCAGTTTTTTGGATTTTGGACTTTTTCAGATT
TTGGAATATTCACAGAGTACATACCAGTTGAGCATCCCAAATTCAAAATCTGAAATGCTC
CAGTGAGCATTTCTTTTGAGCATCACGTTGGCATTTGAAAAAGTTATAGACCGGGCATGG
TGGCTCACGCCTGTACTCCCGGCACTTTGGGAGGCCAAGGCGGGCGGATCACATGAGGTC
AGGAGTTCGAGACCAGCCTGGCCGGCATGGTGGAGCCCTGTCTCTACTAAAAATACAAAA
ATTGGCTGGGTGAGGTGGCGGGCGCCTGTGATTGCAGCTACTCGGGAGGCTGAGGCAGGA
GAATCGCCTGAACCCGGGAGGCAGATTTGCAGTGAGCTAAGATCATGCCTCTGCATTCCA
GCCAGGGTGACAGAGCGAGACTACGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQ ECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMG RCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDH DSDTVREQQNDTSSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIV DFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTS ALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLDLKDNDVIQDS SSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNS VVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVES QEGLSGTHVPESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQ YLQTTNIKSFEENVNDSKSQMNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICETVD KQNTIENGLSLGEKSTIPVQQGLPTSKSEITNQLSVSDINSQSVGGARPKQLFSLPSRTR SSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVSFNSNYIDIESNSEGGSSFVT ANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCRACGKVFCGVC CNRKCKLQYLEKEARVCVVCYETISKAQAFERMMSPTGSNLKSNHSDECTTVQPPQENQT SSIPSPATLPVSALKQPGVEGLCSKEQKRVWFADGILPNGEVADTTKLSSGSKRCSEDFS PLSPDVPMTVNTVDHSHSTTVEKPNNETGDITRNEIIQSPISQVPSVEKLSMNTGNEGLP TSGSFTLDDDVFAETEEPSSPTGVLVNSNLPIASISDYRLLCDINKYVCNKISLLPNDED SLPPLLVASGEKGSVPVVEEHPSHEQIILLLEGEGFHPVTFVLNANLLVNVKFIFYSSDK YWYFSTNGLHGLGQAEIIILLLCLPNEDTIPKDIFRLFITIYKDALKGKYIENLDNITFT ESFLSSKDHGGFLFITPTFQKLDDLSLPSNPFLCGILIQKLEIPWAKVFPMRLMLRLGAE YKGKF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
