DNASU Plasmid | SRC (H. sapiens )<br>In pANT7_cGST (GST-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: SRC

Gene Name: v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

Map: pANT7_cGST.doc

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pANT7_cGST

Format:

FUSION

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

generated from ORFeome entry clone (IMAGE:100073560)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00404389

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1608nts

Open reading frame: 51 to 1658

Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGGTAGCA
ACAAGAGCAAGCCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACG
TGCACGGCGCTGGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGG
CCGACGGCCACCGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGT
TCGGAGGCTTCAACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCG
GTGGAGTGACCACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGT
CCTTCAAGAAAGGCGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGACTGGTGGCTGG
CCCACTCGCTCAGCACAGGACAGACAGGCTACATCCCCAGCAACTACGTGGCGCCCTCCG
ACTCCATCCAGGCTGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAGTCAGAGCGGT
TACTGCTCAATGCAGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGTGAGACCACGA
AAGGTGCCTACTGCCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTCAACGTGAAGC
ACTACAAGATCCGCAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGCACCCAGTTCA
ACAGCCTGCAGCAGCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTGTGCCACCGCC
TCACCACCGTGTGCCCCACGTCCAAGCCGCAGACTCAGGGCCTGGCCAAGGATGCCTGGG
AGATCCCTCGGGAGTCGCTGCGGCTGGAGGTCAAGCTGGGCCAGGGCTGCTTTGGCGAGG
TGTGGATGGGGACCTGGAACGGTACCACCAGGGTGGCCATCAAAACCCTGAAGCCTGGCA
CGATGTCTCCAGAGGCCTTCCTGCAGGAGGCCCAGGTCATGAAGAAGCTGAGGCATGAGA
AGCTGGTGCAGTTGTATGCTGTGGTTTCAGAGGAGCCCATTTACATCGTCACGGAGTACA
TGAGCAAGGGGAGTTTGCTGGACTTTCTCAAGGGGGAGACAGGCAAGTACCTGCGGCTGC
CTCAGCTGGTGGACATGGCTGCTCAGATCGCCTCAGGCATGGCGTACGTGGAGCGGATGA
ACTACGTCCACCGGGACCTTCGTGCAGCCAACATCCTGGTGGGAGAGAACCTGGTGTGCA
AAGTGGCCGACTTTGGGCTGGCTCGGCTCATTGAAGACAATGAGTACACGGCGCGGCAAG
GTGCCAAATTCCCCATCAAGTGGACGGCTCCAGAAGCTGCCCTCTATGGCCGCTTCACCA
TCAAGTCGGACGTGTGGTCCTTCGGGATCCTGCTGACTGAGCTCACCACAAAGGGACGGG
TGCCCTACCCTGGGATGGTGAACCGCGAGGTGCTGGACCAGGTGGAGCGGGGCTACCGGA
TGCCCTGCCCGCCGGAGTGTCCCGAGTCCCTGCACGACCTCATGTGCCAGTGCTGGCGGA
AGGAGCCTGAGGAGCGGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAGGACTACTTCA
CGTCCACCGAGCCCCAGTACCAGCCCGGGGAGAACCTCTACCCAACTTTCTTGTACAAAG
TTGGCATTATAAGAAAGCATTGCTTATCAA

Protein sequence predicted based on nucleotide sequence

MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAE
PKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGD
WWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRES
ETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGL
CHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTL
KPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKY
LRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYT
ARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVER
GYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pANT7_cGST

Synonyms:

pANT7-cGST

Sequencing Primer:

Forward: NAP150

Reverse: NAP138R

Description:

with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial

Comments:

One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems

Size (bp):

5964

Parent Vector:

None

Properties:

Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker

Author Name:

Joshua LaBaer
Al Lau
Niroshan Ramachandran

Publications:

PMID: 15232106

Title: Self-assembling protein microarrays

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 bacterial origin of replication

3931

3249

death cassette

ccdB

ccdB death cassette present in 'empty vector' form only (replace with gene of interest)

1876

2085

primer

NAP138

NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?

2405

2384

primer

NAP150

Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?

388

408

promoter

T7 transcriptional start sequence

5940

5964

recombination site

attR1

AttR1 site for recombination in empty vector

549

565

recombination site

attR2

AttR2 site for recombination in empty vector

2236

2251

selectable marker

CmR

chloramphenicol resistance

782

1438

selectable marker

AmpR

ampicillin resistance gene

4688

4029

tag

GST

Glutathione transferase (GST) tag

2282

2950

Gene

Gene Symbol :

SRC

Symbol Nomenclature :

SRC

SYNONYM :

ASV; SRC1; c-SRC; p60-Src

Designation :

proto-oncogene c-Src|proto-oncogene tyrosine-protein kinase Src|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1

Full Nomenclature :

v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

GENEID :

6714

Locus Tag :

RP5-823N20.1

GenBank Accession :

JF432365

HGNC :

11283

MIM :

190090

Vega :

OTTHUMG00000032417

Target GenBank:

JF432365

Reference Sequence Alignment

  

HsCD00404389      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGGTAGCA
NM_198291.2       --------------------------------------------------ATGGGTAGCA
NM_005417.4       --------------------------------------------------ATGGGTAGCA
                                                                    **********

HsCD00404389      ACAAGAGCAAGCCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACG
NM_198291.2       ACAAGAGCAAGCCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACG
NM_005417.4       ACAAGAGCAAGCCCAAGGATGCCAGCCAGCGGCGCCGCAGCCTGGAGCCCGCCGAGAACG
                  ************************************************************

HsCD00404389      TGCACGGCGCTGGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGG
NM_198291.2       TGCACGGCGCTGGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGG
NM_005417.4       TGCACGGCGCTGGCGGGGGCGCTTTCCCCGCCTCGCAGACCCCCAGCAAGCCAGCCTCGG
                  ************************************************************

HsCD00404389      CCGACGGCCACCGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGT
NM_198291.2       CCGACGGCCACCGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGT
NM_005417.4       CCGACGGCCACCGCGGCCCCAGCGCGGCCTTCGCCCCCGCGGCCGCCGAGCCCAAGCTGT
                  ************************************************************

HsCD00404389      TCGGAGGCTTCAACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCG
NM_198291.2       TCGGAGGCTTCAACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCG
NM_005417.4       TCGGAGGCTTCAACTCCTCGGACACCGTCACCTCCCCGCAGAGGGCGGGCCCGCTGGCCG
                  ************************************************************

HsCD00404389      GTGGAGTGACCACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGT
NM_198291.2       GTGGAGTGACCACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGT
NM_005417.4       GTGGAGTGACCACCTTTGTGGCCCTCTATGACTATGAGTCTAGGACGGAGACAGACCTGT
                  ************************************************************

HsCD00404389      CCTTCAAGAAAGGCGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGACTGGTGGCTGG
NM_198291.2       CCTTCAAGAAAGGCGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGACTGGTGGCTGG
NM_005417.4       CCTTCAAGAAAGGCGAGCGGCTCCAGATTGTCAACAACACAGAGGGAGACTGGTGGCTGG
                  ************************************************************

HsCD00404389      CCCACTCGCTCAGCACAGGACAGACAGGCTACATCCCCAGCAACTACGTGGCGCCCTCCG
NM_198291.2       CCCACTCGCTCAGCACAGGACAGACAGGCTACATCCCCAGCAACTACGTGGCGCCCTCCG
NM_005417.4       CCCACTCGCTCAGCACAGGACAGACAGGCTACATCCCCAGCAACTACGTGGCGCCCTCCG
                  ************************************************************

HsCD00404389      ACTCCATCCAGGCTGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAGTCAGAGCGGT
NM_198291.2       ACTCCATCCAGGCTGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAGTCAGAGCGGT
NM_005417.4       ACTCCATCCAGGCTGAGGAGTGGTATTTTGGCAAGATCACCAGACGGGAGTCAGAGCGGT
                  ************************************************************

HsCD00404389      TACTGCTCAATGCAGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGTGAGACCACGA
NM_198291.2       TACTGCTCAATGCAGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGTGAGACCACGA
NM_005417.4       TACTGCTCAATGCAGAGAACCCGAGAGGGACCTTCCTCGTGCGAGAAAGTGAGACCACGA
                  ************************************************************

HsCD00404389      AAGGTGCCTACTGCCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTCAACGTGAAGC
NM_198291.2       AAGGTGCCTACTGCCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTCAACGTGAAGC
NM_005417.4       AAGGTGCCTACTGCCTCTCAGTGTCTGACTTCGACAACGCCAAGGGCCTCAACGTGAAGC
                  ************************************************************

HsCD00404389      ACTACAAGATCCGCAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGCACCCAGTTCA
NM_198291.2       ACTACAAGATCCGCAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGCACCCAGTTCA
NM_005417.4       ACTACAAGATCCGCAAGCTGGACAGCGGCGGCTTCTACATCACCTCCCGCACCCAGTTCA
                  ************************************************************

HsCD00404389      ACAGCCTGCAGCAGCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTGTGCCACCGCC
NM_198291.2       ACAGCCTGCAGCAGCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTGTGCCACCGCC
NM_005417.4       ACAGCCTGCAGCAGCTGGTGGCCTACTACTCCAAACACGCCGATGGCCTGTGCCACCGCC
                  ************************************************************

HsCD00404389      TCACCACCGTGTGCCCCACGTCCAAGCCGCAGACTCAGGGCCTGGCCAAGGATGCCTGGG
NM_198291.2       TCACCACCGTGTGCCCCACGTCCAAGCCGCAGACTCAGGGCCTGGCCAAGGATGCCTGGG
NM_005417.4       TCACCACCGTGTGCCCCACGTCCAAGCCGCAGACTCAGGGCCTGGCCAAGGATGCCTGGG
                  ************************************************************

HsCD00404389      AGATCCCTCGGGAGTCGCTGCGGCTGGAGGTCAAGCTGGGCCAGGGCTGCTTTGGCGAGG
NM_198291.2       AGATCCCTCGGGAGTCGCTGCGGCTGGAGGTCAAGCTGGGCCAGGGCTGCTTTGGCGAGG
NM_005417.4       AGATCCCTCGGGAGTCGCTGCGGCTGGAGGTCAAGCTGGGCCAGGGCTGCTTTGGCGAGG
                  ************************************************************

HsCD00404389      TGTGGATGGGGACCTGGAACGGTACCACCAGGGTGGCCATCAAAACCCTGAAGCCTGGCA
NM_198291.2       TGTGGATGGGGACCTGGAACGGTACCACCAGGGTGGCCATCAAAACCCTGAAGCCTGGCA
NM_005417.4       TGTGGATGGGGACCTGGAACGGTACCACCAGGGTGGCCATCAAAACCCTGAAGCCTGGCA
                  ************************************************************

HsCD00404389      CGATGTCTCCAGAGGCCTTCCTGCAGGAGGCCCAGGTCATGAAGAAGCTGAGGCATGAGA
NM_198291.2       CGATGTCTCCAGAGGCCTTCCTGCAGGAGGCCCAGGTCATGAAGAAGCTGAGGCATGAGA
NM_005417.4       CGATGTCTCCAGAGGCCTTCCTGCAGGAGGCCCAGGTCATGAAGAAGCTGAGGCATGAGA
                  ************************************************************

HsCD00404389      AGCTGGTGCAGTTGTATGCTGTGGTTTCAGAGGAGCCCATTTACATCGTCACGGAGTACA
NM_198291.2       AGCTGGTGCAGTTGTATGCTGTGGTTTCAGAGGAGCCCATTTACATCGTCACGGAGTACA
NM_005417.4       AGCTGGTGCAGTTGTATGCTGTGGTTTCAGAGGAGCCCATTTACATCGTCACGGAGTACA
                  ************************************************************

HsCD00404389      TGAGCAAGGGGAGTTTGCTGGACTTTCTCAAGGGGGAGACAGGCAAGTACCTGCGGCTGC
NM_198291.2       TGAGCAAGGGGAGTTTGCTGGACTTTCTCAAGGGGGAGACAGGCAAGTACCTGCGGCTGC
NM_005417.4       TGAGCAAGGGGAGTTTGCTGGACTTTCTCAAGGGGGAGACAGGCAAGTACCTGCGGCTGC
                  ************************************************************

HsCD00404389      CTCAGCTGGTGGACATGGCTGCTCAGATCGCCTCAGGCATGGCGTACGTGGAGCGGATGA
NM_198291.2       CTCAGCTGGTGGACATGGCTGCTCAGATCGCCTCAGGCATGGCGTACGTGGAGCGGATGA
NM_005417.4       CTCAGCTGGTGGACATGGCTGCTCAGATCGCCTCAGGCATGGCGTACGTGGAGCGGATGA
                  ************************************************************

HsCD00404389      ACTACGTCCACCGGGACCTTCGTGCAGCCAACATCCTGGTGGGAGAGAACCTGGTGTGCA
NM_198291.2       ACTACGTCCACCGGGACCTTCGTGCAGCCAACATCCTGGTGGGAGAGAACCTGGTGTGCA
NM_005417.4       ACTACGTCCACCGGGACCTTCGTGCAGCCAACATCCTGGTGGGAGAGAACCTGGTGTGCA
                  ************************************************************

HsCD00404389      AAGTGGCCGACTTTGGGCTGGCTCGGCTCATTGAAGACAATGAGTACACGGCGCGGCAAG
NM_198291.2       AAGTGGCCGACTTTGGGCTGGCTCGGCTCATTGAAGACAATGAGTACACGGCGCGGCAAG
NM_005417.4       AAGTGGCCGACTTTGGGCTGGCTCGGCTCATTGAAGACAATGAGTACACGGCGCGGCAAG
                  ************************************************************

HsCD00404389      GTGCCAAATTCCCCATCAAGTGGACGGCTCCAGAAGCTGCCCTCTATGGCCGCTTCACCA
NM_198291.2       GTGCCAAATTCCCCATCAAGTGGACGGCTCCAGAAGCTGCCCTCTATGGCCGCTTCACCA
NM_005417.4       GTGCCAAATTCCCCATCAAGTGGACGGCTCCAGAAGCTGCCCTCTATGGCCGCTTCACCA
                  ************************************************************

HsCD00404389      TCAAGTCGGACGTGTGGTCCTTCGGGATCCTGCTGACTGAGCTCACCACAAAGGGACGGG
NM_198291.2       TCAAGTCGGACGTGTGGTCCTTCGGGATCCTGCTGACTGAGCTCACCACAAAGGGACGGG
NM_005417.4       TCAAGTCGGACGTGTGGTCCTTCGGGATCCTGCTGACTGAGCTCACCACAAAGGGACGGG
                  ************************************************************

HsCD00404389      TGCCCTACCCTGGGATGGTGAACCGCGAGGTGCTGGACCAGGTGGAGCGGGGCTACCGGA
NM_198291.2       TGCCCTACCCTGGGATGGTGAACCGCGAGGTGCTGGACCAGGTGGAGCGGGGCTACCGGA
NM_005417.4       TGCCCTACCCTGGGATGGTGAACCGCGAGGTGCTGGACCAGGTGGAGCGGGGCTACCGGA
                  ************************************************************

HsCD00404389      TGCCCTGCCCGCCGGAGTGTCCCGAGTCCCTGCACGACCTCATGTGCCAGTGCTGGCGGA
NM_198291.2       TGCCCTGCCCGCCGGAGTGTCCCGAGTCCCTGCACGACCTCATGTGCCAGTGCTGGCGGA
NM_005417.4       TGCCCTGCCCGCCGGAGTGTCCCGAGTCCCTGCACGACCTCATGTGCCAGTGCTGGCGGA
                  ************************************************************

HsCD00404389      AGGAGCCTGAGGAGCGGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAGGACTACTTCA
NM_198291.2       AGGAGCCTGAGGAGCGGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAGGACTACTTCA
NM_005417.4       AGGAGCCTGAGGAGCGGCCCACCTTCGAGTACCTGCAGGCCTTCCTGGAGGACTACTTCA
                  ************************************************************

HsCD00404389      CGTCCACCGAGCCCCAGTACCAGCCCGGGGAGAACCTCTACCCAACTTTCTTGTACAAAG
NM_198291.2       CGTCCACCGAGCCCCAGTACCAGCCCGGGGAGAACCTCTAG-------------------
NM_005417.4       CGTCCACCGAGCCCCAGTACCAGCCCGGGGAGAACCTCTAG-------------------
                  ****************************************                    

HsCD00404389      TTGGCATTATAAGAAAGCATTGCTTATCAA
NM_198291.2       ------------------------------
NM_005417.4       ------------------------------

Pathways

NCI :

Alpha-synuclein signaling

NCI :

Alpha4 beta1 integrin signaling events

NCI :

Alpha9 beta1 integrin signaling events

NCI :

amb2 Integrin signaling

NCI :

Arf6 signaling events

NCI :

Atypical NF-kappaB pathway

NCI :

CDC42 signaling events

NCI :

Class I PI3K signaling events

NCI :

Class I PI3K signaling events mediated by Akt

NCI :

CXCR3-mediated signaling events

NCI :

CXCR4-mediated signaling events

NCI :

E-cadherin signaling in keratinocytes

NCI :

E-cadherin signaling in the nascent adherens junction

NCI :

EGF receptor (ErbB1) signaling pathway

NCI :

Endothelins

NCI :

EPHA forward signaling

NCI :

EPHA2 forward signaling

NCI :

EPHB forward signaling

NCI :

Ephrin B reverse signaling

NCI :

ErbB1 downstream signaling

NCI :

ErbB2/ErbB3 signaling events

NCI :

FAS (CD95) signaling pathway

NCI :

FGF signaling pathway

NCI :

Glypican 1 network

NCI :

Integrins in angiogenesis

NCI :

Internalization of ErbB1

NCI :

LPA receptor mediated events

NCI :

Nectin adhesion pathway

NCI :

Netrin-mediated signaling events

NCI :

Nongenotropic Androgen signaling

NCI :

PDGFR-beta signaling pathway

NCI :

Plasma membrane estrogen receptor signaling

NCI :

Posttranslational regulation of adherens junction stability and dissassembly

NCI :

Regulation of Androgen receptor activity

NCI :

Regulation of p38-alpha and p38-beta

NCI :

S1P3 pathway

NCI :

Signaling events mediated by focal adhesion kinase

NCI :

Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)

NCI :

Signaling events mediated by PRL

NCI :

Signaling events mediated by PTP1B

NCI :

Signaling events mediated by TCPTP

NCI :

Signaling events mediated by VEGFR1 and VEGFR2

NCI :

Signaling events regulated by Ret tyrosine kinase

NCI :

Syndecan-2-mediated signaling events

NCI :

Syndecan-3-mediated signaling events

NCI :

Thromboxane A2 receptor signaling

NCI :

Trk receptor signaling mediated by PI3K and PLC-gamma

NCI :

Urokinase-type plasminogen activator (uPA) and uPAR-mediated signaling

Panther :

Angiogenesis

Panther :

Cadherin signaling pathway

Panther :

Integrin signalling pathway

Panther :

Parkinson disease

Reactome :

Adaptive Immune System

Reactome :

ADP signalling through P2Y purinoceptor 1

Reactome :

Axon guidance

Reactome :

c-src mediated regulation of Cx43 function and closure of gap junctions

Reactome :

CD28 co-stimulation

Reactome :

Cell surface interactions at the vascular wall

Reactome :

Cell-Cell communication

Reactome :

Costimulation by the CD28 family

Reactome :

CTLA4 inhibitory signaling

Reactome :

DCC mediated attractive signaling

Reactome :

Developmental Biology

Reactome :

Disease

Reactome :

Downstream signal transduction

Reactome :

Fcgamma receptor (FCGR) dependent phagocytosis

Reactome :

FCGR activation

Reactome :

GAB1 signalosome

Reactome :

Gap junction trafficking and regulation

Reactome :

GP1b-IX-V activation signalling

Reactome :

GRB2:SOS provides linkage to MAPK signaling for Integrins

Reactome :

Hemostasis

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Integrin alphaIIb beta3 signaling

Reactome :

L1CAM interactions

Reactome :

Membrane Trafficking

Reactome :

NCAM signaling for neurite out-growth

Reactome :

Negative regulation of FGFR signaling

Reactome :

Netrin mediated repulsion signals

Reactome :

Netrin-1 signaling

Reactome :

NGF signalling via TRKA from the plasma membrane

Reactome :

p130Cas linkage to MAPK signaling for integrins

Reactome :

p38MAPK events

Reactome :

PECAM1 interactions

Reactome :

Platelet activation, signaling and aggregation

Reactome :

Platelet Aggregation (Plug Formation)

Reactome :

Recycling pathway of L1

Reactome :

Regulation of gap junction activity

Reactome :

Regulation of KIT signaling

Reactome :

Signal amplification

Reactome :

Signal regulatory protein (SIRP) family interactions

Reactome :

Signal Transduction

Reactome :

Signaling by EGFR

Reactome :

Signaling by EGFR in Cancer

Reactome :

Signaling by ERBB2

Reactome :

Signaling by FGFR

Reactome :

Signaling by FGFR in disease

Reactome :

Signaling by PDGF

Reactome :

Signaling by SCF-KIT

Reactome :

Signalling by NGF

Reactome :

Signalling to ERKs

Reactome :

Signalling to RAS

Reactome :

Spry regulation of FGF signaling

Reactome :

Thrombin signalling through proteinase activated receptors (PARs)

WikiPathway :

Alpha 6 Beta 4 signaling pathway

WikiPathway :

Androgen receptor signaling pathway

WikiPathway :

Angiogenesis

WikiPathway :

angiogenesis overview

WikiPathway :

EPO Receptor Signaling

WikiPathway :

ErbB signaling pathway

WikiPathway :

Focal Adhesion

WikiPathway :

FSH signaling pathway

WikiPathway :

IL-3 Signaling Pathway

WikiPathway :

Integrin-mediated cell adhesion

WikiPathway :

Kit receptor signaling pathway

WikiPathway :

Leptin signaling pathway

WikiPathway :

Notch Signaling Pathway

WikiPathway :

Prolactin Signaling Pathway

WikiPathway :

RANKL/RANK Signaling Pathway

WikiPathway :

Senescence and Autophagy

WikiPathway :

Signaling of Hepatocyte Growth Factor Receptor

WikiPathway :

TGF beta Signaling Pathway

WikiPathway :

TSH signaling pathway

Protein

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

SMART domain :

SH2 : Src homology 2 domains

SMART domain :

SH3 : Src homology 3 domains

HPRD :

01819

Clone

Cloning Information :

579326

Organism

Species Specific ID:

6714

Genome

Chromosome :

Map Location :

20q12-q13

Ensembl :

ENSG00000197122

Reagents

Labome :

Src-antibody

SRC (H. sapiens )In pANT7_cGST (GST-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

Reagents

SRC (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)