HRM2_25430 (D. autotrophicum HRM2 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  HRM2_25430
Gene Name:  putative lipopolysaccharide N-acetyl-glycoaminyltransferase
Original Clone ID:
BGT12_T01a
Keyword:
full-length CDS
Species:
Desulforapulum autotrophicum HRM2
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
DaCD00405495
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 873nts
Open reading frame: 1 to 873

ATTCGTAAATTAAAAAAAATACTATTTAGATATTTAAAAACCGGAAAGCTGGTTTTTATC
GGAAAGCCTGTATACAAAGCGATTATAACTATTGAACTTTTATTTCATCGTTTGGTTGAT
TTGTGTGCTCGAACCGGTTCTGGTTCACAGGATAATCTTAATTCTGATTTAACTGTCCTT
GTCAAGACCTTTGAAAGACCTCGAACGGTAAAACGGCTTATTAAAAGCATAAAAAGGTTT
TATCCCAACCTTAGGATTATTCTTGTTGACGATAGTAAATATCCTGTTCAAATCAATGGA
ATAGAAACTGTGGTTATGCCATTTGACAGCGGTATATCCGCCGGAAGGAATAAAGCGTTA
TCACTGGTTAAAACAGAATTTGTTTTAGTGCTGGACGATGATTTTGTTTTTTTTAGAAAA
ACCGACCTTGTCAATGCCCTTAAAAGAATTAAAGCCAATCCAAATATTGATATTTTAGGG
GGCGAAGTCATAGATCTTCCATTTTACAAAACCTTTGACTACTATACAGCTCCCATATTT
GCAACAGTGGCTCAACCGACAATGCCAACTGGATCATTAATTGATGGTATGCCTGTGCTT
GACAAGGTTGCAAATTTTTATATCGGCAGGACCCATGCTATAAAAAAAATCAAATGGGAT
AACCTATTAAAAAGGTTAGATCATGCTGACTTTTTTACCAGGGCCAAAGGCGTTTTGACA
TCTGTTTTAACAGATGAGTTTAAATGTCTTCATGCCAGAACCATTTATAATAGAACTTAT
ATGAGTTTCCGAAACAATCTAAAAAGAGATCAGATATACTTGTTTCTAAAATACAACCAT
GAATCTAATATTAAAAACCCTCAGATGCCATAG

Protein sequence predicted based on nucleotide sequence

IRKLKKILFRYLKTGKLVFIGKPVYKAIITIELLFHRLVDLCARTGSGSQDNLNSDLTVL
VKTFERPRTVKRLIKSIKRFYPNLRIILVDDSKYPVQINGIETVVMPFDSGISAGRNKAL
SLVKTEFVLVLDDDFVFFRKTDLVNALKRIKANPNIDILGGEVIDLPFYKTFDYYTAPIF
ATVAQPTMPTGSLIDGMPVLDKVANFYIGRTHAIKKIKWDNLLKRLDHADFFTRAKGVLT
SVLTDEFKCLHARTIYNRTYMSFRNNLKRDQIYLFLKYNHESNIKNPQMP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable