BT_1294 (B. thetaiotaomicron VPI-5482 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_1294
Gene Name:  glycogen [starch] synthase, glycosyltransferase
Original Clone ID:
BGT03_T01c
Keyword:
full-length CDS
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BtCD00405500
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1659nts
Open reading frame: 1 to 1659

GTAAAAGATTTATTAACCCCCGATTATATCTTCGAGTCCAGCTGGGAAGTATGTAATAAA
GTGGGAGGGATATACACCGTATTGTCGACACGGGCGAATACATTGCAGGAAAAATTCCGC
GACAGAATTTTTTTCATAGGTCCTGATGTGTGGCAAGGAAAAGAGAACCCTCTATTCATC
GAGTCGGATAATCTGTGCGCTGACTGGAAAAAGCACGCATTCAAAAACGATCATCTTTCC
GTCCGTGTAGGACGATGGAATATACCCGGTGAACCCATTGTCATCCTTGTTGATTTCCAA
CCTTTTTTTGAACAGAAGAACGAAATTTACACAGAAATGTGGAATCGTTTTCAAGTAGAC
TCGTTGCACGCTTATGGCGATTACGACGAGGCTTCGATGTTTTCGTATGCTGCCGGAAAA
GTAGTAGAGAGTTTCTACCGCTACAACCTGACAGAAACGGATAAGGTGGTATATCAGGCA
CATGAATGGATGACCGGAATGGGAGCACTTTATGTACAGGAAGCTGTCCCCGAAGTGGCT
ACTATTTTTACAACCCACGCTACTTCCATCGGACGTTCGATAGCCGGCAATCATAAGCCG
CTGTATGACTATCTGTTTGCTTATAACGGTGACCAGATGGCGCAGGAACTGAATATGCAG
TCAAAGCACTCTATTGAGAAGCAGACGGCGCATTATGTAGACTGTTTTACGACAGTAAGT
GAAATCACGAATAACGAGTGCAAGGAATTGCTCGACAAACCGGCAGACGTAGTACTGATG
AACGGTTTCGAAGACGATTTCGTACCTAAAGGCAGTACATTTACCGGAAAACGTAAACGT
GCCCGCTCATTGATGTTGAACGTAGCCAATAAATTGTTGGGAACAAATCTGGATGACGAT
ACGTTAATTATCGGTACCAGTGGTCGATATGAATTCAAGAACAAGGGTATTGACGTATTC
CTGGAGTCATTGAACCGTCTGAACAGGGATAAAAAGCTCCATAAGAACGTATTGGCGTTT
ATCAATGTCCCCGGTTGGGTAGGAGATCCCCGTGAAGACTTGCAGGAACGTCTGAAAAGC
AAAGAAAAATTTGATACACCGCTTGAAGTTCCTTTCATCACTCATTGGCTGCACAATATG
ACGCATGACCAAGTGCTGGATATGCTGAAATATCTGGGGATGGGCAACCGTCCCGATGAT
AAAGTAAAGGTGATTTTTGTGCCTTGCTATCTGGACGGGCGTGATGGTATCCTGAATAAA
GAGTATTATGATATATTGCTGGGACAGGATTTGAGTGTATATGCTTCCTATTACGAACCG
TGGGGATATACTCCGCTGGAAAGTGTAGCGTTCCGTGTGCCTACCATTACTACTGATCTG
GCAGGTTTTGGCCTTTGGGTGAACAGCCTGAAGAATCAGCATGGCATCAATGATGGAGTG
GAAGTGCTGCATCGTTCGGACTATAATGATTCGGAAGTGGCGGATGGCATCAAAGATACG
ATTACGCTGTTTGCAGATAAGTCGGAAAAAGAAGTGAAGGAGATCCGTAAGCATGCGGCA
GATGTTGCAGAGCAGGCATTGTGGAAACACTTCATTCAATATTATTACGAGGCTTATGAT
ATTGCCTTGCGCAATGCCATGAAGCGTCAGTTAGGCTAG

Protein sequence predicted based on nucleotide sequence

VKDLLTPDYIFESSWEVCNKVGGIYTVLSTRANTLQEKFRDRIFFIGPDVWQGKENPLFI
ESDNLCADWKKHAFKNDHLSVRVGRWNIPGEPIVILVDFQPFFEQKNEIYTEMWNRFQVD
SLHAYGDYDEASMFSYAAGKVVESFYRYNLTETDKVVYQAHEWMTGMGALYVQEAVPEVA
TIFTTHATSIGRSIAGNHKPLYDYLFAYNGDQMAQELNMQSKHSIEKQTAHYVDCFTTVS
EITNNECKELLDKPADVVLMNGFEDDFVPKGSTFTGKRKRARSLMLNVANKLLGTNLDDD
TLIIGTSGRYEFKNKGIDVFLESLNRLNRDKKLHKNVLAFINVPGWVGDPREDLQERLKS
KEKFDTPLEVPFITHWLHNMTHDQVLDMLKYLGMGNRPDDKVKVIFVPCYLDGRDGILNK
EYYDILLGQDLSVYASYYEPWGYTPLESVAFRVPTITTDLAGFGLWVNSLKNQHGINDGV
EVLHRSDYNDSEVADGIKDTITLFADKSEKEVKEIRKHAADVAEQALWKHFIQYYYEAYD
IALRNAMKRQLG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable