DICTH_1429 (D. thermophilum H-6-12 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  DICTH_1429
Gene Name:  beta-D-glucuronidase
Original Clone ID:
BGH02_B01c
Keyword:
full-length CDS
Species:
Dictyoglomus thermophilum H-6-12
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
DtCD00405501
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1734nts
Open reading frame: 1 to 1734

CTTTATCCTAAAGAGAGTGAAACAAGAGAGGTAAAGGATTTGTCAGGAGTGTGGGAGTTT
AGAACCGAATCTGATAAAAATTATATCCTCATGCCAGTTCCTGCAAGTTTTAACGACATC
ACACAAGATATTAATTTAAGAGATTATGTTGGCAGAGTATATTATAAAAAATCCTTCTTT
ATACCGGTTTATTGGAAAGAAAGAAATATTTTTTTGAGGGTGGGAGCAGCAGCTCATTTT
TCTGAGGTTTATGTAAATGGTAATTTAGTGACTAAACACAAAGGAGGATTTCTTCCTTTC
GAGGCAGAGATCTCTAAATTTGTAAATTATGGACAAGAAAATATTATAGAAATTATGGTA
GACAACACTTTAACTTGGGATGTTCTTCCTCCTGGAGAATTAAAAGTTATTGAGGACGAA
ATGCATCCTAAAGGATATAAGGTTTTGAATTATTATTTTGATTTTTTTAATTACTCTGGA
ATTCACAGGCCTGTGGTAATATATACCACTCCTAAGGTATATATTAAAGATTTTTCTGTT
ATTACTGAACTAAGTAATAATTCTGCATTGGTAAAGTACAGTATTGAAAGTGAAGGTAAT
AATTTTCAAGTTATTTTAAGAGATAAAGATAAAAATATAGTAGCAGAGAATTTTGGTAAA
TCAGGAGTTTTAGAGGTAAAAAATCCAAAATTATGGGAGCCTGGAAATCCTTATCTATAT
AATTTAGAGATTAAACTTTTGGAAAAAGACAACTTTGATATTTATAGAATGGACATAGGA
ATAAGGACGGTAAGAGTAGAGGGAAAACAGTTTCTTATAAATGAGAAACCATTTTACTTT
AAGGGTTTTGGTAAGCATGAGGACTCAGATATAAGGGGAAAAGGTTTAGATCAAGTTATT
AACATTAAAGATTTTAATTTATTGAAATGGATAGGGGCAAACTCTTTTAGAACCTCTCAC
TATCCCTATAGCGAGGAGATACTTTTCCTTGCTGACCAATATGGGATAGCTGTTATTGAG
GAAGCTCCAGCTGTTGGGTTAAATTTATGGAACCGTAATGAAAAAGTTTTTACTGAAGGA
AGGGTTGATGGAAAGACATTAGAGCATCATTTAGAAATTATGAGGGAACTTATAGCGAGG
GATAAAAATCATCCGAGTGTAATTATGTGGAGCGTAGCTAATGAGGCGGCAACTTACGAA
GATGGAGCAGAGGAATATTTTAGGAGAGTTATAGAGGAAACAAGAAGGCTCGATCCTACA
AGACCAATAACCATAGTAGAAAATACTAAGGCAAGTGAGACGAAAGTAAGTAAATATGTT
GATGTGATATGTGTTAATAGATACTATTCATGGTATACCGATTCGGGAGATTTAAGTGTT
ATTGAATATCAACTTGAAAGAGATCTTAGAGAGTGGTATGAATTATACAGAAAGCCGATA
ATTCTCTCTGAGTTCGGGGCAGATGCTATTTCTGGATTTCATTCTGATCCTCCCTTAATG
TTTACAGAGGAGTATCAACAAGAGATGATTAAAAGATTTGTGGGAGTTCTTGACAGGCTT
GATTTTGTTGTGGGAGAGCATATTTGGAATTTTGCTGATTTTATGACGAAACAGAGTATA
ACGAGGGTAGTGGGCAATAAGAAAGGGGTTTTTACAAGGAATAGACAGCCTAAAATGGTG
GCTCATTTTTTGAAAGAGAGATGGAGTAAATTACCTGATTTTTGGGAAAAATAG

Protein sequence predicted based on nucleotide sequence

LYPKESETREVKDLSGVWEFRTESDKNYILMPVPASFNDITQDINLRDYVGRVYYKKSFF
IPVYWKERNIFLRVGAAAHFSEVYVNGNLVTKHKGGFLPFEAEISKFVNYGQENIIEIMV
DNTLTWDVLPPGELKVIEDEMHPKGYKVLNYYFDFFNYSGIHRPVVIYTTPKVYIKDFSV
ITELSNNSALVKYSIESEGNNFQVILRDKDKNIVAENFGKSGVLEVKNPKLWEPGNPYLY
NLEIKLLEKDNFDIYRMDIGIRTVRVEGKQFLINEKPFYFKGFGKHEDSDIRGKGLDQVI
NIKDFNLLKWIGANSFRTSHYPYSEEILFLADQYGIAVIEEAPAVGLNLWNRNEKVFTEG
RVDGKTLEHHLEIMRELIARDKNHPSVIMWSVANEAATYEDGAEEYFRRVIEETRRLDPT
RPITIVENTKASETKVSKYVDVICVNRYYSWYTDSGDLSVIEYQLERDLREWYELYRKPI
ILSEFGADAISGFHSDPPLMFTEEYQQEMIKRFVGVLDRLDFVVGEHIWNFADFMTKQSI
TRVVGNKKGVFTRNRQPKMVAHFLKERWSKLPDFWEK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable