BT_0771 (B. thetaiotaomicron VPI-5482 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_0771
Gene Name:  1,4-alpha-glucan branching enzyme
Original Clone ID:
BGH13_B05a2
Keyword:
partial CDS
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BtCD00405504
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 990nts
Open reading frame: 1 to 990

GTGCAGGACGAGCAGACCAAGATATTCAGCGCACAAGTCTGGGCACCGGAAAAGCCATAT
AAATTCAAGAAAAAGACATTTAAGCCTACTACGAATCCTTTGTTGATCTATGAATGTCAT
ATTGGAATGGCACAACAGGAGGAAAAAGTAGGCACATACAATGAATTCCGCGAAAAAACA
CTTCCACGCATAGCACAGGAAGGCTATAACTGTATTCAGATTATGGCCATTCAGGAGCAT
CCTTATTATGGTAGTTTTGGCTATCATGTATCCAGTTTCTTTGCTGCTTCCTCCCGCTTT
GGTACGCCTGACGAACTGAAAGCGCTGATTGATGCTGCTCATGAGATGGGGATTGCCGTT
ATTATGGATATTGTCCATTCTCATGCGGTGAAAAACGAAGTGGAAGGTTTGGGCAACTTT
GCCGGTGATCCGAACCAGTATTTCTATCCGGCTCCTCGTCGTGAGCATCCGGCATGGGAT
TCACTATGCTTTGATTATGGTAAAAATGAAGTGATCCATTTCTTATTGTCCAACTGTAAA
TACTGGCTGGAAGAATATAAGTTTGACGGATTCCGTTTTGACGGTGTGACGTCTATGTTG
TATTATAGCCACGGGTTGGGAGAAGCATTCTGTAATTATGGAGATTACTTCAATGGTCAT
CAGGACGGCAATGCCATTTGCTACCTGACATTGGCGAATGAGTTGATTCATCAGGTCAAT
CCGAAGGCTATCTCTATTGCTGAAGAAGTTTCCGGTATGCCGGGACTTGCCGCCAAAGTA
GAAGATGGCGGTTACGGATTTGATTACCGTATGGCTATGAACATCCCTGATTACTGGATC
AAGACTATCAAGGAAAAGATTGATGAGGACTGGAAACCTTCCAGTATGTTCTGGGAAGTA
ACCAACCGTCGTCAGGATGAAAAGACGATCTCTTATGCGGAAAGTCATGATCAGGCATTG
GTAGGAGACAAGACTATTATTTTCCGTTAG

Protein sequence predicted based on nucleotide sequence

VQDEQTKIFSAQVWAPEKPYKFKKKTFKPTTNPLLIYECHIGMAQQEEKVGTYNEFREKT
LPRIAQEGYNCIQIMAIQEHPYYGSFGYHVSSFFAASSRFGTPDELKALIDAAHEMGIAV
IMDIVHSHAVKNEVEGLGNFAGDPNQYFYPAPRREHPAWDSLCFDYGKNEVIHFLLSNCK
YWLEEYKFDGFRFDGVTSMLYYSHGLGEAFCNYGDYFNGHQDGNAICYLTLANELIHQVN
PKAISIAEEVSGMPGLAAKVEDGGYGFDYRMAMNIPDYWIKTIKEKIDEDWKPSSMFWEV
TNRRQDEKTISYAESHDQALVGDKTIIFR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable