BT_3862 (B. thetaiotaomicron VPI-5482 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_3862
Gene Name:  endo-alpha-mannosidase
Original Clone ID:
BGH99_B01a
Keyword:
partial CDS
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BtCD00405511
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1095nts
Open reading frame: 1 to 1095

AACGGTAACAAAAAGAAAATGAACGGTGAACACGATTTGGATGCGGCAAACATTACGTTG
GATGACCATACGATCAGTTTTTATTATAATTGGTATGGAAATCCGTCAGTGGATGGAGAA
ATGAAGCACTGGATGCACCCGATAGCCCTTGCTCCGGGACATTCGGGAGATGTCGGTGCC
ATATCCGGACTTAATGATGACATCGCCTGTAATTTTTATCCGGAGCTCGGAACGTACAGC
AGCAATGATCCTGAAATCATTCGGAAACATATCCGGATGCATATAAAAGCGAATGTCGGT
GTACTGTCTGTCACTTGGTGGGGAGAAAGCGATTATGGCAACCAAAGTGTGTCTCTCCTG
CTGGATGAGGCTGCAAAAGTAGGGGCAAAGGTGTGCTTTCATATAGAGCCTTTTAATGGA
CGCAGCCCGCAAACGGTAAGGGAGAATATTCAATATATAGTGGATACTTATGGTGATCAT
CCGGCTTTTTACCGTACGCACGGCAAACCTCTTTTCTTTATCTATGATTCTTATCTGATC
AAACCTGCCGAGTGGGCGAAGTTGTTTGCTGCCGGGGGAGAGATAAGTGTGCGTAATACC
AAGTACGACGGTCTTTTTATTGGTCTGACATTGAAGGAAAGCGAGTTGCCCGACATTGAG
ACAGCGTGCATGGATGGCTTTTACACTTACTTTGCCGCAACAGGTTTCACAAATGCTTCT
ACTCCGGCCAACTGGAAATCCATGCAGCAATGGGCAAAGGCACATAATAAATTGTTTATT
CCGAGTGTCGGTCCGGGATATATTGATACCCGGATTCGTCCTTGGAACGGAAGTACCACC
CGAGACCGTGAGAATGGAAAATATTACGATGATATGTATAAAGCTGCCATAGAAAGCGGT
GCTTCTTATATTTCGATTACGTCTTTCAATGAATGGCATGAAGGAACTCAGATAGAGCCG
GCTGTCTCAAAGAAGTGCGATGCTTTTGAATATTTGGATTATAAACCATTGGCTGATGAT
TACTATTTGATAAGAACTGCCTATTGGGTAGATGAATTCCGGAAAGCAAGATCTGCTTCG
GAAGATGTTCAATAG

Protein sequence predicted based on nucleotide sequence

NGNKKKMNGEHDLDAANITLDDHTISFYYNWYGNPSVDGEMKHWMHPIALAPGHSGDVGA
ISGLNDDIACNFYPELGTYSSNDPEIIRKHIRMHIKANVGVLSVTWWGESDYGNQSVSLL
LDEAAKVGAKVCFHIEPFNGRSPQTVRENIQYIVDTYGDHPAFYRTHGKPLFFIYDSYLI
KPAEWAKLFAAGGEISVRNTKYDGLFIGLTLKESELPDIETACMDGFYTYFAATGFTNAS
TPANWKSMQQWAKAHNKLFIPSVGPGYIDTRIRPWNGSTTRDRENGKYYDDMYKAAIESG
ASYISITSFNEWHEGTQIEPAVSKKCDAFEYLDYKPLADDYYLIRTAYWVDEFRKARSAS
EDVQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable