Cpin_5607 (C. pinensis DSM 2588 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Cpin_5607
Gene Name:  mannosyl-oligosaccharide 1,2-alpha-mannosidase
Original Clone ID:
BGH47_B03a
Keyword:
partial CDS
Species:
Chitinophaga pinensis DSM 2588
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
CpCD00405524
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1314nts
Open reading frame: 1 to 1314

CAAGCGGAGTTATCGCTAAGCGTCAAACAGGAATTTATCAGATCCTGGGATGCTTACAAA
CGATACGCCTGGGGCCATGATGTATTATTGCCGCAAACGAAAACCTACACGGACTGGTAC
GAACAGCCGATACGTATTTCGCAGATTGATGCATATAGCACCATGAAAGTGATGGGCCTC
CAGAAACAGGCCAGAGACATTGAGAAGTTTGTAACAGATAGTTGCAACTTTGACATAGAC
GTTTACGTAAAGACCTTTGATGTCAATAATCGCGTACTGGGAGGACTCTTATACATGTAC
TCCTGCACACACCAGCCTAAAATACTGGAACGCGCAAGAGACTTTGGAGATCGCCTCTTA
AAAGCTTTCAGATCACCGACAGGAATCCCCTATTACTGGGTCAATCTGAAAACCGGTAAA
GTGAAAGGCGAAAAGGTCAATACCGCAGAAGCAGCCGCTTATACACTTGAAATGGGCATC
CTGAGTTATTACACAAAGGACCCGAAATATTATCAGGCGGCGAGGAAAGCGACAGAAGCC
ATATACGGCAGACGCTCAGCCCTCAACCTTACCGGCAACATCATCAACAATGAAACCGGT
GACTGGCTGGAAACCATCAGTTGTATCGGCGCAGGAGGCAGCGCTTACTATGAAAGTATG
CTGAAAACATGGCTCCTGTTTAAAGATGAAGCACTGAAAGAAATGTGGGAGAAAACCATT
ACAGCCGTACATCAGTATAACGCAGAAGAAAGTGACACCGCAATATGGTACCGCAGAACG
GACATGTATTCAGGGGAAACAAAGAATACAACTGCGACAGGTTACGACGCATTCCTGCCA
GCTCTTTTATGTATCGATAATGATGCAGAACAGGCAGCCAGGGTACACCGGACCTGGAAT
AATCTCTGGAAACAGTATGGGATGATTCCGATGATCTACGACTTCCGCCGGCAGCATATC
ACGGCAGCAGCGTACGACCTGAATCCCGAGATCATCGAGTCAGCCTGGTACCTGTATGAT
TACACAAAAGATACCGCCTATCAGCAGATGGGCAGACAGTACTACAGCGACATATTGCAG
TATTGCAGGGCAGGCGCCGCATTCACTGGCGTAATGGATATCAGAAGTAAACAGCAGCAG
AATAAAATGCCGGTCTATTTCTTTGCAACAACAATGAAATACCTGTACCTGCTCTTTACA
CCAGACAGTGGATTGAATACGCTTGATTACGTATTCACAGCAGAAGGAAACCCGTTTAAA
ACGGATGAATTCAAAGCGGCAGAAACAGCCAGACGTCTGGGCATTAATCAGTAG

Protein sequence predicted based on nucleotide sequence

QAELSLSVKQEFIRSWDAYKRYAWGHDVLLPQTKTYTDWYEQPIRISQIDAYSTMKVMGL
QKQARDIEKFVTDSCNFDIDVYVKTFDVNNRVLGGLLYMYSCTHQPKILERARDFGDRLL
KAFRSPTGIPYYWVNLKTGKVKGEKVNTAEAAAYTLEMGILSYYTKDPKYYQAARKATEA
IYGRRSALNLTGNIINNETGDWLETISCIGAGGSAYYESMLKTWLLFKDEALKEMWEKTI
TAVHQYNAEESDTAIWYRRTDMYSGETKNTTATGYDAFLPALLCIDNDAEQAARVHRTWN
NLWKQYGMIPMIYDFRRQHITAAAYDLNPEIIESAWYLYDYTKDTAYQQMGRQYYSDILQ
YCRAGAAFTGVMDIRSKQQQNKMPVYFFATTMKYLYLLFTPDSGLNTLDYVFTAEGNPFK
TDEFKAAETARRLGINQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable