Gene:
Gene Symbol:
TM0306
Gene Name:
alpha-L-fucosidase
Original Clone ID:
BGH29_B01a
Keyword:
full-length CDS
Species:
Thermotoga maritima MSB8
Type:
cDNA
Vector Name:
pET32-DEST
Format:
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
TmCD00405526
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1347nts
Open reading frame: 1 to 1347
ATTTCTATGAAACCCCGTTACAAACCTGACTGGGAATCTCTGAGGGAACACACAGTACCG
AAATGGTTCGACAAGGCGAAATTCGGGATCTTCATTCACTGGGGGATTTACTCTGTTCCG
GGATGGGCGACGCCCACCGGAGAACTCGGTAAAGTGCCGATGGATGCCTGGTTCTTCCAG
AATCCGTACGCAGAGTGGTACGAAAATTCCCTCAGGATCAAGGAGAGTCCCACCTGGGAA
TACCACGTGAAGACCTACGGAGAAAATTTCGAGTACGAGAAGTTTGCGGATCTTTTCACC
GCAGAGAAGTGGGATCCACAAGAGTGGGCTGATCTCTTCAAAAAAGCAGGAGCGAAGTAC
GTGATACCGACAACGAAACACCACGATGGATTTTGTCTGTGGGGGACGAAATACACAGAT
TTCAACTCCGTGAAGAGAGGACCGAAGAGAGATCTCGTAGGAGATCTTGCAAAAGCCGTA
AGAGAAGCAGGATTGAGATTTGGAGTGTACTACTCAGGAGGTCTGGACTGGCGCTTCACG
ACCGAGCCGATAAGATACCCCGAGGATCTCTCCTACATCAGGCCGAACACTTACGAGTAC
GCAGATTATGCCTACAAACAGGTCATGGAACTTGTGGATCTGTACCTTCCCGACGTTCTC
TGGAACGACATGGGCTGGCCGGAGAAAGGAAAGGAAGACCTGAAGTATCTCTTCGCTTAC
TACTACAACAAACATCCAGAAGGTTCTGTGAACGACAGGTGGGGAGTGCCGCACTGGGAT
TTCAAAACGGCCGAGTACCACGTGAACTATCCGGGGGATCTGCCGGGCTACAAATGGGAG
TTTACGAGGGGAATAGGGCTCTCTTTTGGATACAACCGAAACGAGGGGCCGGAACACATG
CTCTCTGTTGAACAGCTCGTCTACACACTCGTGGACGTTGTGAGCAAGGGAGGAAATCTC
CTTTTGAACGTTGGGCCAAAGGGTGACGGAACGATTCCGGATCTGCAAAAAGAAAGACTC
CTGGGCCTTGGTGAATGGCTGAGAAAGTACGGAGATGCCATCTACGGTACTTCTGTCTGG
GAAAGGTGCTGTGCGAAGACTGAGGATGGAACAGAGATCAGGTTCACCAGAAAATGTAAC
AGAATCTTTGTCATCTTTCTCGGTATCCCGACCGGAGAAAAAATTGTAATTGAGGATCTC
AATCTATCAGCAGGGACAGTGAGACATTTCCTGACGGGGGAGAGATTGAGCTTCAAAAAT
GTGGGAAAGAACCTGGAAATCACAGTACCCAAAAAGCTCCTTGAAACAGACAGCATAACA
CTCGTGTTGGAGGCGGTGGAAGAATAG
Protein sequence predicted based on nucleotide sequence
ISMKPRYKPDWESLREHTVPKWFDKAKFGIFIHWGIYSVPGWATPTGELGKVPMDAWFFQ NPYAEWYENSLRIKESPTWEYHVKTYGENFEYEKFADLFTAEKWDPQEWADLFKKAGAKY VIPTTKHHDGFCLWGTKYTDFNSVKRGPKRDLVGDLAKAVREAGLRFGVYYSGGLDWRFT TEPIRYPEDLSYIRPNTYEYADYAYKQVMELVDLYLPDVLWNDMGWPEKGKEDLKYLFAY YYNKHPEGSVNDRWGVPHWDFKTAEYHVNYPGDLPGYKWEFTRGIGLSFGYNRNEGPEHM LSVEQLVYTLVDVVSKGGNLLLNVGPKGDGTIPDLQKERLLGLGEWLRKYGDAIYGTSVW ERCCAKTEDGTEIRFTRKCNRIFVIFLGIPTGEKIVIEDLNLSAGTVRHFLTGERLSFKN VGKNLEITVPKKLLETDSITLVLEAVEE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET32-DEST
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal Trx, 6xHis and S-tags; Thrombin and enterokinase cleavage sites; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
None
Size (bp):
7611
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
death cassette
ccdB
ccdB death cassette (removed when gene insert is present)
5931
7236
promoter
T7
T7 promoter
5120
5136
protease cleavage site
Thrombin
Thrombin cleavage site
5583
5600
protease cleavage site
Enterokinase
Enterokinase cleavage site
5667
5681
recombination site
attR1
recombination site
5693
5821
recombination site
attR2
recombination site
7277
7401
repressor protein gene
LacI
Lac repressor
3647
4606
selectable marker
CmR
chloramphenicol resistance gene (removed when gene insert is present)
5930
6589
selectable marker
AmpR
ampicillin resistance gene
598
1458
tag
TRX
N-terminal TRX tag
5208
5534
tag
His
N-terminal 6xHis tag
5556
5573
tag
S-tag
N-terminal S tag
5535
5552
trxn regulatory element
lac operator
Lac operator
5139
5163
trxn termination sequence
T7 term
T7 terminator
7525
7586
