glu13A (C. japonicus Ueda107 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  glu13A
Gene Name:  oligo-1,6-glucosidase glu13A
Original Clone ID:
BGH13_B04a
Keyword:
full-length CDS
Species:
Cellvibrio japonicus Ueda107
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
CjCD00405530
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1620nts
Open reading frame: 1 to 1620

ACGACAACCCCCTGGTGGCGCGGCGCGGTTATTTATCAGGTATACCCCCGCAGTTTGATG
GATACCAATGGCGATGGCATTGGTGATATTCCCGGCATTATCAAAAAGCTCGACTACATC
GCCAGCCTGGGTGTGGACGCCATTTGGGTATCGCCATTTTTCAAGTCGCCGATGAAAGAC
TTCGGTTACGACATTAGTGACTATCGGGATATTGATCCCATCTTTGGAACCCTGAGTGAT
TTTGATGAGCTGATCAGCAAAGCCCATGCGCGCAATATCAAGATTATTATCGATCAGGTG
CTCAGCCATACCTCGGACCAGCATGCCTGGTTTTTGGAGAGCCGCGAGTCGCGCGACAAC
CCGAAAGCTGATTGGTATGTGTGGGTAGATCCTCAACCCGACGGAACCCCCCCCAACAAT
TGGTTGGCAATTTTTGGGGGCTCTGCCTGGGAGTGGGAGCCGCGTCGTTGTCAATATTAC
CTGCATAACTTTTTGCGTACCCAGCCAGATTTAAATTACCACTGTCCGCAAGTGCGCGAG
CAAATTTTGCAGGAAGTGGAGTTCTGGTTGAAACGCGGCGTGGATGGTTTGCGCCTTGAT
GCCATTAACTTCTGCTTTCACGATAAGGAATTGCGTTCCAATCCTGCCAAGCCGGAAGCA
GAGCGTAAGGGGCGAGGCTTTAAGGAAGACAACCCCTACGCATTCCAGAAACACATATTT
GATAATACCCGCCCGGAAAACCTGGCTTTCCTGGAGTCCTTGCGCCAGTTAATGGATCGT
TATCCCGGGACGGTAACCCTCGGTGAAATTTCAGCCGAGGACTCCCTGAAAACCATGGCG
GAATACACCTCCGGTAACGACCGTTTGCATATGGCGTATAGCTTTGAACTGCTGGTCGAT
AAGTTATCCGGCAACTATATCCGCAATACGGTGGAAACCCTGGAAGCCAATTTGGCTGAG
GGCTGGCCCTGTTGGTCTATAGGCAACCACGATGTGGTGCGATTTATGTCGCGTTGGGGC
GGTGAGGCACAATCCCCCGAACTCGCCAAAACCCTCAATGCCATGCTGCTTTCATTGCGC
GGCAGTGTGTGTAGCTATCAGGGGGAAGAACTGGGTTTGACAGAAGCGGATATCCAGCAG
CATGAGTTGCAAGATCCCTATGGTATTACCTTCTGGCCTCGCTTCAAGGGGCGCGATGGA
TGTAGAACGCCAATGCCCTGGGACTCATCCCTGGAATACGCGGGATTTTCCAGTGCAAAA
CCATGGCTACCCGTATCGGCAAAACACCTGGCGCTCTCCATCGATAAACAGGAAAGCAAT
CAGGCATCTGTGCTGAACGCCTATCGCCATTTTATGCAGTGGCGTAAAACGCAAGCTGCC
CTGCGCTTTGGTGGCATTGAATTTCTCTCCAGTAGTGAATCTCATTTGGTGTTTGTTCGT
CGCCATGGCAATGAGGCGCTATTGTGTGGATTTAATTTCAGTGCCCAGCCTGCCGTTATT
GAGTTGGGACAAGTGTTGGGCCTGGAGTCAATAACCGGCCATGGTTTTGCAACGCAATCC
TCCCGGTCAAACAATATTGGTTTGCCGGGGTATAGCGCGTTTTTTGCCAGCCTTGTCTAG

Protein sequence predicted based on nucleotide sequence

TTTPWWRGAVIYQVYPRSLMDTNGDGIGDIPGIIKKLDYIASLGVDAIWVSPFFKSPMKD
FGYDISDYRDIDPIFGTLSDFDELISKAHARNIKIIIDQVLSHTSDQHAWFLESRESRDN
PKADWYVWVDPQPDGTPPNNWLAIFGGSAWEWEPRRCQYYLHNFLRTQPDLNYHCPQVRE
QILQEVEFWLKRGVDGLRLDAINFCFHDKELRSNPAKPEAERKGRGFKEDNPYAFQKHIF
DNTRPENLAFLESLRQLMDRYPGTVTLGEISAEDSLKTMAEYTSGNDRLHMAYSFELLVD
KLSGNYIRNTVETLEANLAEGWPCWSIGNHDVVRFMSRWGGEAQSPELAKTLNAMLLSLR
GSVCSYQGEELGLTEADIQQHELQDPYGITFWPRFKGRDGCRTPMPWDSSLEYAGFSSAK
PWLPVSAKHLALSIDKQESNQASVLNAYRHFMQWRKTQAALRFGGIEFLSSSESHLVFVR
RHGNEALLCGFNFSAQPAVIELGQVLGLESITGHGFATQSSRSNNIGLPGYSAFFASLV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable