pRL100282 (R. leguminosarum bv. viciae 3841 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  pRL100282
Gene Name:  glyosyl hydrolase
Original Clone ID:
BGH39_B01a
Keyword:
full-length CDS
Species:
Rhizobium leguminosarum bv. viciae 3841
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
RlCD00405531
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1860nts
Open reading frame: 1 to 1860

CGTGAACGTACGCGAATAAGCGTTGATGCCAGTGAAGCGGTACGCCCGTTCAACCGGTTC
TGGCGCGGCACCGGCTTTTCGCCGGCCGAGCTGCTGCTCGAGCCCGAGATGCGCCAGATG
CTTGCCTATATCGGTGGCCTGCCGAATGAGGGCATCAAGTTCCTGCGGGTGCATTATCTC
TACAATCTGCTGAGCGCGAAGGGCGAGGTCGGCTACGACTGGTCGCTGCTCGACCGCGCG
CTCGACGTGATGATCGAACACCGCCTGAAGCCGTTCTTCGAACTGATGGGCAACCCATCC
GGCCTGTTCACCGACTATGAGGACATGGATCAGGTCAGGCGCTGGCGGGATCTCGTGACG
GCAACGGTCGACCGTTATGGCGCGCGTTACGGCATGGACGAATTGCGCACATGGTATTTC
GAAACGACGAACGAGGCCGACTCCGGCTGGTGGACCTACGGGATCAAGGGTTACACCAAC
TACTACGACGCCTGCGTCGCGGGGCTCGATGCCATCGATCCCACCTTGCCGATGGGCGGA
CCCGGAACCGCCCGCACGCTCTCGCCGATCTTTCGCGCCCTGATGGCCCATTGCGACAGC
GGCACGAGCTGCCTCAGCGATGACGGCCCGCCGCGCATCGATTACATCTCGATCCACGAA
AAGGGCGTCAACGGCAGCAAGGAAGACCTGACCCCGAAAACCAACGGTATCGTCGACCGC
ACTTTGCTCGTCGTCGACTATCTGAAGGAGCATCACCCGCGCCTGGCGGGCTTGCCCATC
GTCAACGATGAATGCGATCCGCAGCTTGGCTGGAGCGATCATCATAGCTGGCACGGCAAA
GCCTATTACGCCGGTATCATCGCCCGCATCATCGAACAGCACGACCGGCGCATCATCGCC
CCGAAAGCGGCGAATTTCACCTTCCTCAGCAATGACCACGCCTTTATCGGCGGCTGGAGC
CAGCGCACGATCTTTGCCTATTTCGGTTCGCGCAATTTCACCAAGGCGCAGTGGGAGCAC
AAGACCAATCTGGATATGCTGGTCACCGATGTCGACAGGGCGCCGCCCTTCGACATCATC
AAGAAGCCCGGCCTCACATCGATGGAACTGCTGGCAACGCTTGGCGATACCGTTTGCAAG
GTGACGGCCGAACCGCCGCTTGCGCCCGACCAGGATGGCCTGGCGATCCTGCCGACGCGG
CTGCCGGGCGGCGGTGTTTCCATCAGCCTCATCCATAGCGTCGATGCCATCAACCGCTCG
GGCCGGACCGCCGTCCGTCTCGAGGTGAGCGGCTTGGTTCCCGGCCGCCACGCGCTTTGC
CTGCTGCGCATCGACGAGGAATTCACCAATCCGATGGAGGTCTGGGAAGCCCAGCACGAT
GAAAGCAACCCGCGTGGGCTCTTCGAGCCGGTCGGCGCACCGCCTGCACCTGATGAAGCG
CAGTTTGCCGAAATGCGCCGCGCGCAGGAGCCCGCCCTGCTGCATCCGATCAGCGTCGTC
GCGTGCGACGAAGGCCGGATCAGTGTCGACCTGGATGTGCCATTGCCGTCGCTGACGCAG
GTGCTTGTCGTTCCCGATATCGGCGTGCCACCGGCGGCACCGAGCGGCCTTGTCGTCGAA
CGCTATCTCGGCCTCGGCGGTCGGGAGGAGCGCATGCTGTTCTGGGCTGCCGGCGATGCC
AGCCCGGCCATCTTCTACGATGTCCTGGTCAGCACCGATGGCGCGGCTTTTGAAAAGGTC
AGTTCAGCGCCGCTGATCTCGACGGCATTCCTGCACATGTCGCCGCCGCAGGGCGTGCGT
TATGCGGTGCGCGCCCGCGATGCATTCGGTCGCCGCAGCGAGCTTTGTCTCTCCCGCTAG

Protein sequence predicted based on nucleotide sequence

RERTRISVDASEAVRPFNRFWRGTGFSPAELLLEPEMRQMLAYIGGLPNEGIKFLRVHYL
YNLLSAKGEVGYDWSLLDRALDVMIEHRLKPFFELMGNPSGLFTDYEDMDQVRRWRDLVT
ATVDRYGARYGMDELRTWYFETTNEADSGWWTYGIKGYTNYYDACVAGLDAIDPTLPMGG
PGTARTLSPIFRALMAHCDSGTSCLSDDGPPRIDYISIHEKGVNGSKEDLTPKTNGIVDR
TLLVVDYLKEHHPRLAGLPIVNDECDPQLGWSDHHSWHGKAYYAGIIARIIEQHDRRIIA
PKAANFTFLSNDHAFIGGWSQRTIFAYFGSRNFTKAQWEHKTNLDMLVTDVDRAPPFDII
KKPGLTSMELLATLGDTVCKVTAEPPLAPDQDGLAILPTRLPGGGVSISLIHSVDAINRS
GRTAVRLEVSGLVPGRHALCLLRIDEEFTNPMEVWEAQHDESNPRGLFEPVGAPPAPDEA
QFAEMRRAQEPALLHPISVVACDEGRISVDLDVPLPSLTQVLVVPDIGVPPAAPSGLVVE
RYLGLGGREERMLFWAAGDASPAIFYDVLVSTDGAAFEKVSSAPLISTAFLHMSPPQGVR
YAVRARDAFGRRSELCLSR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable