SCO1423 (S. coelicolor A3(2) )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SCO1423
Gene Name:  glycosyl transferase
Original Clone ID:
BGT02_T03b
Keyword:
full-length CDS
Species:
Streptomyces coelicolor A3(2)
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
ScCD00405538
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 906nts
Open reading frame: 1 to 906

AACGACGGCGACGGGACGCCCGCGGCTGCGGCGCGGGAGAGGCGGTTCGGCCCGCTGGGC
ACGGCCCTGGTGATCATCCCGACCTACAACGAGGCGGAGAACATCAAGGCGATCGTCACG
CGTGTGCGCGAGGCCGTCCCCGAGGCACACGTGCTCGTGGCCGACGACAACAGCCCCGAC
GGCACCGGCAAGCTCGCCGACGAACTGGCGGCCGCGGACGACCACGTCCAGGTCATGCAC
CGCAAGGGCAAGGAAGGGCTGGGCGCCGCCTACCTCGCCGGCTTCCGCTGGGGCCTGGAG
CACGGCTACGGCGTGCTGATCGAGATGGACGCCGACGGCTCCCACCAGCCCGAGGAACTG
CCCCGTCTGCTGACCGCCCTCAAGGGCGCCGACCTCGTCCTCGGCTCGCGCTGGGTGCCC
GGCGGCCGGGTGGTCAACTGGCCCAAGTCCCGCGAGATCATCTCGCGTGGCGGCAGCATG
TACTCGAGAATCGCGCTCGACCTGCCGCTGCGCGACATCACCGGCGGCTACCGCGCCTTC
CGCCGCGAGACCCTGGAGGGGCTCGGCCTCGAGGAGGTCGCCTCCCAGGGGTACTGCTTC
CAGGTCGACCTCGCCCGCCGGGCGATCAAGGCCGGCTATCACGTCGTGGAGGTGCCGATC
ACGTTCGTGGAGCGGGAACTCGGCGACTCCAAGATGAGCCGCGACATCCTGGTGGAGGCC
CTGTGGCGGGTCACCACGTGGGGCGTGGGGGAGAGGGTCGGCAAGGTGCTCGGCCGCGAC
AGGCGGTCCGGCACGGCGGCCGACGGGCAGACGGCCTCGAGCGGGACCGACGGGCGGACG
GCCTCGAGCGACAGGTCGACGGACTCCCTCGACGAGTCGGCGAAGGCGTCCGACGCCAAG
GCCTAG

Protein sequence predicted based on nucleotide sequence

NDGDGTPAAAARERRFGPLGTALVIIPTYNEAENIKAIVTRVREAVPEAHVLVADDNSPD
GTGKLADELAAADDHVQVMHRKGKEGLGAAYLAGFRWGLEHGYGVLIEMDADGSHQPEEL
PRLLTALKGADLVLGSRWVPGGRVVNWPKSREIISRGGSMYSRIALDLPLRDITGGYRAF
RRETLEGLGLEEVASQGYCFQVDLARRAIKAGYHVVEVPITFVERELGDSKMSRDILVEA
LWRVTTWGVGERVGKVLGRDRRSGTAADGQTASSGTDGRTASSDRSTDSLDESAKASDAK
A*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable