mll0582 (M. japonicum MAFF 303099 )
In pET32-DEST (Trx/His/S-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  mll0582
Gene Name:  hypothetical protein
Original Clone ID:
BGT25_T02a3
Keyword:
partial CDS
Species:
Mesorhizobium japonicum MAFF 303099
Type:
cDNA
Vector Name:
pET32-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term Trx tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
MlCD00405540
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1686nts
Open reading frame: 1 to 1686

GTCAATGAGCCGCCGAAACTTGGGGCGTTCGGGACCGAGAGCTTGATCGACCAGCACAGG
CTTGTCCGGCAGCGGTCGTTGCGGTCGCGCATCGAGGGCACGCCGCGCATCCTGGTGACA
ATCCTCGCAAAGCAGAAAGAGCCGGCACTGCCGCTGTACCTCGAGTGCATCGAGGCGCTC
GACTACCCCAAGGCGTCGATCGTCCTGTACATCAGGACAAACAACAACACCGACAGGACC
GAGCACATACTGCGCGAATGGGTGGAGCGCGTTGGTCACCTCTATGCCGCGGTTGAATTC
GATGCCTCCAACGTGGCCGATAGAGTCGAGCAGTTTGGCGAACACGAATGGAACGAGACG
CGCTTCAGGGTGCTTGGCCGAATCCGCAACATAAGTTTGCGGAAAACTCTCGAACACAGC
TGCGATTTCTATTTCGTTGCCGACGTCGATAATTTCGTGCGACCGGCCACGCTACGCGAG
CTTGTCGCGCTCGACGTGCCGATCGTCGCTCCCCTGCTTCGCTCCATCTCTCCCGGGCAA
TACTATTCGAACTACCACGCCGAAATCGACGCCAACGGCTACTATATGCAGTGCGATCAA
TATGGGTGGGTGCTCAACCGCCACGTGCGCGGCATTATCGAGATGCCGCTTGTGCACTGC
ACCTATCTTGTCCGCGCCGACGTACTGCCCGAACTGACATACGAGGACGCGACATCGCGC
TACGAGTACGTGATCTTTGCCGACAGCGCCCGCAAGGCCGGCATCGTCCAGTATATGGAT
AACCGACAAGTCTATGGCTACATCACCTTCGGCGATGGCCAATATTATGTGAGTGACGGG
ATCGAGCGCGCCCGAGCGCTGTTGCACGGTGCCGGCGACACGCCGGTATTCACTGCCGCC
ACGCCTCCGGGCGCCGTACCGATGCCAGATCGACATGTCGAGCCGCCGCAAATTCACCTA
ATAAACTTGGACAGCAGCGTCGAGCGCTGGCGCAAATATCAAGATAGGAATCCTCATCTA
GCCGCCAATACAATTCGGGTATCGGCCGTCGACGGAGCATCCCTCGATAGGTCGACCTTG
CAAGCCTTGGTGGACGATGGAGTGATTGCCGAGGATTGCGGGTATCTGCCGGGCGCATTG
GGATGTGCTCTATCGCACATCAACTTGTGGAAGTTAGCGGTATCCGAGAAGCGGCCAATT
ACCATTTTCGAGGACGACGTCTACTCGTCGTTCAACTTTCTTGAGGAGTCGACTCGCATT
CTGTCCATGGCTCCAGAGAACTGGGATATGGTAAAGTGGGGATTCAATTTCGACCCTCTA
TTCCTGTGGTTGAACTTCGATTTCTCCAAAGCCAAGCTGGAGTTCTACAACCGACAACAC
AAACAGATTCCGGCCCAATTTCAATCTGAAACCTATCCGCGATCTCTTATCCGACTAGTC
CATTCCTTTGGTGCAATGGCTTATTCAGTAACTCCAAGGGGAGCTCAAGTACTTCTTGAA
AAATGCCTACCTCTTCGAAAGCGGCTTATTCCGTTTCCTGGGACTGGAGTGGTCCTCGAG
GATGAGGGCATCGACTGCGCAATGTCCGCGGTGTATGACTCGATGCATGCGTTCGTCTGC
ATGCCGCCACTGGTCATTCACGACGACGAGCAGGCATCAGATCGGGAAGTCGCGAATCGG
CAGTAG

Protein sequence predicted based on nucleotide sequence

VNEPPKLGAFGTESLIDQHRLVRQRSLRSRIEGTPRILVTILAKQKEPALPLYLECIEAL
DYPKASIVLYIRTNNNTDRTEHILREWVERVGHLYAAVEFDASNVADRVEQFGEHEWNET
RFRVLGRIRNISLRKTLEHSCDFYFVADVDNFVRPATLRELVALDVPIVAPLLRSISPGQ
YYSNYHAEIDANGYYMQCDQYGWVLNRHVRGIIEMPLVHCTYLVRADVLPELTYEDATSR
YEYVIFADSARKAGIVQYMDNRQVYGYITFGDGQYYVSDGIERARALLHGAGDTPVFTAA
TPPGAVPMPDRHVEPPQIHLINLDSSVERWRKYQDRNPHLAANTIRVSAVDGASLDRSTL
QALVDDGVIAEDCGYLPGALGCALSHINLWKLAVSEKRPITIFEDDVYSSFNFLEESTRI
LSMAPENWDMVKWGFNFDPLFLWLNFDFSKAKLEFYNRQHKQIPAQFQSETYPRSLIRLV
HSFGAMAYSVTPRGAQVLLEKCLPLRKRLIPFPGTGVVLEDEGIDCAMSAVYDSMHAFVC
MPPLVIHDDEQASDREVANRQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable