Cthe_0212 (A. thermocellus ATCC 27405 )
In pET16-DEST (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Cthe_0212
Gene Name:  beta-glucosidase
Original Clone ID:
BGH01_B01b
Keyword:
full-length CDS
Species:
Acetivibrio thermocellus ATCC 27405
Type:
cDNA
Vector Name:
pET16-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term 6xHis tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
CtCD00405544
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1413nts
Open reading frame: 1 to 1413

TTTCCTCTAGGTTATAATTATATTATTACACTGTTTGCAAATAATATCTTAAAGGGTGTG
GTAAACATGTCAAAGATAACTTTCCCAAAAGATTTCATATGGGGTTCTGCAACAGCAGCA
TATCAGATTGAAGGTGCATACAACGAAGACGGCAAAGGTGAATCTATATGGGACCGTTTT
TCCCACACGCCAGGAAATATAGCAGACGGACATACCGGCGATGTTGCATGCGACCACTAT
CATCGTTATGAAGAAGATATCAAAATAATGAAAGAAATCGGTATTAAATCATACAGGTTT
TCCATCTCATGGCCCAGAATCTTTCCTGAAGGAACAGGTAAATTAAATCAAAAGGGACTG
GATTTTTACAAAAGGCTCACAAATCTGCTTCTGGAAAACGGAATTATGCCTGCAATCACT
CTTTATCACTGGGACCTTCCCCAAAAGCTTCAGGATAAAGGCGGATGGAAAAACCGGGAC
ACCACCGATTATTTTACAGAATACTCTGAAGTAATATTTAAAAATCTCGGAGATATCGTT
CCAATATGGTTTACTCACAATGAACCCGGTGTTGTTTCTTTGCTTGGCCACTTTTTAGGA
ATTCATGCCCCTGGGATAAAAGACCTCCGCACTTCATTGGAAGTCTCGCACAATCTTCTT
TTGTCCCACGGCAAGGCCGTGAAACTGTTTAGAGAAATGAATATTGACGCCCAAATTGGA
ATAGCTCTCAATTTATCTTACCATTATCCCGCATCCGAAAAAGCTGAGGATATTGAAGCA
GCGGAATTGTCATTTTCTCTGGCGGGAAGGTGGTATCTGGATCCTGTGCTAAAAGGCCGG
TATCCTGAAAACGCATTGAAACTTTATAAAAAGAAGGGTATTGAGCTTTCTTTCCCTGAA
GATGACCTGAAACTTATCAGTCAGCCAATAGACTTCATAGCATTCAACAATTATTCTTCG
GAATTTATAAAATATGATCCGTCCAGTGAGTCAGGTTTTTCACCTGCAAACTCCATATTA
GAAAAGTTCGAAAAAACAGATATGGGCTGGATCATATATCCTGAAGGCTTGTATGATCTG
CTTATGCTCCTTGACAGGGATTATGGAAAGCCAAACATTGTTATCAGCGAAAACGGAGCC
GCCTTCAAAGATGAAATAGGTAGCAACGGAAAGATAGAAGACACAAAGAGAATCCAATAT
CTTAAAGATTATCTGACCCAGGCTCACAGGGCAATTCAGGACGGTGTAAACTTAAAAGCA
TACTACTTGTGGTCGCTTTTGGACAACTTTGAATGGGCTTACGGGTACAACAAGAGATTC
GGAATCGTTCACGTAAATTTTGATACGTTGGAAAGAAAAATAAAGGATAGCGGCTACTGG
TACAAAGAAGTAATCAAAAACAACGGTTTTTAG

Protein sequence predicted based on nucleotide sequence

FPLGYNYIITLFANNILKGVVNMSKITFPKDFIWGSATAAYQIEGAYNEDGKGESIWDRF
SHTPGNIADGHTGDVACDHYHRYEEDIKIMKEIGIKSYRFSISWPRIFPEGTGKLNQKGL
DFYKRLTNLLLENGIMPAITLYHWDLPQKLQDKGGWKNRDTTDYFTEYSEVIFKNLGDIV
PIWFTHNEPGVVSLLGHFLGIHAPGIKDLRTSLEVSHNLLLSHGKAVKLFREMNIDAQIG
IALNLSYHYPASEKAEDIEAAELSFSLAGRWYLDPVLKGRYPENALKLYKKKGIELSFPE
DDLKLISQPIDFIAFNNYSSEFIKYDPSSESGFSPANSILEKFEKTDMGWIIYPEGLYDL
LMLLDRDYGKPNIVISENGAAFKDEIGSNGKIEDTKRIQYLKDYLTQAHRAIQDGVNLKA
YYLWSLLDNFEWAYGYNKRFGIVHVNFDTLERKIKDSGYWYKEVIKNNGF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable