Haur_4334 (H. aurantiacus DSM 785 )
In pET16-DEST (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Haur_4334
Gene Name:  glycoside hydrolase
Original Clone ID:
BGH31_B04a
Keyword:
full-length CDS
Species:
Herpetosiphon aurantiacus DSM 785
Type:
cDNA
Vector Name:
pET16-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term 6xHis tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HaCD00405568
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1848nts
Open reading frame: 1 to 1848

GCATACACCCTCGAATCAACAGGCTTATTGGTATTTCAACGTGATGACGAAGAAGTTTTG
ACGATTCAGCCCTACTTGCAGGCTTATGGCTCGCAGTTTGCCGCATTAGGTGTACAAACC
AAGCCTGATACGGTGCAATACACTAGTTTCGATAACCAGACCTTTGAGCTTAATCTTGAA
ACGACGGCAACCGGCTATCAGTTGGTGTTTCACGTGAAACATCAGGTTGCTCAAATTGGT
TTAGCAATCGGCGTACAACCAGCAGGCTCGTGGTATGGTATGGGCGAACGGGTGATTCAA
AGTTGGCCGCTCAACTTGGCTGGTGTGCAAAGCCAACCATTTATGACCTATGATCACGCC
AATGATGGCACACTCAATATTGTTACGCCAGCCTGGATTGGCGCGAATGGCGTGGCTTTT
ATCGTGGCCGAAGATACAGGCCCGTTGCATGTCACAATCGATAGCGATCCAGCAGGTGTG
ATTCGGCTGGTGCAGTTTCCTTCGCCAACCCCATTTGGCGCGGGCTTGGATGGCAGCGAA
ACCCATTATGAAGGCACGCGCTTGGTGCTCGATCTACTGATTGCTGAAAATGTCAGCGTT
GCCGCTCAACACGTCATTCAACAATTGGGCTACCCCAAGGCGGCTCCACCCTTGGCAATG
TTTAGCAAGCCAATTTGGACAACCTGGGCACGCTATAAAATGGATATCGATCAAGCTCAA
ACGCTGGCGTTTGCCCAAGAAATTATCGACCAACAGTATCCATATTCGGTGCTAGAGATC
GACGATCGCTGGCAAACTGCTTATGGCGATTTAGAATTCGATCGGCGCAAGTTTCCCGAT
CCCAAGGCTATGGTCGATCAATTGCATCAGCTTGGCTATAAAGTAACCTTGTGGATTCCA
CCATTTTTCGATCCAAAGAGCGCGGCTTTTGCTGAAGCTACTGCTAATGGCTATTTGGTC
AAACATCCCGCCAACGATCAACCGTATTTGACCCGTTGGTGGCAAGGTTGGGGCGGTTTG
TTGGATGTCTCGAATCCTGCTGCCTTGGCTTGGTGGCAAGCAGGTTTGGAGCGCTTGCAA
ACCTTGTATGGCATCGATGGTTTTAAATTTGACGGCGCTGAAGGGAATTTTCTACCCGCC
GAAGCCAAAACCCATCTGCCGATGACTCCCAACCAATATAGTGATCGCTATGTGGCTTTT
GTGGCCAAATCGTGGCAATGGACAGAGGTACGAACTGGTTGGCGCTCGCAACAGCAACCA
ATCTTCTTCCGCGAATGGGACAAATGGAGTCGTTGGGGCATGGACAATGGCTTGCATGCG
GTCGTTACCCAAGCGCTTGCGATGAGCGTGATCGGTTATCCCTATGTGCTGCCCGATATG
ATCGGCGGCAACGCCTATAACGGTGAATTTCCCGAGCGCGAGTTGCTGATTCGTTGGACG
CAAGTCACGGCATTATTGCCAGCGATGCAATTTTCAATCGCGCCATGGCAGTACGATGTA
GAAACCAGCCAGATTTGCCAGCGCTATGCTCAATTGCACGCTGAGCTAGAGCCATACATT
GCCGAATTGGTGCAAGCGACCATCACCGATGGTACGCCTTTAGTTCGGCCTTTGTGGTGG
CACTATCCCGACGATGCCAGCACGCGCTTTATTGGTGATCAATGGTTGTTTGGCGAGCAA
TACTTGGTTGCGCCAATGCTCCAAGCCAACCACTACCAACGTGACATTTATTTGCCTGAA
GGTGGCTGGCGCGATTATTGGACTGGCGAGAAATTCGAGGGTGAAACCTGGCTCTACAAT
TATCCTGCGCCCTTAGAAACCCTGCCGTTGTTCGAGCGGCTGTGGTAG

Protein sequence predicted based on nucleotide sequence

AYTLESTGLLVFQRDDEEVLTIQPYLQAYGSQFAALGVQTKPDTVQYTSFDNQTFELNLE
TTATGYQLVFHVKHQVAQIGLAIGVQPAGSWYGMGERVIQSWPLNLAGVQSQPFMTYDHA
NDGTLNIVTPAWIGANGVAFIVAEDTGPLHVTIDSDPAGVIRLVQFPSPTPFGAGLDGSE
THYEGTRLVLDLLIAENVSVAAQHVIQQLGYPKAAPPLAMFSKPIWTTWARYKMDIDQAQ
TLAFAQEIIDQQYPYSVLEIDDRWQTAYGDLEFDRRKFPDPKAMVDQLHQLGYKVTLWIP
PFFDPKSAAFAEATANGYLVKHPANDQPYLTRWWQGWGGLLDVSNPAALAWWQAGLERLQ
TLYGIDGFKFDGAEGNFLPAEAKTHLPMTPNQYSDRYVAFVAKSWQWTEVRTGWRSQQQP
IFFREWDKWSRWGMDNGLHAVVTQALAMSVIGYPYVLPDMIGGNAYNGEFPERELLIRWT
QVTALLPAMQFSIAPWQYDVETSQICQRYAQLHAELEPYIAELVQATITDGTPLVRPLWW
HYPDDASTRFIGDQWLFGEQYLVAPMLQANHYQRDIYLPEGGWRDYWTGEKFEGETWLYN
YPAPLETLPLFERLW*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable