Gene:
Gene Symbol:
CPF_0285
Gene Name:
endo-beta-N-acetylglucosaminidase
Original Clone ID:
BGH85_B02b2
Keyword:
partial CDS
Species:
Clostridium perfringens ATCC 13124
Type:
cDNA
Vector Name:
pET16-DEST
Format:
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term 6xHis tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
CpCD00405571
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 2025nts
Open reading frame: 1 to 2025
ACACCTAAACAAGATACAAGTGTTGTAAATTATGTGAAAACTCAAGAAGAAACTTCAAAC
TCAATTCAGAATCAACCAATCTCATCTTATTGGTATCCAGAAGATTTATTAAAATGGAGT
GCTAACAATGATAAAGATGCAAAATTTAATAAAAGCACAGTTCCATTAGCAAAAAGAGTC
GAAAAGGATAAACTTGATACTATTAATGACACTCAAAATAAAGATGTTAAAGTTGTAGCA
ATTTCAATAATGAATGCTAATACTAGTGGCAATCCATCACAAGGTTCAAATAAATTTAGT
GCTAATACATTCTCTTATTGGCAATACATAGATAAATTAGTTTACTGGGGTGGATCAGCT
GGAGAAGGATTAATAGTTCCTCCAAGTCCAGATGTTACTGACTCAGCACACAGAAATGGG
GTTCCTGTTTTAGGTACTGTATTCTTCCCTATGACAGCTCATGGTGGAAAAATGGAATGG
TTAAATAAATTCCTTGAAAAAGATTCTAATGGAAACTTCCCTATAGTAGATAAGTTAATT
GAAGTTGCAGAAAACTATGGCTTTGATGGTTGGTTCATAAATCAAGAAACAGAAGGAACT
GAACAAGAACCTTTAACGCCTGAACATGCTAAGCTTATGCAAGAATTAATAAAGCAATTT
AAAGCTAAGTCTAATGGAAAATTTGAAATTATGTGGTATGATTCCATGACAAAAGATGGG
GATATGGATTGGCAAAATGCTTTAACTGATAAAAATGAATATTTCTTATTAGATGGAGAT
AAAAACAAAGTTGCTGATAGTATGTTCTTAAATTTCTGGTGGACATATAATAGTTTAAAA
GATAAAGATTTATTAAGAGTATCTAATGAAAAAGCTAAAGAAATAGGAATAAACCCTTAT
GACTTATATGCTGGAATAGATGTTCAAGCAAATGGATATAAAACTCCTCTTAGATGGGCT
CATTATCAAAGAGATGACCAAGCTCCATTTACTTCATTAGGTTTATATTGTCCAAGCTGG
ACTTATTTTGATGCTAAAACTCCAGAACAATTTCAAGAAAACGAAAGTAGATTATGGGTT
AATGAACATGGTGATCCATCAAAGGCAACTAATGCTCAAGGAGTTGATTGGAGAGGAATT
TCAACATACTCTGTTGAAAAAAGTGCTGTAACTAGCCTTCCATTTACAACTAACTTTAAT
ATGGGTAATGGATATGATTTTTATGTTGATGGTAATAAAGTATCTACTCAAGATTGGAAT
AATAGAAGTTTAAATGATATAATGCCAACTTACAGATGGGTTATTTCAAATGAAGAAAAT
AATAATGTAAAAGCTGACATAGATTATACTAATGCTTACTATGGTGGTAACTCAATTAAA
CTTTCTGGTTACCTAGCTGAAGGAAAAGCTTCTACAATAAAACTTTATAGTTCTGATTTG
ACTTTACCTGAAGGTGTACAATTCACTACTACTGCTAAGGCTAATGGAAATACTGTAGAT
ATGGATTTAGTTCTTACATTCCATGATGGTACAGAAACTACTATTTCTGCTGATAAGAAA
CTTGGTACTGATTGGACTAAACTTACTTATGATGTTTCACCATATGTAGGAAAATCAATT
AAAACTATTTCTTATAAACTTTCAAGCCCAGTATGTGTTGATAACTTCTCTGCAAACTTA
GGTAATATAACAATAGAAATTCCTAACTCAAGTTCAAAGGTAAATATATCTAATTCTAAA
TTAAATGATGTTGACTTTAAAGATGGAATATATGCAGGAGCTAGACTTTCATGGAGTCCT
GAAGGAAATTCTTCTGATGTACATCATTATGAAATTTACAGAGTTATGAATGATGGAACT
AAAGTTTTACTAGGTGCAACTCCTAATACAAGTTATTATGTTAGTGATTTAAGAAGAAAT
GATAAAGAAACAAATACTAATTTTGAGGTAATTGCTGTTAACAAAAACTATAACAGAGGA
AATAGTCAAAGTATAAATATGGAATGGCCTGCTTACCCTGCTTAG
Protein sequence predicted based on nucleotide sequence
TPKQDTSVVNYVKTQEETSNSIQNQPISSYWYPEDLLKWSANNDKDAKFNKSTVPLAKRV EKDKLDTINDTQNKDVKVVAISIMNANTSGNPSQGSNKFSANTFSYWQYIDKLVYWGGSA GEGLIVPPSPDVTDSAHRNGVPVLGTVFFPMTAHGGKMEWLNKFLEKDSNGNFPIVDKLI EVAENYGFDGWFINQETEGTEQEPLTPEHAKLMQELIKQFKAKSNGKFEIMWYDSMTKDG DMDWQNALTDKNEYFLLDGDKNKVADSMFLNFWWTYNSLKDKDLLRVSNEKAKEIGINPY DLYAGIDVQANGYKTPLRWAHYQRDDQAPFTSLGLYCPSWTYFDAKTPEQFQENESRLWV NEHGDPSKATNAQGVDWRGISTYSVEKSAVTSLPFTTNFNMGNGYDFYVDGNKVSTQDWN NRSLNDIMPTYRWVISNEENNNVKADIDYTNAYYGGNSIKLSGYLAEGKASTIKLYSSDL TLPEGVQFTTTAKANGNTVDMDLVLTFHDGTETTISADKKLGTDWTKLTYDVSPYVGKSI KTISYKLSSPVCVDNFSANLGNITIEIPNSSSKVNISNSKLNDVDFKDGIYAGARLSWSP EGNSSDVHHYEIYRVMNDGTKVLLGATPNTSYYVSDLRRNDKETNTNFEVIAVNKNYNRG NSQSINMEWPAYPA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET16-DEST
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and a N-terminal 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning.
Comments:
None
Size (bp):
7428
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
death cassette
ccdB
ccdB death cassette (removed in clones with the insert)
6626
6931
promoter
T7
T7 promoter
5230
5245
protease cleavage site
Factor X
Factor X protease cleavage site
5365
5376
recombination site
attR1
Recombination site
5392
5516
recombination site
attR2
recombination site
6972
7096
repressor protein gene
LacI
Lac repressor coding sequence
3761
4720
selectable marker
CmR
chloramphenicol resistance (removed in clones with the insert)
5625
6284
selectable marker
AmpR
ampicillin resistance gene
209
1843
tag
His
N-terminal 10xHis tag
5323
5352
trxn regulatory element
lac operator
lac operator
5249
5273
trxn termination sequence
T7 term
T7 terminator
7155
7216
